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The Short-term Effects of Soil Brought and Subsoil Inversion on Growth and Tissue Nutrient Concentrations of Fraxinus rhynchophylla, Pinus densiflora, and Pinus koraiensis Seedlings in a Nursery (객토와 심토뒤집기 처리가 물푸레나무, 소나무, 잣나무 묘목의 초기 생장과 양분함량에 미치는 영향)

  • An, Ji Young;Park, Byung Bae;Byun, Jae Kyung;Cho, Min Seok;Kim, Yong Suk;Han, Si Ho;Kim, Se Bin
    • Journal of Korean Society of Forest Science
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    • v.104 no.1
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    • pp.43-49
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    • 2015
  • The production of high quality seedlings is a very important phase in silvicultural systems for successful reforestation or restoration. The purpose of this study was to quantitatively measure both growth performances and nutrient responses of Fraxinus rhynchophylla, Pinus densiflora, and Pinus koraiensis seedlings, which are commercially planted in Korea, according to the different types of soil improvement treatments. We applied soil brought (hereafter 'brought'), subsoil inversion (hereafter 'subsoil'), and mixture of brought soil with soil on nursery bed (hereafter 'mixing') in a permanent national nursery. Silt and clay contents were the highest at the subsoil treatment and organic material, soil nitrogen and phosphorus concentrations were the lowest at the brought treatment. The growth of F. rhynchophylla was the lowest at the subsoil treatment, but there were no significant differences among treatments. There were significant differences in only root nutrient concentrations of F. rhynchophylla among treatments: nitrogen, phosphorus, and potassium concentrations were the lowest at the subsoil or brought treatment. Mixing treatment increased N contents with deduction of N concentrations ('dilution') because of more dry weight increase compared with the amount of N uptake. This study suggested mix of brought soil with soil on a nursery bed in a permanently used nursery can economically be an effective technique to improve soil quality.

Irrigation Scheduling Model for Dry Crops (밭작물(作物)의 계획관개(計劃灌漑) 모형(模型) - 토양수분(土壤水分) 변화(變化)를 중심(中心)으로 -)

  • Ahn, Byoung Gi;Kim, Tai Cheol;Cheoung, Sang In
    • Korean Journal of Agricultural Science
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    • v.14 no.1
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    • pp.68-80
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    • 1987
  • This study was carried out to investigate the evapotranspiration and variations of soil moisture contents for soybeans. The relationship between actual evapotranspiration obtained by the water balance equation and estimated evapotranspiration obtained by the soil moisture model was analyzed. The results obtained were summarized as follows; 1. The total amount of actual evapotranspiration of soybeans during growing season was 405.7mm. The total amount of reference crop evapotranspiration of soybeans that was estimated by Pan evaporation and Hargreaves method were 547.8 mm and 586.8 mm, respectively. Crop coefficient during growing season were shown on Table 1. 2. Measured actual evapotranspiration of soybean during growing season was 405.7 mm and estimated actual evapotranspiration by pan evaporation and Hargreaves method were 424.7 mm, and 426.1mm, r3 respectively. 3. The variations of soil moisture content for soybeans were high at 10cm layer, as compared with those at 30cm and 50cm layers. Because discrepancy between the variations of soil moisture content predicted by model and observed by soil moisture meter was still great, it is required to study the consumptive types of soil moisture at each root depth.

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Selection of Optimal Varieties Suitable for Indoor Cultivation Considering the Growth and Functional Content of Agastache Species (배초향의 생장과 기능성 물질 함량을 고려한 실내재배 적합 최적 품종 선정)

  • Do, Jong Won;Noh, Seung Won;Bok, Gwon Jeong;Lee, Hyun Joo;Lee, Jong Won;Park, Jong Seok
    • Journal of Bio-Environment Control
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    • v.29 no.2
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    • pp.202-208
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    • 2020
  • The objective of this study was to select the right species among Agastache in the family Lamiaceae as the result of growth analysis and functional substance analysis. Among 22 species of Agastache, five species (Agastache cana, Agastache foeniculum, Agastache rugosa 'Spike Blue', Agastache rugosa 'Spike Snow', Agastache rupestris) were selected by referring to United States Department of Agriculture (USDA)'s plant hardiness zone area and Korea's one based on USDAs. These plants were cultured at 24 ± 1℃ and 18 ± 1℃ (day and night temperatures, respectively) and 65 ~ 75% relative humidity in a hydroponic culture system for 4 weeks. In terms of growth analysis experiment, stem length, root length, leaf width, leaf area, leaf number, SPAD value, and fresh and dry weights of shoots and roots were measured. The results showed that A. rugosa SS and A. rugosa SB have a higher overall balance value than other species. When compared functional substance value (rosmarinic acid, tilianin, and acacetin) of five species, A. rugosa SS and A. rugosa SB are significantly higher than other species in Agastache. It seems to be directly proportional to the growth analysis results. When considering the growth and functional substance part comprehensively, A. rugosa SS and A. rugosa SB are the most optimal high-value species among Agastache.

Isolation of Nematophagous Fungi against Root-knot Nematode and Their Growth in Vitro (뿌리혹 선충에 대한 기생 천적 진균 분리 및 이들의 생장에 미치는 환경조사)

  • 정미정;김희규
    • Korean journal of applied entomology
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    • v.27 no.3
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    • pp.149-158
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    • 1988
  • Nematophagous fungi were successfully isolated by baited plating, centrifugation technique of soil, and direct isolation from naturally ingested nematodes. Predominant seven fungi isolated were identified as Artheobotrys arthroboteyides, A.conoides, A. oligospora, Dactylella lobata, Fusatium oxysporum, Monacrosporium ellopsoporum and Harposporium anguillu-lae. Of these, six fungi were tested for cultural characteristics except. H, anguillulae, extre-mely fastidious fungus in artificial media. Among 14 media tested in this experiment, Corn-meal Agar (CMA) and Oatmeal Agar (OMA) were the most suitable media for growing all six nematophagous fungi. Weakly saprophytic M. ellipsospoyum also grew vigoroualy on these two media. The radial growth, dry weight and sporulation of the fungi tested were quite diverse depending on the culture media. D. lobata revealed good growth and abundantly sporulated on Glucoes Peptone Agar (GPA). Although over-all growth of F, oxysporum was not satisfactory on Sucrose Nitrate Agar (SNA), the sporulation was best on this medium. Optimum conditious for mycelial growth and sporulation of nematophagous fungi ranged pH 5-8 and 20-$30^{\circ}C$ on SNA. D. lobata and F, oxysporum grew vigorously and most profusely sporulated on all media tested. They turned out an most promising biocontrol agents for their aggressive growth and sporulation over the ranges of temperature and pH ranges.

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Effects of treatment of Enterobacter ludwigii SJR3 on growth of tomato plant and its expression of stress-related genes under abiotic stresses (비생물적 스트레스 환경에서 Enterobacter ludwigii SJR3 처리 시 토마토의 생장과 스트레스-관련 유전자의 발현)

  • Kim, Na-Eun;Song, Hong-Gyu
    • Korean Journal of Microbiology
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    • v.52 no.2
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    • pp.148-156
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    • 2016
  • This study examined effects of Enterobacter ludwigii SJR3 showing a high 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, on growth of tomato plant and its expression of stress-related genes under drought and salt stress. SJR3 strain was inoculated at $10^6cell/g$ soil to 4-week grown tomato plants, and drought and salt stresses were treated. After additional incubation for 1 week, root length, stem length, fresh weight and dry weight of tomato plants treated with SJR3 increased by 37.8, 37.2, 96.8 and 146.6%, respectively compared to those of uninoculated plants in drought stress environment, and they increased by 19.2, 25.4, 19.5, and 105.8%, respectively in salt stress environment. Proline content in tomato leaves increased significantly under stress conditions as one of a protecting substance against stresses, but proline contents in tomato treated with SJR3 decreased by 62.1 and 54.1%, respectively. Relative expression of genes encoding ACC oxidase, ACO1 and ACO4, ethylene response factor genes ERF1 and ERF4, and some other stress-related genes were examined from tomato leaves. Compared to the non-stressed tomato, expressions of all stress-related genes increased significantly in the stressed tomato, but gene expressions in the inoculated tomato were similar to those of no-stressed control tomato. Therefore, E. ludwigii SJR3 may play an important role in mitigating drought and salt stress in plants, and can increase productivity of crops under various abiotic stresses.

Review on the Causes of Headache in Hyungsang Medicine (두통(頭痛)의 원인에 따른 형상의학적(形象醫學的) 고찰 -동의보감(東醫寶鑑) 두문(頭門)을 중심으로)

  • Lee, Dong-Min;Park, Seong-Ha;Lee, Yong-Tae
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.4
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    • pp.835-841
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    • 2007
  • The followings are concluded from the treatment of headache in Hyungsang medicine, focussed on 11 kinds of headaches in Donguibogam. Headache is classified into overall headache and migraine according to the affected region. The causes are divided into exogenous affection and internal injury; The former brings on headache due to Wind-Cold and headache due to Damp-Heat. The latter, reversal headache, headache due to adverse rising of phlegm, headache due to regurgitation of Gi, headache due to excessive Heat, headache due to excessive Damp, true headache, and alcoholic headache. Headache due to internal injury generally tends to show deficiency syndrome with external affection. Headache due to exogenous affections is common to those who have big head or white skin and to Bangkwang type, and woman. The primary causes are Wind-Cold and Wind-Heat. When the body is observed in the perspective of eight phases, Damp-Heat is to be produced in the front, and Dry-Damp, in the back. Headache due to Damp-Heat is susceptible to Yangmyeong meridian type whose body develops more in the front and to woman. In the perspective of the upper and the lower, Yangdu(that is, head) is related to Eumdu(that is, glans of penis). Headache is also caused by the problems of Eumdu ,such as deficiency of Essence in man, pathologic change of uterus in women, and San syndrome in lower abdomen. In the case of man, headache is frequently severe and difficult to treat because head is a root for man. Disharmony of Gi and blood between the right and the left brings out migraine and headache due to regurgitation of Gi. Migraine is usually accompanied by symptoms of exogenous affection and often afflicts Gi-type, Shin-type, Soyang meridian type, deer type, and Dam-type. Headache due to regurgitation of Gi is brought by Gi deficiency or blood deficiency so that symptoms of exogenous affection do not show. It is mainly common with old people and those who have sunken eyes induced by deficiency of stomach Gi. In the perspective of the upper, the middle, and the lower, the pathologic change of head, chest and abdomen also bring about headache. The pathologic cause of head is Wind-Heat ,which triggers overall headache, migraine, headache due to Wind-Cold, headache due to excessive Heat, The pathogen of chest is phlegm-Fire and brings out headache due to Damp-Heat and headache due to adverse rising of phlegm. The pathologic factor in abdomen is Cold-Damp and produces headache due to adverse rising of phlegm and headache due to excessive Damp. In case of women, headache is generally caused by phlegm-Fire and retention of undigested food.

THE VERIFICATION OF THE MTT ASSAY ON THE VIABILITY OF PERIODONTAL LIGAMENTAL CELLS IN RAT MOLARS THROUGH THE HISTOLOGIC EXAMINATION (쥐치아 치근면의 치주인대세포의 활성도를 평가하는 방법으로 MTT검색법의 적절성에 대한 조직학적인 검증)

  • Kim, Hyun-Ki;Kim, Eui-Seoung;Choi, In-Bok;Kim, Jin;Lee, Seung-Jong
    • Restorative Dentistry and Endodontics
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    • v.28 no.5
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    • pp.385-391
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    • 2003
  • The purpose of this study is to examine the viability of PDL cells in rat molars by using MTT assay and to verify the MTT assay through the histologic observation. Thirty of Sprague-Dawley white female rats of 4-weeks old with a body weight of about 100 grams were used. Groupings are as follows : Immediate Group : Positive control group(n=10)-after extraction immediately. Dried Group : Negative control group(n=10)-after drying for an hour under warm dry. $ViaSpan^{\circledR}$ Group : 1hour $ViaSpan^{\circledR}$ group(n=10)-after storing in $ViaSpan^{\circledR}{\;}at{\;}4^{\circ}C$ for 1hour. Ten teeth of each group were treated as same as above and replanted to the original socket of experimental animals. After two weeks of replantation. all the experimental animals were sacrificed. And after fixation, extracted maxillary jaw was dimineralized. After it was embedded in paraffin. serial section by $5\mu\textrm{m}$ was carried out and for construction of specimen, hematoxylin-eosin dye was used. The mean MTT measurement of immediate group(positive control) is 2.81 and the mean measurement of dried group(negative control) is 0.98 which is significantt differnt(P<0.05), The mean measurement of $ViaSpan^{\circledR}$ group is 2.65 and there is significant difference between dried group and $ViaSpan^{\circledR}$ group(P<0.05), However, there is no difference between immediate group and $ViaSpan^{\circledR}$ group. The average resorption points of immediate group is 3.03 points. In the dried group, average 6.44 points resorption and 2.68 points showed resorption in the $ViaSpan^{\circledR}$ group. Unlike with MTT assay, there was no significant difference between the immediate group and $ViaSpan^{\circledR}$ group. The usage of MTT assay as a viable cell marker may give us a better indication of the maintenance of periodontal ligament cell vitality.

Effects of Supplemention of Rehmannia radix on Performance and Physiological Status in Broiler Chicks (건지황 첨가가 육계의 성장과 생리적 변화에 미치는 영향)

  • 박성진;박희성;유성오
    • Korean Journal of Poultry Science
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    • v.25 no.4
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    • pp.195-202
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    • 1998
  • The present study was conducted to investigate the effects of dietary supplemental dry powder of Rehmannia radix (RR) root on the growth performance and physiological status of broiler chicks. The treatments consisted of corn-soybean meal control diet, and RR 0.5, 1, and 2% diets. A total of 160 Arbor Acre male broiler chicks were randomly allotted to 16 pens ; four pens per treatment and 10 birds per pen. The growth performance, feed intake and carcass weight did not show any differences among the treatment groups, but feed conversion was significantly lower (P< 0.05) in RR 1% group than that of the control group. The muscle color was lighter (P < 0.05) in RR lof group than those of the other treatment groups. The blood concentrations of protein, glutamic-oxaloacetic-transaminase (GOT) and glutamic- pyruvic-transaminase (GPT) were not different (P > 0.05) among the treatment groups, but the blood total cholesterol content was significantly decreased (P < 0.05) in RR 2% group than those the other treatment groups. The blood concentration of sugar was lower (P <0.05) in RR 2% group than those of the other treatment groups, and hemoglobin concentration was decreased (P <0.05) by addition RR. Crude fat content of thigh muscle was higher in RR 1% group than those of the other treatment groups, and bone hardness of broiler chicks fed RR was higher (P < 0.05) than that of the control group. The saturated fatty acid content of the thigh muscle tende to be high in control and RR 0.5% groups, and unsaturated fatty acid content tende to be high in RR 1 and 2% groups than those of the other treatment groups

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Effects of Some Soil Conditioners on Soil Physical Properties and Lettuce Growth (토양구조개선제(土壤構造改善劑) 처리(處理)가 토양물리성(土壤物理性)과 상추생육(生育)에 미치는 영향(影響))

  • Ryu, In-Soo;Han, Jeung-Lim;Jo, In-Sang
    • Korean Journal of Soil Science and Fertilizer
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    • v.28 no.3
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    • pp.249-255
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    • 1995
  • This study was conducted to investigate the effects of some soil conditioners, such as polyacrylamide(PAM), polyvinylalcohol(PVA) and Bitumen emulsion, on aggregate formation and stability, wetting angle, sorptivity and penetrability of the soil with different textures : sand, sandy loam, loam and clay loam. A pot experiment was carried out to find out the effect of treatment on the germination and growth of lettuce with three textures : sand, sandy loam and silt loam. Soil aggregates larger than 2mm in untreated soils at dry condition were naught in sand, 45% in sandy loam, 80% in loam and 90% in clay loam. Treatments of soil conditioners tended to increase the occurrence of soil aggregate larger than 2mm, which were 20~25% in sand, 55~75% sandy loam, but not affected greatly aggragate occurence in loam and clay loam. The aggregate instability was decreased by the soil conditioner treatment. The wetting angles of the soils were greatly changed by hydrophobic of Bitumen, but those were changed slightly by PVA and PAM application. The sorptivity and penetrability data indicated that the effects of different materials on these parameters differed depending upon soil texture. Application of PVA and PAM were no effect exceptive in sand. Application of Bitumen revealed that water movement was not showed in all soils. The germination rate, root weight and top plant weight of lettuce were increased in all soils by PAM treatment as compared to untreated soils. Particularly the greater effect was occurred in sand soil than in sandy loam and silt loam. PAM increased greatly the moisture content and air phase of soils.

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Physiological and Anatomical Studies of Quinclorac Action (Quinclorac의 작용성(作用性)에 대한 연구(硏究))

  • Hong, S.Y.;Lee, I.J.;Kim, K.U.;Shin, D.H.;Lee, C.N.
    • Korean Journal of Weed Science
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    • v.13 no.1
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    • pp.62-70
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    • 1993
  • There was intraspecific variation in Echinochloa crus-galli var, crus-galli in response to quinclorac, showing that plan height and dry weight of a locally collected barnyardgrass(Chinjupi) from Chinju were 90.5 and 37.8% of the untreated control, while those of a locally collected one(Iripi) from Iri showed 19.1 and 14.4%, respectively. The normal distribution curve was obtained from frequency distribution of 89 rice cultivars as affected by the application rates of quinclorac at 30, 300, and 3,000g ai/ha. Protein patterns(SDS-PAGE) of two barnyardgrasses belonging to E, crus-galli var. crus-galli such as Iripi and Chinjupi were not affected by the quinclorac application, indicating that inhibition of enzyme and/or protein biosynthesis seems to be not the primary action target of quinclorac. Electronmicroscopic observation on the injured leaf of Iripi which is considered as a susceptible one showed prominent membrane disruption. Chuchungbyeo(rice variety) resulted in a greater inhibition of tomato growth than those from Chinjupi or Iripi, indicating a great amount of quinclorac discharged from rice root, Chinjupi which is relatively tolerant to quinclorac than Iripi, discharged more quinclorac causing a greater inhibition of tomato growth.

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