• Journal of Microbiology and Biotechnology
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• v.9 no.2
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• pp.179-183
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• 1999

A recombinant form of hirudin, a potent thrombin-specific inhibitor derived from the bloodsucking leech, was expressed as a secretory product in Saccharomyces cerevisiae under the control of GALl0 promoter and the mating factor $\alpha$pre-pro leader sequence. In an attempt to produce recombinant hirudin (r-Hir) of therapeutic purity in large quantities, the fed-batch fermentation was carried out by using this recombinant yeast, and subsequently downstream processing was developed with the preparative-scale column chromatography systems. About 234 mg/l of biologically active r-Hir was produced as a secretory product by the fed-batch fermentation strategy developed for an efficient downstream processing. Using a two-step chromatography process (an anion exchange chromatography followed by the reverse phase HPLC), the r-Hir was purified to>98% with an overall recovery yield of 84%. According to the N-terminal amino acid sequencing, the purified r-Hir was found to have the predicted N-terminal amino acid sequence. The biological activity of the purified r-Hir to inhibit thrombin was also identical to that of the commercial hirudin.

• Journal of Information Processing Systems
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• v.2 no.2
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• pp.130-134
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• 2006

In the distributed replicating server model, the provision of replicated services will improve the performance of the providing service and efficiency for clients. Efficiently composing the server selection algorithm decreases the retrieval time for replicated data. In this paper, we define the system model that selects and connects the replicated server that provides an optimal service using the server-side downstream measurement and propose a server selection algorithm.

• Journal of Information Processing Systems
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• v.17 no.5
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• pp.918-932
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• 2021

Evaluation of the collaboration of the upstream and downstream enterprises in the manufacturing supply chain is important to improve their synergistic effect. From the supply chain perspective, this study establishes the evaluation model of the manufacturing enterprise collaboration on the basis of fuzzy entropy according to synergistic theory. Downstream enterprises carry out coordinated capital, business, and information flows as subsystems and research enterprises as composite systems. From the three subsystems, the collaboration evaluation index is selected as the order parameter. The compound fuzzy matter-element matrix is established by using its improved algorithm. Subordinate membership and standard deviation fuzzy matter-element matrixes are constructed. Index weight is determined using the entropy weight method. The closeness of each matter element is then calculated. Through a representative of the home appliance industry, namely, Gree Electric Appliances Inc. of Zhuhai, empirical analysis of data in 2011-2017 from the company and its upstream and downstream enterprise collaboration shows a good trend, but the coordinated development has not reached stability. Gree Electric Appliances Inc. of Zhuhai need to strengthen the synergy with upstream and downstream enterprises in terms of cash, business, and information flows to enhance competitiveness. Experimental results show that this method can provide precise suggestions for enterprises, improve the degree of collaboration, and accelerate the development and upgrading of the manufacturing industry.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.1
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• pp.85-90
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• 2005

A downstream process was developed for the production of yeast extract from brewer's yeast cells. Various downstream processing conditions including clarification, debittering, and the Maillard reaction were considered in the development of the process. This simple and economic clarification process used flocculating agents, specifically calcium chloride ($1\%$). After the clarification step, a Maillard reaction is initiated as a flavor-enhancing step. By investigating the effects of several operation parameters, including the type of sugar added, sugar dosage, glycine addition, and temperature, on the degree of browning (DB), giucose addition and reaction temperature were found to have significant effects on DB. A synthetic adsorption resin (HP20) was used for the debittering process, which induced a compositional change of the hydrophobic amino acids in the yeast hydrolysate, thereby reducing the bitter taste. The overall dry matter yield and protein yield for the entire process, including the downstream process proposed for the production of brewer's yeast extract were 50 and $50\%$, respectively.

• Proceedings of the Korea Information Processing Society Conference
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• 2016.04a
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• pp.51-54
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• 2016

NTB는 transparent bridge와 공통적으로 독립적인 PCI bus(PCI 또는 PCI Express bus)에 대해서 데이터 전송 경로(path)를 제공한다는 점에서 기능적으로 유사하다. 그러나, NTB와 transparent bridge 간의 가장 큰 차이점은 NTB가 사용될 경우에 bridge의 하향부분(downstream side)에 위치한 장치들은 상향부분(upstream side)에서는 보이지 않는다는 점이다. 이는 bridge의 하향부분(downstream side)에 위치한 인텔리전트(intelligent)한 제어기(예를들면 CPU를 포함하는 컴퓨터)가 자신의 downstream side에 위치하는 서브시스템 내 각종 장치들을 독립적으로 관리할 수 있다는 점이다. NTB는 또한 첫 번째 호스트(primary host)의 PCI bus로 구성된 서브시스템(subsystem) 계층구조(hierarchy)에 두 번째 호스트(secondary host)를 연결하는 데 사용될 수 있다. 이는 두 시스템간 통신을 가능하도록 하는 반면, 두 시스템을 서로 격리시키는 효과도 발생한다. 즉, NTB는 일반적으로 도어벨(doorbell)을 통해서 bridge의 다른 편에 위치한 장치에 대해서 인터럽트를 보낼 수 있으며, 또한, scratchpad 레지스터를 보유하고 있어 bridge의 양측에서 데이터를 상호 공유함으로써 interprocessor communication 할 수 있다.

• Journal of the Institute of Convergence Signal Processing
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• v.12 no.2
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• pp.102-106
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• 2011

The transfer capability of HFC (Hybrid Fiber Coax) network is superior to xDSL. HFC network, however, is not suitable for transferring high quality video due to cable model interfaces. For the services of high quality IPIV or VOD, the extra exclusive downstream transfer system is required without upgrading pre-equipped cable modem and service capability. This paper is aimed to develop the extra exclusive downstream transfer system not changing existing cable modem system but providing same quality of services. This system is composed of the extra exclusive downstream IP-cable sender and modem. This sender and modem have 30 Mbps transfer capability and HDTV stream can be served in the Cable TV network using 21 Mbps HDTV transport stream.

• Transactions of the Korean Society of Automotive Engineers
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• v.6 no.4
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• pp.219-227
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• 1998

This paper describes the fuel spray characteristics of gasoline port injectors such as the breakup procedures of liquid fuel, breakup and extinction behaviors of fuel spray at nozzle tip, time history of SMD and velocity distribution of fuel spray in the direction of fuel stream. Pintle-type gasoline fuel injector was used to analyze mentioned spray characteristics. In order to visualize the fuel spray behaviors and to measure the droplet mean diameter and velocities of spray droplets, the Schlieren method, digital image processing and auto-correlation PIV were applied in this study. In addition, the spray characteristics according to the variation of time were considered. The results of fuel spray show that the liquid sheet breakup starts at 10mm downstream actively. The flying time is approximately 4msec between 50mm and 80mm down the nozzle tip. Also, SMD of fuel spray, the number of droplets and fuel velocity distribution at each point of downstream are discussed.

• Proceedings of the Korea Information Processing Society Conference
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• 2002.11b
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• pp.1499-1502
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• 2002

DiffServ 망에서 완전한 QoS를 망 사용자에게 제공하기 위해서는 downstream 트래픽 뿐만 아니라, upstream 트래픽에 marking, policing 을 적용해 주어야 한다. 본 논문에서는 downstream 트래픽에만 QoS를 적용하는 단방향 트래픽 제어기법과, downstream및 upstream 트래픽에 QoS를 적용하는 양방향 QoS 서비스 기법을 비교하기 위하여, cisco 라우터를 이용하여 QoS 테스트베드를 구성하고, 성능을 측정 및 분석하였다.

• Journal of Information Processing Systems
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• v.2 no.2
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• pp.76-81
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• 2006

A PDA is used mainly for downloading data from a stationary server such as a desktop PC in an infrastructure network based on wireless LAN. Thus, the overall performance depends heavily on the performance of such downloading with PDA. Unfortunately, for a PDA the time taken to receive data from a PC is longer than the time taken to send it by 53%. Thus, we measured and analyzed all possible factors that could cause the receiving time of a PDA to be delayed with a test bed system. There are crucial factors: the TCP window size, file access time of a PDA, and the inter-packet delay that affects the receiving time of a PDA. The window size of a PDA during the downstream is reduced dramatically to 686 bytes from 32,581 bytes. In addition, because flash memory is embedded into a PDA, writing data into the flash memory takes twice as long as reading the data from it. To alleviate these, we propose three distinct remedies: First, in order to keep the window size at a sender constant, both the size of a socket send buffer for a desktop PC and the size of a socket receive buffer for a PDA should be increased. Second, to shorten its internal file access time, the size of an application buffer implemented in an application should be doubled. Finally, the inter-packet delay of a PDA and a desktop PC at the application layer should be adjusted asymmetrically to lower the traffic bottleneck between these heterogeneous terminals.

• The KIPS Transactions:PartD
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• v.10D no.1
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• pp.161-166
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• 2003

Information lead time is defined as the time spent by processing orders from some buyers, whereas order lead time is defined as producing and supplying the products. The information lead time significantly serve to magnify the increase in variability due to demand forecasting. This paper models a decentralized supply chain composed of cascade type which has four type phases (or divisions) such as retailer, wholesaler, distributor, and factory. Each phases is managed by different centers individually with their own local inventory information. We investigate whether each phase's Information lead time affects companies networked a value chain. In particular, on several experiments performed with a programmed simulation (like a MIT beer game), we study the following question ; Can information lead times do better than material lead times in cost-benefit perspective\ulcorner Can more much Information lead times in downstream reasonably do worser than in upstream when playing the simulation\ulcorner In the conclusion, we show the importance of information lead time on a SC and, besides, guarantee that improvement of information lead time in upstream do more effective than one in downstream in cost-benefit perspective.