• Journal of Gifted/Talented Education
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• v.13 no.4
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• pp.119-140
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• 2003

The purpose of this study is to examine the dispute over creativity domain at schools in Korea. In other words, this study is to examine the creativity domain among all subjects, a group of high level performance, and a group of low level performance in creativity. Based on ability-differentiation hypothesis, correlation among 4 domains, correlation between divergent thinking variables of domain-generality and 4 domains of domain-speciality, and multiple linear regression have been computed. The results are opposed to the hypothesis. The group of low level performance is related to domain-speciality. The group of high level performance, however, has relationships neither domain-generality nor domain-speciality. Instead of differentiation between domain-generality and domain-speciality, the results support the domain-complementarity.

• Communications of the Korean Mathematical Society
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• v.24 no.3
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• pp.425-432
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• 2009

This paper introduces a local non-positivity of two set-valued mappings (F,Q) and considers the existences and properties of solutions for set-valued mixed vector FQ-implicit variational inequality problems and set-valued mixed vector FQ-complementarity problems in the neighborhood of a point belonging to an underlined domain K of the set-valued mappings, where the neighborhood is contained in K. This paper generalizes and extends many results in [1, 3-7].

• Transactions of the Korean Society of Mechanical Engineers
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• v.18 no.8
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• pp.2026-2038
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• 1994

A numerical scheme is developed for the analysis of incipient sliding contact with orthotropic friction condition subjected to tangential load and twisting moment. The inherent nonlinearity in the orthotropic friction law has been treated by a polyhedral friction law. Then, a three-dimensional linear complementarity problem(LCP) formulation in an incremental form is obtained, and the existence of a solution is investigated. A Lemke's complementary pivoting algorithm is used for solving the LCP. The scheme is illustrated by spherical contact problems, and the effects of eccentricity of elliptical friction domain on the traction and stick region are discussed.

• Ocean and Polar Research
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• v.33 no.2
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• pp.159-169
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• 2011

Antifreeze proteins (AFPs) have ice binding affinity, depress freezing temperature and inhibit ice recystallization which protect cellular membranes in polar organisms. Recent structural studies of antifreeze proteins have significantly expanded our understanding of the structure-function relationship and ice crystal growth inhibition. Although AFPs (Type I-IV AFP from fish, insect AFP and Plant AFP) have completely different fold and no sequence homology, they share a common feature of their surface area for ice binding property. The conserved ice-binding sites are relatively flat and hydrophobic. For example, Type I AFP has an amphipathic, single ${\alpha}$-helix and has regularly spaced Thr-Ala residues which make direct interaction with oxygen atoms of ice crystals. Unlike Type I AFP, Type II and III AFP are compact globular proteins that contain a flat ice-binding patch on the surface. Type II and Type III AFP show a remarkable structural similarity with the sugar binding lectin protein and C-terminal domain of sialic acid synthase, respectively. Type IV is assumed to form a four-helix bundle which has sequence similarity with apolipoprotein. The results of our modeling suggest an ice-binding induced structural change of Type IV AFP. Insect AFP has ${\beta}$-helical structure with a regular array of Thr-X-Thr motif. Threonine residues of each Thr-X-Thr motif fit well into the ice crystal lattice and provide a good surface-surface complementarity. This review focuses on the structural characteristics and details of the ice-binding mechanism of antifreeze proteins.

• Journal of Educational Research in Mathematics
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• v.17 no.3
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• pp.253-270
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• 2007

by A.C. Clairaut was written based on the historico-genetic principle such as his . In this paper, by analyzing his we can induce six principles that Clairaut adopted to teach algebra: necessity and curiosity as a motive of studying algebra, harmony of discovery and proof, complementarity of generalization and specialization, connection of knowledge to be learned with already known facts, semantic approaches to procedural knowledge of mathematics, reversible approach. These can be considered as strategies for teaching algebra accorded with beginner's mind. Some of them correspond with characteristics of , but the others are unique in the domain of algebra. And by comparing Clairaut's approaches with school algebra, we discuss about some mathematical subjects: setting equations in relation to problem situations, operations and signs of letters, rule of signs in multiplication, solving quadratic equations, and general relationship between roots and coefficients of equations.

• Molecules and Cells
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• v.39 no.3
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• pp.217-228
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• 2016

To generate a biobetter that has improved therapeutic activity, we constructed scFv libraries via random mutagenesis of several residues of CDR-H3 and -L3 of hu4D5. The scFv clones were isolated from the phage display libraries by stringent panning, and their antiproliferative activity against HER2-positive cancer cells was evaluated as a primary selection criterion. Consequently, we selected AH06 as a biobetter antibody that had a 7.2-fold increase in anti-proliferative activity ($IC_{50}$: 0.81 nM) against the gastric cancer cell line NCI-N87 and a 7.4-fold increase in binding affinity ($K_D$: 60 pM) to HER2 compared to hu4D5. The binding energy calculation and molecular modeling suggest that the substitution of residues of CDR-H3 to W98, F100c, A101 and L102 could stabilize binding of the antibody to HER2 and there could be direct hydrophobic interactions between the aromatic ring of W98 and the aliphatic group of I613 within HER2 domain IV as well as the heavy and light chain hydrophobic interactions by residues F100c, A101 and L102 of CDR-H3. Therefore, we speculate that two such interactions were exerted by the residues W98 and F100c. A101 and L102 may have a synergistic effect on the increase in the binding affinity to HER2. AH06 specifically binds to domain IV of HER2, and it decreased the phosphorylation level of HER2 and AKT. Above all, it highly increased the overall level of p27 compared to hu4D5 in the gastric cancer cell line NCIN82, suggesting that AH06 could potentially be a more efficient therapeutic agent than hu4D5.

• Molecules and Cells
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• v.40 no.9
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• pp.655-666
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• 2017

We constructed a large $na{\ddot{i}}ve$ human Fab library ($3{\times}10^{10}$ colonies) from the lymphocytes of 809 human donors, assessed available diversities of the heavy-chain variable (VH) and ${\kappa}$ light-chain variable (VK) domain repertoires, and validated the library by selecting Fabs against 10 therapeutically relevant antigens by phage display. We obtained a database of unique 7,373 VH and 41,804 VK sequences by 454 pyrosequencing, and analyzed the repertoires. The distribution of VH and VK subfamilies and germline genes in our antibody repertoires slightly differed from those in earlier published natural antibody libraries. The frequency of somatic hypermutations (SHMs) in heavy-chain complementarity determining region (HCDR)1 and HCDR2 are higher compared with the natural IgM repertoire. Analysis of position-specific SHMs in CDRs indicates that asparagine, threonine, arginine, aspartate and phenylalanine are the most frequent non-germline residues on the antibody-antigen interface and are converted mostly from the germline residues, which are highly represented in germline SHM hotspots. The amino acid composition and length-dependent changes in amino acid frequencies of HCDR3 are similar to those in previous reports, except that frequencies of aspartate and phenylalanine are a little higher in our repertoire. Taken together, the results show that this antibody library shares common features of natural antibody repertoires and also has unique features. The antibody library will be useful in the generation of human antibodies against diverse antigens, and the information about the diversity of natural antibody repertoires will be valuable in the future design of synthetic human antibody libraries with high functional diversity.