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1D Proton NMR Spectroscopic Determination of Ethanol and Ethyl Glucuronide in Human Urine

  • Kim, Siwon;Lee, Minji;Yoon, Dahye;Lee, Dong-Kye;Choi, Hye-Jin;Kim, Suhkmann
    • Bulletin of the Korean Chemical Society
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    • v.34 no.8
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    • pp.2413-2418
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    • 2013
  • Forensic and legal medicine require reliable data to indicate excessive alcohol consumption. Ethanol is oxidatively metabolized to acetate by alcohol dehydrogenase and non-oxidatively metabolized to ethyl glucuronide (EtG), ethyl sulfate (EtS), phosphatidylethanol, or fatty acid ethyl esters (FAEE). Oxidative metabolism is too rapid to provide biomarkers for the detection of ethanol ingestion. However, the non-oxidative metabolite EtG is a useful biomarker because it is stable, non-volatile, water soluble, highly sensitive, and is detected in body fluid, hair, and tissues. EtG analysis methods such as mass spectroscopy, chromatography, or enzyme-linked immunosorbent assay techniques are currently in use. We suggest that nuclear magnetic resonance (NMR) spectroscopy could be used to monitor ethanol intake. As with current conventional methods, NMR spectroscopy doesn't require complicated pretreatments or sample separation. This method has the advantages of short acquisition time, simple sample preparation, reproducibility, and accuracy. In addition, all proton-containing compounds can be detected. In this study, we performed $^1H$ NMR analyses of urine to monitor the ethanol and EtG. Urinary samples were collected over time from 5 male volunteers. We confirmed that ethanol and EtG signals could be detected with NMR spectroscopy. Ethanol signals increased immediately upon alcohol intake, but decreased sharply over time. In contrast, EtG signal increased and reached a maximum about 9 h later, after which the EtG signal decreased gradually and remained detectable after 20-25 h. Based on these results, we suggest that $^1H$ NMR spectroscopy may be used to identify ethanol non-oxidative metabolites without the need for sample pretreatment.

Analysis of Effect of Ditch Restoration on Soil Loss Reduction in Highland Agricultural Fields (고랭지밭의 구거복원에 따른 토양유실저감 효과분석)

  • Sung, Yunsoo;Kim, Dong Jin;Lee, Suin;Ryu, Jichul;Kim, Jonggun;Lim, Kyoung Jae;Kim, Ki Sung
    • Journal of Korean Society on Water Environment
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    • v.36 no.5
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    • pp.385-391
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    • 2020
  • Soil loss is a serious problem frequently caused by local torrential rainfalls due to climate change. In particular, soil loss is occurring in agricultural areas rather than urban areas, and many pollutants are introduced into rivers, causing environmental problems. To reduce soil loss, the Ministry of Environment has designated and managed non-point source management areas. The Jaun-district in Hongcheon-gun, which was designed as a non-point pollution source management area in Gangwon-do, is located in the upper stream of Soyang Lake. Most of the agricultural fields are composed of highland agriculture fields. The highland agricultural fields in the Jaun-district are also composed of large-scale farming areas, and the ditches located near the agricultural fields have been illegally used for farmland. Therefore, the local government in Hongcheon-gun is conducting a project to restore the ditches occupied by agricultural fields. However, an analysis of the amount of soil loss that can be reduced by the restoration of the ditches has not been conducted yet. Thus, the purpose of this study was to analyze the effect of reducing the soil loss from the restoration of the ditches used as agricultural fields in the Jaun-district. The SATEEC L Module was used to analyze the reduction in soil loss by ditch restoration. The SATEEC L Module was constructed to estimate the LS factor using Moore and Burch's method after calculating the slope length using the digital elevation model and the maximum allowable slope length. The LS factor and the USLE formula were used to estimate the amount of soil loss that could be reduced by ditch restoration. The analysis showed that the ditch restoration could reduce about 16.6% of the soil loss in the Jaun-district. The results of this study will contribute to the study of methods to reduce soil loss in non-point pollution management areas.

Conceptual Design Strategy of Renewable Energy Application for Building Certification and Mandatory System (신재생에너지설비 건물설치 의무화 및 인증제를 고려한 용량설계 방안)

  • Lee, Kyoung-Ho;Lee, Dong-Won;Kown, Hyeok-Min;Lee, Chang-Jun
    • 한국태양에너지학회:학술대회논문집
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    • 2011.11a
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    • pp.99-104
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    • 2011
  • This paper describes a design strategy at conceptual design stage using RETScreen software tool for building application of renewable energy resources. Currently, government and public buildings are required to adopt renewable energy systems with a minimum requirement for the amount of renewable energy supply. Meanwhile, there is a certificate program for private office buildings to enhance propagation of renewable energy systems. When considering application of renewable energy systems to a building, it is worthwhile developing a method to determine optimal design sizes of renewable energy systems. In the paper, a design strategy is introduced with a couple of case studies to determine optimal capacities of each renewable energy system in a building and suggest to use the method to evaluate the system for the building certificate program and the mandatory renewable target program. Objective functions considered in the study are initial system cost and reduction of CO2 emissions from the system. In the optimization study, it is assumed that solar thermal collectors are installed to satisfy solar fraction of 60%. Other renewable energy systems such as ground-source heat pump, solar PV and non-renewable systems such as electric chiller and gas-fired boiler are sized using an optimal sizing method with RETScreen suggested the authors previously.

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Analysis of the Eco-experience Program of the Restoration Rivers in Seoul (서울시 복원하천의 생태체험 프로그램 분석)

  • Kim, Dong-Chan;Kim, Shin-Won;You, Kyung-Min
    • KIEAE Journal
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    • v.10 no.6
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    • pp.47-54
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    • 2010
  • The significant aim of this research is to suggest the applicable data for the future urban ecological restoration of rivers' activity and the direction of the future eco-experience program found by analyzing the state and progress of the restoration of the river ecological experience program. For this purpose, we researched the eco-experience program's outline and details of its operation and method from six rivers among all of the rivers going through restoration. We also carried out telephone interviews and in-depth interviews with the office staff that takes care about each river at the district office to investigate the specific details not revealed by our analysis, the number of operations, and the number of participants. We compared the number of operations to the number of participants and the frequency of participation of each river in a year. As a result, they have revealed that the participants of the eco-experience program ranged in variety including local students, families, and local citizens as a whole. Also the intimate observation and experience with nature gave the participants a better understanding of the ecosystem. In addition, it is revealed that the cheonggye river goes through its own standard of strict inspections and management daily. It is quite easy to notice that the cheonggye river shows a higher level of participation and operating activity than other rivers.

Condensation Heat Transfer Correlation for Smooth Tubes in Annular Flow Regime

  • Han Dong-Hyouck;Moon C.;Park C.;Lee Kyu-Jung
    • Journal of Mechanical Science and Technology
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    • v.20 no.8
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    • pp.1275-1283
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    • 2006
  • Condensation heat transfer coefficients in a 7.92 mm inside diameter copper smooth tube were obtained experimentally for R22, R134a, and R410A. Working conditions were in the range of $30-40^{\circ}C$ condensation temperature, $95-410 kg/m^2s$ mass flux, and 0.15-0.85 vapor quality. The experimental data were compared with the eight existing correlations for an annular flow regime. Based on the heat-momentum analogy, a condensation heat transfer coefficients correlation for the annular flow regime was developed. The Breber et al. flow regime map was used to discern flow pattern and the Muller-Steinhagen & Heck pressure drop correlation was used for the term of the proposed correlation. The proposed correlation provided the best predicted performance compared to the eight existing correlations and its root mean square deviation was less than 8.7%.

Development and Application of Water Balance Network Model in Agricultural Watershed (농업용수 유역 물수지 분석 모델 개발 및 적용)

  • Yoon, Dong-Hyun;Nam, Won-Ho;Koh, Bo-Sung;Kim, Kyung-Mo;Jo, Young-Jun;Park, Jin-Hyeon
    • Journal of The Korean Society of Agricultural Engineers
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    • v.66 no.3
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    • pp.39-51
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    • 2024
  • To effectively implement the integrated water management policy outlined in the National Water Management Act, it is essential to analyze agricultural water supply and demand at both basin and water district levels. Currently, agricultural water is primarily distributed through open canal systems and controlled by floodgates, yet the utilization-to-supply ratio remains at a mere 48%. In the case of agricultural water, when analyzing water balance through existing national basin water resource models (K-WEAP, K-MODISM), distortion of supply and regression occurs due to calculation of regression rate based on the concept of net water consumption. In addition, by simplifying the complex and diverse agricultural water supply system within the basin into a single virtual reservoir, it is difficult to analyze the surplus or shortage of agricultural water for each field within the basin. There are limitations in reflecting the characteristics and actual sites of rural water areas, such as inconsistencies with river and reservoir supply priority sites. This study focuses on the development of a model aimed at improving the deficiencies of current water balance analysis methods. The developed model aims to provide standardized water balance analysis nationwide, with initial application to the Anseo standard watershed. Utilizing data from 32 facilities within the standard watershed, the study conducted water balance analysis through watershed linkage, highlighting differences and improvements compared to existing methods.

A Study on the Improvement of the Management System of Rockfall Risk Area Using the Rockfall Analysis Program (낙석 해석 프로그램을 이용한 낙석위험지역 관리체계 개선 방안에 대한 연구)

  • Bae Dong Kang;Jae Chae Jeong;Chang Deok Jang;Kye Won Jun
    • Journal of Korean Society of Disaster and Security
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    • v.15 no.4
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    • pp.79-86
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    • 2022
  • The National Park Service is making efforts to create a safe environment by installing rockfall prevention facilities (rockfall prevention nets, fences, and Piam tunnels) in areas at risk of falling rocks. However, the occurrence of falling rocks is increasing every year due to torrential rains caused by climate change, abnormal temperatures in winter, and aging of the ground, and the need to improve the existing rock risk area management plan has emerged. In this study, a pilot area at risk of falling rocks was selected for the Hwanggol district of Chiaksan National Park among Korean national parks, and rockfall analysis was performed using the Rockfall program, and monitoring was conducted by applying a countermeasure method combined with the measurement system to the pilot area. Through this, a rockfall management plan was proposed for continuous management and monitoring of rockfall.

Construction of Environmental-friendly Infrastructure in Saemangeum Reclaimed Land (새만금 간척지 토양특성과 친환경 활용 방안)

  • Seo, Dong-Uk;Jeon, Geon-Yeong;Kim, Hyun-Tae;Song, Jae-Do
    • Proceedings of the Korean Geotechical Society Conference
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    • 2010.09a
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    • pp.40-48
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    • 2010
  • Saemangeum reclaimed area is needed to construct much green zone to make high-quality multi-functional land such as tide embankment, lake dike, industrial complex, environmental spaces, etc. However, growth of plants is somewhat difficult because a salinity of Saemangeum soil is very high and a soil fertility, water content of soil are low. Therefore, it is essential to initial desalination of soil and continuous management for planting base. It is recommended that a group of grassland to raise the efficiency of covering should be made in the first stage and a forest by improvement of vegetation should be made in the mid and long term stage. It is recommended that the construction of vegetation base should be made with a regular thickness of soil of good quality in multi-functional area such as a shrub and wood. In case of construction of a windbreak forest, it is necessary to make a wood base of suitable depth using soil brought from another place or filling of soil. Also, it is necessary to keep a maintenance of woods in early stage. Saemangeum reclaimed land will be brand-named worldwide tourist attractions due to construction of much green zone having high quality multi-functional facilities.

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Evaluation of the Heating and Cooling Systems in School Buildings (학교건물 냉난방설비시스템의 실태조사)

  • AHN, Chul-Lin;KIM, Dong-Gyue;KUM, Jong-Soo;PARK, Jong-Un;PARK, Hee-Ouk;CHUNG, Yong-Hyun
    • Journal of Fisheries and Marine Sciences Education
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    • v.17 no.1
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    • pp.18-25
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    • 2005
  • We have researched 10,811 schools to evaluate the characteristics of school buildings and the conditions of heating and cooling systems at the elementary, middle and high school levels. Air conditioning systems in school buildings are related to school scale, and 35% of the researched schools have less than 10 air conditioned classrooms among all of the schools in Korea. The LOADSYS is used to grasp the characteristics of school buildings heating and cooling load. From the results of this work, the heating load differs by nearly 24% between Seoul and Busan, but other than that there are not so many serious regional differences. Almost 85.4% of the classrooms are equipped with heating facilities and 6.9% of them are equipped with heating and cooling facilities. As a result, it is necessary to make improvements in 31.8% of the classrooms using only heaters and 14.6% of the classrooms not equipped with HVAC. The survey shows that there is a wide gap in the heating equipment status of the classrooms according to the region and school district.