• Journal of Life Science
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• v.19 no.2
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• pp.169-173
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• 2009

To assist in selection schemes we estimate the genetic diversity of the horse breeds. Genetic diversity at 13 microsatellite loci was compared in six horse breeds : Jeju Native Horse, American Quarter, Jeju Racing Horse, Mongolian Horse, Japanese Horse and Thoroughbred. All of the equine microsatellite used in this study were amplified and were polymorphic. The expected total heterozygosity over all the populations varied between 0.669 and 0.869 and the expected heterozygosity within population range from 0.569 to 0.219 in this study. The low coefficient of gene differentiation value showed that only 0.118 of the diversity was between horses breeds. The constructed dendrogram from the genetic distance matrix showed little differentiation between horse breeds using DISPAN program. The genetic distance using 13 microsatellites ranged between 0.137 and 0.414 for the six horse breeds. These results confirm the potential use of equine microsatellite loci as a tool for genetic studies in horse populations. The genetic diversity of the six horse breeds to each other closed to their geographical distribution. Suggesting that the loci would be suitable for horse breeds parentage testing. Therefore, Microsatellite marker seems to be very useful for clarifying the evolutionary relationships of closely related populations.

• Resources Recycling
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• v.23 no.6
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• pp.3-11
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• 2014

Bottom ash from coal fired power plants is not widely used due to a broad range of particle sizes and a high carbon content for producing geopolymers. The effect of mechanical activation on compressive strength of bottom ash- based geopolymers was examined by rod and planetary-ball milling to encourage full-fledged recycling of bottom ash, the main component of pond ash. The amount of amorphous component in the milled ash samples did not change significantly after the mechanical activation. It is presumably because needle-shaped mullite crystals, which is a major crystalline phase and grown in a glassy matrix, possess high strength and toughness, and therefore, they could endure external shocks and remain almost intact. Milling operation, however, decreased the particle size and improved the homogeneity of ash, thereby leading to increase reactivity of milled ash with alkali activators. Rod milling produced a relatively narrow particle size distribution of the milled ash particles; however, it was less effective in reducing the particle size. Nevertheless, it was interesting to observe that rod milling had equal effect on improving the compressive strength of geopolymers up to about 37%, as that of planetary ball milling. Rod milling is believed to be suitable process for enhancing the reactivity of bottom ash for large-scale recycling of bottom ash and producing geopolymers.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.4 no.1
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• pp.59-64
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• 2006

Ion exchange resin particles should not be found in steam generator(S/G) sludge. The suspicious spherical resin particles observed in S/G sludge sample were characterized for particle size distribution under optical microscope using the micro-technique, for element analysis by the electron probe micro analysis (EPMA), and for molecular identification by the IR spectroscopy. The particle sizes are distributed from 1 to $200{\mu}m$ for the sludge, while 40 to $500{\mu}m$ for the spherical resin particles. The results of the elemental analysis showed different major impurities: Si, Al, Mn, Cr, Ni, Zn and Ti for the sludge particles, while Si, Cu, Zn for the spherical resin particles. However, both particles contain Fe as a matrix of magnetite $(Fe_3O_4)$. IR spectrum of the spherical particles was not quite similar to the IR spectrum of ion exchange resins used in S/G system. These results indicate that the spherical particles are not related to ion exchange resin particles and may be formed by the process of the sludge formation.

• Polymer(Korea)
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• v.34 no.1
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• pp.1-7
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• 2010

To enhance the transport properties of gas separation membrane, we prepared 6FDA-6FpDA based polyimide membrane with PMMA-graft-silica nanoparticles. The silica was grafted PMMA which is miscible with 6FDA-based polyimide after surface treatment by 3-methacryloxypropyltrimethoxysilane ($\gamma$-MPS). The untreated silica/6FDA-6FpDA membrane showed greater permeability and less selectivity than PMMA-g-silica/6FDA-6FpDA due to its low dispersion. The transport properties of PMMA-g-silica/6FDA-GFpDA membrane were measured as a function of filler concentration. These membranes were evaluated using pure gases (He, $O_2$, $N_2$, $CO_2$). The increase in permeation was attributed to changes in the free volume distribution until 1 wt%. After 1 wt%, the permeability was decreased by excess silica which decreased effective area in polymer matrix. The selectivity was decreased with increasing permeability on the whole. However, the selectivity of $CO_2$ showed more enhance value.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.73-73
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• 2018

Bacterial leaf blight(BLB), caused by X. oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice due to its high epidemic potential. Understanding BLB resistance at a genetic level is important to further improve the rice breeding that provides one of the best approaches to control BLB disease. In the present investigation, a collection of 192 accessions was used in the genome-wide association study (GWAS) for BLB resistance loci against four Korean races of Xoo that were represented by the prevailing BLB isolates under Xoo differential system. A total of 192 accessions of rice germplasm were selected on the basis of the bioassay using four isolated races of Xoo such as K1, K2, K3 and K3a. The selected accessions was used to prepare 384-plex genotyping by sequencing (GBS) libraries and Illumina HiSeq 2000 paired- end read was used for GBS sequencing. GWAS was conducted using T ASSEL 5.0. The T ASSEL program uses a mixed linear model (MLM). T he results of the bioassay using a selected set of 192 accessions showed that a large number of accessions (93.75%) were resistant to K1 race, while the least number of accessions (34.37%) resisted K3a race. For races K2 and K3, the resistant germplasm proportion remained between 66.67 to 70.83%. T he genotypic data produced SNP matrix for a total of 293,379 SNPs. After imputation the missing data was removed, which exhibited 34,724 SNPs for association analysis. GWAS results showed strong signals of association at a threshold of [-log10(P-value)] more than5 (K1 and K2) and more than4 (K3 and K3a) for nine of the 39 SNPs, which are plausible candidate loci of resistance genes. T hese SNP loci were positioned on rice chromosome 2, 9, and 11 for K1 and K2 races, whereas on chromosome 4, 6, 11, and 12 for K3 and K3a races. The significant loci detected have also been illustrated, NBS-LRR type disease resistance protein, SNARE domain containing protein, Histone deacetylase 19, NADP-dependent oxidoreductase, and other expressed and unknown proteins. Our results provide a better understanding of the distribution of genetic variation of BLB resistance to Korean pathogen races and breeding of resistant rice.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.1
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• pp.201-208
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• 2013

Objective: To investigate changes in the invasive capacity of gastric cancer cells in vitro after expression inhibition of T lymphoma invasion and metastasis inducing factor 1 (Tiam 1) and underlying mechanisms. Methods: Using adhesion selection, two subpopulations with high ($M_H$) or low ($M_L$) invasive capacity were separated from the human gastric cancer cell line MKN-45 ($M_0$). Tiam 1 antisense oligodeoxynucleotide (ASODN) was transfected into $M_H$ cells with liposomes, and expression of Tiam 1 mRNA and protein was determined by RT-PCR and quantitative cellular-ELISA. Changes in the cytoskeleton, invasive capacity in vitro and expression of ras-related $C_3$ botulinum toxin substrate 1 (Rac 1), integrin ${\beta}1$ and matrix metalloproteinase 2 (MMP 2) between Tiam 1 ASODN transfected $M_H$ cells and non-transfected cells were observed by HE staining, cytoskeletal protein staining, scanning electron microscopy, Boyden chamber tests and cyto-immunohistochemistry. Results: A positive correlation existed between the expression level of Tiam l mRNA or protein and the invasion capacity of gastric cancer cells. After ASODN treatment ($0.43{\mu}M$ for 48 h), Tiam 1 mRNA transcription and protein expression in $M_H$ cells were decreased by 80% and 24% respectively (P < 0.05), compared with untreated controls, while invasive capacity in vitro was suppressed by 60% (P < 0.05). Morphologic and ultrastructural observation also showed that ASODN-treated $M_H$ cells exhibited smooth surfaces with obviously reduced filopodia and microspikes, which resembled $M_0$ and $M_L$ cells. Additionally, cytoskeletal distribution dramatically altered from disorder to regularity with reduced long filament-like structure, projections, pseudopodia on cell surface, and with decreased acitn-bodies in cytoplasm. After Tiam 1 ASODN treatment, the expression of Rac 1 and Integrin ${\beta}1$ in $M_H$ cells was not affected (P > 0.05), but that of MMP 2 in $M_H$ cells was significantly inhibited compared with untreated cells (P < 0.05). Conclusion: Over-expression of Tiam-1 contributes to the invasive phenotype of gastric cancer cells. Inhibition of Tiam 1 expression could impair the invasive capacity of gastric cancer cells through modulating reconstruction of the cytoskeleton and regulating expression of MMP 2.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.18 no.1
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• pp.110-122
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• 1993

When voice is transmitted through packet switching network, there needs a overload control, that is, a control for the congestion which lasts short periods and occurrs in local extents. In this thesis, we analyzed the performance of the statistical packet voice multiplexer using the overload control strategy by bit dropping. We assume that the voice is coded accordng to (4,2) embedded ADPCM and that the voice packet is generated and transmitted according to the procedures in the CCITT recomendation G. 764. For the performance analysis, we must model the superposed packet arrival process to the multiplexer as exactly as possible. It is well known that interarrival times of the packets are highly correlated and for this reason MMPP is more suited for the modelling in the viewpoint of accuracy. Hence the packet arrival process in modeled as MMPP and the matrix geometric method is used for the performance analysis. Performance analysis is similar to the MMPP IG II queueing system. But the overload control makes the service time distribution G dependent on system status or queue length in the multiplexer. Through the performance analysis we derived the probability generating function for the queue length and using this we derived the mean and standard deviation of the queue length and waiting time. The numerical results are verified through the simulation and the results show that the values embedded in the departure times and that in the arbitrary times are almost the same. Results also show bit dropping reduces the mean and the variation of the queue length and those of the waiting time.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.5C
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• pp.706-715
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• 2004

In this paper, to decide the watermark embedding positions and embed the watermark we use the subband tee structure which is presented in the wavelet domain and the edge information in the spatial domain. The significant frequency region is estimated by the subband searching from the higher frequency subband to the lower frequency subband. LH1 subband which has the higher frequency in tree structure of the wavelet domain is divided into 4${\times}$4 submatrices, and the threshold which is used in the watermark embedding is obtained by the blockmatrix which is consists by the average of 4${\times}$4 submatrices. Also the watermark embedding position, Keymap is generated by the blockmatrix for the energy distribution in the frequency domain and the edge information in the spatial domain. The watermark is embedded into the wavelet coefficients using the Keymap and the random sequence generated by LFSR(Linear feedback shift register). Finally after the inverse wavelet transform the watermark embedded image is obtained. the proposed watermarking algorithm showed PSNR over 2㏈ and had the higher results from 2％ to 8％ in the comparison with the previous research for the attack such as the JPEG compression and the general image processing just like blurring, sharpening and gaussian noise.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.17 no.3
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• pp.181-188
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• 2012

Despite the ecological importance of seagrass beds, their distributional information in Korean coastal waters is insufficient. Therefore, we used hydroacoustic system to collect accurate bathymetry and classification of seagrass, and Kompsat-2 (4 m spatial resolution) image for detection of seagrass beds at Deukryang Bay, Korea. The accuracy of Kompsat-2 image classification was evaluated using hydracoustic survey result using error matrix and Kappa value. The total area of seagrass beds from satellite image classification was underestimated compared to the hydroacoustic survey, estimated 3.9 and $4.5km^2$ from satellite image and hydroacoustic data, respectively. Nonetheless, the accuracy of Kompsat-2 image classification over hydroacoustic-based method showing 90% (Kappa=0.85) for the three class maps (seagrass, unvegetated seawater and aquaculture). The agreement between the satellite image classification and the hydroacoustic result was 77.1% (the seagrass presence/absence map). From our result of satellite image classification, Kompsat-2 image is suitable for mapping seagrass beds with high accuracy and non-destructive method. For more accurate information, more researches with a variety of high-resolution satellite image will be preceded.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.19 no.4
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• pp.81-99
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• 2020

Origin-destination data have been collected and utilized for demand analysis and service design in various fields such as public transportation and traffic operation. As the utilization of big data becomes important, there are increasing needs to store raw origin-destination data for big data analysis. However, it is not practical to store and analyze the raw data for a long period of time since the size of the data increases by the power of the number of the collection points. To overcome this storage limitation and long-period pattern analysis, this study proposes a methodology for compression and origin-destination data analysis with the compressed data. The proposed methodology is applied to public transit data of Sejong and Seoul. We first measure the reconstruction error and the data size for each truncated matrix. Then, to determine a range of principal components for removing random data, we measure the level of the regularity based on covariance coefficients of the demand data reconstructed with each range of principal components. Based on the distribution of the covariance coefficients, we found the range of principal components that covers the regular demand. The ranges are determined as 1~60 and 1~80 for Sejong and Seoul respectively.