• Title/Summary/Keyword: distance geometry

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The Structural Studies of Biomimetic Peptides P99 Derived from Apo B-100 by NMR

  • Kim, Gil-Hoon;Won, Ho-Shik
    • Journal of the Korean Magnetic Resonance Society
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    • v.24 no.4
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    • pp.136-142
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    • 2020
  • Apolipoprotein B-100 (apo B-100), the main protein component that makes up LDL (Low density lipoprotein), consists of 4,536 amino acids and serves to combine with the LDL receptor. The oxidized LDL peptides by malondialdehyde (MDA) or acetylation in vivo were act as immunoglobulin (Ig) antigens and peptide groups were classified into 7 peptide groups with subsequent 20 amino acids (P1-P302). The biomimetic peptide P99 (KGTYG LSCQR DPNTG RLNGE) out of B-group peptides carrying the highest value of IgM antigens were selected for structural studies that may provide antigen specificity. Circular Dichroism (CD) spectra were measured for peptide secondary structure in the range of 190-260 nm. Experimental results show that P99 has pseudo α-helice and random coil structure. Homonuclear (COSY, TOCSY, NOESY) 2D-NMR experiments were carried out for NMR signal assignments and structure determination for P99. On the basis of these completely assigned NMR spectra and proton distance information, distance geometry (DG) and molecular dynamic (MD) were carried out to determine the structures of P99. The proposed structure was selected by comparisons between experimental NOE spectra and back-calculated 2D NOE results from determined structure showing acceptable agreement. The total Root-Mean-Square-Deviation (RMSD) value of P99 obtained upon superposition of all atoms were in the set range. The solution state P99 has mixed structure of pseudo α-helix and β-turn(Gln[9] to Thr[13]). These NMR results are well consistent with secondary structure from experimental results of circular dichroism. Structural studies based on NMR may contribute to the prevent oxidation studies of atherosclerosis and observed conformational characteristics of apo B-100 in LDL using monoclonal antibodies.

Improvement of non-negative matrix factorization-based reverberation suppression for bistatic active sonar (양상태 능동 소나를 위한 비음수 행렬 분해 기반의 잔향 제거 기법의 성능 개선)

  • Lee, Seokjin;Lee, Yongon
    • The Journal of the Acoustical Society of Korea
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    • v.41 no.4
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    • pp.468-479
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    • 2022
  • To detect targets with active sonar system in the underwater environments, the targets are localized by receiving the echoes of the transmitted sounds reflected from the targets. In this case, reverberation from the scatterers is also generated, which prevents detection of the target echo. To detect the target effectively, reverberation suppression techniques such as pre-whitening based on autoregressive model and principal component inversion have been studied, and recently a Non-negative Matrix Factorization (NMF)-based technique has been also devised. The NMF-based reverberation suppression technique shows improved performance compared to the conventional methods, but the geometry of the transducer and receiver and attenuation by distance have not been considered. In this paper, the performance is improved through preprocessing such as the directionality of the receiver, Doppler related thereto, and attenuation for distance, in the case of using a continuous wave with a bistatic sonar. In order to evaluate the performance of the proposed system, simulation with a reverberation model was performed. The results show that the detection probability performance improved by 10 % to 40 % at a low false alarm probability of 1 % relative to the conventional non-negative matrix factorization.

The Structural Studies of Peptide P143 Derived from Apo B-100 by NMR

  • Lee, Ji-Eun;Kim, Gil-Hoon;Won, Ho-Shik
    • Journal of the Korean Magnetic Resonance Society
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    • v.25 no.4
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    • pp.58-63
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    • 2021
  • Apolipoprotein B-100 (apo B-100), the main protein component that makes up LDL (Low density lipoprotein), consists of 4,536 amino acids and serves to combine with the LDL receptor. The oxidized LDL peptides by malondialdehyde (MDA) or acetylation in vivo act as immunoglobulin (Ig) antigens and peptide groups were classified into 7 peptide groups with subsequent 20 amino acids (P1-P302). The biomimetic peptide P143 (IALDD AKINF NEKLS QLQTY) out of C-group peptides carrying the highest value of IgG antigens were selected for structural studies that may provide antigen specificity. Experimental results show that P143 has β-sheet in Ile[1]-Asn[9] and α-helice in Gln[16]-Tyr[20] structure. Homonuclear 2D-NMR (COSY, TOCSY, NOESY) experiments were carried out for NMR signal assignments and structure determination for P143. On the basis of these completely assigned NMR spectra and proton distance information, distance geometry (DG) and molecular dynamic (MD) were carried out to determine the structures of P143. The proposed structure was selected by comparisons between experimental NOE spectra and back-calculated 2D NOE results from determined structure showing acceptable agreement. The total Root-Mean-Square-Deviation (RMSD) value of P143 obtained upon superposition of all atoms were in the set range. The solution state P143 has a mixed structure of pseudo α-helix and β-turn(Phe[10] to Glu[12]). These results are well consistent with calculated structure from experimental data of NOE spectra. Structural studies based on NMR may contribute to the prevent oxidation studies of atherosclerosis and observed conformational characteristics of apo B-100 in LDL using monoclonal antibodies.

Building Detection Using Edge and Color Information of Color Imagery (컬러영상의 경계정보와 색상정보를 활용한 동일건물인식)

  • Park, Choung Hwan;Sohn, Hong Gyoo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.3D
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    • pp.519-525
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    • 2006
  • The traditional area-based matching or efficient matching methods using epipolar geometry and height restriction of stereo images, which have a confined search space for image matching, have still some disadvantages such as mismatching and timeconsuming, especially in the dense metropolitan city that very high and similar buildings exist. To solve these problems, a new image matching method through building recognition has been presented. This paper described building recognition in color stereo images using edge and color information as a elementary study of new matching scheme. We introduce the modified Hausdorff distance for using edge information, and the modified color indexing with 3-D RGB histogram for using color information. Color information or edge information alone is not enough to find conjugate building pairs. For edge information only, building recognition rate shows 46.5%, for color information only, 7.1%. However, building recognition rate distinctly increase 78.5% when both information are combined.

Interference Effects of Change in Wind Passage of a Building Group on Wind Loads and Wind Environments (건축물군의 바람길변화로 인한 풍하중 상호간섭 및 풍환경)

  • Cho, Kang-Pyo;Hong, Sung-Il;Kim, Mu-Hwan;Lee, Ok-Jin
    • Proceedings of the Computational Structural Engineering Institute Conference
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    • 2008.04a
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    • pp.402-409
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    • 2008
  • Wind loads and environments in realistic situations surrounded by neighboring buildings may be considerably different from those in idealized or simplified situations such as codes and standards. Interference effects of change in wind passage of a building group on wind loads and wind environments are reviewed. Wind-induced interference effects depend mainly on the building geometry and arrangement of these structures, their orientation and upstream terrain conditions. The most important factor among them may be the arrangement of building structures which can change the wind direction directly. Interference effects regarding wind loads are discussed with examples of window damages by typhoon and of pressure measurements in the boundary layer wind tunnel. Wind environment problems are also discussed, specially underlined on pedestrian comfort and safety. Various evaluation techniques or standards of wind environment are introduced. The change of wind velocity between the panel-type apartment buildings is examined, depending on the distance each other.

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A Sub-Wavelength Focusing Lens Composed of a Dual-Plate Metamaterial Providing a Negative Refractive Index

  • Kim, Dong-Ho
    • Journal of electromagnetic engineering and science
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    • v.12 no.1
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    • pp.26-31
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    • 2012
  • We have proposed a metamaterial lens that enables sub-wavelength focusing, which is shorter than an operating wavelength. Our lens is a two dimensional array of a unit cell consisting of a metallic dual-plate printed on a dielectric substrate. The unique dual-plate structure provides negativeness both in permittivity and permeability, with no help from conventional additional structures, which are normally printed on the opposite of metallic patterns. Therefore, we can focus a source (or an image) in a tiny distance shorter than the free space wavelength (${\lambda}$) at the frequencies of interest. Furthermore, since the proposed geometry does not need separate supplementary structures to acquire negative permittivity or permeability, our lens is much simpler than conventional metamaterial lenses, which is a strong point in practical applications. We have validated sub-wavelength focusing ability in a 6 GHz frequency band through an experiment of near field scanning, which provided the width of about 0.19 ${\lambda}$ at a half maximum of a peak value of an measured image. The width of the focused image through the lens is more than 4 times shorter than that without the lens, which confirms the validity of our design approach.

Cylindrical Object Recognition using Sensor Data Fusion (센서데이터 융합을 이용한 원주형 물체인식)

  • Kim, Dong-Gi;Yun, Gwang-Ik;Yun, Ji-Seop;Gang, Lee-Seok
    • Journal of Institute of Control, Robotics and Systems
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    • v.7 no.8
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    • pp.656-663
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    • 2001
  • This paper presents a sensor fusion method to recognize a cylindrical object a CCD camera, a laser slit beam and ultrasonic sensors on a pan/tilt device. For object recognition with a vision sensor, an active light source projects a stripe pattern of light on the object surface. The 2D image data are transformed into 3D data using the geometry between the camera and the laser slit beam. The ultrasonic sensor uses an ultrasonic transducer array mounted in horizontal direction on the pan/tilt device. The time of flight is estimated by finding the maximum correlation between the received ultrasonic pulse and a set of stored templates - also called a matched filter. The distance of flight is calculated by simply multiplying the time of flight by the speed of sound and the maximum amplitude of the filtered signal is used to determine the face angle to the object. To determine the position and the radius of cylindrical objects, we use a statistical sensor fusion. Experimental results show that the fused data increase the reliability for the object recognition.

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A Study on the Optimal Magnet for ECR (ECR 용 최적 마그네트에 관한 연구)

  • Kim, Y.T.;Kim, Y.J.;Kim, K.S.;Lee, Y.J.;Son, M.H.
    • Proceedings of the KIEE Conference
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    • 1992.07b
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    • pp.649-652
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    • 1992
  • ECR(Electron Cyclotron Resonance) occure at ${\omega}_c$=${\omega}$, ${\omega}_c$:electron cycltron frequency, ${\omega}$:electromagnetic wave frequency. ECR system have several merit, 1) power transefer efficiency 2) low neutral gas pressure (below 1 mTorr) 3) high plasma density($10^{12}$ $cm^{-3}$). It is applicated variously in the field of semiconductor and new materials as the manufacturing equipment. Magnetic field in ECR system contruct resonance layer (${\omega}$=2.45GHz, $B_z$=875 Gauss) and control plasma. Plasma is almost generated at resonance layer. If the distance between substrate and resonance layer is short, uniformity of plasma is related with profile of resonance layer. Plasma have the property "Cold in Field", so directonality of magnetic field is one of the control factors of anisotropic etching. In this study, we calculate B field and flux line distribution, optimize geometry and submagnet current and improve of magnetic field directionality (99.9%) near substrate. For the purpose of calculation, vector potential A(r,z) and magnetic field B(r,z), green function and numerical integration is used. Object function for submagnet optimization is magnetic field directionality on the substrate and Powell method is used as optimization skim.

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Design of 5.8GHz Band 4×4 Butler Matrix using Commercial 90° Hybrid Coupler (상용 90도 하이브리드 커플러를 이용한 5.8GHz 대역 4×4 버틀러매트릭스 설계)

  • Park, Byeong-Hoon
    • The Transactions of the Korean Institute of Electrical Engineers P
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    • v.63 no.3
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    • pp.200-205
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    • 2014
  • In this paper, 5.8GHz band $4{\times}4$ Butler matrix is designed using easily accessible commercial $90^{\circ}$ hybrid coupler and semirigid coaxial cable as a transmission line. This Butler matrix is very flexible to changes of antenna system specification like a frequency band because $90^{\circ}$ hybrid coupler changing is all to do. The result of design is the distance of $2{\times}2$ array antenna element is $\sqrt{2}{\lambda}/4$, the 4 beam directions are diagonal of array antenna and phase shifter is not necessary. The beam width is roughly $25^{\circ}$ narrower because of array antenna geometry and the side lobe is about 10dB higher partially than theoretical beam pattern. But the overall beam pattern is similar with theoretical beam. This Butler matrix can be applied to switching beam antenna of 5.8GHz band Wi-Fi and WAVE system.

How to Investigate Students' Zone of Proximal Development (ZPD) (학생들의 근접발달영역(ZPD)에 대한 탐구)

  • Kim, Dong--Joong
    • Journal of the Korean School Mathematics Society
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    • v.12 no.4
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    • pp.493-508
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    • 2009
  • This study investigates aspects of the zone of proximal development (ZPD), the distance between the actual development and the potential development. Out of 18 university students taking a geometry course, two students with the same actual developmental level in the van Hiele model in the pre-test and post-test were interviewed for measuring their potential developmental level. Based on the communicational approach to cognition, the characteristics of the two interviewees' discourse on 3D reflective symmetry were identified. There were considerable differences between the two interviewees in terms of their potential developmental level. Methodological implications for how to investigate students' ZPD in mathematics education research were addressed.

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