• Journal of the Korean Society of Food Science and Nutrition
• /
• v.33 no.3
• /
• pp.500-504
• /
• 2004

This study was designed to investigate the possible utilization of Zanthoxylum schinifolium as a source of decontamination agents. The antimicrobial effect of Zanthoxylum schinifolium extract was investigated against Vibrio parahaemolyticus which is food-born disease organism. Ethanol extract of Zanthoxylum schinifolium was compared with water extract of Zanthoxylum schinifolium to test antimicrobial activities against Vibrio parahaemolyticus by disk method. Ethanol extract was more effective than water ,extract on the antimicrobial activities. It had remarkable antimicrobial activities against Vibrio parahaemolyticus. It was very stable on the wide range of temperature and pH. It turned out by GC-MS that estragole (4-allyl anisole) was a major antimicrobial component of Zanthoxylum schinifolium extract. These results indicated that Zanthoxylum schinifolium extract could protect against bacterial contamination and inhibit a growth of Vibrio parahaemolyticus.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.6
• /
• pp.1042-1052
• /
• 2010

Forty-seven Salmonella Typhimurium (33 zoonotic, 14 clinical) strains were tested for antimicrobial resistance using the standard disk diffusion method. The presence of relevant resistance genes and class 1 integrons were investigated by using PCR. Pulsed-field gel electrophoresis (PFGE) and plasmid profiling were carried out to determine the genomic diversity of Salmonella Typhimurium. Approximately 57.4% of the S. Typhimurium strains were multidrug resistant (MDR) and showed high resistance rates to tetracycline (70.2%), sulfonamides (57.4%), streptomycin (53.1%), ampicillin (29.7%), nalidixic acid (27.6%), kanamycin (23.4%), chloramphenicol (21.2%), and trimethoprim (19.1%). Resistance towards cephalosporins was noted for cephalothin (27.6%), cephradine (21.2%), amoxicillin clavulanic acid (17.0%), and cephalexin (17.0%). Resistance genes, $bla_{TEM}$, strA, aadA, sul1, sul2, tetA, tetB, and tetC, were detected among the drug-resistant strains. Thirtythree strains (70.2%) carried class 1 integrons, which were grouped in 9 different profiles. DNA sequencing identified sat, aadA, pse-1, and dfrA genes in variable regions on class 1 integrons. Thirty-five strains (74.4%) were subtyped to 22 different plasmid profiles, each with 1-6 plasmids (2.0 to 95 kb). PFGE subtyped the 47 strains into 39 profiles. In conclusion, high rates of multidrug resistance were found among the Malaysian Salmonella Typhimurium strains. The emergence of multidrug-resistant Salmonella Typhimurium to cephalosporin antibiotics was also observed. The strains were very diverse and no persistent clone was observed. The emergence of MDR Salmonella Typhimurium is a worldwide problem, and this report provides information for the better understanding of the prevalence and epidemiology of MDR S. Typhimurium in Malaysia.

• Microbiology and Biotechnology Letters
• /
• v.10 no.1
• /
• pp.33-37
• /
• 1982

The work has been carried out for the development of antifungal antibiotics possessing curative effect in the control of sheath blight disease of rice plant. Soil samples were collected from over 1600 spots throughout the country. More than 1300 specimens which seem to be the genus Streptomyces were isolated from the soil samples. Screening procedures consist of respective processes by four steps. Those are growth inhibition test in liquid culture, paper disk method, dendroid test and green house test. 102 isolates appeared to be active against Pellicularia sasakii when all specimens isolated were examined by the first growth inhibition test. Finally a strain of Streptomyces forming strong antifungal substances against P. sasakii was selected from a soil sample of Mt. Soyo, Kyeongi Province. Antifungal substances formed by the strain were isolated and purified from the culture broth and examined for antimicrobial activities as to be specific against fungi but not active on bacterial growth.

• Journal of Veterinary Clinics
• /
• v.31 no.4
• /
• pp.350-353
• /
• 2014

A 13-year-old, castrated male, Shih Tzu dog with a history of acute ataxia was referred to veterinary medical teaching hospital and anesthetized for diagnostic magnetic resonance imaging of cervical intervertebral disk disease. After preanesthetic evaluation including physical examination, blood chemistry, radiography and ultrasound, the patient was premedicated with intravenous butorphanol (0.2 mg/kg). Anesthesia was induced by intravenous propofol (6 mg/kg) and maintained with isoflurane at 1.2 minimal alveolar concentrations. Because the mean arterial pressure (MAP) decreased from 70 to 58 mmHg at 70 minutes after induction, dobutamine was administered by constant rate infusion ($5{\mu}g/kg/min$) to treat hypotension. However MAP did not increase, and heart rate rapidly decreased from 100 to 55 beats per minute (bpm). To treat bradycardia, intravenous glycopyrrolate ($5{\mu}g/kg$) was administered, and heart rate increased to 165 bpm. After extubation of endotracheal tube, the patient showed normal recovery without any problems related to cardiovascular system. Unexpected dobutamine-induced bradycardia was considered as Bezold-Jarisch reflex. It is recommended that clinicians know and prepare the possibility of bradycardia during dobutamine therapy under general anesthesia.

• Journal of Korean society of Dental Hygiene
• /
• v.12 no.3
• /
• pp.631-640
• /
• 2012

Objectives : This study mean to confirm the antibacterial activity of a garlic extract widely culturing in our region and was to determine the effect of dentifrice containing 0.1% extracts of garlic on dental plaque and gingivitis in a double blind and clinical studies in 50 healthy adults aged from 20 to 22 years who provided a consent for their participation. Methods : The antibacterial activity was evaluated using triple distilled water and the Kirby-Bauer disk diffusion method and the minimal inhibitory concentration(MIC) against various pathogens for periodontal disease, such as P. gingivalis 381(ATCC33277), was estimated. The experimental groups classified according to the concentration of garlic extract used: 10,000ppm(A), 5,000ppm(B), 2,500ppm(C), 1,000ppm(D). Oral examination of subjects was performed through clinical periods and on day of baseline, 6, 12, 19, 25 days plaque index and gingival index were scored by Turesky' modified index and Loe & Silness index. After 12, 19, 25 days use of their respective dentifrices, statistically decreases of plaque index, gingival index were shown in both the experimental and the control group, respectively. Results : There was significant antibacterial activity in the "2,500ppm(C)" group against P. gingivalis 381. Experimental group exhibited significantly the lower plaque levels and the higher levels of gingival health by the use of the dentifrices contained extract of garlic from 6 days compare with control group(p<0.05). The degree of decrease was more significant on gingivitis level of the experimental group than the control group(p<0.05). Conclusions : This findings indicated that the oral products containing a garlic extract is effective in preventing and treating periodontal diseases, and has potential value in inhibiting periopathogens.

• Journal of Sensor Science and Technology
• /
• v.24 no.4
• /
• pp.239-246
• /
• 2015

Blood pressure is possible to diagnose a disease associated with blood pressure and judgment the current health of patients. Automatic blood pressure monitor capable of measuring a blood pressure easily in hospital and at home have become spread. In this study, we developed the blood pressure simulator (BPS) that can test the arm-type automatic blood pressure monitor that is commonly used in hospital. BPS is to produce a pressure similar to the pressure wave generated in the human blood using a servo disk motor. Then, using the silicon tube, it implements the situations such as human blood vessels, and to output the generated pressure waveform. Simply the BPS's phantom put on the cuff and it is able to simulate blood pressure. So anyone can quickly test the blood pressure monitor within one minute and it is possible to shorten the test time required for the automatic blood pressure monitor. In Performance test, the trends and the standard deviation of the values measured in the BPS is similar to the value of the measured pressure from people with normal blood pressure. Thus, the development BPS showed a possibility of taking into account the actual blood pressure measurement environment simulator.

• Research in Plant Disease
• /
• v.16 no.1
• /
• pp.90-93
• /
• 2010

Sclerotinia rot occurred sporadically on the stems and leaves of Astragalus sinicus in the farmers fields at Goseong-gun, Gyeongnam province in Korea. The infected plants showed the typical symptoms: watersoaked, wilt, rot, blight and eventual death. The colony of the isolated fungus on potato-dextrose agar (PDA) was white to faintly gray color. Sclerotia formed on the PDA were globose in shape, black in color and $2{\sim}14{\times}2{\sim}7mm$ in size. The optimum temperature for mycelial growth and sclerotium formation was at $20^{\circ}C$ on PDA. Apothecia formed on PDA were globose~disk in shape and 3~8 mm in size. Asci were cylindrical in shape and $145{\sim}210{\times}10{\sim}12{\mu}m$ in size. Ascospores were ellipsoid and $10{\sim}14{\times}6{\sim}7{\mu}m$ in size. On the basis of mycological characteristics and pathogenicity test on host plants, the fungus was identified as Sclerotinia trifoliorum Eriksson. This is the first report on sclerotinia rot of A. sinicus caused by Strifoliorum Eriksson in Korea.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.20 no.2
• /
• pp.304-310
• /
• 2010

Chronic pain and herniated disk is a common disease that 80% of adults are experienced. There diseases rates of caused by the physical shock, such as the traffic accident, and the accidental fall is about 10%. And the most of these diseases is caused by having habitual incorrect position. People know that incorrect position would cause to accumulate continuous stress, but it is not easy to correct position. Because it does not recognize incorrect position repeated habitual consequently. This system collects data of user position after sensors that could measure position attach on use and presumes correct position used by position presumption algorithms. Its system purpose is continuing incorrect position could be aware to user and lead to change to correct position to prevent habituation of incorrect position. If habitual of correct position continues through accurate measurement and repeating cognitive learning, it would help for children and chronic patience.

• The Journal of Korean Academy of Orthopedic Manual Physical Therapy
• /
• v.12 no.2
• /
• pp.33-41
• /
• 2006

This study planed to analyze durability of effect and result that the Medx and Sling exercise gets to the strength of lumbar extensors. 15 patients who had enforce the minimal invasive lumbar surgery were executed 3 times per week for 8 weeks. The purpose of this study was to identify the influence of the mixed exercises of the Medx and Sling program on lumbar trunk muscles and to present basic data for the proper exercise prescription for lumbar patients. The results were as follows: 1) Left: Patients by lumbar hernia operation of trunk muscle strength by Sling-exercise: 0, 45, 90, 135, 180 (degree) treatment periods (P<0.05). 2) Right: Patients by lumbar hernia operation of trunk muscle strength by Sling-Exercise: 0, 45, 90, 135, 180 (degree) treatment periods (P<0.05). Medx treatment, a muscle strengthening lumbar extension exercise program, was now being used at local hospitals. In addition, Sling exercise, which is designed to develop lumbar muscle by way of reducing gravity in a new way so that it can accelerate the growth of muscles and ligaments in-depth in the patients, also has begun to be introduced gradually. In this study, therefore, the new mixed program (Sling and Medx training)can used as an exercise program that can reduce pain and increase lumbar muscles, not only for disk disease patients but also for all those who have undergone surgery or who haven't undergone surgery, who have chronic pain, and it also can be utilized as basic data for the new method of exercise.

• Korean Journal Plant Pathology
• /
• v.14 no.6
• /
• pp.646-650
• /
• 1998

Mycological characteristics of Phytophthora nicotianae var. nicotianae SPC10 (A1 type) causing Phytophthora rot of strawberry and the resistances of 11 strawberry cultivars against the pathogen were examined. Optimum temperature for the mycelial growth of the pathogen was obtained in the range of 30~35$^{\circ}C$, and the growth was completely stopped under 13$^{\circ}C$ or over 42$^{\circ}C$. Aerial mycelia were abundant on oatmeal agar (OMA), V-8 juice agar (V8A) and lima bean agar (LBA) medium, although there were slight differences, however, on cornmeal agar (CMA) medium, it was a shape of stellate without aerial mycelia. The colony shape on potato dextrose agar (PDA) medium was rough and irregular whereas the mycelial growth was slow, and some aerial mycelia were only produced in the middle of PDA medium. Optimum temperature for sporangial formation was 3$0^{\circ}C$, and zoospores were mostly released at $25^{\circ}C$ from the sporangia. Sporangia were more produced in C/Z solution with pH 5. 0~6.$0^{\circ}C$ than sterilized distilled water (DSW) and distilled water (DW), and zoospores were also released much more than other solutions. Eleven strawberry cultivars such as Reiko, Hokowase, Eyeberry, Akaneko, Sistakara, Toyonoka, Nyoho, Sulhong, Suhong, Myhong and Wonkyo #3104 revealed the disease incidence up to 88.9~100% by the leaf inoculation with mycelial disk. However, Nyoho and Suhong showed higher level of resistance against the pathogen by root inoculation.