• Journal of Microbiology and Biotechnology
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• v.24 no.12
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• pp.1644-1653
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• 2014

Wuyiencin is produced by Streptomyces ahygroscopicus var. wuyiensis CK-15 and is widely used as an antifungal agent in agriculture. Analysis of wuyiencin biosynthetic gene clusters reveals wysR, a member of the LAL-family of transcriptional regulatory genes. WysR consists of an N-terminal PAS domain and a LuxR family C-terminal helix-turn-helix motif. However, the roles of wysR in wuyiencin biosynthesis are largely unknown. In this study, we showed that inactivation of wysR resulted in the complete loss of wuyiencin production, which could be restored by complementation with a single copy of wysR. Furthermore, we successfully increased wuyiencin production to a significantly higher level by overexpression of wysR in S. wuyiensis CK-15. Quantitative real-time RT-PCR analysis showed that WysR regulates wuyiencin biosynthesis by modulating other putative regulatory genes. Thus, WysR was identified as an activator of wuyiencin biosynthesis, and overexpression of wysR gene proved to be an effective strategy for improving wuyiencin production.

• Korean journal of applied entomology
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• v.56 no.1
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• pp.41-49
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• 2017

The community of xylophagous beetles belonging to Cerambycidae, Curculionidae and Scolytinae in Korean white pine, Pinus koraiensis Siebold & Zuccarini, forests was surveyed using Malaise traps in 2007. A total of 1,615 xylophagous beetles were collected, including 184 cerambycids from 15 species, 185 curculionids from 17 species, and 1,246 scolytid beetles from 6 species, of which the dominant species was the ambrosia beetle Xyleborus mutilatus Blandford. Ranked by order of population size, the wood-boring and bark beetle community in Korean white pine showed high dominance by one species of Scolytinae, suggesting the community had low biological diversity. Thinning in Korean white pine forests influenced on the abundance of bark and ambrosia beetles, whose populations in particular stands increased 1 year after thinning, and then decreased the following year.

• 한국균학회소식:학술대회논문집
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• 2006.10a
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• pp.10-12
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• 2006

• The Plant Pathology Journal
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• v.29 no.3
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• pp.344-349
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• 2013

Intraspecific variation in morphometry of pine wood nematodes Bursaphelenchus xylophilus and B. mucronatus in relation to geographical locations in South Korea was investigated using morphometric characters (body length, a, b and c ratio, stylet length, and spicule length for a male nematode and V (%) value for a female nematode). B. xylophilus was isolated from Pinus thunbergii in Jinju (1998), Ulsan (2000), Yangsan (2000), Mokpo (2001) and Jeju (2004), and from P. densiflora in Gumi (2001). B. mucronatus was isolated from P. thunbergii in Jinju (1991) and from P. densiflora in Milyang (2001). The body length of male and female B. xylophilus had the highest coefficient of variability and showed significant differences among geographical locations. The V (%) value for female B. xylophilus showed the lowest coefficient of variability, changing little with geographical area and host plant. All morphometric characters in B. mucronatus except for stylet length and female body length showed no significant differences between locations or hosts, suggesting they may not be affected by geographical area or host plant.

• Journal of Korean Society of Forest Science
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• v.94 no.5 s.162
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• pp.334-337
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• 2005

Pine wood nematode isolates from Haman, Gyoungsangnam-do were cryopreserved with three different cryoprotectants (glycerol, ethylene glycol and dimethylsulfoxide), Nematode Growth medium (NGM) and M 9 buffer solution, respectively, and then survival rate, reproduction ability, and pathogenicity of stored nematodes were compared. Survival rates of juvenile was excellent in 15% glycerol solution as $72{\pm}5.3%$. The survival ability of nematodes at juvenile stage was more efficient than that of nematodes at adult stage. Pre-incubation treatment at $4^{\circ}C$ before storing in liquid nitrogen didn't affect the survival ability of pine wood nematode. When the reproduction ability of nematodes was confirmed on Botrytis cinerea agar plates at $28^{\circ}C$ incubator, there was no difference between cryopreserved and non-cryopreserved. Pathogenic ability of cryopreserved nematodes was also evaluated and confirmed by artificially inoculating them on 2-year old red pine seedlings.

• The Plant Pathology Journal
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• v.32 no.4
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• pp.340-346
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• 2016

To understand how Bursaphelenchus xylophilus kills pine trees, the differences between the effects of B. xylophilus and B. mucronatus on pine trees are usually compared. In this study, the migration and attacking ability of a non-pathogenic B. mucronatus in Pinus thunbergii were investigated. The distribution of B. mucronatus and the number of dead epithelial cells resulting from inoculation were compared with those of the pathogenic B. xylophilus. Although B. mucronatus is non-pathogenic in pines, its distribution pattern in P. thunbergii was the same as that of B. xylophilus. We therefore concluded that the non-pathogenicity of B. mucronatus could not be attributed to its migration ability. The sparse and sporadic attacking pattern of B. mucronatus was also the same as that of B. xylophilus. However, the number and area of the dead epithelial cells in pine cuttings inoculated with B. mucronatus were smaller than in those cuttings inoculated with B. xylophilus, meaning that the attacking ability of B. mucronatus is weaker than that of B. xylophilus. Therefore, we concluded that the weaker attacking ability of B. mucronatus might be the factor responsible for the non-pathogenicity.

• Journal of Korean Society of Forest Science
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• v.95 no.3
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• pp.256-260
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• 2006

This study was conducted to investigate the effect of parasititoids on the density-fluctuation of the pine needle gall midge, Thecodiplosis japonensis. The density of T. japonensis and its parasitoids was observed by emergence trap in pine forest in Namsung-dong, Keumho-eup, Youngcheon-shi, Kyeongsangbuk-do, Korea, from 1986 to 2005. After 1987, density fluctuations of adults pine needle gall midge and its parasitoids showed similar trends and density of parasitoids was dependent on density of host population. Sex ratio of the pine needle gall midge, T. japonensis, was female-biased and had some variations in according to years. These trends were similar to those of parasitoid populations and sex ratio of Inostemma seoulis was more female-biased than that of I. matsutama. T. japonensis adults emerged in the field from May 16 to July 27 and the peak of emergence was between late May and early June. The adults of Inostemma matsutama emerged from May 16 to June 11 (peak between late May and early June), whereas those of Inostemma seoulis emerged from June 9 to July 27 (peak: late June).

• Korean journal of applied entomology
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• v.44 no.2
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• pp.157-159
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• 2005

The number of winter buds oviposited by Drycosmus kuriphilus against chestnut varieties was lower of Ishizuchi and Daap than that of Okkwang, Tsukuba and Arima. D. kuriphilus oviposited multiful eggs in each winter bud of most of the chestnut varieties tested. More than three eggs were oviposited on winter bud of lower shoot of native, Tsukuba, Okkwang and Pyounggi varieties. However, average laid eggs less than one were on winter buds of Tshizuchi and Daap. Number of eggs oviposited in winter bud on lower shoot was higher than those on upper one. More than 15 eggs were oviposited in Tsukuba, whereas one or less egg was oviposited in Daap. Rates of winter buds oviposited by D. kuriphilus in native, Arima and Tsukuba were 83.8, 78.5, and $75.9\%$, respectively.

• Korean journal of applied entomology
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• v.44 no.3 s.140
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• pp.219-223
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• 2005

Torymus geranii was found to be a ectoparasitoid attacking the larvae of Dryocosmus kuriphilus. T. geranii has longevity of a $42.8{\pm}9.8$ days at $20^{\circ}C$ and $26.5{\pm}11.0$ day at $25^{\circ}C$ under conditions supplied with 100% honeydew. Oviposition numbers at 20 and $25^{\circ}C$ were $22.3{\pm}12.5$ and $42.2{\pm}18.4$, respectively. The eggs were oval in shape, measuring $0.56{\pm}0.33$ mm in length. The larvae, $2.94{\pm}0.18mm$ in length, was white and length of male and female pupae were $2.01{\pm}0.18mm$ and $2.73{\pm}0.09mm$, respectively. T. geranii had two generations with overwintering generation emerged on late May to early June at Chunchon, central part of Korea, however showed three generations with overwintering generation emerged on middle May to early June, the first one on late June to early July, and the second one on late July to earlyl August.

• Molecules and Cells
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• v.28 no.3
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• pp.155-165
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• 2009

The newly sequenced complete mitochondrial genome of the brown marmorated stink bug, Halyomorpha halys($St{\aa}l$) (Hemiptera: Pentatomidae), is a circular molecule of 16,518 bp with a total A+T content of 76.4% and two extensive repeat regions in A+T rich region. Nucleotide composition and codon usage of H. halys are about average when compared with values observed in 19 other published hemipteran mitochondrial genomes. Phylogenetic analyses using these 20 hemipteran mitochondrial genomes support the currently accepted hypothesis that suborders Heteroptera and Auchenorrhyncha form a monophyletic group. The mitochondrial gene arrangements of the 20 genomes are also consistent with our results.