• The Plant Pathology Journal
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• v.36 no.5
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• pp.459-467
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• 2020

Areca palm yellow leaf (AYL) disease caused by the 16SrI group phytoplasma is a serious threat to the development of the Areca palm industry in China. The 16S rRNA gene sequence was utilized to establish a rapid and efficient detection system efficient for the 16SrI-B subgroup AYL phytoplasma in China by loop-mediated isothermal amplification (LAMP). The results showed that two sets of LAMP detection primers, 16SrDNA-2 and 16SrDNA-3, were efficient for 16SrI-B subgroup AYL phytoplasma in China, with positive results appearing under reaction conditions of 64℃ for 40 min. The lowest detection limit for the two LAMP detection assays was the same at 200 ag/μl, namely approximately 53 copies/μl of the target fragments. Phytoplasma was detected in all AYL disease samples from Baoting, Tunchang, and Wanning counties in Hainan province using the two sets of LAMP primers 16SrDNA-2 and 16SrDNA-3, whereas no phytoplasma was detected in the negative control. The LAMP method established in this study with comparatively high sensitivity and stability, provides reliable results that could be visually detected, making it suitable for application and research in rapid diagnosis of AYL disease, detection of seedlings with the pathogen and breeding of disease-resistant Areca palm varieties.

• Food Science and Industry
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• v.49 no.3
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• pp.2-11
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• 2016

As recently many studies on the relationship between excessive sugar intake and chronic disease have been reported across the world, more attention to sugar intake has been paid. Sugars, called simple sugars, is a kind of carbohydrate and sum of monosaccharides and disaccharides. Sugars that are particularly related to health are added sugars or free sugars, which are added to food by processing or cooking. It is generally not to give satiety and increases energy density without other nutrients. Especially, the major food source of added sugar is sugar-sweetened beverages(SSBs), from which sugars are closely linked with health outcome. The total sugar intake in Korea is 61.4 g per day and it is almost half of those in the western countries such as the U.S. or Australia and it is within the recommendation level by the Dietary Reference Intakes for Koreans. However, when it is classified by age groups or food sources, sugar intake in adolescents and young adults are high and the sugar from processed foods is also high that would lead to high intake levels of added sugar. Particularly, the frequency of SSBs, the major food source of added sugar, is the highest among 20's. While the studies of excessive sugar intake with chronic diseases including obesity, type 2 diabetes, metabolic disease, cardiovascular disease reported in the Western populations, those in the Korean populations have just started. Given our dietary practice is very different from those in the western population, more prospective studies would be necessary to evaluate sugar intake by type or food source exactly and examine its relationship with chronic diseases in the Korean population.

• Korean Journal of Human Ecology
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• v.10 no.2
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• pp.205-213
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• 2001

A survey was conducted to investigate the intake pattern of health supplements and health foods by using questionnaire. The data were collected from 395 adults over middle age and analyzed with SAS program. The natural foods were most frequently taken among health supplements and health foods for health. There was no difference in pattern of health supplements or health foods intake between male and female. The item taken over 20% of subjects was health drinks, green tea, ginseng product, dietary fiber drink, honey, general tea, tonic medicine from natural foods, vitamin B, vitamin C from nutrition supplements, and Lactobacillus product from health supplements. As the main reason for taking health supplements or health foods, the male indicated recovery of fatigue and improvement of health, but the female indicated recovery of fatigue and protection or treatment on disease(p<0.001). The frequency of taking health supplements or health foods was once a day. About the effect of health foods 70.1%, of subjects answered not bad and 25% responded very helpful for their health. Twenty percent of subjects experienced side effects such as diarrhea. The results showed that the proper education program should be developed for consumer to choose suitable health supplements or health foods according to their dietary life and health condition.

• Parasites, Hosts and Diseases
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• v.56 no.4
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• pp.313-324
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• 2018

This article reviews Leptotrombidium deliense, including its discovery and nomenclature, morphological features and identification, life cycle, ecology, relationship with diseases, chromosomes and artificial cultivation. The first record of L. deliense was early in 1922 by Walch. Under the genus Leptotrombidium, there are many sibling species similar to L. deliense, which makes it difficult to differentiate L. deliense from another sibling chigger mites, for example, L. rubellum. The life cycle of the mite (L. deliense) includes 7 stages: egg, deutovum (or prelarva), larva, nymphochrysalis, nymph, imagochrysalis and adult. The mite has a wide geographical distribution with low host specificity, and it often appears in different regions and habitats and on many species of hosts. As a vector species of chigger mite, L. deliense is of great importance in transmitting scrub typhus (tsutsugamushi disease) in many parts of the world, especially in tropical regions of Southeast Asia. The seasonal fluctuation of the mite population varies in different geographical regions. The mite has been successfully cultured in the laboratory, facilitating research on its chromosomes, biochemistry and molecular biology.

• Journal of Veterinary Clinics
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• v.27 no.3
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• pp.220-224
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• 2010

Bovine viral diarrhea virus (BVDV) is an important worldwide disease in the livestock industry. To characterize BVDV circulating in Gyeongbuk province in the Republic of Korea which has the highest cattle population density, 365 rectal swabs from clinically BVDV Korean indigenous calves were collected. Fifty cases were identified as positive for BVDV. A phylogenetic analysis of 5'- untranslated regions (UTR) revealed that most of our cases belonged to BVDV-2a (n = 48), while only two cases were classified as BVDV-1a (n = 1) and 1b (n = 1), respectively. These results indicated that BVDV-2a is the most prevalent subgroup in Korean indigenous calves of Gyeongbuk province.

• Korean Journal of Human Ecology
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• v.7 no.2
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• pp.13-30
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• 2004

The purpose of this study was to investigate the status of teacher's perception about the nutrition education of elementary schooL The results of this study were as follows. Most of the teachers responded that nutrition education necessitate in curriculum of elementary school, the main reason for necessity of nutrition education was for proper growth, they thought starting period of nutrition education was to begin when children are in a kindergarten and a infant home. The perception for a suitable person to teach the nutrition education showed dietician, parents, class teacher and a related teacher in oder and the time of that is a related class, as a spare time at everyday, a special activity time, and service a meal in order. Importances of the contents of a nutrition education were proper eating habits, growth and nutrition, a food hygiene and a disease in order. Problems of elementary students' nutrition were a unbalanced eating, a ingestion a processing and instant food too much, lack of table manner, fatness and a weak child in order. The most of expected effects through the nutrition education were a good table habits and manners, the way of a efficient nutrition education were a need of link with parents, a need of link the dietician. a teacher's class and training in order. The average score of nutrition knowledge was 6.3$\sim$6.9 out of 10 and teachers of Gwangju scored more higher on nutrition knowledge than teachers of Jeonnam, as they arranged a dietician was so.

• Journal of Microbiology
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• v.39 no.2
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• pp.133-138
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• 2001

We investigated the antagonism of indigenous bacteria isolated from stressed mussels and their extracellular metabolites on the adult zebra mussel, Dreissena polymorpha. Selective bacterial isolates including Aeromonas media, A. salmonicida, A. veronii, and Shewanella putrefaciens, showed strong lethality against adult mussels and 100% mortality was observed within 5 days of incubation. Bacterial metabolites, fractionated and concentrated from stationary-phase culture supernatants of these bacterial isolates, displayed varying degrees of antagonistic effects on zebra mussels. Among the three size fractions examined, <5, 5-10, and >10 kDa, the mast lethal fraction seems to be >10 kDa for three of the four isolates tested. Further chemical analyses of these size fractions revealed that the predominant constituents were polysaccharides and proteins. No 2-keto-3-deoxyoctanoic acid (2-KDO), deoxyribonucleic acids (DNA) or uranic acid were detectable. Extraction of supernatants of two antagonistic isolates with polar solvent suggested that polar molecules are present in the active fraction. Our data suggest that extracellular metabolites produced by antagonistic bacteria are also involved in disease development in zebra mussels and elucidation of the mechanisms involved may offer a novel strategy for control of biofouling invertebrates.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.3 no.4
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• pp.221-226
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• 2022

A filarial nematode was found in a blood sample of an Anas falcata individual collected in South Korea in 2018. Phylogenetic analysis based on partial cytochrome C oxidase subunit I (COI) sequences placed the nematode as a novel genus of the family Onchocercidae and as closely related to Mansonella species, Chandlerella quiscali, and filarial nematodes recently reported in avian species. However, different phylogenetic relationship was observed in the NADH dehydrogenase subunit 5 and 12S rRNA-based phylogenetic trees, which might indicate the filarial nematode found in this study was not defined to belong to the known specific genera of the family Onchocercidae. The screening of 105 additional avian blood samples retrieved only one 12S rRNA-targeting polymerase chain reaction (PCR)-positive sample, which indicates that filarial nematode infection is rare in wild birds or that it occurs below the detection limit of PCR in blood samples. Nevertheless, considering the recent findings about ancient interactions between birds and human pathogenic filarial nematodes and their pathogenic potential in several avian species, additional exploration of novel filarial nematodes in wild birds remains necessary.

• KSBB Journal
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• v.30 no.6
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• pp.313-318
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• 2015

${\delta}$-Aminolevulinic acid (ALA) is an endogenous metabolite formed in the mitochondria from succinyl-CoA and glycine, and plays a key role in the living body as an intermediate of the compound in the porphyrin biosynthesis pathway. ALA has been commonly used in photodynamic therapy for several years, because ALA is of interest as a biodegradable mediator, a growth regulator, and an effective agent used in dermatology. Here, we determined which ALA derivatives were the most effective for the inhibition of the cell proliferation and growth of human fibroblast. As a result, we found that the treatment of ALA derivatives including ALA, ALAP (ALA phosphate salt), MAL (Methyl 5-aminolevulinate hydrochloride salt), PBGL (phophobilinogen lactam) and PBGH (phophobilinogen-HCl) could attenuate cell proliferation of human fibroblast cells. Among them, PBGH was the most effective derivative. In addition, PBGH treatment could induce mitochondrial membrane depolarization, leading to cell death of human fibroblast. These results suggest that mitochondrial membrane depolarization induced by ALA and PBGH treatment might be responsible for inhibition of cell proliferation and death. Taken together, our results propose the possibility that PBGH can be used as one of the effective drugs in human skin disease, psoriasis.

• Journal of Environmental Impact Assessment
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• v.15 no.5
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• pp.323-337
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• 2006

This work discussed the species composition, phylogeny, spatio-temporal distribution, ecology and natural history of North Korean Pinaceae or pine tree family, which seems to be important to maintain nature and ecosystem in the Korean Peninsula. Out of five genera and sixteen species of Pinaceae of the Korean Peninsula, North Korea contains four genera and eleven species of Pinaceae, including Pinus densilflora, P. koraiensis, P. pumila, Picea jezoensis, P. koraiensis, P. koraiensis var. koraiensis, P. pungsanensis, Larix gmelini, L. gmelinii var. olgensis, Abies holophylla and A. nephrolepis. In terms of phylogeny Pinus is closely related to Picea, and followed by Larix. Abies is close to Tsuga which only occur at Ullung Island. Distributional pattern of North Korean Pinaceae can be classified into four types; three species of nation-wide montane type i.e., Pinus densilflora, P. koraiensis and Abies holophylla, four species of central and northern subalpine type, i.e., Pinus pumila, Picea koraiensis, Larix gmelini and Abies nephrolepis, one nation-wide subalpine type, Picea jezoensis, and three species disjunctive to north type, i.e., Picea koraiensis var. koraiensis, P. pungsanensis, and Larix gmelinii var. olgensis. Pinaceae species occurring on the alpine and subalpine belts of North Korea, such as Pinus koraiensis, P. pumila, Picea jezoensis, P. koraiensis, P. koraiensis var. koraiensis, P. pungsanensis, Larix gmelini, L. gmelinii var. olgensis and A. nephrolepis are considered as the glacial descendant from the boreal region. Those species might have migrated from the north during the Pleistocene glacial epochs in search of favourable condition, and since the Holocene period they survived on the hostile alpine and subalpine environments, in which they are more competitive than warmth-tolerant temperate vegetation. Certain species, such as Picea pungsanensis, is segregated on the isolated mountains since the Pleistocene period, and forced to adapt to local environment, and eventually became an endemic species of North Korea. Recent rapid global warming trend especially in northern high mountains of North Korea could cause an unfavourable environment for the survival of cold-tolerant Pinaceae of the alpine and subalpine belts. Pinus densiflora, which is occurring on the montane belt might faced with difficulties due to both the deforestation and the outbreak of insect-borne disease, such as Bursaphelenchus xylophilus.