• Proceedings of the Korean Aquaculture Society Conference
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• 2006.05a
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• pp.281-282
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• 2006

• Proceedings of the Korean Aquaculture Society Conference
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• 2006.05a
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• pp.397-398
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• 2006

• Proceedings of the Korean Aquaculture Society Conference
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• 2006.05a
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• pp.395-396
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• 2006

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.317-322
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• 2011

This study was performed to obtain the basic data on physiological responses of low water temperature stress of the cultured Pacific abalone, Haliotis discus hannai. Abalones were exposed at low water temperatures of $7^{\circ}C$ and $4^{\circ}C$. We have investigated survival rate, superoxide dismutase (SOD) activity and total protein (TP) in the abalone by the exposure times (0, 3, 6, 12, 24, 72, 120, 168 and 216 hours). Survival rate of the abalone at $7^{\circ}C$ experiment was 90.8%, whereas at $4^{\circ}C$ experiment was 0% after exposure 10 days. SOD activity was significantly increased until 12 hours after exposure to $4^{\circ}C$, and then was recovered to starting level after 24 hours. However, there was no significant difference between control ($12^{\circ}C$) and $7^{\circ}C$ experiments. TP was significantly increased until 216 hours after 24 hours at $4^{\circ}C$ experiment, but $7^{\circ}C$ experiment showed no significant differences compared to control ($12^{\circ}C$) experiment. Therefore, H. discus hannai was acclimated in low water temperature stress at $7^{\circ}C$, but at $4^{\circ}C$, all abalone died possibly because they exceed the limits of defense ability to too low temperature.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.2
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• pp.139-145
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• 2009

We cloned and sequenced the open reading frame (ORF) cDNA encoding myostatin from the muscle of abalone (Haliotis discus hannai). The ORF cDNA of the abalone myostatin is 1134 bp and encoded 377 amino acid residues that were 60-96% homologous with the amino acids of other organism myostatins. In addition, the ORF contained a conserved proteolytic cleavage site (RXRR) and nine conserved cysteine residues in the C-terminus. Semi-quantitative RT-PCR revealed the presence of myostatin mRNA in various tissues. The strongest expression was observed in the mantle of female abalone, and the gills and heart of male abalone.

• Journal of Aquaculture
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• v.12 no.3
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• pp.229-236
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• 1999

in the present study, the zinc toxicity to larval development and seed attachment of the abalone, Haliotis discus hannai was obtained under continuous flow through system. The zinc concentration melted from zinc coating pipe for 7 months ranged from $89.00\pm2.55 \mu\textrm{g}/\ell to 15.23\pm2.58\mu\textrm{g}/\ell(Y=0.85M^2-19.71+109.96)$. Treatments were carried out with zinc concentration $0~160 \mu\textrm{g}/\ell$. The maximum and minimum of fertilization rate were $87.7\pm5.3%$ in control, $83.7\pm7.6%$ in zinc concentration $160\mu\textrm{g}/\ell$, respectively. The maximum and minimum of hatching rate were $87.5\pm4.5%$ in zinc concentration $10\mu\textrm{g}/\ell$, $79.3\pm5.6%$ in zinc concentration $160\mu\textrm{g}/\ell$, respectively. Both of the results were not significantly different (P>0.05). But the normality rate, setting rate and survival rate of abalone larvae at over zinc concentration TEX>$20\mu\textrm{g}/\ell$ decreased rapidly and showed significantly different from those of the other group(P<0.05).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.2
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• pp.174-178
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• 2014

In this study, 6-10 clinically healthy farmed abalone, Haliotis discus hannai were obtained from Jeollanam-Do Wando Soan-myeon, Bogil-myeon, and Nohwa-eup monthly for 1 year 7 months and were examined histopathologically. As a result, the adductor muscle had severe cellular swelling and myonecrosis. These lesions were stained with hematoxylin and eosin and Giemsa. The lesions were not caused by infection of bacteria or parasites. We investigated the relationship between lesion frequency and water temperature and discovered that increased water temperature was associated with increasing lesion frequency. As water temperature is related to growth rate, increased growth rate was closely related to increased lesion frequency. We considered that these lesions could be a useful factor in measuring shellfish health.

• Fisheries and Aquatic Sciences
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• v.18 no.2
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• pp.159-164
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• 2015

A feeding experiment was conducted to determine the effect of dietary distillers dried grain (DDG) on the growth and body composition of juvenile abalone, Haliotis discus hannai. Three diets were formulated to contain 0% (DDG0), 30% DDG (DDG1) replacing wheat flour and 30% DDG (DDG2) replacing fish meal and wheat flour. A commercial feed (CF) was also compared with experimental diets. Three replicate groups of abalone averaging $0.6{\pm}0.01g$ were fed one of the four diets for 14 weeks. The highest survival rate was observed in the abalone fed the DDG2 diet, which was higher than that of abalone fed the DDG0 diet. Weight gain, shell length, shell width and soft body weight of the abalone were not affected by dietary DDG and CF diet (P > 0.05). Proximate and amino acid composition of the soft whole body were not affected by dietary DDG and CF diet (P > 0.05). The result of this experiment suggests that DDG is a good ingredient to replace fish meal and wheat flour in the diet and could be used up to 30% in the diet without negative effects on the growth performance of juvenile abalone Haliotis discus hannai.

• Fisheries and Aquatic Sciences
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• v.17 no.3
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• pp.385-390
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• 2014

Abalone immortal cell lines can be used to study the physiological properties and disease mechanisms of abalone at the cellular and molecular level. As a first step for the final goal to establish abalone immortal cell lines, we examined various initial culture conditions for primary cell populations derived from Haliotis discus hannai radula tissue. The survival rate after cell isolation procedures using the enzymatic method was as low as $9.95{\pm}2.37%$. Based on three different experimental conditions for H. discus hannai radula-derived cell culture, we found that the salinity of the media and the presence of growth-promoting factors were important to support radula-derived primary cell populations during the initial culture. The growth factor-containing media adjusted to 35 psu salinity could induce 100% (8 out of 8 trials) initial cell attachment, and the rate of cell attachment reached 50-70%. The data obtained from this study will provide useful information for developing immortal cell lines from abalone species.

• The Korean Journal of Malacology
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• v.19 no.2
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• pp.81-87
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• 2003

The digestive system of the abalone, Haliotis discus hannai consists of radula sac, esophagus, crop, stomach, intestine (anterior, mid and posterior intestine) and hepatopancreas. The epithelial layer was composed of ciliated columnar cells, mucous cells and granular cells. And epithelium thickness of the crop was thicker (90.80 ${\mu}$m) than those of other regions. Mucous cells of PAS positive in the esophagus were more advanced than those of other regions. The contents of mucous cell were neutral and acid mucosubstance in the esophagus and the anterior, mid and posterior intestine. And it seem to be lipid in the crop and stomach.