• 대한약침학회지
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• 제19권3호
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• pp.225-230
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• 2016

Objectives: Mellitine, a major component of bee venom (BV, Apis mellifera), is more active against gram positive than gram negative bacteria. Moreover, BV has been reported to have multiple effects, including antibacterial, antivirus, and anti-inflammation effects, in various types of cells. In addition, wasp venom has been reported to have antibacterial properties. The aim of this study was to evaluate the antibacterial activity of BV against selected gram positive and gram negative bacterial strains of medical importance. Methods: This investigation was set up to evaluate the antibacterial activity of BV against six grams positive and gram negative bacteria, including Staphylococcus aureus (S. aureus), Salmonella typhimurium, Escherichia coli (E. coli) O157:H7, Pseudomonas aeruginosa, Burkholderia mallei and Burkholderia pseudomallei. Three concentrations of crude BV and standard antibiotic (gentamicin) disks as positive controls were tested by using the disc diffusion method. Results: BV was found to have a significant antibacterial effect against E. coli, S. aureus, and Salmonella typhyimurium in all three concentrations tested. However, BV had no noticeable effect on other tested bacteria for any of the three doses tested. Conclusion: The results of the current study indicate that BV inhibits the growth and survival of bacterial strains and that BV can be used as a complementary antimicrobial agent against pathogenic bacteria. BV lacked the effective proteins necessary for it to exhibit antibacterial activity for some specific strains while being very effective against other specific strains. Thus, one may conclude, that Apis mellifera venom may have a specific mechanism that allows it to have an antibacterial effect on certain susceptible bacteria, but that mechanism is not well understood.

• 한국식품위생안전성학회지
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• 제13권3호
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• pp.305-312
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• 1998

서울시내 약수에서 Pseudomonas속균의 균종분포, 생화학적 특성, 혈청학적 특성 및 항생제 감수성을 조사하기 위하여 약수 57개소에 대하여 봄, 여름, 가을에 걸쳐 조사하였다. 총 57개 약수를 조사한 결과, 33개 (57.9%)의 약수에서 Pseudomonas속균이 검출되었다. 봄, 여름, 가을에 모두 검출된 곳은 1곳(1.8%) 이었으며, 2회에 걸쳐 검출된 곳은 9곳 (15.8%)이었으며, 1회만 검출된 곳은 13곳 (22.8%)이었다. 지역별로는 청계산이 50%의 검출율을 나타내었으며, 북한산, 35.7&, 대모산 33.3%, 도봉산 29.6%, 수락산 25.9%, 우면산 22.2%, 불암산 7.4% 순이었다. 분리된 균종은 Ps. putidark 22주(50%)로 가장 많았으며, Ps. aeruginosa, Ps. fluorescens 및 Ps. mendocins 각 6주(13.6%), Ps.aureofaciens 4주(9.1%)이었다. 분리된 Ps. aeruginosa6주의 혈청형은 모두 5종으로 A형 2주 , B, C,E 및 G형 각 2주씩이었다. 분리된 Ps. aeruginosa의 항생제에 대한 내성은 Amoxicillin 90.9%, Chloramphenicol 84.1%, Carbenicillin 81.8%, Nalidixic acid 68.2%, Neomycin 38.6%, Streptomycin 31.8%, Gentamicin 4.6%, Kanamycin 4.6% 그리고 Colistin 2.3%이었다. Ps.aeruginosa는 다른 슈도모나스균에 비해 carbenicillin에 대한 감수성이 있으나 kanamycin에 대한 내성이 높았다. Ps. aureofaciens는 다른 슈도모나스균에 비해 streptomycin에 대하여 내성이 없었다. 분리된 Ps.aeruginosa의 항생제에 대한 다제내성양상은 5제내성이 31.8%로 가장 높았으며, 내성양상에서는 NA-CB-CTE-AMC가 18.2%로 가장 높게 나타났다.