• Journal of Plant Biology
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• v.17 no.4
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• pp.143-156
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• 1974

The purpose of this experiment was to study one side of germination physiology based on that protein profiles and protease relating to protein metabolism, that peroxidase, catalase, $\alpha$-amylase, $\beta$-amylase, and malate dehydrogenase involved in the carbohydrate metabolism of seed germination. All these experiments were divided into the two groups with and without acetone treatment, and were carried out. The protein bands of each germinating stage between the groups treated with and without acetone showed certain basic pattern in polyacrylamide gel disc electrophoresis. However, there was a little difference in the number of protein band, optical density, and migration velocity between two groups. The isozyme bands of peroxidase, and catalase between two groups in polyacrylamide gel disc electrophoresis did not show the numeral difference, but the optical density of certain germinating stage treated with acetone was higher than the group untreated with it and it showed their enzyme activity. The $\alpha$-amylase and $\beta$-amylase activities which involved in starch metabolism of seed germination were higher in the treated group than the other. On one hand, the protease activity of hydrolase occurred in the seeds for germination was also higher, more or less in the treated group than in the other. The isozyme band pattern of malate dehydrogenase in TCA cycle of energy metabolism pathway was very different between two groups growing for 72 hours with and without acetone treatment in cellulose acetate electrophoresis. It indicated that two isozyme bands of malate dehydrogenase was high. Consequently these experimental results mentioned above indicated that acetone treatment before sowing had an effect on dissolving certain complexed lipid substance involved in the seed coats, the activity of carbohydrate hydrolase increased with water absorption which was most comfortable in its germination, dissolved glycerin and fatty acid became certain energy source, and they stimulated the acceleration of respiration metabolism.

• Journal of Korean Society of Forest Science
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• v.66 no.1
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• pp.79-81
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• 1984

Protein profiles of healthy and witches' broom (mycoplasma) infected jujube plants (Zizyphus jujuba) were investigated by 2-30% linear gradient polyacrylamide gel electrophoresis. Distinct differences in band patterns between healthy and infected samples were observed. Gels from samples of healthy leaves showed a characteristic protein band in the 50kd-range, which was not detected in infected leaves. Band with 25kd was more distinct in the infected leaves, whereas band with 198kd was more apparent in the healthy leaves. Healthy-looking leaves in the infected samples demonstrated the characteristic band of 50kd with less intensity showing intermediate pattern between healthy leaves and infected leaves. In contrast with leaf samples, gels from infected stem samples showed a characteristic band in the 335kd-range which was absent in healthy stem samples.

• Korean Journal of Ichthyology
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• v.19 no.3
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• pp.193-200
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• 2007

The samples of cultured flounder (Paralichtys olivaceus) were collected from Tongyeong and Yosu in Korea and Nagasaki in Japan and processed to analyses of body and serum. Body size and weight were not significantly different between Korea and Japan produced. But the body weight of average size of flounder produced from Nagasaki was the highest from the samples. The average of fish body was not significantly different from the samples. The water content of fish body was lower in samples from Nagasaki and fat content was higher in the samples from Nagasaki and followed by Yosu and Tongyeong. The percentage of body water, fat and protein contents was Nagasaki, Yosu and Tongyeong in the order of high values. Japanese flounder showed relatively high content of body fat and muscle. The cholesterol of serum was highest in the samples of Yosu. The triglyceride of serum was highest in the samples of Nagasaki. Lipoprotein was highest in the samples of Nagasaki and enriched fat content in LDL fraction.

• Parasites, Hosts and Diseases
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• v.29 no.2
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• pp.113-120
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• 1991

When the component proteins in crude saline extract of 13-week old adult Paragonimus westermani were observed by non-denaturing discontinuous-polyacrylamide gel electrophoresis (Disc-PAGE), 8 distinct bands were clearly recognized. Molecular weight (MW) of each band protein, numbered in sequence from cathodal side which appeared in 10% separating gel, was measured first by Ferguson plot utilizing different gel concentrations from 10% to 4.5%. MW of band 1 Protein (known as egg Protein) was 440 kDa. And MW of other band Proteins were: 386 kDa in band 2, 17.4 kDa in band 3, 17kDa in band 4, 14.3 kDa in band 5, 46 kDa in band 6, 38 kDa in band 7 and 23 kDa in band 8. When the proteins in the crude extract were separated into fractions by molecular sieve chromatography through 1.6 (Φ)×70cm sired Sephacryl 5-300 Superane column and revisualized by Disc-PAGE in 8% gel, the sequence of fluted proteins was band 1, band 2, band 6, band 7 and bands 3,4,5 and 8. This elusion profile confirmed MW of each band protein in the crude extract as measured by Ferguson plot.