• Title/Summary/Keyword: dipping method

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Breeding of Resistant Cabbage 'CT-171' to Fusarium Wilt (시들음병 저항성 양배추 품종 'CT-171' 육성)

  • Song, Jun-Ho;Kim, Gi-Jun;Kim, Kyoung-Cheol;Han, Tae-Ho
    • Horticultural Science & Technology
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    • v.32 no.1
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    • pp.129-132
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    • 2014
  • A new resistant cabbage variety 'CT-171' to Fusarium wilt was bred by crossing $A337MSBC_5$ with resistance to the disease and 397-$G_6$ with good density and color. 'CT-171' was selected after combining ability, seed gathering and regional adaptability test in 2008. For breeding of resistant varieties, we investigated the development of Fusarium wilt on cabbage seedlings inoculated with Fusariumoxysporum f. sp. conglutinans by root dipping inoculation method. As a result, 'CT-171' showed higher resistance to Fusarium wilt than 'Asiaball' used as control. The maturity of new variety was 58 days and was faster than control and well suited for autumn cultivation because of cold resistance. The anthocyanin pigment of plant was not revealed. The weight, height and width of head were 1.5 kg, 14 cm and 15 cm, respectively and the core size was 5.7 cm and stable in various cultivation environments. 'CT-171' which showed good agricultural character and resistance to Fusarium wilt filed for variety protect right in Korea Seed & Variety Service on February 2013. The new variety will be appropriate for export and domestic consumption.

Comparison of Insecticidal Activity and Feeding Behavior of Nilaparvata lugens by Root Uptake Times against Fenobucarb and Imidacloprid (Fenobucarb와 Imidacloprid의 벼 뿌리 침지시간에 따른 벼멸구의 살충활성과 섭식행동비교)

  • Yang, Jeong-Oh;Cho, Sun-Ran;Kwon, Yun-Hee;Yoon, Chang-Mann;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.14 no.2
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    • pp.175-182
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    • 2010
  • Two insecticides, fenobucarb (36, 12 ppm) and imidacloprid (0.7, 0.4 ppm) were treated on rice seedling roots by root uptake method with different dipping time (1, 4, 8, 12, 16, 20, 24 hrs). This study was performed to elucidate the correlation between insecticidal activity and feeding behavior of Nilaparvata lugens using EPG (electrical penetration graph). EPG waveforms are recorded for 4 hours and classified into six waveforms. In the correlation between root uptake and corrected mortality, both insecticides showed higher corrected mortality as higher doses and as longer root uptake times. In the analysis of waveforms, N. lugens showed longer nonprobe time but shorter phloem feeding time at a higher dose. It was also showed the same result as longer the root uptake times. Therefore, it showed the correlation between insecticidal activity by root uptake time and both EPG waveforms (non-probing time and phloem feeding time).

Insecticide Susceptibility in the Different Larva of Tobacco Cutworm, Spodoptera litural Fabricius (Lepidoptera: Noctuidae) Collected in the Soybean Fields of Milyang, Korea (밀양산 콩포장 담배거세미나방 유충의 약제에 대한 감수성)

  • 배순도;최병렬;송유한;김현주
    • Korean journal of applied entomology
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    • v.42 no.3
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    • pp.225-231
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    • 2003
  • The susceptibility of the different larval stages of Spodoptera litura to nine insecticides was evaluated using the perilla leaf-dipping method. Median lethal concentration ($LC_{50}$) was increased with larval development in the range of 0.5 ppm to 5.6 ppm, 9.9 ppm to 27.9 ppm, 9.6 ppm to 125.1 ppm and 24.3 ppm to 546.6 ppm in the 1st, 2nd, 3rd and 4th instar, respectively. The tolerance ratio (TR), which is the TR of 90 percent lethal concentration (LC/ sub 90/) to the recommended concentration, was 0.04 to 0.8 in the 1 st, 0.2 to 7.5 in the 2nd, 0.7 to 115.3 in the 3rd and 1.2 to 485.4 in the 4th instars. Lower D$LC_{50}$ and DTR, which is the difference between the $LC_{50}$ and the TR of 4th and other instars, respectively, were observed in chlorfenapyr, chlorpyrifos and EPN while higher ones were lufenuron, chlorfluazuron and teflubenzuron. These results mean that insecticides with lower D$LC_{50}$ and DTR are effective in controlling larva of S. litura collected in Milyang, Korea.

Selective Toxicity of Insecticides to Plant-and Leaf-hoppers (멸구$\cdot$매미충의 종류에 따른 살충제의 선택독성)

  • Choi S. Y.;Lee H. R.
    • Korean journal of applied entomology
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    • v.15 no.1 s.26
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    • pp.1-6
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    • 1976
  • his study was conducted to investigate selective toxicity of several organophosphorous and carbamic insecticides against the small brown planthopper (Laodelphax striatellus), the white-backed planthopper (Sogatella furcifera), the brown planthopper (Nilaparvata lugensEugens), and the green rice leafhopper (Nephotetix cincticeps). The test insecticides were the organophosphoric insecticides, Diazinon$(34\%\; Ec.,\; 3\%\; G.),$ $MEP(Sumithion 50\%\; Ec.)$, and PAP(Elsan\; 47.5\%\; Ec.)$ and the carbamic insecticides. MPMC $(Meobal\; 50\%\;WP.)$, $BPMC(Bassa\; 50\%\; Ec.)$, and carbofuran $(Curaterr; 3\%\; G.)$. Toxicitiy for emulsion concentrates and wettable powders were evaluated by dipping method of rice seedlings in the laboratory and that for granulated insecticides evaluated by submerged application with potted plants in the greenhouse. In the test of contact toxicity by seedling dips, Diazinon MEP and PAP showed significantly low toxicity to the brown planthopper and MEP to the green rice leafhopper. In the test of submerged application, Carbofuran showed almost equal toxicity to the four species of the hoppers, but Diazinon was greatly less toxic to the brown planthopper. In general. the organophosphorous insecticides tested seemed to be selective in toxicity to the species of the hoppers, while the carbamic insecticides being less selective. The carbamic insecticides such as MPMC, BPMC and Carbofuran were almost equal in toxicity to the four species of the hoppers tested.

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In vitro Uptake and Accumulation of Purified Storage Proteins into Fat Body Cells from Huphantria cunea Drury (생체밖에서 미국흰불나방 지방세포에 의한 저장단백질의 흡수와 축적에 관하여)

  • Lee, Bong-Hee;Kim, Kwan-Seon;Moon, Myung-Jin;Kim, Woo-Kap
    • Applied Microscopy
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    • v.18 no.2
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    • pp.102-118
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    • 1988
  • This study was carried out to examine in vitro first whether the storage proteins, which the fat bodies of last larvae from Hyphantria cunea secrete into haemolymph, can be uptaked by the fat body cells of prepupa and then how the uptaked storage proteins can be accumulated in the fat body cells, if uptaken. The fat bodies which had been isolated from last instar larvae were cultured in 1 ml of Grace's insect medium containing $50{\mu}l$ of $^{3}H$-leucine (5.0 mCi/mol, Dupont) at $28{\pm}2^{\circ}C$ for 6 hrs. After the homogenates of the cultured fat bodies were centrifuged at 10,000 rpm for 10 minutes, the proteins included in the supernatant were separated by polyacrylamide gel electrophoreses (non-SDS, 6%). The next treatment of the electrophoresed gel was followed by rinsing. A storage protein band of several bands in the rinsed gel was sliced off. With elution of sliced storage protein bands in Tris-glycine buffer, the purification of radioactive storage proteins from fat bodies was finished. After the purified radioactive storage proteins were added in Grace's insect midis containing fat bodies of the prepupae, they were cultured for the randomly following minutes given as 3, 5, 7, 10, 15, 20 and 30 and for the randomly following hours given as 1, 2, 3 and 4 respectively. The double fixations of the cultured fat bodies in aldehyde and $OsO_4$, were followed by preparation of ultrathin sections from Epon-Araldite blocks through dehydration and embedding. The electron microscope autoradiographic treatment of all prepared sections were performed by the dipping method (Kim et al., 1987). The finally prepared specimens were examined with electron microscope. The fat body cells of the prepupa could be found to uptake the storage preteins of the last instar larvae, which were included in the culture medium, mostly by formation of coated vesicles. The in vitro uptake of the storage proteins actively occurred by 30 minutes after the addition of purified storage proteins in the culture medium. After culture for 7 minutes with the storage proteins, the uptaked radioactive storage proteins labelled a number of lysosomal granules. After culture for 20 minutes with the storage proteins, the radioactive storage proteins were finally incorporated and accumulated in lipid droplets and protein granules. The frequency in the fat body cell of radiolabelled lipid droplets occurs approximately 60%, while the frequency, in which the radiolabelled protein granules occurs in a fat body cell, is approximately 40%.

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Effects of Geological Structures on Slope Stability : An Example from the Northwestern Part of Daegu, Korea (퇴적암 내의 지질구조가 비탈면 안정성에 미치는 영향 : 대구 북서부 지역의 예)

  • Ko, Kyoung-Tae;Choi, Jin-Hyuck;Kim, Young-Seog
    • The Journal of Engineering Geology
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    • v.22 no.1
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    • pp.1-13
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    • 2012
  • The purpose of this work is to gain a better understanding of the interrelationships between geological structures and slope failure in sedimentary rocks. In the studied slopes, construction-related slope failure could only be observed on the south-dipping slopes. This indicates that slope stability may be dependent on the angular relationships between the dip direction of bedding and the orientation of the slope. Slope failure continued, post-construction, around large fault zones in the studied outcrop; these fault damage zones are, however, not easily recognized in the field. Here we suggest a new method that uses accumulated fracture density to precisely identify fault damage zones. Multiple-faced slopes are now increasingly being exposed during large-scale construction projects in South Korea. This multiple-faced slope analysis indicates that the stability of a slope should be evaluated by identifying domains, through the analysis of possible slopes and their angular relationships with bedding and other discontinuities, prior to construction. Therefore, careful consideration of geological structures such as bedding and other discontinuities, and their angular relationships during the design of cuttings through sedimentary rocks, will increase the efficiency of construction and enable the safe construction of more stable slopes that will retain their stability after construction.

Chemical Characterization and Insecticidal Activity of Rubus coreanus Miquel Extracts (Leaves, Fruits and Stems) against Three Agricultural Insect Pests, Myzus persicae, Plutella xylostella and Spodoptera litura (특용작물:복분자의 화학적 특성 및 복분자 부위별 (잎, 열매, 줄기) 추출물을 이용한 농업해충 복숭아혹진딧물, 배추좀나방 및 담배거세미나방에 대한 살충효과 연구)

  • Lee, Hee-Kwon;Lee, Hoi-Seon
    • Korean Journal of Organic Agriculture
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    • v.24 no.4
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    • pp.849-857
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    • 2016
  • In the growth of the Rubus coreanus Miquel fruits, the unripened (10 days) and ripened (25 days) fruits after flowering were harvested. The chemical characteristics of different maturational stages of the unripened and ripened fruits were investigated. Total amount of phenolic compounds was 4.00-7.56% in the unripened fruits and 3.78-5.57% in the ripened fruits, respectively. Furthermore, total amounts of organic acids such as malic, citric, succinic, and oxalic acids were 16.40 mg/100 g in the unripened fruits and 28.82 mg/100 g in the ripened fruits, respectively. In organic acids of the unripened and the ripened fruits, citric acid (8.76-15.47 mg/100 g) was the highest amount among other organic acids. Soluble sugars were significantly increased from 11.07 to 21.54% in the unripened and ripened fruits. Therefore, ripened fruits had the high levels of phenolic compounds, organic acids and soluble sugars. For the biological studies of R. coreanus, methanol extracts of R. coreanus leaves, fruits, and stems were evaluated for their insecticidal activities against Myzus persicae (Hemiptera: Aphididae), Plutella xylostella (Lepioptera: Plutellidae) and Spodoptera litura (Lepioptera: Noctuidae) by leaf dipping method. From these results, the extract of R. coreanus leaves revealed potent insecticidal activity against P. xylostella. However, the methanol extracts of R. coreanus fruits and stems had no any insecticidal activity against M. persicae, P. xylostella and S. litura. The R. coreanus leaves have promising potential as new insecticidal agent against P. xylostella.

Eco-friendly Materials Selection and Control timing to Eurytoma maslovskii in Japanese Apricot (매실 복숭아씨살이좀벌에 대한 유기농업자재 선정 및 방제적기)

  • Cho, Young-Sik;Song, Jang-Hoon;Choi, Jin-Ho;Choi, Jang-Jeon;Kim, Myung-Su
    • Korean Journal of Organic Agriculture
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    • v.24 no.1
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    • pp.123-130
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    • 2016
  • The mortality was tested to organic control materials to the E. maslovskii adults by the dipping method in laboratory. As the result, in 2014, the extracts of Neem I was not shown control effect by 10.2% control efficacy to E. maslovskii adults in laboratory. The extract of Sophora flavescens roots was shown 84.7% mortality. And other 5 materials were shown 100% insecticidal activity to the adults in laboratory. I n 2015, Plant extract+pyrolignous acid Matrine 0.45% and Plant extract Matrine 0.5+paraffin oil 8% were shown 100% and 94.6% control efficacy, respectively. In the Japanese apricot orchards, the fruit damage rate was low on spray at 21th April in Daap, Gwangyang city, and 8th May Hwangjeon, Suncheon city in Jeonnam province in 2014. The fruit damage rate differed from spray timing and materials, but it was considered that the control of E. maslovskii should be in late April to early May. Otherwise, in 2015, the fruit damage was significantly low in late April spray in Daap, but no significant was in between one spray at 21th, 27th April and 2 spray that days. Meanwhile, the control effect was better 2 times spray of the days in Hwangjeon, Suncheon city in Jeonnam province. Therefore, in organic Japanese apricot, the use of insecticidal materials recommended to control over twice spay in from meddle to late April.

A Study on the Structural Deformations in the Sedimentary Layer Resulted from Magma Intrusion (마그마관입에 의한 상부퇴적층의 변형에 관한연구)

  • Min, Kyung Duck;Kim, Won Young
    • Economic and Environmental Geology
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    • v.10 no.1
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    • pp.37-48
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    • 1977
  • The earth's crust is unceasingly undergoing deformations because of the forces acting upon it. The relationship between the tectonic forces and the resulting deformations are found from the states of stresses in the earth's crust induced by these forces. The study has been attempted to analyze the deformations of the overlying sedimentary layers, which are deformed by the magma intrusion along its lower boundary. The elastic model is constructed to analyze the geologic structures, by means of the theory of elasticity, and then the appropriate boundary conditions are given. The solution of the Airy stress function which satisfies the given boundary conditions is derived from the analytic method. The internal stress distributions of the deformed elastic model layer are portrayed by principal stress trajetories, and then the corresponding potential faults and joints systems are predicted from the Coulomb-Mohr failure criterion. The internal displacement distributions are shown by the calculated displacement components vectors, namely horizontal, vertical and net components. Results of the numerical calculations show the developments of some geologic structures as follows; (1) one set of shear joints and or two sets of shear joints which are oppisite directions, and one set of extension joints parallel to the ${\sigma}_1$ direction, (2) one set of high angle thrusts and normal faults, (3) symmetric fold; both limbs are dipping in opposite direction with low angle. The field work at the Wall-A San area, located near Jinju City, in southern Korea, had accomplished to compare the field structures with the predicted ones. The results of the comparison exhibits the developments of joint and fault systems satisfactorily consistent with each others. But the area does not show any type of folding, in spite of the intrusion of a granodiorite massif, this fact is one of the important features of the whole Kyungsang sedimentary basins of Mesozoic age distributed at the south-eastern parts of Korea. For this reason, it is thought that the magma intrusion had occurred with extremly low pressure. The geologic structures have been modified by the erosion and weathering throughout the geologic time, and the conditions of the sedimentary layers (width, thickness and radius of magma) are not the same as before, being intruded by the magma. To enlighten this, it is preferable to study these geologic structures with analyses of various types of rheological models.

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Effect of On-site Postharvest Hot Water Treatment on Storage Quality of Commercial Greenhouse Satsuma Mandarin (현장 열수처리에 따른 온실재배 온주감귤 상품의 저장 중 품질특성 변화)

  • Lee, Hyun-Hee;Hong, Seok-In;Son, Seok-Min;Kim, Dong-Man
    • Korean Journal of Food Science and Technology
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    • v.43 no.5
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    • pp.577-582
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    • 2011
  • Greenhouse satsuma mandarins (Citrus unshiu Marc., cv. Gungchun) of an early harvesting cultivar were treated by hot water showering at 65$^{\circ}C$ for 10 s at a commercial scale in a packing house and then stored at 5$^{\circ}C$ for 3 weeks and subsequently at 18$^{\circ}C$ for 1 week (simulated shelf-life) to examine the potential use of hot water treatment (HWT) as an environmentally benign method to maintain mandarin quality characteristics during postharvest storage and sale. The respiration rate just after heat treatment or during storage was at a similar level in both the treated and untreated fruit. HWT also had no detrimental effects on quality attributes including pH, titratable acidity, soluble solids content, weight loss, firmness, and peel color. The development of stem-end rot, mold decay, and black rot was lower in the heat-treated fruit compared to those in the untreated control. A sensory evaluation showed that HWT markedly improved fruit appearance, making the fruit cleaner and glossier. The results suggested that HWT can be applied to satsuma mandarin as an effective pretreatment to maintain postharvest quality during storage and marketing.