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Monitoring and Risk Assessment of Heavy Metals in Edible Mushrooms (국내 유통 버섯 중 중금속 함량 조사 및 위해성 평가)

  • Kim, Ji-Young;Yoo, Ji-Hyock;Lee, Ji-Ho;Kim, Min-Ji;Kang, Dae-Won;Ko, Hyeon-Seok;Hong, Su-Myeong;Im, Geon-Jae;Kim, Doo-Ho;Jung, Goo-Bok;Kim, Won-Il
    • Korean Journal of Environmental Agriculture
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    • v.31 no.1
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    • pp.37-44
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    • 2012
  • BACKGROUND: Many edible mushrooms are known to accumulate high levels of heavy metals. This research was focused on health risk assessment to investigate the mushrooms in Korea, arsenic (As), cadmium (Cd), lead (Pb) and mercury (Hg) contaminations in edible mushrooms in cultivated areas were investigated, and health risk was assessed through dietary intake of mushrooms. METHODS AND RESULTS: The heavy metals in mushrooms were analyzed by ICP/MS after acid digestion. Probabilistic health risk were estimated by Monte-Carlo simulation techniques. The average contents of As, Cd, Pb, and Hg were $0.035{\pm}0.042$ mg/kg, $0.017{\pm}0.020$ mg/kg, $0.043{\pm}0.013$ mg/kg, and $0.004{\pm}0.004$ mg/kg, respectively. The results showed that contents of Cd and Pb did not exceed maximum residual levels established by European Uion regulation (Cd 0.20 mg/kg and Pb 0.30 mg/kg). For health risk assessment, estimated intakes in all age populations did not exceed the provisional tolerable daily intake of As and Hg, provisional tolerable monthly intake of Cd, provisional tolerable weekly intake of Pb. The Hazard Index (HI) were ranged from $0.03{\times}10^{-4}{\sim}0.01{\times}10^{-3}$ for As, $0.02{\times}10^{-3}{\sim}0.81{\times}10^{-3}$ for Cd, $0.06{\times}10^{-3}{\sim}0.38{\times}10^{-3}$ for Pb, and $0.08{\times}10^{-4}{\sim}0.14{\times}10^{-3}$ for Hg at general population. CONCLUSION: The HI from the ratio analysis between daily exposure and safety level values was less than 1.0. This results demonstrated that human exposure to heavy metals through dietary intake of mushrooms might not cause adverse effect.

Detection of Metagonimus sp. (Family Heterophyidae) Metacercariae from Freshwater Fishes in the Vicinity of Tong River, Kangwon Province, Korea (동강유역 담수어에 기생하는 흡충(Metagonimus sp.)의 피낭유출 감염 조사)

  • 전계식;김태선
    • Korean Journal of Environmental Biology
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    • v.17 no.2
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    • pp.169-173
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    • 1999
  • The present study was undertaken to survey the infection status of Metagonimus sp. metacercariae in freshwater fishes from Tong river located in Kangwon Province, a total of 178 freshwater fishes of 11 species were collected by a fish net and traps from the end of September to early October 1998. They were brought to the laboratory and examined under a stereomicroscope after artificial digestion with pepsin-HCI solution for one hour. Ten out of eleven species of freshwater fishes examined were found to contain metacercariae of Metagonimus sp. The most frequently infected fish was Liobagrus andersoni Regan 91.7% (11 of 12 fishes), followed by Gobiobotia breviburba Mori 75.0% (6 of 8 fishes), Zacco temmincki 66.2% (49 of 74 fishes), Cobitis koreensis Kim 50.0% (1 of 2 fishes), Microphysogobio longidorsails Mori 42.9% (9 of 21 fishes), Cobitis rotundicudata Wakiya et Mori 33.3% (2 of 6 fishes), Hemibarbus longirostris 33.3% (1 of 3 fishes), Pungtungia tenuicorpus 30.0% (3 of 10 fishes), Coreoleuciscus splenddus Mori 28.6% (4 of 14 fishes), Coreoperca herri Herzeanstein 11.1% (1 of 9 fishes) respectively Metagonimus metacercariae was not found from Moroco kumgangensis Uchida of freshwater fish. The total number of Metagonimus sp. metacercariae was 81, 17, 216, 1, 14, 2, 1, 4, 10, 1, respectively. Infection rates of Metagonimus sp. in the five districts was shown as follows : Chongson-up Kyulam-ri 50.0% (12 of 24 fishes), Chongson-up Kasu-ri 34.0% (17 of 50 fishes), Shindong-up Unchi-ri 56.0% (14 of 25 fishes), Shingdong-up Kosong-ri 57.1% (20 of 35 fishes), Shingdong-up Duckchon-ri 54.5% (24 of 44 fishes) respectively. Infection rate of freshwater fish was 48.9% (87 of 178 fishes) and from the results obtained in this survey, it was confirmed that freshwater fishes from Tong river was infected with metacercariae of Metagonimus sp.

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Prevention of Discoloration and Storage Stability in Canned Ark Shell (새고막 통조림 변색방지 및 저장중 품질변화)

  • 배태진;김귀식
    • The Korean Journal of Food And Nutrition
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    • v.11 no.2
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    • pp.243-248
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    • 1998
  • Ark shell was known as shellfish that had hemoglobin as blood pigment and the action of mecidine, was consumed the great part of it as raw material, though it was produced about 13,000 M/T per year. Ark shell was processed the infinitesimal quantity as conned product, bout canned ark shell had problem that occurrenced discoloration after heat treatment during processing and storage. This discoloration mechanism during processing and storage was not cleared. This study was carried out to understand characteristics of the hemoglobin as blood pigment and carotenoid as meat pigment in ark shell and management of proper processing conditions for prevention of oxidation and discoloration by thermal treatment. When treated by digestion of 0.1% BHA, 0.1% Tenox-II, 0.5% Na2EDTA, 0.05% NDGA and 3% salt soln., 0.1% BHA solution was most suitable for stability of carotenoid that the retention ratio of carotenoids were 63.1% after heating to 116$^{\circ}C$ for 120 minutes. In preparation of canned ark shell and storage at 37$\pm$1$^{\circ}C$ for 60 days, the chemical composition, pH and salinity ere stable. And contents of total carotenoid were decreased slightly from 0.83mg% to 0.727mg%. The viable cell count were 6.92$\times$103 cfu/ml at raw ark shell, after processed and storage were not detected. The predominant amino acids in the raw ark shell were glutamic acid(19.7%), arginine(16.0%), glycine(12.6%), alanine(12.2%) and aspartic acid(7.6%). When 60 days stored, the contents of amino acid were stable. And the predominant nuclotide and their related compounds in the raw ark shell were hypoxanthine(2.14$\mu$mol/g), IMP(1.94$\mu$mol/g) and ATP(0.87$\mu$mol/g), and storage at 37$\pm$1$^{\circ}C$ for 60 days, the quantity order were same as raw material.

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Characteristics of Heavy Metal Contamination in Residual Mine Tailings Near Abandoned Metalliferous Mines in Korea (국내 폐금속광산 주변 잔류광미의 중금속 오염특성)

  • Jung, Goo-Bok;Kim, Won-Il;Lee, Jong-Sik;Lee, Jae-Saeng;Park, Chan-Won;Koh, Mun-Hwan
    • Korean Journal of Environmental Agriculture
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    • v.24 no.3
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    • pp.222-231
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    • 2005
  • Most of the tailings have been left without any management in abandoned metalliferous mines and have become the main source of heavy metal contamination of agricultural soils and crops in the these areas. To compare of environmental assessment of heavy metals in tailings derived from various 25-metalliferous mines in Korea, 3 different analysis methods such as water soluble, 0.1 M-HCl extractable, and total acid digestion method (aqua regia) were used. The chemical composition of water soluble in mine tailing were in the order ${SO_4}^{2-}>Ca^{2+}>Mn^{2+},\;Na^+,\;Al^{3+}>Mg^{2+},\;Fe^{3+}>Cl^-$. Specially, pH, EC, ${SO_4}^{2-},\;and\;Ca^{2+}$ concentrations in tailing varied considerably among the different mines. The average total concentrations of Cd, Cu, Pb, Zn, and As in tailing were 31.8, 708, 4,961, 2,275 and 3,235 mg/kg, respectively. Specially, the contents of Cd, Zn and As were higher than those of countermeasure values for soil contamination (Cd : 4, Zn : 700 and As : 15 mg/kg in soil) by Soil Environmental Conservation Act in Korea. The rates of water soluble heavy metals to total contents in tailings were in the order Cd > Zn > Cu > Pb > As. The rates of 0.1M-HCl extractable Cd, Cu, Pb, Zn, and As (1M-HCl) to total content were 17.4, 10.2, 6.5, 6.8 and 11.4% respectively. The enrichment factor of heavy metals in tailings were in the order As > Pb > Cd > Cu > Zn. The pollution index in tailing Au-Ag mine tailing were higher than those of other mine tailing. As a results of enrichment factor and pollution index for heavy metal contaminations in mine tailing of metalliferous mines, the main contaminants are mine waste materials including tailings.

Studies on the phrases of Yellow Emperor's internal classic(黃帝內經) for the physiology on the spleen and stomach (비위생리(脾胃生理)에 수용(授用)되는 황제내경(黃帝內經) 어구(語句)에 관(關)한 연구(硏究))

  • Won, Jin-Hui
    • The Journal of Korean Medicine
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    • v.16 no.2 s.30
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    • pp.453-489
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    • 1995
  • The research of the phrases related with physiology of stomach and spleen in the contents of Huang Di Nei Jing(黃帝內經) known as the Bible of oriental medicine will make a contribution to a deep understanding of disease of stomach and spleen and a proper clinical diagnosis and treatment of them. In this research of the most appropriate glosses recorded nine kinds of representative medical books including Huang Di Nei Jing Somoon(黃帝內經素問) of Wang Bing(王氷) were picked out: The summaries of the selected contents are as follows: 1. The word 'saliva(涎)' in 'the spleen controls saliva(脾爲涎)' can be viewed as a generic term referring to oral cavity secretion gland as well as the secretion fluid of salivary gland. 2. The phases 'a large reservoir(太倉)', barn organs', 'a reserboir of food stuff', 'a stomach as the market(胃爲之市)', etc mean the function of stomach to receive food(胃主受納). 3. The phase 'generation of five tastes(五味出焉)' means both 'the function of stomach to transform food into chyme(胃主腐熟)' and 'the channelling function of spleen.(脾主運化)' 4. The flowing of the food-Qi(食氣) into stomach brings about spreading Jung(精) into liver and then percolating Jung(精) flow into channel. The channel-Qi(脈氣) flows into lung through channel. As a result, all kinds of channels gather together in lung and Jung(精) is sent into skin and hair. The assembly of Jung(精) with skins and channels moves Qi(氣) into fu-organ and so jung(精) and mental activity(神明) in fu-organ(府) come to be in four organs(四臟). Then if Qi(氣) comes back to power balance unit(權衡) being in the state of equilibrium(權衡以平), the hole of Qi(氣口) comes to determine the matter of life and death through achieving Chun-quan-chi(-寸-關-尺). The above mentioned phrases means the digestion, asorption and transmission of food. When food is taken in stomach, Jung-Qi(精氣) comes to be over flowed upward into spleen, back into lung, finally downward into bladders through water-conduit(水道) controlled by lung. When water- Jung(水精) radiates into whole body with channels of five organs(五臟), both of them fit together with and yin-yang(陰-陽). Therefore, the grasping of the rise and decline of yin-yang(陰C-陽) is necessary to consult patients. The above mentioned phrases is properly viewed to designate the asorption, transmission and excretion of food. 5. Spleen controls flesh(脾之合肉也), the state of spleen is known by human lips, and what this means is that liver plays functions of spread and expansion(肝主疏泄). 6. The phrase 'Jung Jung'((中精)) in 'gallbladder dominates Jung jung(膽主中精)', which in one of the specific expression of 'liver plays functions of spread and expansion(肝主疏泄). 7. It is right that the phase 'The eleven organs in all are determined by gallbladder'(凡十,一臟取決於膽也) is correctly paraphrased as 'only one of ten organs, spleen, is determined by gallbladder'.(凡十,一臟取決於膽也), 8. The small intestine is an organ. which receives the materials digested and sends them out. This means that the function of transforming materials(化物) factually refers to that of separating clearity and blur(泌別淸濁). And it is also thought to have the function of ascending clearity and descending blur(升淸降濁), 9. A large intestine is a transmitting organ(傳導之官) from which a change comes out(變化出焉). the phrase 'change'(變化) in this sentence means both the intake of water and nutrition and the formation procedure of stool through excretion of mucocele.

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Effect of Environmental Factors on the Growth of Rabbit Oral Keratinocytes (토끼 구강점막 상피세포 성장에 미치는 환경인자의 영향)

  • Yoon, Moon-Young;Park, Hee-Jung;Lee, Doo-Hoon;Jang, In-Keun;Park, Jung-Keug;Kim, Woo-Sik
    • Korean Chemical Engineering Research
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    • v.43 no.1
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    • pp.103-109
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    • 2005
  • Isolation and primary culture technique of rabbit oral keratinocytes, and the study for effect of environmental factors on the cell growth were carried out in T75-flask. $1.92{\pm}0.59{\times}10^6$ viable cells were isolated by trypsin enzymatic digestion method from $0.25cm^2$ biopsy of rabbit oral mucosa. Primary culture with 10 mL of K-SFM containing 50 mg/L BPE, $5.0{\mu}g/L$ EGF and 0.15 mM $Ca^{2+}$ showed confluence after 8 days and doubling time was 2.54 days. Effect of medium types, medium volume and supplement types on the cell growth was investigated after the cultured keratinocytes had been harvested from primary confluence. Serum addition showed adverse effect and the increase of serum concentration didn't have an effect on the cell growth. The increase of medium volume decreased the cell growth. The increase of calcium concentration increased the cell growth and 2.0 mM was optimum value. In conclusion, when rabbit oral keratinocytes was cultured in T75-flask, the most effective conditions was to use 10 mL of K-SFM containing 50 mg/L BPE, $5.0{\mu}g/L$ EGF and 2.0 mM $Ca^{2+}$, and doubling time was 1.32 days. This study can provide the useful informations to develop a process and design a bioreactor for the culture of keratinocytes in human body like skin and cornea, as well as mucosa.

In situ posture of anterior body of Metagonimus yokogawai in experimentally infected dog (개의 실험적 요꼬가와흡충증에서 충체의 자세)

  • 장영기;강신영
    • Parasites, Hosts and Diseases
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    • v.23 no.2
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    • pp.203-213
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    • 1985
  • The in situ posture of anterior body of Metagonimus yokogawai was observed in experimental metagonimiasis of dog. The metacercariae were collected from naturally infected sweetfish by peptic digestion; a total of 8 dogs was orally infected with 10,000 metacercariae respectively. Two dogs were killed on 3 days, 9 days, 4 weeks and 10 weeks after the infection. The postures of worms in histological section of small intestine and of whole worms collected from the fixed intestinal mucosa were examined by light and scanning electron microscope. The results were summarized as follows: 1. The recovery rates of worms were 42.6% on 3 days, 55.0% on 9 days, 33.2% on 4 weeks and 9. 8% on 10 weeks after the infection respectively. 2. In histological sections of small intestine, most of worms were found at intervillous spaces as ovoid sections of posterior body. However, many worms, especially in 3 day-old worms, revealed protruded anterior body in glandular lumens of crypt. Some sections of anterior body were bifurcated or sacculated. 3. The worms collected from fixed intestinal mucosa under dissecting microscope exhibited a variety of postures. Many worms showed flat shapes with a concavity or curvatures. However, in many worms, the anterior body made a single or multiple protrusions. 4. By SEM observation of protruded anterior body, a longitudinal groove was found. 5. The frequency of worms with protruded anterior body decreased in 9 days, 4 weeks and 10 weeks than in 3 day-old worms. The above findings indicated that the anterior body of juvenile and adult M. yokogawai protruded to lumens of glandular crypt by folding their lateral portions to make a reversible tubelike structure. Frequent multiple protrusions were considered to be made to adapt the microniche of glandular crypts of dog intestine.

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Relationship of Dental Health Assessment to the Number of Existing Permanent Tooth in Senior Citizens Visited a Dental Hospital or Clinic from Some Regions (치과병·의원에 내원한 65세 이상 노인의 치아건강사정에 따른 현존영구치아수)

  • Kim, Mi-Jeong;Lee, Hae-Kyung
    • Journal of dental hygiene science
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    • v.7 no.3
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    • pp.161-166
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    • 2007
  • Relationship of Dental Health Assessment to the Number of Existing Permanent Tooth in Senior Citizens visited a dental hospital or clinic from Some Regions. The purpose of this study was to examine the relationship of the dental health assessment of elderly people who were at the age of 65 and up to the number of existing permanent tooth in an effort to lay the groundwork for pinpointing problems with their oral status and developing programs geared toward improving their dental health and quality of life. The findings of the study were as follows: 1. Regarding self-perceived dental health, the largest group of the senior citizens that accounted for 57.9 percent didn't find themselves to be in good dental health. The second largest group that represented 32.5 percent thought their dental health was at an ordinary level, and the third greatest number of them that stood at 9.5 percent considered themselves to be in good dental health. Their self-perceived dental health status was statistically significantly different according to education and monthly income, and whether they had a job or not made a statistically significant difference to that as well(p < .01, p < .001). 2. In terms of taste appreciation, 58.7 percent, the largest group, didn't appreciate one or two kinds of taste, and their taste appreciation was statistically significantly different according to education and monthly income(p < .05, p < .01). 3. As for chewing ability, 20.6 percent were able to chew well. In regard to digestion, 69.0 percent had an ordinary level of digestive power. 4. Concerning the number of existing permanent tooth, 38.9 percent, the greatest percentage, had ten or less permanent teeth. By education, the possession of 21or more permanent teeth(40.0%) was most common among those who received high-school or higher education. The greatest group of the senior citizens with a job had 11 to 20 permanent teeth(41.7%), and the largest number of the elderly people whose monthly income was 500 thousand won or more were in possession of 11 to 20 permanent teeth(41.7%). The number of existing permanent tooth statistically significantly differed with their general characteristics(p < .01). 5. As to connections between dental health and the number of existing permanent tooth, 21 permanent teeth or more were owned by those who were in good dental health, who appreciated different tastes and whose chewing ability was good. So there was a statistically significant relationship between the two.

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Amino Acid Biosynthesis and Gene Regulation in Seed (종자내 아미노산 합성 조절 유전자에 관한 연구)

  • ;;;;;Fumio Takaiwa
    • Proceedings of the Botanical Society of Korea Conference
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    • 1996.07a
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    • pp.61-74
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    • 1996
  • Human and monogastric animals can not synthesize 10 out of the 20 amino asids and therefor need to obtain these from their diet. The plant seed is a major source of dietary protein. It is particular important in their study to increase nutritional quality of the seed storage proteins. The low contents of lysine, asparagine and threonenein various cereal seeds and of cystein and methionine. In legume seeds is due to the low proportions of these amino acids in the major storage proteins, we have tried to apply the three strategies; (1) mutagenesis and selection of specific amino acid analogue resistance, (2) cloning and expression study of lysine biosynthesis related gene, (3) transfomation of lysine rich soybean glycinin gene. The 5-methyltryptophan (5MT) resistant cell lines, SAR1, SAR2 and SAR3 were selected from anther derived callus of rice (Oryza sativa L. "Sasanishiki"). Among these selected cell lines, two (SAR1 and SAR3) were able to grow stably at 200 mg/L of 5MT. Analysis of the freed amino acids in callus shows that 5MT resistant cells (SAR3) accumulated free tryptophan at least up to 50 times higher than those that of the higher than of SAS. These results indicated that the 5MT resistant cell lines are useful in studies of amino acid biosynthesis. Tr75, a rice (Oryza sativa L., var. Sasanishiki) mutant resistant to 5MT was segregated from the progenies of its initial mutant line, TR1. The 5MT resistant of TR75 was inherited in the M8 generations as a single dominant nuclear gene. The content of free amino acids in the TR75 homozygous seeds increased approximately 1.5 to 2.0 fold compared to wild-type seeds. Especially, the contents of tryptophan, phenylalanine and aspartic acid were 5.0, 5.3 and 2.7 times higher than those of wild-type seeds, respectively. The content of lysine is significantly low in rice. The lysine is synthesized by a complex pathway that is predominantly regulated by feedback inhibition of several enzymes including asparginase, aspatate kinase, dihydrodipicolinat synthase, etc. For understanding the regulation mechanism of lysine synthesis in rice, we try to clone the lysine biosynthetic metabolism related gene, DHPS and asparaginase, from rice. We have isolated a rice DHPS genomic clone which contains an ORF of 1044 nucleotides (347 amino acids, Mr. 38, 381 daltons), an intron of 587 nucleotides and 5'and 3'-flanking regions by screening of rice genomic DNA library. Deduced amino acid sequence of mature peptide domain of GDHPS clone is highly conserved in monocot and dicot plants whereas that of transit peptide domain is extremely different depending on plant specie. Southern blot analysis indicated that GDHPS is located two copy gene in rice genome. The transcripts of a rice GDHPS were expressed in leaves and roots but not detected in callus tissues. The transcription level of GDHPS is much higher in leaves indicating enormous chloroplast development than roots. Genomic DNA clones for asparaginase genes were screened from the rice genomic library by using plaque hybridization technique. Twelve different genomic clones were isolated from first and second screening, and 8 of 12 clones were analyzed by restriction patterns and identified by Southern Blotting, Restriction enzyme digestion patterns and Southern blot analysis of 8 clones show the different pattern for asparaginase gene. Genomic Southern blot analysis from rice were done. It is estimated that rice has at least 2-3 copy of asparaginase gene. One of 8 positive clones was subcloned into the pBluescript SK(+) vector, and was constructed the physical map. For transformation of lysine rich storage protein into tobacco, soybean glycinin genes are transformed into tobacco. To examine whether glycinin could be stably accumulated in endosperm tissue, the glycinin cDNA was transcriptionally fused to an endosperm-specific promotor of the rice storage protein glutelin gene and then introduced into tobacco genomic via Agrobacterium-mediated transformation. Consequently the glycinin gene was expressed in a seed-and developmentally-specific manner in transgenic tobacco seeds. Glycinin were targeted to vacuole-derived protein bodies in the endosperm tissue and highly accumulated in the matrix region of many transgenic plant (1-4% of total seed proteins). Synthesized glycinin was processed into mature form, and assembled into a hexamer in a similar manner as the glycinin in soybean seed. Modified glycinin, in which 4 contiguous methionine residues were inserted at the variable regions corresponding to the C - teminal regions of the acidic and basic polypeptides, were also found to be accumulated similarly as in the normal glycinin. There was no apparent difference in the expression level, processing and targeting to protein bodies, or accumulation level between normal and modified glycinin. glycinin.

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Analysis of Molecular Epidemiological Properties of Staphylococcus aureus Isolates from Domestic Animals and Human Patients by PCR (Polymerase Chain Reaction을 활용한 국내 동물과 사람환자에서 분리한 Staphylococcus aureus 분리주의 분자역학적 특성분석)

  • Woo Yong-Ku;Kim Shin
    • Korean Journal of Microbiology
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    • v.41 no.1
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    • pp.24-37
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    • 2005
  • This study was conducted to analyze the molecular epidemiological properties and to select the most efficient and reliable PCR method on 116 of Staphylococcus aureus (S. aureus) isolates from Korean cattle, black goat, pig, dog, chicken, mouse and also human clinical cases from hospital. The distribution patterns of SSG [species specific genes; coagulase (coa), protein A (spa), nuclease (nuc) and aroA (RsaI) gene] were analyzed by PCR method. Among the SSGs, the nuc-gene was found in all strains $(100\%)$ tested and followed by coa-gene $(87.9\%)$, spa-gene $(91.4\%)$ and aroA-gene $(26.7\%)$, in order. The genetic subtyping by RFLP method was performed on the coa [AluI] and aroA-gene [RsaI] PCR products. The mecA-gene PCR and PCR-RFLP techniques were chosen to detect and verify of MRSA strains. Only the human strains $(12.1\%)$ were detected the positive mecA-gene products (533 bp), which were divided into two specific bands [201 & 332 bp] by HhaI enzyme digestion. On coa-gene and spa-gene typing, coa-gene was typed with ten kinds of genotype and coa-3 type were determined as the most predominant genotype, while spa-gene was divided into eleven kinds of genotype and also spa-7 type were selected the most prevalent genotype based on their genetic variations. On the aroA and coa-gene subtyping by PCR-RFLP, aroA-gene products were discriminated with only seven types of genotype, while coa-gene products were further divided into an eleven genotype, respectively. In comparison of SID values of five PCR based typing methods, the coa-PCR-RFLP (SID0.894) was evaluated the most efficient and reliable tools and followed by coa-PCR (SID0.883) and aroA-PCR-RFLP (SID0.462), in order. In conclusion, we could determined that the coa-PCR-RFLP method was the most suitable genetic analysis tool for S. aureus and MRSA strains from domestic animals and humans.