• 제목/요약/키워드: differential detection

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Detection and Identification of Mycobacterium Tuberculosis in Patients with Tuberculous Cervical Lymphadenitis by PCR-RFLP (경부 결핵성 임파선염 환자에서 PCR-RELP를 이용한 결핵균의 검출 및 확인)

  • Lee Sang-Sook;Cho Young-Rok;Chun Ji-Min;Choi Yong-Seok;Sohn Eun-Ju;Park Nam-Cho;Park June-Sik
    • Korean Journal of Head & Neck Oncology
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    • v.12 no.2
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    • pp.169-176
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    • 1996
  • Tuberculous cervical lymphadenitis is still an important cause of neck mass in Korea. Tuberculosis is an important differential diagnosis in patients of cervical lymphadenopathy. Rapid and sensitive test for the diagnosis of tuberculosis is essential for the approapiate treatment. Up to now, conventional diagnostic methods for M. tuberculosis were acid-fast bacilli(AFB) stain and culture of M. tuberculosis. The direct microscopic examination of AFB by Ziehl-Neelsen stain is rapid, but often negative. The culture for M. tuberculosis is time-consuming, taking 4 to 8 weeks. Recently various methods to detect Mycobacterial DNA, including PCR and restriction fragment length polymorphism(RFLP) analysis have been reported. Here we represent a simple method for the confirmation of M. tuberculosis and exclusion of the other Mycobacterial species by RFLP analysis and silver staining of polyacrylamide gel electrophoresis after nested PCR for a repetitive DNA sequence(IS986) specific for M. tuberculosis from fresh or paraffin-embedded biopsy specimens. This result leads us to conclude that this method is simple, rapid and possibly applicable to confirm M. tuberculosis and rule out the other Mycobacteria species from the clinical specimens in the clinical laboratories.

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Diagnostic Value of Interleukin 21 and Carcinoembryonic Antigen Levels in Malignant Pleural Effusions

  • Bunjhoo, Hansvin;Wang, Zheng-Yun;Chen, Hui-Long;Cheng, Sheng;Xiong, Wei-Ning;Xu, Yong-Jian;Cao, Yong
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.7
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    • pp.3495-3499
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    • 2012
  • The aim of this study was to evaluate the diagnostic value of interleukin 21(IL-21) and carcinoembryonic antigen (CEA) in tuberculous pleural effusions (TPEs) and malignant pleural effusions (MPEs). Pleural effusion samples from 103 patients were classified on the basis of diagnosis as TPE (n=51) and MPE (n=52). The concentration of IL-21 was determined by ELISA. Lactate dehydrogenase (LDH), adenosine dehydrogenase (ADA) and CEA levels were also determined in all patients. A significant difference was observed in the levels of ADA and CEA (P<0.01), but not in the levels of LDH (P>0.05) between TPE and MPE. The concentration of IL-21 in MPE was significantly higher compared to TPE (P<0.01). With a threshold value of 4.32 pg/ml, IL-21 had a sensitivity of 76.9% (40/52) and a specificity of 80.4% (41/51). Combined detection of IL-21 and CEA had a sensitivity of 69.2% (36/52) and a specificity of 92.2% (47/51). These two markers can contribute to the differential diagnosis of MPEs.

Determination of Hg (II) Ion at a Chemically Modified Carbon Paste Electrode Containing L-Sparteine (L-Sparteine 수식전극을 사용한 Hg (II) 이온의 정량)

  • Euh Duck Jeong;Mi-Sook Won;Yoon-Bo Shim
    • Journal of the Korean Chemical Society
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    • v.35 no.5
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    • pp.545-552
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    • 1991
  • A mercury ion-sensitive carbon-paste electrode (CPE) was constructed with l-sparteine. Mercury (II) ion was chemically deposited by the complexation with l-sparteine onto the CPE. The surface of CPEs was characterized by cyclic voltammetry and anodic stripping voltammetry in an acetate buffer solution, separately. Exposure of CPEs to acid solution could regenerate surface and reuse it for deposition. In 5 deposition/measurement/regeneration cycle, the response was reproducible and in licnear up to $2.0\;{\times}\;10^{-6}$ M with linear sweep voltammetry. In case of using the differential pulse technique, we have obtained the linear response up to $7.0 {\times}10^{-7}$ M with relative standard deviation of ${\pm}5.1$%. The detection limit was $5.0{\times}10^{-7}$ M for 20 minutes of the deposition. We have investigated the interference effect of various metal ions, which are expected to form the complex with ligand. Silver (I) ion of these has interfered with the analysis of Hg (II) ions. However, pretreatment of the silver (I) ion with potassium chloride led to no interference on the analysis of mercury ions in aqueous solution.

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Development of Biotelemetry Method by Combining the SSBL Method and the Pinger Synchronizing Method (2) - Evaluation for Precision of System - (SSBL 방식과 핑거동기 방식을 조합한 바이오텔레메터리 방식의 개발 (2) -시스템의 정도 평가 -)

  • 박주삼;고탁창언
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.39 no.4
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    • pp.318-325
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    • 2003
  • The new biotelemetry method and system that the installation and the treatment of equipment is convenient and the instantaneously detailed position of the fish attached the pinger is able to track comparatively easily had been developed, an availabilities of it were verified in water tank by using hydrophone and pinger. First of all, the receiving system for biotelemetry was calibrated so as to measure tracking of high precision or wide detection range. In the next place, the precision at narrow and wide beam array of receiving system by using hydrophone was investigated and the actual position was compared with measured hydrophone position. The mean standard deviations of the position by narrow beam array of receiving system were 6.4em in phase beam of fore-aft pair and 6.3em in starboard-port pair, and the wide beam array were 24em and 23em respectively. The precision of distance, position, and velocity at narrow beam array of receiving system by using pinger were investigated and the actual values were compared with measured values. The distance from receiving system to pinger was measured by the pinger synchronizing method, angle of direction of pinger was detected by the super short base line (SSBL) method. The three dimensional position of pinger to the receiving system was measured by combining of two kinds of methods (SPB method), the velocity of pinger was obtained with a differential of the three dimensional positions. The mean standard deviations of the distance by pinger synchronizing method in narrow beam array of receiving system was 1. 8 em, that of the position by SPB method was 7.7cm.

SUPPLEMENTAL PERMANENT MAXILLARY LATERAL INCISORS(DOUBLE LATERAL INCISORS) : CASE REPORT (상악 측절치에 발생한 보충치형의 과잉치 : 증례보고)

  • Jung, Mi-Sang;Hyun, Hong-Keun;Kim, Young-Jae;Jang, Ki-Taeg;Lee, Sang-Hoon;Kim, Chong-Chul;Hahn, Se-Hyun;Kim, Jung-Wook
    • Journal of the korean academy of Pediatric Dentistry
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    • v.38 no.1
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    • pp.75-81
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    • 2011
  • Supernumerary teeth are in excess of the normal number of teeth in either the primary or permanent dentitions. They are classified into supplemental teeth resembling those of the normal series and rudimentary teeth with abnormal shapes, according to their form. Most of the supernumerary teeth are rudimentary form, and supplemental teeth are much less common. Sulppemental teeth are most common in the permanent maxillary lateral incisor area and clinicians should be careful with differential diagnosis from normal teeth. Unerupted supernumerary teeth may produce several complications such as delayed eruption, displacement of permanent teeth, diastema, root resorption and cyst formation. Early detection and proper treatment plan according to the tooth alignment and root formation stage are important. Here we report 3 cases of unilateral or bilateral normal incisor shaped supernumerary lateral incisors treated by eruption observation, surgical extraction and orthodontic treatment with resin build-up.

Voltammetric Determination of Cu(II) Ion at a Chemically Modified Carbon-Paste Electrode Containing 1-(2-pyridylazo)-2-naphthol (1-(2-Pyridylazo)-2-naphthol 수식전극을 사용한 Cu(II) 이온의 전압전류법적 정량)

  • Jun-Ung Bae;Hee Sook Jun;Hye-Young Jang
    • Journal of the Korean Chemical Society
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    • v.37 no.8
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    • pp.723-729
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    • 1993
  • Cu(II) ion-responsive chemically modifed electrodes (CMEs) were constructed by incorporating 1-(2-pyridylazo)-2-naphthol (PAN) into a conventional carbon-paste mixture of graphite powder and Nujol oil. Cu(II) ion was chemically deposited on the surface of the PAN-chemically modified electrode in the absence of an applied potential by immersion of the electrode in a buffer solution (pH 3.2) containing Cu(II) ion, and then reduced at a constant potential in 0.1 M KNO$_3$. And a well-defined voltammetric peak could be obtained by scanning the potential to the positive direction. The electrode surface could be regenerated with exposure to acid solution and reused for the determination of Cu(II) ion. In 5 deposition / measurement / regeneration cycles, the response could be reproduced with 6.1${\%}$ relative standard deviation. In case of using the differential pulse voltammetry, the calibration curve for Cu(II) was linear over the range of 2.0 ${times}$ 10$^{-7}$ ∼ 1.0 ${times}$ 10$^{-6}$ M. And the detection limit was 6.0 ${times}$ 10$^{-8}$ M. Studies of the effect of diverse ions showed that Co, Ni, Zn, Pb, Mg and Ag ions added 10 times more than Cu(II) ion did not influence on the determination of Cu(II) ion, except EDTA and oxalate ions.

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Design of Optimized RBFNNs based on Night Vision Face Recognition Simulator Using the 2D2 PCA Algorithm ((2D)2 PCA알고리즘을 이용한 최적 RBFNNs 기반 나이트비전 얼굴인식 시뮬레이터 설계)

  • Jang, Byoung-Hee;Kim, Hyun-Ki;Oh, Sung-Kwun
    • Journal of the Korean Institute of Intelligent Systems
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    • v.24 no.1
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    • pp.1-6
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    • 2014
  • In this study, we propose optimized RBFNNs based on night vision face recognition simulator with the aid of $(2D)^2$ PCA algorithm. It is difficult to obtain the night image for performing face recognition due to low brightness in case of image acquired through CCD camera at night. For this reason, a night vision camera is used to get images at night. Ada-Boost algorithm is also used for the detection of face images on both face and non-face image area. And the minimization of distortion phenomenon of the images is carried out by using the histogram equalization. These high-dimensional images are reduced to low-dimensional images by using $(2D)^2$ PCA algorithm. Face recognition is performed through polynomial-based RBFNNs classifier, and the essential design parameters of the classifiers are optimized by means of Differential Evolution(DE). The performance evaluation of the optimized RBFNNs based on $(2D)^2$ PCA is carried out with the aid of night vision face recognition system and IC&CI Lab data.

Ultrasonographic Features of Intra-abdominal Abscess (복부내 농양의 초음파 소견)

  • Cho, Kil-Ho;Jung, Kyung-Hee;Hwang, Mi-Soo;Chang, Jae-Chun;Kwun, Koing-Bo;Min, Hyun-Sik
    • Journal of Yeungnam Medical Science
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    • v.2 no.1
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    • pp.87-93
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    • 1985
  • Intraabdommal abscess usually causes distress with fever, leukocytosis, pain and toxicity. Diagnosis of intraabdominal abscess is occasionally difficult and It has high morbidity. However radiologic method, such as ultrasonography, CT scan, or RI scan are helpful to early detection of intraabdominal abscess. Among these methods, ultrasonography is a non-invasive technique and performed without discomfort to patient. And also differential diagnosis between cystic and solid lesion is very easy and sequential ultrasonography in same patient is valuable for the evaluation of treatment effect. We analyzed the ultrasonic features of 48 cases with intra-abdominal abscesses and the results are as follows; 1. In total 48 cases, the intra-abdominal abscesses were 30 cases, the retroperitoneal abscesses, 5 cases, and the visceral abscesses, 13 cases. 2. The causes of the intra-abdominal abscesses were perforating appendicitis (25 cases), postoperative complications (5 cases), pyogenic and amebic hepatic abscesses (13 cases), and the others (5 cases). 3. Round or oval shaped lesions were 26 cases (54%), irregular shape, 18 cases (38%), and multiple abscess formation in 4 cases (8 %). 4. The size of the lesions were between 5 and 10cm in diameter in 54% of total 48 cases, and the most frequent feature of the echo-pattern of the lesions was cystic with or without internal echogenicity (69%).

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Clinical Manifestations of Ureteral Duplication in Children (소아 중복 요관의 임상적 고찰)

  • Han Jung-Woo;Hwang Dae-Hwan;Park Jee-Min;Han Sang-Won;Lee Jae-Seung
    • Childhood Kidney Diseases
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    • v.7 no.2
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    • pp.189-196
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    • 2003
  • Purpose : Ureteral duplication is a common urologic anomaly and early diagnosis may prevent irreversible renal damage. We thus aimed to evaluate the benefits of prenatal sonography in early detection. Methods : We retrospectively studied 55 children with duplicated systems who were admitted to Severance Hospital, Yonsei University, Seoul, Korea from January 1998 to July 2003. Results : The mean age at diagnosis was 2.3 years old. 89% of the children had complete duplicated ureter. 47% of the children visited the hospital with an initial complaint of abnormal prenatal sonographic findings. Among these patients, 70.8% had DMSA defects. The mean differential renal function(DRF) in the DMSA scan was 48.2% in the group diagnosed prenatally. In the postnatally-diagnosed group, DMSA defects were found in 67.7% patients and the mean DRF was 45.5%. Conclusion : In comparison with the past studies, the mean age at diagnosis is becoming younger, and the proportion of abnormal prenatal sonography as an initial complaint larger. Other clinical manifestations were similar. The difference of the renal damage between the prenatally diagnosed group and the postnatally diagnosed group was not statistically significant. A multi-center study may help to prove the importance of prenatal sonography in early diagnosis and treatment of ureteral duplication. (J Korean Soc Pediatr Nephrol 2003;7:189-196)

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A Fast Sensing Method using Concurrent Driving and Sequential Sensing for Large Capacitance Touch Screens (동시구동 및 순차센싱을 이용한 대형 정전용량 터치스크린용 고속 센싱 기법)

  • Mohamed, Mohamed G.A.;Kim, HyungWon;Cho, Tae-Won
    • Journal of the Institute of Electronics and Information Engineers
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    • v.52 no.4
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    • pp.62-70
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    • 2015
  • Recently the demand for projected capacitance touch screens is sharply growing especially for large screens for medical devices, PC monitors and TVs. Large touch screens in general need a controller of higher complexity. They usually have a larger number of driving and sensing lines, and hence it takes longer to scan one frame for touch detection leading to a low frame scan rate. In this paper, a novel touch screen control technique is presented, which scans each frame in two steps of simultaneous multi-channel driving. The first step is to drive all driving lines simultaneously and determine which sensing lines have any touch. The second step is to sequentially rescan only the touched sensing lines, and determine exact positions of the touches. This technique can substantially increase the frame scan rate. This technique has been implemented using an FPGA and an AFE board, and tested using a commercial 23-inch touch screen panel. Experimental results show that the proposed technique improves the frame scan rate by 8.4 times for the 23-inch touch screen panel over conventional methods.