• Journal of Nutrition and Health
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• 제22권6호
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• pp.485-496
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• 1989

This study was performed to investigate nutritional effect of various dietary fibers on lead absorption and metabolism of protein and lipid in growing rats. Forty eight male rats of Sprague-Dawley strain weighing 75.7$\pm$0.7g were blocked into six groups according to body weight and fed six kinds of diet different with fiber source(non-fiber, cellulose, pectin) and lead level(0%, 1% ) for 4 weeks. Results are summerized as follows: 1) Food intake, weight gain, FER and PER were remarkably decreased in lead(Pb) added groups, and FER and PER in Pb-added pectin group were significantly lower than those in Pb-added non-fiber group. 2) Weight of liver, kidney and epididymal fat pad, bone weight and length, hematocrit, and hemoglobin content were decreased in Pb-added groups. 3) Total protein content in serum was tended to be decreased in Pb-added groups, but total lipid and cholesterol contents in serum were not different with dietary Pb level and fiber source. 4) Nitrogen, lipid and cholesterol content in liver were tended to be deceased in Pb-added groups, and especially those of the Pb-added pectin group were the lowest among groups. 5) Daily urinary and fecal excretions of nitrogen, lipid and cholesterol were decreased in Pb-added groups. Especially fecal excretions of nitrogen, lipid and cholesterol in Pb-free groups were significantly increased by dietary cellulose and pectin. 6) Pb content in blood was significantly increased in Pb-added pectin group. There was no significant decrease in Pb contents of liver, kidney and tibia, and increase in excretion of Pb by feeding dietary fibers. In conculsion, dietary fibers had no effect on the absorption of Pb, and dietary pectin seemed to increase Pb poisoning by decreasing bioavailibility of protein, lipid and other nutrients in the diet.

• Asian-Australasian Journal of Animal Sciences
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• 제5권2호
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• pp.369-374
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• 1992

In order to determine the effect of dietary cellulose levels on chick performance, 1-week-old White Leghorn male chicks were fed semi-purified diets containing either 5%, 10%, 15% or 20% cellulose for 3 weeks. All diets were iso-nitrogenous and iso-caloric. Feed intake and body weight were increased as the cellulose level was increased. Feed conversion was lower in the 5% cellulose diet than in the other diets. Compared to the case of 10% to 20% cellulose diets, relative length (per 100 g body weight) of esophagus, duodenum, small intestine and cecum was longer, and relative weight of esophagus, duodenum and crop was also heavier in the chicks fed the 5% cellulose diet. The other internal organs were not affected by the cellulose level. The retention rates of dry matter and nitrogen were lower in the 5% cellulose group than in the other groups. Concentration of carcass phospholipids was higher in the 5% cellulose group, and that of carcass cholesterol was increased as the cellulose level increased. In conclusion, the dietary cellulose level should be more than 5% to give an adequate chick performance, and no ill effect was found even at 20% level.

• Asian-Australasian Journal of Animal Sciences
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• 제16권12호
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• pp.1777-1781
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• 2003

The lysine requirements of juvenile yellowtail flounder (Pleuronectes ferrugineus) having 19.5 g initial body weight were estimated by feeding six practical-type diets containing graded levels of lysine (1.21 to 2.69% of dry diet). Dietary amino acid profile simulated that of whole body of yellowtail flounder. Most of amino acids in the diets were provided by corn gluten meal, herring meal and gelatin. Protein efficiency ratio (PER) improved significantly until lysine level increased up to 2.1% (4.3% of protein). Same trend was observed in feed:gain ratio (FGR) which maintained constant in fish groups fed diets containing lysine above 2.1%. The highest nitrogen gain (0.34 g/fish) in whole body was found in fish fed 2.1% lysine, though the value was not different from those of fish fed above the level of lysine. Fish fed 2.1% lysine also showed the best nitrogen retention efficiency of 24.6%. The broken-line analysis of protein efficiency ratio and body nitrogen gain against dietary lysine level yielded an estimated lysine requirement of 2.2% (4.5% of protein) and 2.3% (4.7% of protein), respectively.

• 동아시아식생활학회지
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• 제19권1호
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• pp.45-51
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• 2009

In this study, food protein hydrolysates were prepared from six types of food protein: purified meat protein, whole egg protein, casein, isolated soy protein, concentrated rice protein, and gluten. Food proteins were hydrolyzed with pepsin and ethanol (80%)-soluble fractions of pepsin hydrolysates were employed for analysis. The products were colorless and odorless powders with low fat content and good solubility. The MW (molecular weight) of the protein hydrolysates was confirmed to be $200{\sim}1,800$ via gel filtration. Free amino acid contents accounted for less than 5% of the samples. The results of our amino acid analysis revealed that all food protein hydrolysates preserved their original amino acid compositions and nutritional values of their source proteins with highly pure oligopeptide mixtures. These results show that the food protein hydrolysates prepared in these investigations should prove excellent dietary nitrogen sources for a variety of applications.

• 한국가정과교육학회지
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• 제24권2호
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• pp.51-62
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• 2012

본 연구는 1962년 한국인 영양권장량의 제정부터 2010년 한국인 영양섭취기준 개정에 이르기까지 성인과 임신 수유부의 단백질의 섭취권장량 산출방법과 섭취권장량의 변화 추이를 살펴보았다. 1. 성인의 단백질 섭취권장량은 1989년까지 요인가산법을, 그 이후에는 질소균형연구를 활용하여 설정되었다. 요인가산법을 적용하였던 시기는 표준단백질 최소 필요량 또는 불가피 질소손실량을 기본 요인으로 하고 식이단백질의 이용율을 적용하였으며, 질소균형연구를 적용하였던 시기는 질소평형 유지에 필요한 식이단백질의 최소 필요량을 기본 요인으로 하였다. 그리고 개인 차, 스트레스 등의 조정 요인을 반영하여 단백질 섭취권장량을 계산하였다. 단백질 섭취권장량은 남성 50~80g/일, 여성 45~70g/일이었으며, 남성의 섭취권장량이 여성보다 크고, 연령대가 높을수록 섭취권장량은 감소하였다. 2. 임신부의 단백질 부가섭취권장량은 태아의 발육에 기인하는 단백질 축적량을 기본 요인으로 하고 식이단백질 이용율 등의 조정요인을 적용하여 산출하였고, 10~30g/일이었으며, 2010년에는 임신 기간을 3분하여 각각 0, 15, 30g/일을 제시하였다. 3. 수유부의 단백질 부가섭취권장량은 모유로 분비되는 단백질의 양을 기본 요인으로 하고 식이단백질 이용율 등의 조정요인을 적용하여 산출하였고, 20~30g/일이었으며, 시기별 증감 경향은 나타나지 않았다. 앞으로 체중 대신 제지방체중을 적용하는 단백질 섭취권장량의 산출 공식이 마련될 필요성이 있으며, 에너지와 마찬가지로 섭취하는 식이단백질의 조성에 근거한 개인별 섭취권장량의 계산방법 고안을 제언한다. 그리고 수유부의 경우 수유 기간을 구분하여 기간별 단백질 부가섭취권장량의 제정을 제언한다.

• Asian-Australasian Journal of Animal Sciences
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• 제12권8호
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• pp.1298-1309
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• 1999

The nutritional value of protein varies between feedstuffs. It is possible to feed animals using crystalline amino acids as a sole nitrogen source, but in practice only some limiting amino acids are added to the diet. In order to use feedstuffs efficiently, it is important to determine exact amino acid requirements. Reported values differ widely because the requirements are affected by various factors. In this report, therefore, the factors affecting amino acid requirements are reviewed as follows: 1) availability of dietary amino acids, conversion factors of nitrogen to protein, interaction of amino acids, and strain, sex and age of animals; 2) amino acid requirements for maximum performance and maintenance, usefulness of non-essential amino acids; 3) plasma amino acid concentration as a parameter to determine amino acid requirements; and 4) nitrogen excretion to reduce environmental pollution. These factors should be considered, it is to improve the dietary efficiency, which is to reduce excess nitrogen excretion for environmental pollution.

• Asian-Australasian Journal of Animal Sciences
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• 제2권3호
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• pp.544-545
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• 1989

• 동아시아식생활학회지
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• 제23권1호
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• pp.92-97
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• 2013

This study was conducted to understand the quality characteristics of salt fermented anchovies with heat treatment by measuring their chemical compositions. The heat-treated and non-heat treated salt fermented anchovies contained, respectively, 63.21 and 66.51% of moisture, 2.24 and 2.12% of total nitrogen (TN), and 1,537 and 1,520 mg/100 g of amino nitrogen (AN). In addition, heat-treated and non-heat-treated salt fermented anchovies contained 127 and 134 mg/100 g of volatile basic nitrogen (VBN), respectively. Moreover, measured the microbial level of heat-treated and non-heat-treated salt fermented anchovies was $2.58{\times}10^4$ and $3.61{\times}10^2$ CFU/mL, respectively. Also, the heat-treated and non-heat-treated salt fermented anchovies 3.65 and 0.30 units of protease activities, respectively. The total free amino acid contents in heat-treated and non-heat-treated salt fermented anchovies was 4,964 and 6,638 mg/100 g, respectively. The major free amino acid were glutamic acid, leucine, lysine, alanine, valine, isoleucine. Our results provide the characteristics of salt fermented anchovies and encourage their application for the food industry and cooking.

• Asian-Australasian Journal of Animal Sciences
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• 제13권12호
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• pp.1667-1673
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• 2000

The climate and marketing system of raw milk in Taiwan create problems in balance feeding of protein and energy in lactating cows in Taiwan. Level of urea nitrogen both in bulk milk and serum reflects ruminal protein degradation and post-ruminal protein provision, whereas milk protein concentration responds to dietary energy intake and bacterial protein production in the rumen. Establishment of a range of reference standards in milk protein and urea nitrogen levels can be applied as a noninvasive economical feeding guide to monitor the balance of protein and energy intake. Standard reference levels of 3.0% milk protein and 11-17 mg/dL milk urea nitrogen (MUN) were established. Level of milk protein below 3.0% is regarded as indicating inadequate dietary energy whereas MUN below or above the range is regarded as a deficiency or surplus in dietary protein. Results from analysis of bulk a milk samples collected from 174 dairy herds over Taiwan showed that only one quarter (25.29%) of the herds received a balanced intake of protein and energy, 33.33% adequate protein with energy inadequate, 22.99% herds in protein surplus with energy inadequate, 10.35% herds in protein surplus with energy adequate, 4.6% protein deficiency with energy adequate, and 3.45% herds with both protein and energy inadequate. Energy inadequate herds accounted for 60% of the total dairy herds in Taiwan with 56% adequate, 38% surplus and 6% inadequate in protein. In comparing milk sampled from bulk milk on different seasons from Lee-Kang area in the southern Taiwan, the concentrations of milk fat and milk protein were significantly higher in the cool season (February) than in the warm season (August) (p<0.05), whereas the urea nitrogen in the milk was significantly lower in the cool season than in the warm season (p<0.05). This indicated that lactating cows had excess protein and/or inadequate energy intake in the warm season in this area. It appears that the major problem feeding in lactating cows is energy intake shortage, especially during the warm season in Taiwan.

• Asian-Australasian Journal of Animal Sciences
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• 제6권2호
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• pp.235-242
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• 1993

In order to investigate the effects of dietary cellulose and protein levels on chick performance, four semi-purified diets were formulated so as to contain cellulose at levels of 5% (LC) and 20% (HC) in combination with 10% (LP) and 20% (HP) protein, and fed ad libitum to 1-week-old White Leghorn male chicks for 3 weeks. There were no significant differences in feed intake, body weight gain and feed efficiency between the LC-HP and HC-HP groups. All parameters were lower in the LP groups; the HC-LP group consumed very small amount of feed and lost body weight during the experiment. The retention rates of DM, ash, nitrogen and energy were higher in the HP than the LP groups. The triglyceride concentration of carcass was lower in the HC-LP group and that of liver was higher in the LC-LP group. The carcass total cholesterol level was higher in the HC-HP group. The relative weight of most digestive organs was higher in the HP group irrespective of the cellulose level. In conclusion, the chick performance was primarily influenced by dietary protein level, and when the chicks were fed inadequate levels of protein, the low cellulose level gave a better performance than the high cellulose level.