• Journal of Nutrition and Health
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• v.34 no.2
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• pp.123-131
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• 2001

The purpose of this study was to investigate the effects of diet composition on serum leptin and lipids profiles in rats. At the baseline, seven 4-week-old Sprague-Dawley rats, male, were sacrificed and the remaining rats were divided into five groups and each group was fed one of the following five dietary regiments for 2 weeks and 6 weeks;the control diet AIN76(CAL, n=2l), high-carbonhydrate(rice)(HCR, n=2l), high-carbohydrate(flour)(HCF, n=2l), high-fat(corn oil)(HFO, n=2l), high-fat(beef tallow)(HFB, n=2l). Serum leptin was determined by a double antibody ELISA assay at the baseline(n=7), 6 week(n=35) and 10 week of age(n=70). At 6 weeks of age, the increase in the Food Efficiency Ratio(FER) was related to adipocyte hyperplasia in rats on HFB diets. The serum triglyceride, total cholesterol, and LDL-cholesterol increased significantly in HFB group, and decreased in HFO group compared to control group. The HFC group showed significant increase in serum triglyceride level compared with control group. After 2 weeks and 6 weeks, noticeably high increases in epididymal adipose tissue fat cell mass and numbers were observed with the HFB fed group. Serum leptin levels increased as body weight increased over the period of time(4weeks; 1.50$\pm$0.13ng/ml versus 10weeks; 2.08$\pm$-.13ng/ml). And this result shows that there are 193% higher in rats fed high fat-beef tallow diet than the control diet. Serum leptin levels of the HFB group(4.01$\pm$0.39mg/ml) were significantly higher than that of the HFO(2.06$\pm$0.5613ng/ml), CAL(2.08$\pm$0.1313ng/ml), HCR(2.41$\pm$0.2113ng/ml) and HCF(2.80$\pm$0.4713ng/ml) at p<0.05. The serum leptin concentration was positively correlated with the amount of epididymal fat pads(r=0.47 p<0.01), serum triglyceride(r=0.49, p<0.001), tatal cholesterol(r=0.48, p<0.001), LDL-cholesterol(r=0.58, p<0.001), atherogenic index(r=0.67, p<0.001), and inversely correlated with HDL-cholesterol(r=-0.65, p<0.001). In conclusion, the changes in composition of dietary fat and carbohydrate intake could affect changes in concentration of serum lipids and leptin. Especially, the high-fat diet with animal fat source could increase circulating leptin level. (Korean J Nutrition 34(2) : 123-131, 2001)

• The Korean Journal of Food And Nutrition
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• v.30 no.3
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• pp.531-538
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• 2017

This study was performed to investigate the effects of garlic on uncoupling protein 2 (UCP2) transcriptional regulation of UCP2-luciferase transgenic mice fed on a high fat diet to induce obesity. To examine the transcriptional regulation of UCP2, we generated transgenic mice with a UCP2 promoter (-1,830/+30 bp) containing luciferase as a reporter gene. UCP2-luciferase transgenic mice were fed a 45% high-fat diet for 8 weeks to induce obesity. Subsequently, mice were maintained on either a high-fat control diet (TG-CON), or high-fat diets supplemented with 2% (TG-GL2) or 5% (TG-GL5) garlic for a further 8 weeks. Dietary garlic reduced body weight and energy efficiency ratio in the TG-GL5 group, compared to the TG-CON group. Furthermore, garlic supplementation significantly decreased white adipose tissue fat mass and plasma levels of triglycerides, total cholesterol, and leptin in the TG-GL2 and TG-GL5 groups, compared to the TG-CON group. Specifically, UCP2 promoter activity in metabolic tissues such as liver, white adipose tissue, brown adipose tissue, and skeletal muscle was increased by garlic supplementation. These results suggest that dietary garlic was partially associated with an increase of UCP2 transcriptional activity in metabolic tissues for decreasing obesity.

• Journal of Nutrition and Health
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• v.5 no.4
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• pp.169-176
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• 1972

The present study was undertaken to determine the metabolic effect of various levels of fat in the diet. Forty males and the same number of females weighing $35{\pm}29$ were divided into three experimental groups and one control group, 10 rats each in both sexes. The dietary lipid contents were included in three levels, 2% as low, 30% as high and fat free diet in order to reflect the lipid consumption of present Korean diet. 20% sugar casein diet were employed as standard for control animals. This study was carried for 16 weeks. After these period animals were sacrificed to collect the internal organs and blood samples by heart puncture. In the result of this study, high fat diet group is lower than low fat diet group in the body weight gain, food efficiency ratio, protein efficiency ratio, urinary nitrogen and organ nitrogen contents were same trend but there is no significant difference in these respects. It was noteworty that high, and free fat diet group revealed more glucose total protein, albumin and globulin contents in the serum than other compared groups. It can be concluded that fat content whether low or high are free in the diet did not show significant effect on body nitrogen metabolism. But 30% high fat diet increased the total lipid and total cholesterol contents in the liver and the serum. This fact can be interpreted that 2% low fat diet and fat free diet do not need to increase the amount of fat content in the diet as high as 30% fat diet. This result might indicate the one possible reason to decide the recommanded dietary fat levels in Korean diet.

• Journal of the East Asian Society of Dietary Life
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• v.16 no.6
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• pp.663-669
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• 2006

The dietary effects of soy fiber beni-koji(SBCA) containing chitosan-ascorbate on the body weight and serum lipids in obese rats induced by a high fat diet were investigated. The experimental plots(6 rats per each group) were divided into a normal group(NC), a high fat diet group(HF), a 1% SBCA diet group(SBCA1) and a 2% SBCA diet group(SBCA2), and fed for 6 weeks. Compared with the HF group, the mean body weight and the $R\'{o}hrer$ index of the SBCA1 and SBCA2 groups decreased by $3.4{\sim}7.4%$ and $8.1{\sim}13.9%$, respectively. In particular, the body lipids in the SBCA2 group decreased by 42.3%. The serum triglyceride content decreased by approximately $25.54{\sim}27.34%$ in the experimental diet groups. There was no significant difference in the serum total lipids between the HF and SBCA1 groups. On the other hand, the total lipids in SBCA2 group decreased by 19.12% compared with the HF group. In the SBCA2 group, the total serum cholesterol and LDL-cholesterol content decreased by 6.57% and 41.20% compared with the HF group, respectively, while the HDL-cholesterol increased by 10.0%. The risk factor index(RFI) in the SBCA2 group was decreased remarkably by 58.57%.

• Journal of Nutrition and Health
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• v.38 no.6
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• pp.465-474
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• 2005

The purpose of this study was to investigate the dietary habits, nutritional status, and serum copper and zinc concentrations of adolescent athletes. 50 high school adolescent athletes (29 male, 21 female) took part in physical education high school and 47 high school adolescent non-athletes (21 male, 26 female) in general high school. Questionnaires, anthropometric measurements, 24-hour dietary recalls and blood analysis were conducted. In the males, the mean height, weight and body fat percents of athletes and non-athletes were 174.0/172.9 cm, 67.4/68.3 kg, and 8.9/20.0% (p<0.001) respectively. In the females, the mean height, weight and body fat percents of athletes and non-athletes were 163.7/159.3 cm (p<0.01), 58.7/55.7 kg (p<0.05), and 18.6/30.1 % (p<0.001) respectively. In both male and female athletes, intakes of animal fat were significantly higher and intakes of vitamin C were significantly lower than those of non-athletes. There was no significantly difference in zinc and copper intakes between athlete and non-athlete groups. The average serum zinc level of male athletes was significantly lower than that of male non-athlete. The average serum copper levels of male and female athletes were significantly lower than those of male and female non-athletes. Based on these results, exercise may effect on zinc and copper utilization of adolescent. Further studies on zinc and copper nutrition of adolescent athletes were needed to understand more mineral nutrition and exercise.

• Journal of the East Asian Society of Dietary Life
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• v.6 no.1
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• pp.59-68
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• 1996

The present study undertaken to investigate the interrelationship among their regular dietary fatty acid intake, body fat content(BFC), and plasma lipid fractions(PLF) of middle-aged Korean women. Forty-nine women (age 30 to 49) participated in this study in which the aim was to determine their total nutrient intake per day, BFC and PLF, and to analyse the intercorrelations of these. Strong correlation was seen among their body mass index, mid-upper-arm-circumference, and BFC which were positively correlated with plasma total lipids(TL) and triglycerides(TG). Dietary myristic acid and lauric acid were positively correlated with both total cholesterol(TC) and LDL-cholesterol (LDL-C) in plasma. Palmitic acid, stearic acid, oleic acid, and total monounsaturated fatty acid(MUFA) were negatively correlated with HDL-cholesterol(HDL-C). Although dietary total saturated fatty acid (SFA) was not correlated with TC and LDL-C, it was positively correlated with TL and TG in plasma. However, dieary n-3 polyunsaturated fatty acid(PUFA) in regular Korean diet was not correlated with any of lipid fractions in plasma which might be due to the high ratio of n-6/n-3 in their diet. From the above results, it was concluded that plasma lipid fractions were modulated by their dietary nutrients, especially the types of the fatty acids. Dietary SFA and MUFA intake not PUFA are strongly correlated with LDL-C and HDL-C in the Korean diet This may relate to the frequency of coronary heart disease in the age group.

• Journal of Nutrition and Health
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• v.27 no.10
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• pp.967-978
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• 1994

This study was done to investigate the effect of age and dietary linolenic acid content and the linolenic acid/linoleic acid(LNA/LA) ratio on the lipid metabolism and formation of PGI2 and TXA2. The male Sprague-Dawley rats were fed 6 different with 0.2, 0.4, 0.6 of LNA/LA ratio within either 8% LNA(high LNA) or 4% LNA(low LNA) of fatty acid content for different feeding period(1, 4, 12 month). The dietary fat used were sesame oil, perilla oil, soybean oil and beef tallow. The concentration of serum total lipid, total cholesterol and HDL-C were increased with aging. Triglyceride concentration was decreased in 0.2 ratio of LNA/LA. The lipid content of liver showed similar tendency to that of serum. The ratio of PGI2/TXA2 was increased in 1 month rats and antithrombotic effect was reduced significantly with increasing age. The PGI2/TXA2 ratio was tended to be higher in diet of 0.2 and 0.4 LNA/LA ratio at high LNA level and in diet of 0.6 LNA/LA ratio at low LNA level. Especially PGI2/TXA2 ratio was increased linearly with rising LNA/LA ratio at low LNA level. It seemed that the LNA content and LNA/LA ratio had interaction to increase the antithrombotic effect bychanging TXA2 synthesis. And the dietary fatty acid related effect lowering the serum and liver lipid content, excepting triglyceride, was increased when dietary n3/n6 ratio was high(0.6) at both high and low n3 level. Therefore, it could not be recommended to consume large amount of n3 fatty acid or high ratio of n3/n6 to prevent cardiovascular diseases. These results suggested that the dietary fatty acid ratio of n3/n6 could be determined based on the n-3 content of dietary fat to reduce the risk of cardiovascular disease.

• Journal of Nutrition and Health
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• v.35 no.1
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• pp.124-136
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• 2002

As the elderly population has increased in recent years in Korea chronic degenerative diseases prevalent in the aged have garnered attention in relation to food behavior. In general, dietary intakes of elderly Koreans were inadequate in quality as well as in quantity. Especially, intakes of Ca, vitamin A, and riboflavin along with total energy were insufficient. They tended to consume high carbohydrate and low fat energy rates which may be resulted from long standing vegetable-based flood patterns. Relationships between dietary intakes and indices of cardiovascular diseases revealed that blood pressures and serum lipids patterns tended to be poor in individuals having high abdominal fat accumulations which seemed to be related with dietary consumption of high carbohydrate and insufficient intake of many nutrients. These results suggested that a nutritionally balanced diet including animal sources floods is needed in order to prevent an abdominal obesity and cardiovascular diseases. The bone health status of the Korean elderly appeared poor. It was more serious in women recording a higher prevalence rate of osteopenia and osteoporosis than in men. There were strong associations between dietary factors and bone health status. Bone mineral density was found to be higher with higher intakes of protein energy rate, the total amount of feeds, and many nutrients including Ca and p, indicating that a good quality diet is essential in maintaining healthy bone status in later life. mental health condition of the elderly as measured by cognitive function and the degree of depression was also inadequate and had strong association with dietary consumption. The positive results were obtained in individuals having an adequate dict. Therefore, it could be summarized that the physical as well as mental health in elderly Koreans are highly related with their dietary patterns. Thus, the older adults need te consume nutritionally well balanced diet in sufficient amount, which contains various flood items including significant amounts of animal source floods in a daily diet in order to maintain healthy condition.

• Journal of Nutrition and Health
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• v.34 no.8
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• pp.858-864
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• 2001

The study was designed to observe an antioxidant activities of conjugated linoleic acid(CLA) in rat liver by determining the activities of antioxidative enzymes(superoxide dismutase, glutathione peroxidase, catalase) and the levels of tocopherol and thiobarbituric acid reactive substance(TBARS). Male Sprague Dawley rats at weeks-old were divided into groups according to the types of dietary fat(beef tallow and fish oil) and then each group was subdivided into groups depending on CLA supplement. All rats were fed experimental diet containing 12% total fat including 1% CLA by weight for 30 weeks. CLA supplemented to beef tallow diet did not have significant effect on the level of TBARS and tocopherol. The level of TBARS was significantly increased in fish oil diet(highly unsaturated fat diet), but its level was significantly reduced by increasing SOD and GSH-Px activities when CLA was supplemented to fish oil diet so that CLA showed a sparing action of tocopherol in tissue. CLA did not have significant effect on peroxisomal catalase activities, but its activity was significantly increased when TBARS production was high in the fish oil diet. CLA could be incorporated into phospholipid of microsomal membrane, and interfered the conversions of C18 : 0 into C18 : 1 and C18 : 2 into C20 : 4 in liver. In conclusion, CLA had an antioxidant activities depending on the type of fat in diet. Therefore, it could be recommended to use CLA when highly unsaturated fat was used in meal preparation.

• Journal of Nutrition and Health
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• v.31 no.2
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• pp.143-152
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• 1998

This study was conducted to investigate the effects of chitosan, chitosan oligomer and beef tallow of different levels on lipid metabolism in rats, Seventy male rats of the Sprague-Dawley strain were blocked into 10 groups according to body weight , and were raised for 4 weeks. Dietary fat levels were 20% en and 40% en, and chitosan and chitosan oligomer were given at levels of 05, 3%, and 5%(wt/wt) of diet. The results are summarized as follows, chitosan oligomer supplement decreased serum total lipid, as chitosan 양, and exhibited a tendency to decrease serum total cholesterol. Chitosan oligomer supplement tended to increase the HDL cholesterol ; total cholesterol ration as chitosan did. Liver total lipid and triglyceride concentration were lower in high fat groups than in low fat groups. Liver total lipid concentrations was decreased slightly by chitosan and sigfnificantly by chitosna ligomer. Epididymal fat pad total lipid. total cholesterol and triglyceride concentration were slightly decreased by chitosan and chitosan oligomer supplement, especially in high fat groups. fat absorptivity was decreased by low fat level, chitisan and chitosan oligamer supplement. Fecal excretion of total lipid and triglyceride were increased by high fat level, chitosan and chi샌무 oligomer supplement. However, fecal excretion of total cholesterol was increased by high fat level and chitosan supplement. This indicated that chitosan and chitosan oligomer were effective in interfering with lipid and triglyceride absorption, In conclusion, chitosan oligomer at levels of 3% and 5% has more effective lipid, cholesterol and triglyceride lowering activity than chitosan.