• Asian-Australasian Journal of Animal Sciences
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• 제31권5호
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• pp.712-720
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• 2018

Objective: The aim of this experiment was to evaluate the effect of dietary supplementation with the docosahexaenoic acid (DHA)-rich microalgae, Aurantiochytrium limacinum (AURA) on pig performance, carcass traits, and the fatty acid composition of pork Longissimus lumborum (LL) and backfat. Methods: A total of 144 Pig Improvement Company (PIC)${\times}$Goland finishing pigs (72 females and 72 castrated males) of mean weight 117.1 (${\pm}13.1$) kg were blocked by sex and body weight and provided with 0% or 1% AURA in isonutritive and isocaloric diets. A total of 24 pens provided 12 replicates per treatment. Animals were weighed on day 0 and 28 with feed and water intake recorded per pen. After 31 days supplementation (28 days of study and 3 days until the slaughtering date) three animals per pen (n = 72) were slaughtered and the LL and backfat thickness, lean meat content and dressing percentage were recorded for the carcasses. The fatty acid (FA) profile of the LL and backfat was established by direct FA methyl ester synthesis. Results: No differences were observed for any performance parameters or carcass traits. Supplementation with AURA resulted in significant changes to the FA profiles of both the LL and backfat with male and female pigs responding differently to supplementation in terms of particular FAs. Overall, pork LL samples had significantly higher eicosapentaenoic acid (p<0.001) and DHA concentrations (p<0.001), and higher omega-3 (n-3) FAs (p<0.001), as well as an increased omega3:omega6 (n-3:n-6) ratio (p = 0.001). For backfat, supplementation resulted in significantly higher amounts of DHA (p<0.001) and n-3 FAs (p<0.001). Conclusion: These results indicate that dietary supplementation with 1% AURA over a 31 day period can increase the FA composition of pork LL and backfat, specifically the DHA, with no major impact on growth performance and carcass traits.

• 동아시아식생활학회지
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• 제24권6호
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• pp.739-747
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• 2014

본 연구는 인체유방암세포인 MDA-MB-231세포를 이용하여 IDE를 처리하였을 때 세포사멸에 미치는 영향을 세포화학적 방법으로 확인하였다. IDE를 각각 0, 20, 30 및 $40{\mu}g/mL$ 첨가하여 24시간 배양한 후, 세포증식 억제, 막투과성, 세포내 ROS 분석 및 세포사멸 단계의 특성을 FACS 분석하고, RT-PCR에 의한 사멸관련 유전자 중 Bax/Bcl-2 ratio 분석을 통하여 IDE의 항암작용의 조절작용을 밝히고자 하였다. MTT의 세포 증식 억제는 첨가된 IDE의 농도에 따라 유의적으로 감소하였다(p<0.05). 이와 동시에, trypan blue에 대한 염색성과 DCF-DA 형광 분석의 결과는 각각, 막투과성과 세포내 ROS 농도가 모두 농도 의존적으로 증가됨을 보였다(p<0.05). 이와 같은 IDE 농도에 따른 세포화학적 변화 중에서 세포의 초기사멸에서 후기사멸 과정으로 급격한 사멸단계의 변화가 특히, IDE 농도 30과 $40{\mu}g/mL$에서 일어났다. RT-PCR 분석에 의한 Bax/Bcl-2 ratio도 IDE 농도 30과 $40{\mu}g/mL$에서 급격히 증가하였다(p<0.05). 이와 같은 세포화학적 결과와 RT-PCR 결과를 종합해 볼 때, IDE의 유방암세포(MDA-MB-231)에 대한 세포사멸작용은 막 투과성의 증가와 ROS 증가를 통하여 세포에 점진적인 손상을 일으키며, 이는 세포의 생화학적 변화도 초래하여 세포 증식 억제를 감소시켜, 결국 세포내의 사멸관련 유전자 지표인 Bax/Bcl-2 ratio를 크게 변화시키는 일련의 세포사멸을 점진적으로 유도시켜 항유방암의 효과의 가능성을 제시하였다.

• Journal of Nutrition and Health
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• 제27권1호
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• pp.46-52
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• 1994

Recently it is reported that RBC membrane fluidity decreases and RBC calcium levels increase with age. The aim of this study was to analyze changes in lipid and calcium metabolism with age, and to seek relationship of diet and metabolism. With clinically normal Korean adults(male 60, female 63), this study was carried out in three phases : 1) to analyze fatty acid percentage of RBC membrane, 2) to analyze calcium levels of RBC with age, and 3) to compare the effects of dietary fatty acid intake on blood fatty acid profiles. The results are as follows : The P/S ratio of RBC membrane fatty acid decreased with age. The RBC calcium content increased according to age, with women having a higher level than men. The higher intake groups of linolenic acid(C18:3) has statistically higher serum linolenic acid levels. But dietary effects of membrane fatty acid were not found. Therefore, the further research to seek the possible relationship of diet and membrane fatty acid should be continued.

• Journal of Animal Science and Technology
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• 제58권3호
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• pp.12.1-12.7
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• 2016

Background: The objective of this experiment was to investigate the effects of dietary supplementation of rice bran oil (RBO) on growth performance, blood parameter, and immune response in broiler chickens. Methods: A total of 240 1-d-old ROSS 308 male broilers were randomly allotted to 4 dietary treatments with six replicated pens consisting of ten chicks. The basal diet was formulated to be adequate in energy and nutrients. Three additional diets were prepared by adding 5, 10 or 20 g/kg of RBO to the basal diet. The experimental diets were fed on an ad libitum basis to the birds during 35 d. Results: Results indicated that increasing inclusion level of RBO in diets improved BW gain (linear and quadratic, P < 0.01), improve feed conversion ratio (linear, P < 0.05) of birds during 0 to 35 d. There was no effect of inclusion level of RBO in diets on feed intake of birds. There was no effect of inclusion level of RBO in diets on erythrocytes of birds. However, heterophil, lymphocyte, and monocytes increased (linear and quadratic, P < 0.01) with inclusion level of RBO in diets increased. Feeding the diets containing increasing amount of RBO to birds increased (linear, P < 0.01) the concentrations of total cholesterol. Increasing inclusion level of RBO in diets increased concentrations of IgG (linear, P < 0.01). There was no effect of inclusion level of RBO in diets on concentrations of IgM. Conclusions: These results suggest that dietary RBO may be used functional ingredient to improve growth performance, total cholesterol in serum, and immune response of birds.

• 대한지역사회영양학회지
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• 제26권1호
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• pp.12-22
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• 2021

Objectives: This study examined the association between the dietary behavior and weight status of preschool children and their mothers in Daejeon, Korea. Methods: A total of 301 mother-preschool children (aged 3~6 years) dyads were included in this study. The dietary behavior of children and their mothers was assessed using the Nutrition Quotient (NQ) questionnaire for preschoolers and adults, respectively. The NQ questionnaires were completed by the mothers. The overweight/obesity status of children and their mothers was determined using data on height and body weight reported by the mothers. Multiple logistic regression was performed to examine the relationship between the dietary behavior and weight status of children and their mothers. Results: The mean NQ score was 58.9 ± 9.7 in children and 55.6 ± 9.2 in mothers. The NQ score was higher in boys than girls but did not vary by age. The prevalence of overweight/obesity was 27.5% in children and 46.5% in mothers. The physical activity level of mothers and their NQ scores were positively associated with the NQ scores of the children. After adjustment for covariates, the mothers in the highest tertile of NQ scores showed a lower odds ratio (OR) for the unhealthy dietary behavior of children (OR = 0.24, 95% CI = 0.11~0.53, P < 0.001) compared to those in the lowest tertile. The obese mothers showed a higher OR for children's overweight/obesity (OR = 3.38, 95% CI = 1.68~6.80, P = 0.001) compared to normal weight mothers. Conclusions: The dietary behavior and weight status of young children and their mothers were closely linked. Nutrition education programs targeting mothers are necessary for improving maternal and child nutrition. Specifically, these programs need to be tailored to the socioeconomic characteristics or weight status of mothers.

• 한국가금학회지
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• 제21권3호
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• pp.183-193
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• 1994

White Leghorn hens were fed a commercial formula feeds as a basal diet, which was supplemented with 3 types of plant oil safflower, evening primrose and pine seed, at the 10% level for 3 weeks. No significant changes was found between the basal diet and the oil feeding trials in the egg yolk cholesterol content, the laying rate, the egg weight, and the yolk weight, except the feed intake. In the fatty acid composition of the egg yolk lipid, however, the plant oil feedings to hens resulted in considerable increase of $C_{18:2}$ acid with a simultaneous decrease of $C_{18:2}$ acid ,and, consequently, followed by the improvement of balance with P /S and P /M /S ratio close to 1.0 and 1:1:1, which is known a desirable ratio for human health in lipid nutrition, respectively.

• 대한지역사회영양학회지
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• 제29권4호
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• pp.253-264
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• 2024

Objectives: This study investigated the association between eating habits and the utilization of dietary supplements (DSs) according to food-related lifestyle (FRL) among Korean adults. Methods: This study included a total of 300 participants (150 men and 150 women) in their 20s to 60s living in Seoul and Gyeonggi Province. We identified two groups by factor and cluster analysis: an 'active pursuit' group and a 'passive pursuit' group. Differences in eating habits and DS utilization between the two groups were analyzed by chi-square test and t-test. Logistic regression analysis was used to analyze the effect of variables on DS consumption according to FRL. Results: There were significant differences between the two groups in terms of age, alcohol drinking frequency, total dietary score, change in DS consumption after coronavirus disease 2019, and current DS consumption (P < 0.05). The proportion who perceived many health benefits of DSs was higher in the 'active pursuit' group than in the 'passive pursuit' group (P = 0.003). The most commonly consumed type of DSs was multivitamins & minerals for the 'active pursuit' group, and omega-3 fatty acids for the 'passive pursuit' group. The 'an active pursuit' group consumed DSs 2.93 times more (95% confidence interval [CI]: 1.44-5.97) compared to the 'passive pursuit' group, after adjusting for confounders. In the 'active pursuit' group, the health pursuit (odds ratio [OR] = 6.54, 95% CI: 1.44-29.61) and rational consumption pursuit factors (OR = 0.26, 95% CI: 0.08-0.83) were associated with DS consumption, whereas only the health pursuit factor had a significant association (OR = 5.37, 95% CI: 2.08-13.88) within the 'passive pursuit' group. However, total dietary score and DSs consumption did not show a relationship. Conclusions: By understanding the consumption characteristics of DSs according to FRL, this can serve as basic data necessary for promoting health through the utilization of DSs and healthy behaviors.

• Asian-Australasian Journal of Animal Sciences
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• 제14권4호
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• pp.525-534
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• 2001

A total of 120 pigs were used to investigate the effects of yucca extracts on the growth performance, nutrient digestibility, nutrient excretion and carcass characteristics of finishing pigs fed different levels of dietary protein. Pigs were allotted into $2{\times}3$ factorial design by the supplementation of yucca extracts (YE, 0 and 120 mg/kg) and 3 levels of dietary protein (14, 16, 18% for early finisher and 12, 14, 16% for late finisher for low, medium and high protein diet, respectively). During the early finishing period (51~76 kg BW), no significant difference was found in growth performance regardless of the YE supplementation or dietary protein levels. Growth performance of late finishing pigs (76~101 kg BW) was also not significantly different among treatments. However, ADG of pigs fed YE diet was significantly improved (p<0.05) regardless of the dietary protein levels. For the overall period (51~101 kg BW), although adding YE to the diet and elevating the protein level showed better ADG, there were no significant differences on growth performance among treatments. Early finishers showed significantly higher crude protein, crude ash and crude fat digestibilities when they were fed diets supplemented with YE. Digestibilities of amino acids were not affected by YE. Late finishers did not show any significant differences in proximate nutrient digestibilities regardless of YE supplementation or dietary protein levels. YE tended to slightly improve the CP digestibility, however no significant difference was found with increased dietary protein levels. There was no significant difference in amino acid digestibilities with YE supplementation or dietary CP levels during the late finishing period. Dry matter (DM) and nitrogen (N) excretion in feces did not show any significant difference among treatments. Early finishing pigs also did not respond to the inclusion of YE or dietary protein levels (p<0.05). Fecal N excretion of early finishing pigs seemed to be lowered in pigs fed YE. Pigs fed medium dietary protein diet tended to excrete a higher amount of N during the early finishing period, but not statistically different. A slight increase in fecal N excretion was found with the increased level of dietary protein during the late finishing period. For ammonia nitrogen excretion, although there was no significance, the NH3-N content tended to be increased by the increased dietary protein levels and with YE supplementation. The NH3-N content in manure increased by 24.5% with YE supplementation. There were no significant differences in carcass weight, backfat thickness, carcass grade and loin eye area among treatments. However, pigs fed non-YE with low protein diet showed a significantly (p<0.05) low carcass ratio among treatments and there was significant (p<0.05) difference between the YE-added treatment and non YE treatment in carcass ratio. As for the feed cost, the cost of feeding high level protein was higher than that of medium level protein by 5% and low level protein by 9% (p<0.05). Therefore, based on this study, it could be concluded that environmentally friendly agents might play a role to some extent in finishing pigs from the aspect of pollution control, and that more than 14 and 12% of dietary protein for early finishing and late finishing pigs respectively do not necessarily guarantee high growth performance.

• Asian-Australasian Journal of Animal Sciences
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• 제23권8호
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• pp.1073-1079
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• 2010

Forty-eight ((Duroc${\times}$Yorkshire)${\times}$Landrace) pigs with an average initial body weight (BW) of $48.47{\pm}1.13\;kg$ were used in a 12-week growth trial to investigate the influence of Anion (silicate) supplementation on growth performance, nutrient digestibility, meat quality and fecal noxious gas content in growing-finishing pigs. Pigs were allotted into three dietary treatments in a randomized complete block design according to sex and initial BW. Each dietary treatment consisted of four replications with four pigs per pen. Dietary treatments included: i) CON (basal diet), ii) HCI (basal diet+3 g/kg Anion), iii) HCII (basal diet+6 g/kg Anion). No significant difference (p>0.05) was detected for average daily gain (ADG), average daily feed intake (ADFI) and gain/feed ratio (G/F) throughout the experiment, although dietary supplementation of Anion numerically increased these characteristics compared with CON. The dietary HCI group significantly (p<0.05) increased the coefficient of total tract apparent digestibility (CTTAD) of dry matter (DM), nitrogen (N) and energy compared with the CON group (p<0.05). No significant difference was observed in meat quality except that meat firmness was linearly (p<0.05) increased by the Anion supplementation, while an increased tendency in meat color and a decreased tendency in 2-thiobarbituric acid reactive substances (TBARS) was also observed (p<0.10). Anion supplementation linearly (p<0.05) decreased the fecal $NH_3$ compared with the CON group. However, dietary Anion supplementation at 3 g/kg decreased the $H_2S$ concentration compared with CON, while no significant difference was detected in the HCII group, although the $H_2S$ emission was numerically decreased compared with CON. In conclusion, supplementation of the diet with 3 g/kg Anion was found to exert a beneficial effect on nutrient digestibility and meat quality of growing-finishing pigs, and concomitantly decreased the noxious gas emission without negative effect on growth performance.

• 동아시아식생활학회지
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• 제22권6호
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• pp.826-835
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• 2012

This study was conducted to examine the quality characteristics of Oncorhynchus masou stock containing various amounts of tomato (2%, 4%, 6%, 8% and 10%). To accomplish this, the moisture contents, Hunter's color value, pH, salinity, sugar contents, free sugar contents, minerals and free amino acid were measured. The b value decreased, while the L value, a value, pH, salinity, sugar contents, total sugar (fructose, glucose, sucrose) and minerals (Na, Mg, K, Ca, Fe) increased as the ratio of tomato increased. A total of 33 free amino acids were detected, and the amino acid content increased as the ratio of tomato increased. Investigation of different attributes revealed that tomato content significantly affected color intensity, savory flavor and tomato taste. In the acceptance test, stock containg 8% tomato was preferred for flavor, taste, texture and overall quality ; therefore, this was taken as the optimal tomato content for maximizing the overall quality of Oncorhynchus masou stock.