• Title/Summary/Keyword: diagnostic key

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Tachinus (Tachinus ) javanus Cameron (Coleoptera: Staphylinidae: Tachyporinae) New to Korea with a Key to the Known Species of the Genus Tachinus Gravenhorst (한반도 미기록 붉은뾰족반날개(딱정벌레목: 반날개과: 뾰족반날개아과)의 보고 및 북방뾰족반날개속의 종 검색표)

  • Jeong, Woo-Jin;Ahn, Kee-Jeong
    • Korean journal of applied entomology
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    • v.57 no.1
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    • pp.1-5
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    • 2018
  • Tachinus (Tachinus) javanus Cameron is identified for the first time in the Korean Peninsula. Description, illustrations of habitus and diagnostic characters, and a key to the known species of Korean Tachinus Gravenhorst are provided. Tachinus (Tachinus) yezoensis Li is redescribed with male characteristics.

First Record of the Subfamily Conganteoninae Olmi (Hymenoptera: Dryinidae) from South Korea (한국산 미기록아과 Conganteoninae Olmi (벌목: 집게벌과)에 대한 보고)

  • Kim, Chang-Jun;Park, Bia;Lee, Jong-Wook
    • Korean journal of applied entomology
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    • v.54 no.3
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    • pp.275-279
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    • 2015
  • The subfamily Conganteoninae Olmi, 1984 belonging to the family Dryinidae is reported for the first time in South Korea based on the discovery of Conganteon nepalense Olmi, 1984 and Fiorianteon junonium Olmi, 1984. A key, diagnosis, illustrations and photographs of the diagnostic characteristics are provided.

New Records of Aphelinids, Encarsia Förster (Hymenoptera: Aphelinidae), in Korea (우리나라 Encarsia Förster에 대한 보고(벌목: 면충좀벌과))

  • Suh, Soo-Jung;Evans, Gregory A.
    • Korean journal of applied entomology
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    • v.54 no.3
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    • pp.281-284
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    • 2015
  • Two species of Encarsia, Encarsia explorata (Silvestri) from Aulacaspis rosarum Borchsenius, A. yabunikkei Kuwana and Lepidosaphes pinnaeformis ($Bouch\acute{e}$), and Encarsia lutea (Masi) from Bemisia tabaci (Gennadius) are newly documented in the Korean fauna of aphelinids (Hymenoptera: Aphelinidae). Brief diagnostic criteria, photographs, and information on the distribution and hosts of these species are provided. Also a key to species of Encarsia from Korea is given for correct species identification.

An Additional Species of Leucospis (Hymenoptera: Chalcidoidea: Leucospidae), New to South Korea (한국산 밑들이벌속(벌목: 좀벌상과: 밑들이벌과)의 미기록 1종 보고)

  • Kim, Chang-Jun;Lim, Jongok;Kim, Il-Kwon
    • Korean journal of applied entomology
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    • v.56 no.4
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    • pp.339-343
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    • 2017
  • Korean species of Leucospis Fabricius (Hymenoptera: Chalcidoidea: Leucospidae) are reviewed, with one newly-recognized species, Leucospis sinensis Walker, 1860. An identification key to the Korean Leucospis species, diagnoses, biological information and photographs of diagnostic characters are provided in the present study.

Discovery of an Aquatic Wasp, Agriotypus silvestris Konishi & Aoyagi (Hymenoptera: Ichneumonidae: Agriotypinae) in South Korea (한국산 물벌속(벌목: 맵시벌과: 물벌아과)의 미기록 1종 보고)

  • Kim, Chang-Jun;Oh, Seung Hwan;Suh, Kyong-In
    • Korean journal of applied entomology
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    • v.57 no.4
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    • pp.275-278
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    • 2018
  • An aquatic wasp, Agriotypus silvestris Konishi & Aoyagi, 1994 is newly recognized in South Korea. A diagnosis and photographs of the diagnostic characters for the species and an identification key to the South Korean Agriotypus species are provided herein.

Tetratoma (Abstrulia) pictipennis New to South Korea (Coleoptera: Tetratomidae), with a Key to Species of the Subgenus Abstrulia

  • Lee, Seung-Gyu;Jung, Sang-Woo;Kim, Yoon-Ho
    • Animal Systematics, Evolution and Diversity
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    • v.38 no.2
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    • pp.65-68
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    • 2022
  • The subgenus Abstrulia Casey belonging to the genus Tetratoma Fabricius of the family Tetratomidae is distributed ten species over the Holarctic region, including eight Palaearctic species. In East Asia, four species from Russian Far East and two species from Japan have been recorded, respectively. In the Korean Peninsula, two species, T. (A.) ainu (Nakane) and T. (A.) pictipennis Reitter, were reported up to date, and latter species is newly recorded in South Korea in this paper. A redescription, habitus photograph, and illustrations of diagnostic characters of the species are provided, with a key to species of the subgenus Abstrulia.

Codon Optimization, Soluble Expression and Purification of PE_PGRS45 Gene from Mycobacterium tuberculosis and Preparation of Its Polyclonal Antibody Protein

  • Xu, Tao;Li, Minying;Wang, Chutong;Yuan, Meili;Chang, Xianyou;Qian, Zhongqing;Li, Baiqing;Sun, Meiqun;Wang, Hongtao
    • Journal of Microbiology and Biotechnology
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    • v.31 no.11
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    • pp.1583-1590
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    • 2021
  • Studies have demonstrated that PE_PGRS45 is constitutively expressed under various environmental conditions (such as nutrient depletion, hypoxia, and low pH) of the in vitro growth conditions examined, indicating that PE_PGRS45 protein is critical to the basic functions of Mycobacterium tuberculosis. However, there are few reports about the biochemical function and pathogenic mechanism of PE_PGRS45 protein. The fact that this M. tuberculosis gene is not easily expressed in E. coli may be mainly due to the high content of G+C and the use of unique codons. Fusion tags are indispensable tools used to improve the soluble expression of recombinant proteins and accelerate the characterization of protein structure and function. In the present study, His6, Trx, and His6-MBP were used as fusion tags, but only MBP-PE_PGRS45 was expressed solubly. The purification using His6-MBP tag-specific binding to the Ni column was easy to separate after the tag cleavage. We used the purified PE_PGRS45 to immunize New Zealand rabbits and obtained anti-PE_PGRS45 serum. We found that the titer of polyclonal antibodies against PE_PGR45 was higher than 1:256000. The result shows that purified PE_PGRS45 can induce New Zealand rabbits to produce high-titer antibodies. In conclusion, the recombinant protein PE_PGRS45 was successfully expressed in E. coli and specific antiserum was prepared, which will be followed by further evaluation of these specific antigens to develop highly sensitive and specific diagnostic tests for tuberculosis.

Mitochondrial defect-responsive gene signature in liver-cancer progression

  • Lee, Young-Kyoung;Woo, Hyun Goo;Yoon, Gyesoon
    • BMB Reports
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    • v.48 no.11
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    • pp.597-598
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    • 2015
  • Mitochondrial respiratory defect is a key bioenergetics feature of hepatocellular carcinoma (HCC) cells. However, their involvement and roles in HCC development and progression remain unclear. Recently, we identified 10 common mitochondrial defect (CMD) signature genes that may be induced by retrograde signaling-mediated transcriptional reprogramming in response to HCC mitochondrial defects. HCC patients with enriched expression of these genes had poor prognostic outcomes, such as shorter periods of overall survival and recurrence-free survival. Nuclear protein 1 (NUPR1), a key transcription regulator, was up-regulated by Ca++-mediated retrograde signaling. NUPR1-centric network analysis and a biochemical promoter-binding assay demonstrated that granulin (GRN) is a key downstream effector of NUPR1 for the regulation of HCC cell invasiveness; association analysis of the NUPR1-GRN pathway supported this conclusion. Mitochondrial respiratory defects and retrograde signaling thus play pivotal roles in HCC progression, highlighting the potential of the NUPR1-GRN axis as a novel diagnostic marker and therapeutic target for HCC.

A taxonomic Study of Lysimachia sect. Spicatae (Primulaceae) Based on Morphological Characters (형태학적 형질에 의한 까치수염속(Lysimachia) Spicatae절 식물의 분류학적 연구)

  • Kim, Jin Man;Tae, Kyoung-Hwan;Kim, Joo-Hwan
    • Korean Journal of Plant Taxonomy
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    • v.37 no.1
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    • pp.61-78
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    • 2007
  • The general morphological characters of Lysimchia sect. Spicatae were reviewed. From the results, numerical analyses were conducted based on 34 morphological characters from 14 populations of three species in Korea in order to investigate the key characters among the treated taxa and taxonomic delimitation and to discuss the systematic relationship. Habitat, leaf apices, presence or absent of hair on leaves, stipules, stems and bracts, branch of stems, kind of hair on floral axis and pedicels, shape of florescens were important diagnostic characters to identify the species. The relationship between Lysimachia clethoides and L. barystachys was more close than that of L. fortunei based on the quantitative characters. Also, a new key was made up from the result.

Morphology and phytogeography of Laminaria appressirhiza and L. inclinatorhiza (Phaeophyceae) from the Sea of Okhotsk

  • Klochkova, Tatyana A.;Kim, Gwang-Hoon;Belij, Mihail N.;Klochkova, Nina G.
    • ALGAE
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    • v.27 no.3
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    • pp.139-153
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    • 2012
  • The re-examination of morphological and anatomical characters of Laminaria appressirhiza and L. inclinatorhiza collected from different localities in the Sea of Okhotsk was performed. Despite their commercial and ecological importance to the region they have not been comprehensively reviewed since their first description in 1970. Our results show that some original diagnostic key characters (e.g., shape of holdfast, shape of sporangial sori, and dissection of blade) are not stable and have deviations from the type morphology when plants grow in different environments. In L. inclinatorhiza, the sporangial sori development occurred differently to the pattern indicated in original species description as they did not develop simultaneously on both sides of the blade. Instead, the sporangial sori outlines on both sides of the blade did not coincide at first and only became coincident later. Also, a deep-water population of L. inclinatorhiza with an unusual and interesting morphology, growing at depths of 15-25 m on opened rocky coasts in Taujskaya Bay (northern part of the Sea of Okhotsk) was found. The stable diagnostic key characters to distinguish these two species are the cone-like, multilayered, very thick and massive holdfast (in L. inclinatorhiza) and rolled margins of blades, lamellar rosette-like part of thallus, and sporangial sori developing only on one side of the blade (in L. appressirhiza). The ecological characteristics, distribution, and abundance of both species in the Sea of Okhotsk are discussed. Both species are perennial and widely distributed in the region. L. appressirhiza is more often found as a subdominant species among other kelps, forming maximum biomass and density of 7-9 kg and 8-25 plants per $1m^2$, respectively. L. inclinatorhiza sometimes forms local mono-species communities with maximum biomass and density of 10-12 kg and 10-15 plants per $1m^2$, respectively.