• 대한한방내과학회지
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• 제39권6호
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• pp.1181-1190
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• 2018

Objective: This study investigated the effect of purified medical herb extracts such as Alisma canaliculatum and Polyporus umbellatuson adipogenic differentiation of human bone marrow derived mesenchymal stromal stem cells (hBMSCs). Methods: Two different medical herb were extracted using hot distilled water. The optimal concentration of extracts were fixed at 100 ng/ml by means of cell viability and cytotoxic assay. To test the adipogenic differentiation ability of extracts, we induced the adipogenesis of hBMSCs for 21 days. At day 5, the cell was harvested to check mRNA and protein expression level of adipogenic related factors. The efficacy of lipid droplet formation was evaluated using the oil-red O staining method at days 21. Results: Two different medical herb extracts have no toxicity on hBMSCs. And two different medical herb extracts significantly decreased formation of lipid droplet compared with control groups in hBMSCs. The A. canaliculatum extract group showed the lowest mRNA and protein expression level of adipossgenic related transcription factors. This data suggests that extract of A. canaliculatum and P. umbellata decrease the adipogenic differentiation of hBMSCs. Conclusions: Our findings indicate that water-extract of A. canaliculatum and P. umbellata will be useful therapeutic reagents for prevention of obesity related disease such as diabetes, hyperlipidemia, coronary artery disease, and osteoporosis.

• 셀메드
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• 제9권1호
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• pp.1.1-1.13
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• 2019

Background: Contents of bioactive substances extractable from different parts of terrestrial plants vary enormously. Aim: To ascertain that parts of Withania somnifera other than its roots can also be used for prevention and cure of unavoidable stress triggered central hypersensitivity to pain. Material and Methods: Groups of male or female mice treated either with Withania somnifera extracts or with metformin, aspirin, imipramine, diazepam and niacin for 11 consecutive days were subjected to "foot-shock stress-induced hyperthermia" and "hot plate" tests on the 1st, 5th, 7th, and 10th days of the experiments. On the 11th day, they were subjected to tail suspension test and on 12th day pentobarbital hypnosis test. Results: Except for diazepam and imipramine, protective effects of all other tested drugs as well as of the Withania somnifera extracts against stress-induced central hypersensitivity to pain were accompanied by their preventive effects against foot-shock stress-induced body weight losses. All observed stress response suppressing effects of all test agents increased with increasing numbers of treatment days. However, mean duration of pentobarbital-induced sleep was shorter in the extracts treated groups and longer in the diazepam treated ones only. Conclusions: Reported observations reveal that pharmacological activity profile of Withania somnifera extracts in male and female mice are almost identical, and are not like those of several drugs currently often prescribed for the treatment of diabetes-associated comorbidities. Withanolides are not the only extractable bioactive constituents of Withania somnifera. The described bioassay system is well suited for pharmacological standardization of diverse types of Withania somnifera extracts.

• Biomolecules & Therapeutics
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• 제27권1호
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• pp.107-116
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• 2019

The global obesity epidemic and associated metabolic diseases require alternative biological targets for new therapeutic strategies. In this study, we show that a phytochemical sulfuretin suppressed adipocyte differentiation of preadipocytes and administration of sulfuretin to high fat diet-fed obese mice prevented obesity and increased insulin sensitivity. These effects were associated with a suppressed expression of inflammatory markers, induced expression of adiponectin, and increased levels of phosphorylated ERK and AKT. To elucidate the molecular mechanism of sulfuretin in adipocytes, we performed microarray analysis and identified activating transcription factor 3 (Atf3) as a sulfuretin-responsive gene. Sulfuretin elevated Atf3 mRNA and protein levels in white adipose tissue and adipocytes. Consistently, deficiency of Atf3 promoted lipid accumulation and the expression of adipocyte markers. Sulfuretin's but not resveratrol's anti-adipogenic effects were diminished in Atf3 deficient cells, indicating that Atf3 is an essential factor in the effects of sulfuretin. These results highlight the usefulness of sulfuretin as a new anti-obesity intervention for the prevention of obesity and its associated metabolic diseases.

• 융합정보논문지
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• 제9권8호
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• pp.248-253
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• 2019

노인성 치매의 전 임상단계인 경도인지장애(MCI)를 조기 진단하고, 조기 개입한다면, 치매의 발병률을 줄일 수 있다. 본 연구는 우리나라 지역사회 노인의 MCI 예측 모형을 개발하고 노년기 인지장애의 예방을 위한 기초자료를 제공하였다. 연구대상은 2012년 Korean Longitudinal Survey of Aging(KLoSA)에 참여한 65세 이상 지역사회 노인 3,240명(남성 1,502명, 여성 1,738명)이다. 결과변수는 MCI유병으로 정의하였고, 설명변수는 성, 연령, 혼인상태, 교육수준, 소득수준, 흡연, 음주, 주1회 이상의 정기적인 운동, 월평균 사회활동 참여시간, 주관적 건강, 고혈압, 당뇨병을 포함하였다. 예측모형의 개발은 Restricted Boltzmann Machine(RBM) 인공신경망을 이용하였다. RMB 인공신경망을 이용하여 우리나라 지역사회 노인의 MCI 예측 모형을 구축한 결과, 유의미한 요인은 연령, 성별, 최종학력, 주관적 건강, 혼인상태, 소득수준, 흡연, 규칙적 운동이었다. 이 결과를 기초로 MCI 고위험군의 특성을 고려한 맞춤형 치매 예방 프로그램의 개발이 요구된다.

• 응용통계연구
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• 제32권4호
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• pp.619-630
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• 2019

이상지질혈증은 한국인의 대표적인 성인병이며 지속적인 관리가 필요한 만성질환이다. 또한 고혈압이나 당뇨병과 함께 심혈관계 질환의 위험 요인으로 잘 알려져 있다. 하지만 혈관 질환은 검사 없이는 질병 판단을 하기 어려운 것이 현실이다. 본 연구에서는 이상지질혈증의 인지와 예방을 위하여 관련된 위험 요인을 확인한다. 이들을 종합하여 시각화하면서 발병률 예측까지 가능한 통계적 도구 노모그램을 구축하였다. 데이터는 국민건강영양조사 6기, 7기 제1차년도 (2013-2016) 데이터를 사용하였다. 분석 순서로는 먼저 이상지질혈증의 총 12가지 위험 요인을 교차분석을 통해 확인하였다. 그리고 순수 베이지안 분류기를 이용하여 이상지질혈증에 대한 모형으로 노모그램을 구축하였다. 구축한 노모그램은 ROC 곡선과 Calibration plot을 사용하여 신뢰성을 검증하였다. 마지막으로 이전에 제시했던 로지스틱 노모그램과 본 연구에서 제안한 베이지안 노모그램을 비교하였다.

• 보건행정학회지
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• 제31권3호
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• pp.292-300
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• 2021

Background: Medication adherence in hypertension is the most important to control blood pressure and prevent major complications. The purpose of this study was to identify factors affecting medication adherence and to examine the relationship between medication adherence and blood pressure control in Korea. Methods: This study used data from the 7th Korea national health and nutrition examination survey (2016-2018) of the Korea Disease Control and Prevention Agency. We selected 4,063 hypertensive patients from the data. And we choose socio-demographic, health behavior, healthcare utilization, and severity characteristics as hypertensive patient characteristics. Results: Of the patients with hypertension, 92.3% had shown adherence to medication as of 2016-2018 and shows variation according to the characteristic of patients. The cases with male, under 50 years old, urban area, single household, unmet medical services, less than 5 years of hypertension duration, no comorbidities (diabetes mellitus, myocardial infarction) showed significantly low medication adherence. After adjusting for confounders, adherent patients tended to have lower current systolic blood pressure (β=-10.846, p<0.001) and diastolic blood pressure (β=-5.018, p<0.001) than nonadherent patients. And, adherent patients increased the control odds of blood pressure compared with nonadherent patients (odds ratio, 3.02; 95% confidence interval, 2.21-4.12). Conclusion: This study confirmed that adherence to antihypertensive drugs was effective in controlling blood pressure. In order to more actively manage hypertensive patients at the national level, it is necessary to make an effort to improve the medication compliance of nonadherent groups, such as early-diagnosis patients, young patients under 50 years of age, and patients living alone.

• BMB Reports
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• 제54권2호
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• pp.124-129
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• 2021

In current times, obesity is a major health problem closely associated with metabolic disease such as diabetes, dyslipidemia, and cardiovascular disease. The direct cause of obesity is known as an abnormal increase in fat cell size and the adipocyte pool. Hyperplasia, the increase in number of adipocytes, results from adipogenesis in which preadipocytes differentiate into mature adipocytes. Adipogenesis is regulated by local and systemic cues that alter transduction pathways and subsequent control of adipogenic transcription factors. Therefore, the regulation of adipogenesis is an important target for preventing obesity. Myonectin, a member of the CTRP family, is a type of myokine released by skeletal muscle cells. Although several studies have shown that myonectin is associated with lipid metabolism, the role of myonectin during adipogenesis is not known. Here, we demonstrate the role of myonectin during adipocyte differentiation of 3T3-L1 cells. We found that myonectin inhibits the adipogenesis of 3T3-L1 preadipocytes with a reduction in the expression of adipogenic transcription factors such as C/EBPα, β and PPARγ. Furthermore, we show that myonectin has an inhibitory effect on adipogenesis through the regulation of the p38 MAPK pathway and CHOP. These findings suggest that myonectin may be a novel therapeutic target for the prevention of obesity.

• 지역사회간호학회지
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• 제31권spc호
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• pp.534-548
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• 2020

Purpose: This study was conducted to evaluate the prevalence and lifestyle habits of cardiovascular disease (CVD) according to the type of disability in Korean adults compared to adults without disability. Methods: This study was secondary data analysis using the National Health check-up database from 2010 to 2013. Among the total 395,627 adults aged 30~80, the physically disabled (n=21,614) and the mentally disabled (n=1,448) who met the diagnosis criteria were extracted and compared with non-disabled (n=372,565) through 1:2 propensity score matching for nine characteristics. Results: Prior to matching, the prevalence of CVD was 34.4% in individuals without disabilities, accounting for 53.8% in those with physical disabilities and 22.4% in those with mental disabilities, showing significant differences between groups (p<.001). After matching, compared to the individuals without disability, those with physically disabled had significantly higher prevalence of CVD and the average number of CVD (p<.001). The prevalence of hypertension, diabetes, and vascular disease was significantly higher in the physically disabled (p<.05). Drinking was significantly higher in the non-disabled than in the physically and mentally disabled, and smoking was more in the non-disabled than in the mentally disabled. Physical activity was found to be significantly less in both the physically and mentally disabled than in the non-disabled (p<.01). Conclusion: It is necessary to confirm the differences in the prevalence of CVD risk factors and lifestyle according to the type of disability, suggesting the development and verification of health promotion programs including physical activity for CVD prevention in the disabled with CVD risk factors.

• 정보처리학회논문지:컴퓨터 및 통신 시스템
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• 제11권3호
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• pp.83-90
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• 2022

당뇨병은 오늘날 주변에서 흔히 찾아볼 수 있는 질병이며, 당뇨병성 족부 궤양(당뇨발)이라는 심각한 합병증으로 발전하는 사례 또한 많이 나타난다. 따라서 이를 사전에 진단하고 예방하는 것은 중요한 과제이며 본 논문에서 그 방안을 제시한다. 본문에서 소개하는 기존의 연구들을 바탕으로 발의 압력과 온도 정보는 당뇨발과 깊은 상관관계가 있음을 알 수 있으며, 해당 지표들을 측정하는 IoT 기기인 스마틴솔을 개발과정 및 아키텍쳐를 소개한다. 또한, 더 나아가 스마틴솔로 측정한 데이터들의 실제 당뇨발 진단을 위한 AI 분석 전처리 과정을 기술하며, 측정된 압력 그래프와 실제 사람의 발걸음 분포의 비교 등을 통해 실시간으로 수집하는 다중 정보들이 기존의 IoT 기기들보다 효율적이고 신뢰성 있다는 결과를 제시한다.

• Journal of Interdisciplinary Genomics
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• 제4권2호
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• pp.35-38
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• 2022

Background: Prader-Willi syndrome (PWS) is a complex genetic disease associated with growth impairment, severe obesity and metabolic dysfunctions. High proportion of PWS patients are born small for gestational age (SGA) than normal children, which also increase the risk of growth impairment and metabolic dysfunction in PWS. We aimed to compare growth outcome and metabolic profiles between SGA and appropriate for gestational age (AGA) PWS patients. Methods: Data of 55 PWS children and adults aged more than 2 years old (32 male and 23 female, age 2-18.8 years) from single center were studied. Only patients who were treated with GH were included. The clinical characteristics and laboratory findings were reviewed retrospectively. Results: Among 55 subjects, 39 had 15q11-13 deletion and 16 had uniparental disomy (UPD). Twenty (36.3%) were born SGA. All patients received GH treatment, and 11 (20%) discontinued GH treatment. Mean age at GH treatment initiation was 2.5 (range 0.3-12.4) years, and mean duration of treatment was 6.3 (range 1.0-11.3) years. Current height-SDS (-0.36 vs -0.16) and BMI-SDS (1.44 vs 1.33) did not differ between AGA and SGA group. Two patients in SGA group, but none in AGA group had diabetes mellitus. Mean glucose level was also higher in SGA group (100.1 vs 114.4 mg/dL). Conclusion: Our report gives an overview of growth profile and metabolic dysfunctions recorded in GH treated PWS patients. Growth profile did not differ between AGA and SGA group. Glucose level was higher in SGA group, so more careful monitoring and prevention for DM will be required in SGA group.