• Title/Summary/Keyword: developmental pattern

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Empirical approach to Cognitive Process for Problems of Marine Design (해양디자인 문제해결을 위한 인지적 프로세스에 관한 실증적 접근)

  • Kim, Kiesu
    • The Journal of the Korea Contents Association
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    • v.12 no.12
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    • pp.126-134
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    • 2012
  • The researchers of this study closely looked into the methods for cognitive-scientific approach to problems of marine design at a time when the overall values of marine cultures are acknowledged in full scale. To that end, the researchers analyzed the problems and problem-solving process for the initial approach to marine design. At the same time, the researchers made the matrix of the design-developmental directions by cognitive scientific approach. After selecting the subjects, the researchers collected verbal protocol and behavior protocol which were shown in the process of a designer's thinking. This was for the sake of protocol analysis which is the representative research technique of cognitive science. Based on the collected data, the researchers empirically analyzed the behavior patterns shown in the marine design process so as to develop the design behavior-graph pattern of designers in an objective and systematic way. The behavior graph was helpful for looking into the initial developmental directions of design and for predicting cognitive structure of designers. The researchers hope that this study will become a fundamental material for predicting cognitive directions of designer for planning and designing the marine design.

In Vitro Developments of Porcine Embryos Produced by In Vitro Fertilization and Parthenogenetic Activation (체외수정과 단위발생된 돼지 난자의 체외발달 양상의 비교)

  • Uhm, S.J.;Kim, N.H.;Kim, E.Y.;Kim, M.K.;Cha, S.K.;Yoon, S.H.;Park, S.P.;Chung, K.S.;Lim, J.H.
    • Clinical and Experimental Reproductive Medicine
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    • v.24 no.1
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    • pp.43-49
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    • 1997
  • The objective of this study is to determine developmental pattern and cell allocation to the ICM and TE in haploid and diploid of embryos following parthenogenetic activation and in vitro fertilization. The incidence of development to blastocyst was lower in the combined treatments of ethanol stimulation and cytochalasin B as compared to the control. However, the combined ethanol stimulation and cytochalasin B treatment (diploid) accelerated development to the blastocyst as compared to the ethanol treatment alone (haploid). Significantly reduction in the average number of total cells and ICM was observed in the parthenotes alone as compared to fetilized embryos, but those of combined ethanol stimulation and cytochalasin B treatment embryos were significantly increased as compared to ethanol alone embryos. These results suggested that the ploidy affects preimplantation developmental patters and cell allocation to the ICM and TE in the porcine.

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Effects of Mutagens on the Synthetic Patterns of Proteins During the Early Developmental Stages in Mice (생쥐 초기배아의 단백질 합성양상에 미치는 돌연변이 유발원의 영향)

  • 이양림
    • The Korean Journal of Zoology
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    • v.23 no.3
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    • pp.149-160
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    • 1980
  • The effects of mutagens, MMS and captan, on the patterns of proteins synthesized during the early developmental stages in mice were analyzed using two-dimensional electrophoresis. Three classes of proteins were observed in terms of synthetic pattern during the preimplantation stages. The first class is synthesized from the m-RNA, which was made and preserved throughout oogenesis and activated at the fertilization. The synthesis of these proteins did not seem to be influenced by MMS. The second class, which may be stagespecific proteins synthesized by newly transcribed m-RNA, was selectively inhibited by MMS. The third class, the synthesis of which is also suppressed by MMS, is the proteins synthesized by the m-RNA transcribed in augmented fashion. While MMS inhibits protein synthesis dependent on thenew transcription, this mutagen enhances a synthesis of a few proteins which were not observed in the untreated embryos. Captan did not affect protein synthesis at morula stage.

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Expression of rpr, grim, dcp-1, diapl, and diap2 during Drosophila Development (노랑초파리 발생과정에서 rpr, grim, dcp-1, diapl, diap2의 발현)

  • Park, Ji-Gweon;Chung, Ki-Wha;Kim, Se-Jae
    • Development and Reproduction
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    • v.5 no.2
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    • pp.131-136
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    • 2001
  • The developmental profiles of rpr, grim, dcp-1, diapl, diap2 transcripts, which were involved in programmed cell death, were analyzed using competitive RT-PCR in whole animals during Drosophila development. The fluctuation patterns of transcript levels of the apoptotic initiators(rpr and grim) were similar to those of the ecdysone titer in Drosophila life cycle. The transcript of dcp-1, which is considered as effector caspase, was expressed strong1y at early embryo and female adult stages. However, the transcript levels of anti-apoptotic factors diap1 and diap2, showed the reverse pattern comparing with those of apoptotic factors(rpr and grim). Also, the transcript levels of rpr, diap2 and dcp-1 were quantified in the salivary glands and wing discs dissected from the wandering late third instar larva. The transcript levels of rpr and diap2 were changed reversely each other in both tissues from wandering stage to puparium formation. These results suggest that the expressions of cell death related genes are regulated by the ecdysone signals during normal development.

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Developmental Characteristics and Spatial Distribution of Mango Shield Scale, Protopulvinaria mangiferae (Green) (Homoptera : Coccidae) on Elaeocarpus sylvestris (Lour.) (담팔수깍지벌레의 충태별(蟲態別) 생육(生育) 특성(特性)과 수상분포양식(樹上分布樣式))

  • Kim, Jong-Kuk
    • Journal of Forest and Environmental Science
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    • v.11 no.1
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    • pp.40-49
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    • 1995
  • This study was carried out to investigated the developmental characteristics and spatial distribution of mango shield scale, Protopulvinaria mangiferae injuring to Elaeocarpus sylvestris(Lour.) This mango shield scale is parthenogentic. The oviparous female have three larval stages and adult. This species has two generation a year. The crawlers of the 1st generation emerged from late may to middle July, and those of the 2nd generation from early August to early October. Their emergence reached its peak in early part of the occurrence period. The crawlers disperse from 10 to 14 o'clock from ventral side of the adult and more than 90% had settled with in 8 hours on the leaves of E. sylvestris. The larvae and adults were distributed contagiously between trees. The adult densities were higher in the lower part of the crown than those in the middle or upper parts. But differents in densities were not significant among four directions. The distribution of the adult scales per leaf cluster showed an aggregation with a slightly colonized pattern.

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A Study on Oriental Images of Modern Fashion in the New Paradigm (뉴 패러다임을 통해 나타난 현대 패션 동양적 이미지 연구)

  • Ko, Myung-Sin;Chae, Keum-Seok
    • Journal of the Korean Society of Clothing and Textiles
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    • v.32 no.5
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    • pp.704-716
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    • 2008
  • This study aims to explore the creation of fashion design that embraces the diversity of human culture by examining oriental images that appear in the modern fashion with a focus on new paradigms that significantly affect human life as the latest trends, and by reflecting the trends of the times. The results of this study are as follows: First, this study examined that with regard to paradigm, a theoretical frame to view universe, the dichotomous, determinist, mechanism, linear paradigm collapsed to convert to undetermined, exoteric, pluralistic, indeterministic, organic, and holistic paradigm. The new paradigm is identified to have emerged before and after 1950s, and through the new paradigm, the characteristics of postmodernism such as historicality, popularity, locality, folkways and the characteristics of deconstructionism, internally mutual text, post phenomenon, undeterminability and externally exposure, destruction, poverty, decomposition and analysis, were identified. Second, the Orient is defined as the generic term referring to the entire Asian areas east of Turkey. Through the developmental process of oriental images, it was converted from the Oriental image, which mysteriously and romantically represents oriental elements, into the ethnic image that represents long time oriental traditions and indigenous culture together with the characteristics of the new paradigm. Third, the artistic characteristics of the Oriental costumes presented by Korea, China and Japan are expressed in developmental types, T-type plane structure, layered style, asymmetrical adjustment and easy silhouette, and they show indigenous characteristics of each country, for example, Chinese styles in dragon pattern, red and yellow, Japanese styles in flower design and achromatic color. Fourth, the Oriental Image, combined with the postmodernism and deconstructionism through the new paradigm, has two: one Oriental image highlights traditional elements by creating new Oriental image such as natural image, folk image, hybrid image and deconstruction image; and another is undetermined and vague by combining or decomposing Oriental or Western elements. It is expected that fashion designs that reflect these contexts of the times will contribute to the strengthening of international competitiveness.

Expression Pattern of Early Growth Response Gene 1 during Olive Flounder (Paralichthys olivaceus) Embryonic Development

  • Yang, Hyun;Lee, Jeong-Ho;Noh, Jae Koo;Kim, Hyun Chul;Park, Choul-Ji;Park, Jong-Won;Kim, Kyung-Kil
    • Development and Reproduction
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    • v.18 no.4
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    • pp.233-240
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    • 2014
  • The early growth response protein 1 (Egr-1) is a widely reported zinc finger protein and a well known transcription factor encoded by the Egr-1 gene, which plays key roles in many aspects of vertebrate embryogenesis and in adult vertebrates. The Egr-1 expression is important in the formation of the gill vascular system in flounders, which develops during the post-hatching phase and is essential for survival during the juvenile period. However, the complete details of Egr-1 expression during embryo development in olive flounder are not available. We assessed the expression patterns of Egr-1 during the early development of olive flounders by using reverse transcription polymerase chain reaction (RT-PCR) analysis. Microscopic observations showed that gill filament formation corresponded with the Egr-1 expression. Thus, we showed that Egr-1 plays a vital role in angiogenesis in the gill filaments during embryogenesis. Further, Egr-1 expression was found to be strong at 5 days after hatching (DAH), in the development of the gill vascular system, and this strong expression level was maintained throughout all the development stages. Our findings have important implications with respect to the biological role of Egr-1 and evolution of the first respiratory blood vessels in the gills of olive flounder. Further studies are required to elucidate the Egr-1-mediated stress response and to decipher the functional role of Egr-1 in developmental stages.

A SEM STUDY OF RADIATION EFFECTS ON THE RAT MOLAR ENAMEL FORMATION (방사선조사가 백서 구치 법랑질형성에 미치는 영향에 관한 주사전자현미경적 연구)

  • Lee Kyung-Ho;Park Tae-Won
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.25 no.2
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    • pp.409-422
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    • 1995
  • The purpose of this study was to investigate the effects of radiation on the formation of rat molar enamel at the developmental stage. The experimental animals were divided into five groups and were irradiated single dose of 396cGy ; 1 st group on 14th day of gestation, 2nd group on 19th day of gestation, 3rd group on 3 days after birth, 4th group on 8 days after birth, 5th group on 28 days after birth. The control and 1, 2, 3, and 4th experimental groups were sacrificed on 2, 4, and 6 weeks and the 5th groups were sacrificed on 1 day and 2 weeks after irradiation. Distal 1/2 and occlusal 1/3 enamel surface of lingual side of lingual cusp, and fractured surface of lingual side of lingual cusp in a longitudinal direction of the mandibular first molar were examined using scanning electron microscope. The following results were obtained. 1. The roughness of enamel surface and enamel hypoplasia were increased in a sequence of 4th, 1st, 2nd, and 3rd experimental group, and the enamel cracks were increased in the 1st and 2nd experimental group. 2. The pattern of enamel hypoplasia had a network form on the 1st and 2nd experimental group, and appeared a linear shape on the 3rd experimental group, and then the crator-like enamel defects were observed in all experimental groups (especially 1st and 2nd experimental group) except 5th. 3. Dentinoenamel junction showed the clear-cut and straight appearance except 5th experimental group. 4. There was no significant difference between 5th experimental and control group.

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Induced expression of three heat shock proteins mediated by thermal stress in Heortia vitessoides (Lepidoptera: Crambidae)

  • CHENG, Jie;WANG, Chun-Yan;LYU, Zi-Hao;LIN, Tong
    • Entomological Research
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    • v.48 no.5
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    • pp.416-428
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    • 2018
  • To gain an insight into the function of heat shock proteins (HSPs) in insects during thermal stress, three HSP cDNAs were identified in the transcriptome of adult Heortia vitessoides, one of the most destructive defoliating pests in Aquilaria sinensis (Loureiro) Sprenger forests. The open reading frames of HvHsp60, HvHsp70, and HvHsp90 were 1,719, 2,070, and 2,151 bp in length, respectively, and encoded proteins with molecular weights of 61.05, 75.02, and 82.23 kDa, respectively. Sequence analysis revealed that all three HSPs were highly conserved in structure. Regarding the stage-specific expression profiles, HvHsp60, HvHsp70, and HvHsp90 mRNAs were detected in all developmental stages. Regarding the tissue-specific expression profiles, the expression levels of the three HSP genes were different in various larval and adult tissues. Moreover, the expression patterns of heat-stressed larvae, pupae, and adults indicated that HvHsp60, HvHsp70, and HvHsp90 were heat-inducible. In particular, HvHsp60 transcripts increased dramatically in larvae and pupae that were heat-stressed at $40^{\circ}C$ and were upregulated in adults that were heat-stressed at $35^{\circ}C$ and $40^{\circ}C$. The expression of HvHsp70 significantly increased in all of the three different developmental stages at $35^{\circ}C$, $40^{\circ}C$, and $45^{\circ}C$. The expression of HvHsp90 obviously increased at $30^{\circ}C$, $35^{\circ}C$, and $40^{\circ}C$ in larvae and could be induced at $35^{\circ}C$ in pupae and adults. The results suggest that HSP60, HSP70, and HSP90 play a major role in protecting H. vitessoides against high-temperature stress.

Expressional Evaluation of C/EBP Family, SREBP1, and Steroid Hormone Receptors in the Epididiymal Fat of Postnatally Developing Mouse

  • Lee, Yong-Seung;Lee, Ki-Ho
    • Development and Reproduction
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    • v.26 no.2
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    • pp.49-58
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    • 2022
  • The differentiation and development of preadipocyte into mature adipocyte are regulated by transcription factors, such as CCAAT enhancer binding protein (Cebp) gene family and sterol regulatory element binding transcription factor 1 (Srebp1). Steroid hormones give influences on the development and function of adipocyte. The present research examined expression patterns of CCAAT enhancer binding protein alpha (Cebpa), CCAAT enhancer binding protein beta (Cebpb), CCAAT enhancer binding protein gamma (Cebpg), sterol regulatory element binding transcription factor 1 (Srebp1), androgen receptor (Ar), and estrogen receptors (Esr) among different epididymal fat parts during postnatal period by quantitative real-time polymerase chain reaction. In the distal epididymal fat, expression of Cebpa, Cebpb, Cebpg, Srebp1, Ar, and Esr2 was increased until 12 months of age, while expression of Esr1 was decreased at 5 months of age and was not detectable after 8 months of age. In the proximal epididymal fat, transcript levels of Cebps and Srebp1 were increased at 8 months of age, followed by decreases of Cebpb and Cebpg transcript levels at 12 months of age. An additional increase of Srebp1 expression was observed at 12 months of age. Expression of Ar and Esr2 were increased until 8 months of age, followed by a drop of Ar expression level at 12 months of age. Expression pattern of Esr1 was similar to that in the distal epididymal fat. In the tail epididymal fat, expression of Cebpa, Cebpg, Srebp1, Ar, and Esr2 was increased with age. Esr1 was not detectable at all. The highest level of Cebpb was observed at 8 months of age. These data suggest the possibility of developmental and functional differentiation among the epididymal fat parts.