• 한국가족복지학
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• 제54호
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• pp.263-296
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• 2016

이 연구는 발달장애 자녀를 둔 어머니의 양육부담과 성장경험을 이해하고자 한 것이다. 이를 위해 오케스트라 활동에 참여하는 발달장애 자녀를 둔 어머니를 대상으로 포토보이스를 활용한 초점집단인터뷰와 개별면접을 실시하였다. 분석 결과, 발달장애 자녀를 둔 어머니의 양육 경험은 "눈앞이 캄캄해도 걸어감"으로 나타났으며, 이 과정에서 어머니들은 "동행하며 함께 나아감"을 경험하는 것으로 나타났다. 어머니들은 자녀의 발달장애로 인해 여러 고통과 어려움을 경험하면서도 양육과정에서 또 다른 기쁨과 성장을 경험하고 있었다. 특히 오케스트라 활동이라는 매개체를 통해, 어머니는 자녀에 대한 인식 뿐 아니라 양육자로서의 자신에 대한 인식, 주변 지지체계와의 관계에도 의미있는 변화가 나타남이 확인되었다. 이러한 결과를 바탕으로 이 연구는 사회복지적 함의를 제시하고 있다.

• Clinical and Experimental Pediatrics
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• 제57권8호
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• pp.363-369
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• 2014

Purpose: This study examines changes in developmental profiles of children with language delay over time and the clinical significance of assessment conducted at age 2-3 years. Methods: We retrospectively reviewed the medical records of 70 children (62 male, 8 female), who had visited the hospital because of delayed language development at 2-3 years, and were reassessed at ages 5-6. Language and cognitive abilities were assessed using multiple scales at the initial and follow-up visits. Results: At the initial test, 62 of the 70 children had mental development index (MDI) below 70 of Bayley Scales of Infant Development Test II. Of the 62 children in the follow-up assessment, 30 children (48.4%) remained within the same cognitive range (full-scale intelligence quotient, FSIQ<70 of Wechsler preschool and primary scale of intelligence), 12 had borderline intellectual functioning (FSIQ, 70-85), 6 improved to average intellectual functioning (FSIQ>85), and 5 had specific language impairment, 9 had autism spectrum disorders. At the initial test, 38 of the 70 children had cognitive developmental quotients (C-DQ) below 70. Of the 38 children in the follow-up assessment, 23 children (60.5%) remained within the same cognitive range (FSIQ<70). The correlation coefficient for MDI and FSIQ was 0.530 (P<0.0001) and that for C-DQ and FSIQ was 0.727 (P<0.0001). There was a strong correlation between C-DQ and FSIQ, and a moderate correlation between MDI and FSIQ. Conclusion: Low MDI scores reflect a specific delay in cognitive abilities, communication skills, or both. The C-DQ, receptive language development quotient, and social maturity quotient also help to distinguish between children with isolated language delay and children with cooccurring cognitive impairment. Moreover, changes in the developmental profile during preschool years are not unusual in children with language delay. Follow-up reassessments prior to the start of school are required for a more accurate diagnosis and intervention.

• 한국발생생물학회지:발생과생식
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• 제16권4호
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• pp.329-338
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• 2012

The proliferation of embryonic cells or adult stem cells in tissue is critically regulated during development and repair. How limited the proliferation of cells, so far, is not much explored. Cell-cell contact proliferation inhibition is known as a crucial mechanism regulating cell proliferation in vitro and in vivo. In this study we examined the characters of mouse subcutaneous adipose derived stem cells (msADSC) whether they lost or get contact inhibition during in vitro culture. The characters of msADSC growth after confluence were analyzed using confocal microscope and the expression profiles of contact inhibition related genes were analyzed according to the morphological changes using real-time PCR method. msADSC showed overlapping growth between them but not after passage 14. The cell shapes were also changed after passage 14. The expression profiles of genes which are involved in contact inhibition were modified in the msADSC after passage 14. The differentiation ability of msADSCs to adipocyte, chondrocyte and osteocyte was not changed by such changes of gene expression profiles. Based on these results, it is revealed that smADSC were characterized by getting of strong cell-cell contact inhibition after passage 14 but the proliferation and developmental ability were not blocked by the change of cell-cell contact proliferation inhibition. These finding will help to understand the growth of adipose tissue, although further studies are needed to evaluate the physiological meaning of the cell-cell contact proliferation inhibition during in vitro culture of msADSC.

• 한국패류학회지
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• 제17권1호
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• pp.27-33
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• 2001

The Ovarian developmental phases of the reproductive cycle of Rapana venosa can be classified into five successive stages by histological study: early active stage (September to February), late active stage (December to April), ripe stage (March to July), partially spawned stage (May to August), and recovery stage (June to September). To understand the characteristics of nutrient storage and utilization in the digestive gland cells with ovarian developmental phases, we examined the digestive gland - which is the major nutrient supply organ associated with ovarian development of the female purple shell - by biochemical methods. Total protein contents in the digestive gland tissues increased in March (late active stage) and reached the maximum in May (ripe and partially spawned stages), and then their levels sharply decreased in July (partially spawned and recovery stages). Total lipid contents in the digestive gland tissues reached the maximum in January (early active stage). Thereafter, their levels rapidly decreased from May (ripe and partially spawned stages) and reached a minimum in July (partially spawned and recovery stages). The total DNA contents did not significantly change regardless of the different developmental stages of the ovary. However, it was also found from biochemical analysis that changes in total RNA content follow the same seasonal cycling to protein. These results indicate that the digestive gland is an important energy storage and supply organ in purple shells, and that the nutrient contents of the digestive gland change in response to gonadal energy needs.

• 아동학회지
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• 제22권1호
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• pp.97-107
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• 2001

This study argues the nature of the microgenetic method in respect to child development research and explores its merits and limitations. The microgenetic method focuses on observations that span the entire period from the beginning of developmental change to the time it reaches a relatively stable state. This produces a high density of observations relative to the rate of change in the phenomenon. In this way, observed behavior may be used in intensive trial-by-trial analysis. The microgenetic method is superior to other methods in the possibilities for observing developmental changes as they occur, examining various aspects of change, detecting variability in behavior, and flexibility in application regardless of theoretical perspectives. Limitations of this method include reliability problems due to repeated observations, artificiality relative to the natural situation, inconsistency of short-and long-term change, and demands on children's motivation for participation in research.

• 한국문헌정보학회지
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• 제31권1호
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• pp.125-151
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• 1997

이 연구는 현 단계 한국문헌정보학이 각종 사회환경의 변화에 유연하게 대응할 수 있는 발전전략을 모색하는 것이다. 이를 위해 다음의 두 가지 실천전략을 제시하였다. 첫 번째는 주체적인 학술연구의 전략으로서, 연구자들이 기존의 학술논문쓰기 방법을 과감하게 탈피하여 도서관과 관련기관의 일상생활을 있는 그대로 기술하고 설명할 수 있는 자유롭고 쉬운 양식의 글 쓰기 전략을 제시하였다. 또한 한국의 낙후된 도서관현장의 발전에 참조가 되는 방향에서 외국의 원전을 읽고 번역하는 전략도 함께 제시하였다. 두 번째는 학술운동의 전략으로서, 연구자와 현장사서들이 함께 참여하여 우리의 학문적 내실을 다져내며 한국문헌정보학이 한국사회과학의 당당한 일원으로서 참여할 수 있는 전략을 제시하였다.

• Journal of Ginseng Research
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• 제43권2호
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• pp.242-251
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• 2019

Background: Korean Red Ginseng has been widely used in traditional oriental medicine for a prolonged period, and its pharmacological effects have been extensively investigated. In addition, Angelica gigas and deer antlers were also used as a tonic medicine with Korean Red Ginseng as the oriental herbal therapy. Methods: This study was conducted to evaluate the potential toxicological effect of KGC-HJ3, Korean Red Ginseng with angelica gigas and deer antlers, on reproductive and developmental functions including fertility, early embryonic development, maternal function, and embryo-fetal development. KGC-HJ3 was administered by oral gavage to Sprague-Dawley rats (22 animals per sex per group) at dose levels of 0 mg/kg (control), 500 mg/kg, 1000 mg/kg, and 2000 mg/kg to evaluate the potential toxicological effect on fertility and early embryonic development. In addition, KGC-HJ3 was also administered by oral gavage to mating-proven Sprague-Dawley rats (22 females per group) during the major organogenesis period at dose levels of 0 mg/kg (control), 500 mg/kg, 1000 mg/kg, and 2000 mg/kg to evaluate the potential toxicological effect on maternal function and embryo-fetal development. Results and conclusion: No test item-related changes in parameters for fertility, early embryonic development, maternal function, and embryo-fetal development were observed during the study period. On the basis of these results, it was concluded that KGC-HJ3 did not have toxicological potential on developmental and reproductive functions. Therefore, no observed adverse effect levels of KGC-HJ3 for fertility, early embryonic development, maternal function, and embryo-fetal development is considered to be at least 2000 mg/kg/day.

• The Korean Journal of Physiology and Pharmacology
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• 제4권3호
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• pp.169-175
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• 2000

To learn the developmental changes in intrinsic electrophysiological properties of the second order taste neurons, whole cell recordings from the developing nucleus of the solitary tract neurons were done in brainstem slices of postnatal rats. Rats aged from postnatal 0 to 21 days (P0-P21) were used, being divided into 3 age groups: postnatal first week (P0-P7 days), second week (P8-P14 days), and third week (P15-P21 days). Slices containing gustatory NTS were cut horizontally in the thickness of $300\;{\mu}m.$ Whole cell recordings were obtained from neurons in response to a series of hyperpolarizing and depolarizing current pulses. The intrinsic electrophysiological properties of the rostral NTS (rNTS) neurons were compared among the age groups. Depolarizing current pulses evoked a train of action potentials in all neurons of all age groups. The resting membrane potential and input resistance of the neurons did not show any significant differences during the postnatal 3 weeks. The time constant, however, decreased during the development. Duration of action potential measured at half maximum amplitude was longer in younger age groups. Both the maximum rate of rise and the maximum rate of fall in the action potential increased during the first 3 weeks postnatal. Electrophysiologically more than half neurons were type III. In summary, it is suggested that developmental changes in electrophysiological properties in rNTS occur during the first three weeks in rats.

• 한국가금학회지
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• 제21권3호
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• pp.175-182
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• 1994

An experiment was conducted to establish a large scale production method of anti-serum against chicken IgM and to profile the developmental changes of serum IgM levels during the feeding period(from hatching to 7 weeks of age) in broiler chicks. Blood samples were taken from Hubbard chicken at the age of hatching, three days of age, and weekly thereafter till to 7 weeks of age. The pure IgM was isolated from ammonium sulfate treated chicken serum by both sephadex G-200 and sepharose CL-6B chromatography. The breaking-through peak containing IgM appeared from the fraction 26 to 28. These fractions consisted mainly of IgM when tested by anti-chicken IgM(Nordic, Netherlands). Immunized with the heavy chain of this purified IgM, the rabbit immune sera(anti-chicken IgM) were formed a reaction only with the purified chicken IgM. The quantitative assay of serum IgM were carried by RID method. The optimal time for diffusion was 14 hours and the coefficient of determination($R^{2}$) for regression equation of standard curve was 0.992. It was observed that IgM concentrations were the highest at hatching(3.23 mg /mL), after that decreased gradually. From 2 to 5 weeks of age the levels unchanged(2.0 ~ 2.3mg /mL), and gradually decreased to 7 weeks of age(1.3 mg /mL).

• Applied Microscopy
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• 제44권4호
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• pp.111-116
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• 2014

This study investigates the developmental pattern of adhesive discs (ADs) to highlight the ontogeny and structural changes that occur during the growth of Boston ivy. Initiation to postmortem features of ADs were examined through light and scanning electron microscopy. The study also reveals a new finding of the dislocation of peripheral tissues of adaxial origin. Four phases of attachment are suggested with regards to its climbing behavior: 1) pre-attachment, 2) upon attachment, 3) after attachment, and 4) final attachment. During initiation, several ADs originate from tendril primordia without epidermal differentiation. However, different growth rates in the epidermis results in completely different ADs. ADs were discerned by size, shape, and color during expansion, but cells in the adaxial surface remained alive longer than the other side. Upon contact, the ADs demonstrate simultaneous growth and deterioration, but once attachment is established the latter process subdues to final stages. Epidermal transformation, adhesive secretion, cellular disruption, and mechanical stress were essential for the self-clinging nature of Boston ivy. The post-attachment sequence is also believed to be critical in achieving maximum mechanical strength to provide extensive support. The developmental process of ADs is prompted by tactile stimulation but in a highly organized and systematic manner.