• 한국환경과학회지
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• 제7권6호
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• pp.811-815
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• 1998

Currently in Korea, standard operating procedure for the analyses of phenolic compounds in water is the spectrometric comparison of colors developed by 4-amino antipyrin with phenolic compounds. It is however that this method cannot identify individual compound and that some phenolic compounds do not react with 4-amino antipyrin. Spectrometric determinations of phenolic compounds were compared with chromatographic analyses of gas chromatography (GC) and high pressure liquid chromatography (HPLC) of various phenolic compounds. Individual phenolic compounds could be determined by both chromatographic methods but HPLC methods were more precise with lower detection levels in general.

• 한국대기환경학회지
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• 제18권2호
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• pp.127-137
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• 2002

Phenolic compounds in air are toxic even at their low concentrations. We had evaluated a total of five phenolic compounds (Phenol, o-Cresol, m-Cresol, 2-Nitrophenol and 4-Chloro-3-methylphenol) in artificial air using a combination of ATD/GC/MS. To compare the adsorption efficiency of these phenolic compounds, three adsorbents (Tenax TA, Carbotrap and Carbopack B) were tested. Tenax TA adsorbent was most effective of all the adsorbents used for the efficiency test. Five phenolic compounds were found to be very stable on adsorbent tubes for 4 days at room temperature. Detection limit of five phenolic compounds ranged from 0.05 to 0.08 ppb (when assumed to collect 10 L air). The calibration curve was linear over the range of 22∼ 164 ng. The reproducibility was less than 4%. Sampling of duplicate pairs (DPs) was made to demonstrate duplicate precision and sampling efficiency.

• Bulletin of the Korean Chemical Society
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• 제31권12호
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• pp.3755-3759
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• 2010

A on-line SPE (solid phase extraction)-HPLC preconcentration method was developed for the determination of phenolic compounds at trace levels in environmental water sample. XAD-4 and Dowex 1-X8 were used as sorbent in the on-line SPE-HPLC method for the selective enrichment of nine phenolic compounds, which are included in the priority pollutants list of the US EPA. Also alumina prefiltering considerably reduced the amount of interfering peaks due to humic substances that could accumulated due to the preconcentration step and prevent quantification of polar phenolic compounds in environmental water samples. This method was used to determine the phenolic compounds in tap and river water and superiority to the US EPA 625 method in its enrichment factor, pretreatment time, recoveries, and detection limit. The limits of detection were in the range of $0.3-0.9\;{\mu}g/L$ in tap water sample.

• Preventive Nutrition and Food Science
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• 제3권2호
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• pp.111-118
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• 1998

To develop a simple, rapid and simultaneous analytical method of phenolic compounds using gas chromatography (GC) and gas chromatography/mass spectrophometer (GC/Ms), this experiment was carried out to search the retention times of capillary columns and the characteristics of fragment ions in electron impact mass spectra. Most of trimethylsilyl derivatives and underivatized phenolic compounds were separated very well on three kinds of capillary columns(HP-1), Ultra-2 and HP-35). Quantitiative determination of phenolic compounds was achieved by internal standards (p-hydroxybenzoic acid iopropyl ester, p-hydroxybenzoic acid ethyl ester). Calibration plts were linear in the investigated range, and the limits of detection were about 5 ng at split mode method. When analyzed by three columns, theseparation times were fairly constant on two nonpolar columns, but a few compounds showed slightly different separation order by the itnermediate polar HP-35 column. The important characteristic patterns of TMS derivatives of phenolic compounds on the EI/MS spectrra appeared at the base peak of [M-15]+ ion and presented at high abundance in most TMS derivatives of phenoloc compounds. [M]+, [M-CH3-COO]+, [M-Si(CH3)4]+ and [M-Si(CH3)4 -CH3]+ also observed in mass spectra of these compounds . Although several compounds have the same retention times on GC column, it might be possible to identify these compounds by the different patternsof mass frgement ions. The TMS derivatives, thus , provide additional information for identification of phenolic compounds in biological systems.

• 폴리머
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• 제36권4호
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• pp.470-477
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• 2012

다양한 아민 그룹으로 개질된 다중벽 탄소나노튜브(이하 MWNT) 지지체를 기반으로 하여 페놀 화합물을 검출하기 위한 티로시나아제가 고정된 바이오센서를 개발하였다. 방사선 중합법을 이용하여 MWNT에 글리시딜메타크릴레이트를 중합한 후 중합 사슬의 아미노화 반응을 통해 MWNT에 다양한 아민 그룹을 도입시켰다. 이렇게 제조된 물질의 물리적, 화학적 특성은 SEM, XPS 그리고 TGA에 의해 평가되었다. 그리고 제조된 물질을 기반으로 제작된 티로시나아제가 고정화된 바이오센서의 전기화학적 특성도 평가하였다. 본 효소 바이오센서는 0.1-0.9 mM의 페놀을 검출할 수 있다. 결합효과, pH, 온도 그리고 다양한 페놀화합물에 대한 반응과 같은 여러 가지 변수에 대하여도 최적화하였고 상용 레드와인에서의 페놀화합물 검출도 연구하였다.

• 생명과학회지
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• 제21권9호
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• pp.1323-1328
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• 2011

본 연구에서는 페놀 화합물들을 현장에서 검출하기 위해 간단하고 간편한 일회용 재조합 박테리아 바이오센서 시스템을 개발하였다. 플라즈미드 pLZCapR을 함유하는 E. coli 세포를 ${\beta}$-galactosidase 기질인 CPRG와 함께 96-well plate의 wells에 agarose로 고정하였다. 이 바이오센서는 현장에서 발색을 위한 별도의 기질을 추가하거나 불편한 기기 사용 또는 시료의 전 처리를 필요로 하지 않는다. 시료의 측정은 간단히 적은 부피(<100 ${\mu}l$)의 현장 시료를 바이오센서를 포함하는 wells에 넣고 발색을 관찰하여 측정하였다. 또한 6% DMF, 0.1% SDS 그리고 10 mM $CaCl_2$를 첨가하여 agarose 고정에 의한 화합물의 세포내 확산 제한을 감소시켜 보다 더 나은 발색을 얻을 수 있었다. 따라서 이 고정된 미생물 유래 재조합 바이오센서 시스템은 현장에서 환경오염물질들을 간단하게 확인하고 정량 하는 유용한 접근 방법이 될 것으로 사료된다.

• 한국전기화학회:학술대회논문집
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• 한국전기화학회 2005년도 춘계총회 및 학술발표회
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• pp.32-32
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• 2005

• Journal of Ginseng Research
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• 제14권3호
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• pp.437-441
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• 1990

The investigation of UV absorbing compounds in saponin fraction of Pnm.1. Kiairnk root was carried out by thin layer chromatography, semipreparative HVLC. l3C, 1H-NMR, mass spectrometry and chemical characteristics in searching plant for growth regulatony substances such as phenolic glycoside. Drying of fresh ginseng at 15 $^{\circ}C$ decreased not only number but also size of UV absorbing sports on TLC. One of the relatively large spots in fresh ginseng was isolated and identified as adenosine, which is subjected for growth stimulatory activity Detection of phenolic glycosides failed in dried root bolt was highly probable in fresh ginseng even with the insufficient amount of sample.

• KSBB Journal
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• 제29권1호
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• pp.1-8
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• 2014

Tyrosinases catalyze the hydroxylation of monophenolic compounds and the conversion of o-diphenols to oquinones. The enzymes are mainly involved in the modification of tyrosine into L-3,4-dihydroxyphenyl-alanine (L-DOPA) and DOPA/DOPAquinone-drived intermolecular cross-linking, which play the key roles of pigmentation to the cells. It is ubiquitously distributed in microorganisms, plants, and animals all around the nature world. They are classified as copper- containing dioxygen activating enzymes; two copper ions are coordinated with six histidine residues in their active sites and they are distinguished as met-, deoxy-, and oxy-form depending on their oxidative states. Natural extraction and recombinant protein approaches have been tried to obtain practical amounts of the enzymes for industrial application. Tyrosinases have been widely applied to industrial and biomedical usages such as detoxification of waste water containing phenolic compounds, L-DOPA as a drug of Parkinson's disease, biomaterials preparation based on the cross-linking ability and biosensors for the detection of phenolic compounds. Therefore, this review reports the mechanism of tyrosinase, biochemical and structural features and potential applications in industrial field.

• Bulletin of the Korean Chemical Society
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• 제23권3호
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• pp.427-431
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• 2002

A screen printed graphite electrode has been developed for a simple and sensitive determination of phenolic compounds in an aqueous solution. The electrode developed uses a simple and effective screen printing technique with ${\alpha}-Cyclodextrin({\alpha}-CD)$ modified graphite ink. Phenols were captured on the surface of the ${\alpha}-CD$ modified electrode through complex formation. The phenol/ ${\alpha}-CD$ complex was deposited and quantified electrochemically using cyclic voltammetry (CV), differential pulse voltammetry (DPV) and square wave voltammetry (SWV). The optimization of the experimental parameters was performed in regard to electrode composition, pH, temperature, sample preconcentration time. Interferences from other organic compounds were investigated. The detection limit for phenols was 500 ${\pm}7$ nM for DPV, with the linear range of 0.5 ${\mu}M$ -25.0 ${\mu}M$ and 30 ${\pm}2$ nM for SWV, with the linear range of 30 nM - $50{\mu}M$, respectively.