• Journal of Microbiology and Biotechnology
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• 제21권8호
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• pp.830-837
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• 2011

A genomic DNA fragment encoding a putative maltooligosyltrehalose trehalohydrolase (NfMTH) for trehalose biosynthesis was cloned by the degenerate primer- PCR from cyanobacterium Nostoc flagelliforme. The ORF of NfMTH is 1,848 bp in length and encodes 615 amino acid residues, constituting a 70 kDa protein. The deduced amino acid sequence of NfMTH contains 4 regions highly conserved for MTHs. By expression of NfMTH in E. coli, the function of this protein was demonstrated, where the recombinant protein catalyzed the hydrolysis of maltooligosyl trehalose to trehalose. The expressions of MTH and maltooligosyltrehalose synthase in the filaments of N. flagelliforme were upregulated significantly under dehydration stress, NaCl stress, and high temperature-drought stress. The accumulations of both trehalose and sucrose in the filaments of N. flagelliforme were also improved significantly under the above stresses. Furthermore, trehalose accumulated in smaller quantities than sucrose did when under NaCl stress, but accumulated in higher quantities than sucrose did when under temperature-drought stress, indicating that both trehalose and sucrose were involved in N. flagelliforme adapted to stresses and different strategies conducted in response to various stress conditions.

• 한국지반환경공학회 논문집
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• 제3권1호
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• pp.67-77
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• 2002

준설토의 체적변화는 여수토를 통해 물과 함께 빠져나간 토립자의 유실량과 침강되어 새로이 형성된 지반 표면에서의 건조수축과 지반 내에서의 자중압밀에 의한 침하량의 합으로 생각할 수 있다. 그러나 현재까지 체적변화요인과 관련하여 자중압밀과 건조수축에 관한 연구에 비해 토립자의 유실과 관련된 연구는 미미한 실정이다. 따라서 본 연구에서는 준설토의 체적변화요인 중 여수토를 통해 외부로 유실되는 토량을 보다 명확히 규명하기 위해, 준설작업을 실시하기 전의 원지반과 준설토를 투기하여 조성된 매립 지역에서 각각 채취한 시료에 대한 입도분석결과에 Marsal의 수정파쇄율(일본토질공학회, 1990)을 적용하여 유실율을 평가하는 방법을 적용하였고, 이를 검증하기 위하여 기존의 방법을 이용하여 평가한 유실율과 비교 검토해 보았다. 또한 실내모형시험을 실시하여 현장계측 결과와 비교 검토하였으며 마지막으로 준설토를 단계투기법에 의하여 투기했을때 유실율에 미치는 영향을 실내모형시험을 통하여 확인하였다.

• Parasites, Hosts and Diseases
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• 제55권3호
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• pp.233-238
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• 2017

Pathogenic Naegleria fowleri, Acanthamoeba castellanii, and Acanthamoeba polyphaga, are distributed worldwide. They are causative agents of primary amoebic meningoencephalitis or acanthamoebic keratitis in humans, respectively. Trophozoites encyst in unfavorable environments, such as exhausted food supply and desiccation. Until recently, the method of N. fowleri encystation used solid non-nutrient agar medium supplemented with heat-inactivated Escherichia coli; however, for the amoebic encystment of Acanthamoeba spp., a defined, slightly modified liquid media is used. In this study, in order to generate pure N. fowleri cysts, a liquid encystment medium (buffer 1) modified from Page's amoeba saline was applied for encystation of N. fowleri. N. fowleri cysts were well induced after 24 hr with the above defined liquid encystment medium (buffer 1). This was confirmed by observation of a high expression of differential mRNA of nfa1 and actin genes in trophozoites. Thus, this liquid medium can replace the earlier non-nutrient agar medium for obtaining pure N. fowleri cysts. In addition, for cyst formation of Acanthamoeba spp., buffer 2 (adjusted to pH 9.0) was the more efficient medium. To summarize, these liquid encystment media may be useful for further studies which require axenic and pure amoebic cysts.

• 식물조직배양학회지
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• 제27권5호
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• pp.415-421
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• 2000

The disaccharide trehalose ($\alpha$-D-glucopyranosyl-$\alpha$-D-glucopyranoside) is found in variety of organ-isms that are able to withstand almost complete desiccation. In order to identify the function of trehalose in plants, we isolated Arabidopsis trehalase (AtTRE) gene that encodes the enzyme able to hydrolyze trehalose to glucose, and trehalose-6-phosphate synthase isolog, TPS3 gene by RT-PCR. The AtTRE had the substrate specificity to hydrolyze only trehalose, and a broad pH range of enzyme activity. The AtTRE promoter/GUS reporter gene was expressed in cotyledons, mature leaf tissues including guard cells, and developing siliques. The GUS expression driven by AtTPS3 promoter was significant in root tissues, and the level of GUS activity was much higher than that of the pBll 21 control seedlings. The knockout of AtTPS3 gene in Arabidopsis resulted in the retarded root development, whereas the overexpression of AtTPS3 increased the root elongation in the presence of sucrose in MS medium. Possible functions of AtTRE and AtTPS3 in plant will be discussed. In addition, ectopic expression of yeast TPS1 driven by the inducible promoters in tobacco and potato conferred the plants on the drought and freezing tolerances.

• 한국환경복원기술학회지
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• 제5권2호
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• pp.34-38
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• 2002

자생 양치식물중 실내식물로서 개발가치가 높은 가는쇠고사리(Arachniodes aristata )와 석위(Pyrrosia lingua)의 실내 습도조건에 따른 생육 반응을 살펴보고자 수행하였으며, 그 결과는 다음과 같다. 1. 가는고사리와 석위는 상대습도 50%, 90% 보다 25%와 같은 저습도시 식물체내수분함량, 엽록소함량, 관상가치가 저하되었다. 2. 상대습도에 대한 용토별 생육에 있어 고습도인 90%일시 용토별 생육에는 큰 차이가 나타나지 않았으나, 중습도인 50%일 경우 일반 토양이 가장 좋은 것으로 나타났다. 저습도인 25%의 경우 가는쇠고사리는 인공배합토에서, 석위는 일반토양에서 생육이 좋은 것으로 나타났다.

• 한국지반공학회논문집
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• 제19권4호
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• pp.55-64
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• 2003

흙의 건조현상은 기상조건에 지배받는 evaporativity와 지반의 물 전달 능력에 지배받는 evaporability의 영향을 받는다. 지표면증발은 점토로 매립된 지반에서 건설장비의 주행성을 확보하기 위해서는 필요불가결한 요소이다. 본 논문에서는 준설 점토 지반에서의 증발현상을 규명할 수 있는 모델을 제시하였다. 이 모델은 포화토 및 불포화토에서의 수분과 물의 이동 및 열전달에 대한 식으로 구성되어 있다. 흙수분 특성곡선을 구하기 위하여 압력판추출시험(pressure plate extractor test)과 데시케이터실험이 수행되었으며 일차원 증발현상을 규명하기 위하여 컬럼증발실험을 수행하였다. 체적이 변하는 흙에서의 물, 증기 및 열전달 비선형 미분방정식을 풀기 위하여 유한차분프로그램을 개발하였으며 실험결과와 이론해를 비교하였다.

• Geomechanics and Engineering
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• 제14권3호
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• pp.283-291
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• 2018

Cracks in soil provide significant preferential pathways for contaminant transport and rainfall infiltration. Water exchange between the soil matrix and crack is crucial to characterize the preferential flow, which is often quantitatively described by a water exchange ratio. The water exchange ratio is defined as the amount of water flowing from the crack into the clay matrix per unit time. Most of the previous studies on the water exchange ratio mainly focused on cracked sandy soils. The water exchange between cracks and clay matrix were rarely studied mainly due to two reasons: (1) Cracks open upon drying and close upon wetting. The deformable cracks lead to a dynamic change in the water exchange ratio. (2) The aperture of desiccation crack in clay is narrow (generally 0.5 mm to 5 mm) which is difficult to model in experiments. This study will investigate the water exchange between a deformable crack and the clay matrix using a newly developed experimental apparatus. An artificial crack with small aperture was first fabricated in clay without disturbing the clay matrix. Water content sensors and suction sensors were instrumented at different places of the cracked clay to monitor the water content and suction changes. Results showed that the water exchange ratio was relatively large at the initial stage and decreased with the increasing water content in clay matrix. The water exchange ratio increased with increasing crack apertures and approached the largest value when the clay was compacted at the water content to the optimal water content. The effective hydraulic conductivity of the crack-clay matrix interface was about one order of magnitude larger than that of saturated soil matrix.

• ALGAE
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• 제17권1호
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• pp.41-51
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• 2002

Three dominant rocky intertidal macroalgae, the fucoids Fucus gardneri and Pelvetiopsis limitata (Phaeophyta) and the red alga Mazzaella cornucopiae (= Iridaea cornucopiae) on the west coast of Vancouver Island, British Columbia, Canada were used in a series of field experiments to examine interspecific interactions. These experiments showed complex patterns which included an interchange of negative (inhibition) and positive (facilitation) interactions depending on neighbor distance. Less fucoid recruitment occurred in the plots with greater percent cover of a turfforming red alga, M. cornucopiae. However, experimentally removing Mazzaella turf (the turf was considered to be "blocking" fucoid recruits or "shading" growing recruits) did not increase recruitment. This result indicated that there may be another factor(s) involved in the survivorship of juvenile fucoids in the turf-removed plots. Morphological differences in adult plants between Mazzaella and the two fucoids resulted in another type of interaction; these began when fucoids successfully settled and grew nearby or within the red algal turf. By monitoring microhabitat at the individual plant level for two years, I found that survivorship of fucoid recruits showed different species-specific patterns. The patterns also varied as the microhabitat changed from Mazzaella turf edge to open space. For F. gardneri, longevity of P.limitata at all distances tested was similar. A reason for greater longevity of F. gardneri individuals at edge microhabitats may be that these sites have one side open to light and nutrients and another site that buffers them from desiccation and wave impact. In the Mazzaella-Fucus interaction, neighbor distance was a key factor in determining whether the outcome of the interaction would be competition or facilitation (or protection). This study provides experimental evidence that detectable biological interactions occur in this upper intertidal algal community where physical conditions are usually severe, and also indicates the importance of small scale examination in understanding macroalgal interactions in intertidal habitats.

• 대한한의학방제학회지
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• 제14권1호
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• pp.74-81
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• 2006

Objectives: The aim of this work is the making of morphological standard in platycodi radix. Methods: The external-internal morphological standards were determined by using stereoscope and butanol series. Conclusions: The external and internal characteristics according to the shape of original plant or herbal states. entirely correspond to all the literature on this subject. According to the place of production. the difference appears. (1) In the external shapes. as the Korean products. the distinction among the first. medium. poor class products was caused by the state of processing of desiccation. But as the foreign products, it was caused by the difference of size, color and especially color of circle. (2) In the internal shapes. the Mt. Baekdu region products is not skinned and has the deep state of suberization of cork phellogen. so it makes certain distinctional point. And the Korean products has the much latex tube group relatively and the bigger crevice than the foreign one.

• Ocean and Polar Research
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• 제36권2호
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• pp.145-156
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• 2014

Macrobenthic biodiversity in the rocky intertidal areas of the Tae-an region, Republic of Korea, has decreased since the Hebei Spirit oil spill in December 2007. We aimed to investigate ecological roles of Pacific oyster (Crassostrea gigas) because recruitment and growth of oysters are critical to the recovery of damaged rocky shore ecosystem. We surveyed two sites monthly: natural rocky substrate and farming substrate, from July 2012 to January 2013 to identify and compare the changes in macrobenthic fauna. The abundance of young oysters was higher at the natural site. On the other hand, the mean height of oyster on the farming substrate was more than twice as great. The abundance of oyster at the natural site increased until October and then continuously decreased until end of study period. However, the abundance of oyster at the farming site constantly decreased from the beginning of study period. These different growth patterns might be attributable to spatial competition between oyster and a barnacle species (Balanus albicostatus) and environmental factors. At the natural site, physical stress factors including dramatic temperature changes and desiccation a few of the major factors limiting growth during aerial exposure. In addition, motile macrobenthos could be detrimental to oysters because they interrupt filter-feeding activities and hence hamper the growth of oysters. We show the higher recruitment of oysters at the natural site and healthy growth in the farming substrate are due to complicated differences in physical and biological stress factors.