• 한국심리학회지 : 문화 및 사회문제
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• 제26권3호
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• pp.283-301
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• 2020

한국사회에서는 인터넷 커뮤니티와 게시글 댓글 상에서 여성혐오 단어가 생성되고 확산되고 있다. 본 연구는 여성혐오 단어에 대한 노출 정도가 개인이 가지는 여성혐오 태도에 미치는 영향을 알아보기 위해 실시되었다. 연구 1에서는 설문을 통해 여성혐오 단어의 노출 정도(알고 있는 여성혐오 단어 수, 인터넷 댓글을 보는 정도)와 명시적 여성혐오 태도 간의 관계를 알아보았다. 그 결과 혐오단어 노출이 많을수록 명시적 여성혐오 태도는 감소하였는데 이는 남성에게서 더 두드러지게 나타났다. 이는 부정적 미디어 자극에 대한 반복적 노출로 인한 자극 둔감화로 설명될 수 있다. 연구 2에서는 실험을 통해 여성혐오 단어의 노출 여부를 조작하고 암묵적 연합검사를 통해 암묵적 여성혐오 태도 간의 관계를 알아보았다. 집단 간 분산 분석 결과 남성의 경우 여성에 비해 혐오단어에 노출될수록 암묵적 여성혐오 태도가 더 강한 것으로 나타났다. 본 연구의 결과는 여성혐오 태도에 있어 명시적 태도와 암묵적 태도 간의 차이를 보여주며, 혐오단어에 대한 동일한 수준의 노출 정도가 남성과 여성의 태도 변화에 미치는 영향이 다를 수 있음을 시사한다.

• The Korean Journal of Physiology and Pharmacology
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• 제27권4호
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• pp.417-426
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• 2023

The TRPM4 gene encodes a Ca²⁺-activated monovalent cation channel called transient receptor potential melastatin 4 (TRPM4) that is expressed in various tissues. Dysregulation or abnormal expression of TRPM4 has been linked to a range of diseases. We introduced the hemagglutinin (HA) tag into the extracellular S6 loop of TRPM4, resulting in an HA-tagged version called TRPM4-HA. This TRPM4-HA was developed to investigate the purification, localization, and function of TRPM4 in different physiological and pathological conditions. TRPM4-HA was successfully expressed in the intact cell membrane and exhibited similar electrophysiological properties, such as the current-voltage relationship, rapid desensitization, and current size, compared to the wild-type TRPM4. The presence of the TRPM4 inhibitor 9-phenanthrol did not affect these properties. Furthermore, a wound-healing assay showed that TRPM4-HA induced cell proliferation and migration, similar to the native TRPM4. Co-expression of protein tyrosine phosphatase, non-receptor type 6 (PTPN6 or SHP1) with TRPM4-HA led to the translocation of TRPM4-HA to the cytosol. To investigate the interaction between PTPN6 and tyrosine residues of TRPM4 in enhancing channel activity, we generated four mutants in which tyrosine (Y) residues were substituted with phenylalanine (F) at the N-terminus of TRPM4. The YF mutants displayed properties and functions similar to TRPM4-HA, except for the Y256F mutant, which showed resistance to 9-phenanthrol, suggesting that Y256 may be involved in the binding site for 9-phenanthrol. Overall, the creation of HA-tagged TRPM4 provides researchers with a valuable tool to study the role of TRPM4 in different conditions and its potential interactions with other proteins, such as PTPN6.

• Korean Circulation Journal
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• 제54권6호
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• pp.325-335
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• 2024

Background and Objectives: The number of sensitized heart failure patients on waiting lists for heart transplantation (HTx) is increasing. Using the Korean Organ Transplantation Registry (KOTRY), a nationwide multicenter database, we investigated the prevalence and clinical impact of calculated panel-reactive antibody (cPRA) in patients undergoing HTx. Methods: We retrospectively reviewed 813 patients who underwent HTx between 2014 and 2021. Patients were grouped according to peak PRA level as group A: patients with cPRA ≤10% (n= 492); group B: patients with cPRA >10%, <50% (n=160); group C patients with cPRA ≥50% (n=161). Post-HTx outcomes were freedom from antibody-mediated rejection (AMR), acute cellular rejection, coronary allograft vasculopathy, and all-cause mortality. Results: The median follow-up duration was 44 (19-72) months. Female sex, re-transplantation, and pre-HTx renal replacement therapy were independently associated with an increased risk of sensitization (cPRA ≥50%). Group C patients were more likely to have longer hospital stays and to use anti-thymocyte globulin as an induction agent compared to groups A and B. Significantly more patients in group C had positive flow cytometric crossmatch and had a higher incidence of preformed donor-specific antibody (DSA) compared to groups A and B. During follow-up, group C had a significantly higher rate of AMR, but the overall survival rate was comparable to that of groups A and B. In a subgroup analysis of group C, post-transplant survival was comparable despite higher preformed DSA in a desensitized group compared to the non-desensitized group. Conclusions: Patients with cPRA ≥50% had significantly higher incidence of preformed DSA and lower freedom from AMR, but post-HTx survival rates were similar to those with cPRA <50%. Our findings suggest that sensitized patients can attain comparable post-transplant survival to non-sensitized patients when treated with optimal desensitization treatment and therapeutic intervention.