• Journal of Microbiology and Biotechnology
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• 제25권5호
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• pp.579-588
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• 2015

Fungi of the genus Pestalotiopsis have drawn attention for their capability to produce an array of bioactive secondary metabolites that have potential for drug development. Here, we report the determination of a polyketide derivative compound, pestalotiollide B, in the culture of the saprophytic fungus Pestalotiopsis microspora NK17. Structural information acquired by analyses with a set of spectroscopic and chromatographic techniques suggests that pestalotiollide B has the same skeleton as the penicillide derivatives, dibenzodioxocinones, which are inhibitors of cholesterol ester transfer protein (CETP), and as purpactins A and C', inhibitors of acyl-CoA:cholesterol acyltransferase (ACAT). Strain NK17 can make a fairly high yield of pestalotiollide B (i.e., up to 7.22 mg/l) in a constitutive manner in liquid culture. Moreover, we found that a putative histone deacetylase gene, designated as hid1, played a role in the biosynthesis of pestalotiollide B. In the hid1 null mutant, the yield of pestalotiollide B increased approximately 2-fold to 15.90 mg/l. In contrast, deletion of gene hid1 led to a dramatic decrease of conidia production of the fungus. These results suggest that hid1 is a modulator, concerting secondary metabolism and development such as conidiation in P. microspora. Our work may help with the investigation into the biosynthesis of pestalotiollide B and the development for new CETP and ACAT inhibitors.

• Journal of Information Technology Applications and Management
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• 제27권1호
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• pp.187-198
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• 2020

With the development of technology and the creation of an entertainment environment for leisure, various marketing strategies are being used in the film industry. Among them, the use of the credit cookie of ending credits was very effective in producing the series. The ending credit is the time it takes to show the names of the people who made the movie, which is meaningless to the audience. There is a cost to produce a ending credit but It wasn't made because no revenue was generated. The credit cookie was inserted into this ending credit area, which brought new pleasure to the audience. Most of them were epilogue images showing the story behind the movie, NG images showing the NG situation during film production, and In videos mentioned in the movie but not shown in the movie itself. As various ideas about credit cookie were connected with marketing, a series movie and a spin-off foretelling the derivative works after the screening work were produced and have a new meaning. As a result, the time of ending credits, which had no commercial value, became the methodology of the most powerful promotional strategy. Looking at the difference between live-action film and animation in producing such credit cookie, unlike live-action films that edit the remaining parts after shooting, the NG video of the animation has a lot of time and money to produce. So, it hasn't try very well, and it seems to have been actively produced when moving from 2D animation to 3D animation. This is because 3D animation, which has already been modeled, can create new NG scenes by simply adding animating based on the layout of the created scene. Since it is possible to produce an episode movie at a low cost and time, and to use the scenes of the movie after the production, it will be necessary to strategically produce credit cookie for promotion in animation.

• 대한화학회지
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• 제47권4호
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• pp.339-344
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• 2003

Paraquat는 가장 효과적이고 널리 사용되는 제초제이지만 인간에게 매우 유독한 물질이다. 중독환자에 있어 뇨중 농도는 가장 중요한 인자중의 하나로 알려져 있으나 일반 검사실에서 이를 위한 정량검사는 거의 시행되지 않고 있다. 본 연구에서는 sodium dithionite를 이용하여 뇨중의 paraquat의 농도를 측정하는 새로운 방법의 진단 시험지를 개발하였다. 이 이차유도성 방식을 사용하여 paraquat의 농도를 측정한 결과 $0.5{\%}$ borate 완충용액(pH 8.0), 유도체 0.25 M $Na_2S_2O_4,\0.1~0.8{\%}$ PolyVinyl Pyrrolidone(PVP) 안정제, $1{\%}$ decanol의 구성비로 만들어진 진단시험지의 반응 색상이 안정하며 표준 비색표에 의한 육안적인 감별은 물론 화학 자동분석기에서의 응용성이 좋았다.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1156-1156
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• 2001

This work aimed to prove the feasibility of NIR spectroscopy to detect vegetable protein isolates (soy, pea and wheat) in milk powder. Two hundred and thirty-nine samples of genuine and adulterated milk powder (NIZO, Ede, NL) were analysed by NIRS using an InfraAlyzer 500 (Bran+Luebbe). NIR spectra were collected at room temperature, and data were processed by using Sesame Software (Bran+Luebbe). Separated calibrations for each non-milk protein added, in the range of 0-5%, were calculated. NIR data were processed by using Sesame Software (Bran+Luebbe). Prediction and validation were made by using a set of samples not included into the calibration set. The best calibrations were obtained by the PLSR. The type of data pre-treatment (normalisation, 1$\^$st/ derivative, etc..) was chosen to optimize the calibration parameters. NIRS technique was able to predict with good accuracy the percentage of each vegetable protein added to milk powder (soy: R$^2$ 0.994, SEE 0.193, SEcv 0.301, RMSEPall 0.148; pea: R$^2$ 0.997, SEE 0.1498, SEcv 0.207, RMSEPall 0.148, wheat: R$^2$ 0.997, SEE 0.1418, SEcv 0.335, RMSEPall 0.149). Prediction results were compared to those obtained using other two techniques: capillary electrophoresis and competitive ELISA. On the basis of the known true values of non-vegetable protein contents, the NIRS was able to determine more accurately than the other two techniques the percentage of adulteration in the analysed samples.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1519-1519
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• 2001

The aim of this work was to test the feasibility of NIRS in analysing textural characteristics of “Pasta Filata” cheese during the shelf-life. For this purpose, 128 samples of “Pasta Filata” cheese, subdivided into two sets on the basis of the wax used to avoid mechanical damages (paraffin, biodegradable wax), were analysed by using an InfraAlyzer 500 (Bran+Luebbe). Analyses were performed at room temperature. Samples were cut into small cylinders (D=3.2 cm, height = 1 cm), in agreement with literature information. Data were processed by using Sesame Software (Bran+Luebbe). Samples were analysed, during the shelf-life, at 90 and 120 days. In parallel, textural characteristics were detected carrying out a compression method by using an Universal Testing Machine Instron model 4301 (Instron Corporation, Canton, Massachusetts). As compression probe was used a cylinder (D = 5.8 cm, height = 3.7 cm) and a speed rate of 20mm/min was applied. The load at 20 mm of compression was recorded on sample cylinders of 1.7 cm (D) by 2 cm (height). Qualitative analysis of full spectra showed the possibility to gather samples on the basis of the days of shelf-life. The textural characteristics of cheese during the shelf-life was evaluated by comparing NIRS data with rheological results. The best correlation was obtained applying MLR to the first derivative of normalized absorbance values at seven wavelengths. Load values were plotted against the NIR prediction values based on first derivatives. NIRS proved to be an useful tool in classifying samples on the basis of the shelf-life period as well as in predicting their textural characteristics ($R^2$= 0.916, SEC = 0.192, SEP = 0.248, SEV = 0.345).

• 한국소음진동공학회논문집
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• 제27권1호
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• pp.28-35
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• 2017

최근 기존의 유압 차량 리프트는 높이제어의 어려움으로 인해 기술개발의 한계에 직면하였다. 휠 얼라이먼트나 차량의 하중 분포에 따른 미세한 불규칙적인 변형을 보상하기 위해서는 매우 정밀한 위치 제어성이 요구되고 있다. 이 연구에서는 이러한 기존 리프트 시스템의 한계를 해결하고자 매우 정교한 압력강하를 이끌어낼 수 있는 MR 밸브 시스템을 활용하여 새로운 차량 리프트를 제안하고 이에 대한 분석을 진행한다. 우선적으로 MR 밸브의 요구되는 성능을 파악하기 위해 유압 리프트의 운동방정식을 설립하고, 요구되는 압력강하를 얻기 위해 MR 밸브를 설계한다. 또한 정밀한 위치 제어 성능을 얻기 위해 PID 제어기를 설립하고, 시뮬레이션을 통해 제안된 시스템의 제어성을 검증한다.

• Steel and Composite Structures
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• 제33권6호
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• pp.891-906
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• 2019

This study considers the instability behavior of sandwich plates considering magnetorheological (MR) fluid core and piezoelectric reinforced facesheets. As facesheets at the top and bottom of structure have piezoelectric properties they are subjected to 3D electric field therefore they can be used as actuator and sensor, respectively and in order to control the vibration responses and loss factor of the structure a proportional-derivative (PD) controller is applied. Furthermore, Halpin-Tsai model is used to determine the material properties of facesheets which are reinforced by graphene platelets (GPLs). Moreover, because the core has magnetic property, it is exposed to magnetic field. In addition, Kelvin-Voigt theory is applied to calculate the structural damping of the piezoelectric layers. In order to consider environmental forces applied to structure, the visco-Pasternak model is assumed. In order to consider the mechanical behavior of structure, sinusoidal shear deformation theory (SSDT) is assumed and Hamilton's principle according to piezoelasticity theory is employed to calculate motion equations and these equations are solved based on differential cubature method (DCM) to obtain the vibration and modal loss factor of the structure subsequently. The effect of different factors such as GPLs distribution, dimensions of structure, electro-magnetic field, damping of structure, viscoelastic environment and boundary conditions of the structure on the vibration and loss factor of the system are considered. In order to indicate the accuracy of the obtained results, the results are validated with other published work. It is concluded from results that exposing magnetic field to the MR fluid core has positive effect on the behavior of the system.

• 공업화학
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• 제10권4호
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• pp.548-556
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• 1999

질소 분위기에서 폴리에틸렌 열분해의 속도론적 연구를 $10^{\circ}C/min$와 $50^{\circ}C/min$ 사이의 여러 가지 가열속도에서 비등온 질량감소 기술을 이용하여 수행하였다. 속도 상수들에 대한 정보를 얻기 위하여 질량감소 곡선 및 그 미분값을 Kissinger, Freeman-Carroll, Flynn-Wall, Coats-Redfern, Chatterjee-Conrad, Friedman, Horowitz-Metzger 및 Ozawa 방법과 Denq 방법을 이용하여 해석하였다. 연구에 사용된 수학적인 방법에 따라 속도 상수의 결과값에 많은 차이가 있음을 알 수 있었으며, 따라서 열분해의 속도 상수 결정에 있어서는 각 방법의 장 단점에 대한 비교 분석 연구가 선행되어야 할 것으로 판단되었다. 본 연구로부터 HDPE의 겉보기 활성화 에너지의 값이 LDPE의 값보다 크게 나타냄을 확인할 수 있었다.

• 한국콘텐츠학회논문지
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• 제11권10호
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• pp.242-256
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• 2011

소셜미디어를 통해 개인의 일상을 공유하는 활동이 보편화되었다. 이 경우에는 텍스트뿐 아니라 사진도 많이 활용을 하게 되는데, 사진을 찍어 올리기가 손쉬운 만큼 타인의 사진을 스크랩하는 일도 매우 용이해졌다. 그러나 그 사진이 저작권법상 보호받는 사진저작물에 해당한다면 스크랩에 신중을 기할 필요가 있다. 타인의 사진저작물을 무단이용한 경우 저작권 침해가 성립하며, 이것을 내 블로그 등 소셜미디어에 게시한 경우에는 복제권 전송권 전시권을 침해하게 된다. 스크랩이 허용된 경우라 하더라도 그 사진저작물을 영리목적으로 사용하려면 별도의 허락을 얻어야 한다. 저작권 침해 논란에서 벗어나기 위해 타인의 사진저작물을 변형해서 사용하는 경우가 있는데, 이때에는 변형의 정도에 따라 복제권 동일성유지권 2차적 저작물작성권 침해가 이루어진다. 화면캡처의 기술이 발달하여 몇 번의 클릭만으로 타인의 사진저작물을 다운로드받을 수 있지만, 기술적으로 가능한 행위가 모두 합법적이지는 않다는 사실을 항상 인식하여야 한다.

• 전기전자학회논문지
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• 제24권3호
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• pp.706-712
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• 2020

유기전기발광소자(Organic light diodes, OLEDs)의 전기발광특성을 향상시키기 위해, 본 논문에서는 용액 공정 정공주입층으로 신규 hexaazatrinaphthylene(HATNA) 유도체들을 도입한 고효율 녹색인광 OLED소자의 특성을 연구하였다. HATNA 유도체는 Indium Tin Oxide(ITO)의 일함수와 비슷한 낮은 the lowest unoccupied molecular orbital(LUMO) 에너지 준위를 가져 다른 개념의 정공주입 메커니즘을 보여주었다. HATNA 유도체를 hole injection layer(HIL)로 사용한 OLED소자들은 HTL로의 정공주입장벽을 효과적으로 감소시키고 발광층 내에 전자와 정공의 균형을 최적화 시켜 외부양자효율이 10.8%에서 15.6%로, 전류 효율은 34.3 cd/A에서 42.7 cd/A로 소자 효율이 크게 향상 되었다.