Background: Proper detection and management of dental plaque are essential for individual oral health. We aimed to evaluate the maturation level of dental plaque using a two-tone disclosing agent and to compare it with the fluorescence of dental plaque on the quantitative light-induced fluorescence (QLF) image to obtain primary data for the development of a new dental plaque scoring system. Methods: Twenty-eight subjects who consented to participate after understanding the purpose of the study were screened. The images of the anterior teeth were obtained using the QLF device. Subsequently, dental plaque was stained with a two-tone disclosing solution and a photograph was obtained with a digital single-lens reflex (DSLR) camera. The staining scores were assigned as follows: 0 for no staining, 1 for pink staining, and 2 for blue staining. The marked points on the DSLR images were selected for RGB color analysis. The relationship between dental plaque maturation and the red/green (R/G) ratio was evaluated using Spearman's rank correlation. Additionally, different red fluorescence values according to dental plaque accumulation were assessed using one-way analysis of variance followed by Scheffe's post-hoc test to identify statistically significant differences between the groups. Results: A comparison of the intensity of red fluorescence according to the maturation of the two-tone stained dental plaque confirmed that R/G ratio was higher in the QLF images with dental plaque maturation (p<0.001). Correlation analysis between the stained dental plaque and the red fluorescence intensity in the QLF image confirmed an excellent positive correlation (p<0.001). Conclusion: A new plaque scoring system can be developed based on the results of the present study. In addition, these study results may also help in dental plaque management in the clinical setting.
Background: The use of dental floss is associated with a reduction in dental caries and periodontal disease. According to personal preference, not only thread type but also C type and Y type floss are used. Although the effectiveness of dental floss for removing dental plaque has been proven, plaque removal effect of C type and Y type floss has not been well reported. In this study, the plaque removal effect of C type and Y type floss compared to thread type floss was experimentally verified. Methods: Thread type, C type and Y type floss were used to remove dental plaque. Ten people in each flossing group participated, and by applying dental floss to the 6 incisors of the maxilla and mandible, the degree of dental plaque was analyzed by QLF-D. To evaluate the removal degree of dental plaque before and after flossing, Simple Plaque Score (SPS), Area R30, Area R70, and Area R120 score were measured. Results: In the analysis using the Area R30 fluorescence score of the QLF-D system, the degree of plaque removal according to the application of dental floss was effective in all the thread type (p=0.018), C-type (p=0.012), and Y-type (p=0.012) floss groups compared to before the application of the floss. Among them, C type floss was more effective in removing plaque than thread type and Y type floss. However, the plaque removal effect between the three floss types was not significantly different in ∆SPS (p=0.674), ∆Area R30 (p=0.726), ∆Area R70 (p=0.504), and ∆Area R120 scores (p=0.423). Conclusion: Thread type, C type, and Y type floss were all effective in removing dental plaque, but there was no significant difference in dental plaque removal effect according to the type of floss.
This study aimed to compare the effectiveness of chewable toothbrush and manual toothbrush and provide basic data for recommendation of the chewable toothbrush in specific groups and situations. A total of 20 subjects participated in this study (rolling method, 10; non-rolling method, 10). After professional prophylaxis, participants used the manual toothbrush to brush their teeth for 3 minutes. After a 7-day wash-out period, participants used the chewable toothbrush according to the manufacturer's instructions. Pre- and post-plaque indexing of the teeth was performed. The dental plaque index was assessed using the Turesky Modification of the Quigley-Hein Plaque Index (TMQHPI) for amount of plaque and Silness-Loe Plaque Index (SLPI) for plaque thickness. The difference between pre- and post-dental plaque index was analyzed using a paired t-test and the Wilcoxon signed-rank test. The Mann-Whitney U test was also used to compare the dental plaque index reduction rates. The dental plaque index differed significantly between the chewable toothbrush and the manual toothbrush. The TMQHPI reduction rate was significantly different between the rolling and non-rolling method groups for the manual toothbrush but not the chewable toothbrush. The difference in SLPI reduction rate between the rolling and non-rolling method groups was significant for the manual toothbrush but not for the chewable toothbrush. Differences in the dental plaque index reduction rates between the chewable and manual toothbrushes were not significant in the non-rolling method group. The results of this study showed higher reduction rates in dental plaque with manual toothbrush use than with chewable toothbrush use. However, the non-rolling method group did not show statistically significant differences according to toothbrush type. The present study showed that a chewable toothbrush can be an alternative to a manual toothbrush for individuals who have difficulty using the generally recommended rolling method.
Objectives: Interdental toothbrushes are made and sold in various design types and brush thicknesses. However, there is little research on which type of interdental toothbrush currently manufactured and sold on the market is the most effective in eliminating interdental plaque. Therefore, this study aims at comparing the elimination rate of artificial dental plaque between reciprocating- and rotating-types of interdental toothbrush based on the frequency of application and thickness of brush. Methods: This study focused on the effective management of dental plaque using interdental toothbrush, a recommended item for personal dental hygiene. The method was as follows: artificial dental plaque coloring was applied to the distal surface of artificial tooth #46 and the mesial surface of #47. The area was subject to reciprocal movement three and six times to eliminate artificial plaque. Results: The results showed that using a 0.7mm rotating interdental toothbrush, on the proximal surface of each molar, the elimination rates were: on the distal surface of #46, upon three applications 40.24%, upon six applications 30.41%; on the mesial surface of #47, upon three applications 44.52%, upon six applications 29.72%. Conclusions: These results showed that for rotating-type interdental toothbrushes, a high dental plaque elimination rate was observed even though many reciprocal movements were not performed.
Kim, Ye-Eun;Ko, Seong-Eun;Sa, Da-Eun;Lee, Ji-Eun;Jeon, Se-Yoon;Lim, Do-Seon
Journal of dental hygiene science
/
v.20
no.4
/
pp.245-251
/
2020
Background: This study tries to compare and analyze the removal effect of dental plaque of general dentifrice and pregnant women's dentifrice and quantify the results to provide basic data so that consumers can make reasonable choices when purchasing dentifrice, and also increase interest in the dental plaque. Methods: After forming a dental plaque (carbohydrate porridge) on the labial surface of the bovine teeth, a disclosing agent was applied. Then the same experimenter brushed the surface of the bovine teeth using an electric toothbrush and took photographs using a DSLR camera. Thereafter, the residual amount of dental plaque was analyzed using the ImageJ program, and SPSS 26.0 was used for statistical processing. Results: The average residual amount of dental plaque using the general dentifrice was 11.71% for Perio, 9.45% for Cliden, and 8.47% for 2080, and the average residual amount for the three types was approximately 9.88%. The average residual amount of dental plaque of pregnant women's dentifrice was 13.95% for Jeninmothers, 12.53% for Tntnmoms, and 12.63% for Mommiracle, and the average residual amount of the three types was approximately 13.04%. On comparing the average residual amount of dental plaque between general and pregnant women's dentifrices, it was observed to be 3.16% higher for the latter. However, the results were not statistically significant. Conclusion: According to the research results, there was no significant difference in removal effects of general dentifrice and pregnant women's dentifrice. In addition, when a pregnant woman uses the right toothbrushing method with pregnant women's dentifrice, it can prevent or inhibit the progression of the gestational periodontal disease. Therefore, we recommend pregnant women to use pregnant women's dentifrices.
The use of dentifries in toothbrushing is to aid removal of dental plaque and contribute to good oral hygiene. Therefore, it is important to record the quantity of dental plaque present after toothbrushing. This report concerns with the plaque removal of toothbrushing in a group of dental students who used dentifrices 'X' 'Y' and 'Z' marketed in Korea. And the cleansing effect was examined in accordance with Simplified Oral Hygiene Index. The Following conclusion was obtained. The dentifrices 'X' 'Y' and 'Z' were effective on the removal of dental plaque in the degree of -0.66±0.21, 0.70±0.24 and 0.84±0.19 plaque Index. The differences in the value of plaque Index between the dentifrices 'X', 'Z' and 'Y', 'Z' were significant statistically. The dentifrice 'Z' had the most intensive effects above all.
Dental plaque has been considered as one of the major causes of inflammation. Therefore, removal of dental plaque by tooth brushing is the most important to prevent gingival and periodontal diseases and to maintain postoperative gingival health. The author executed clinical examination on 100 outpatients who were admitted to infirmary of dental college, S.N.U. The patients were examined on their tooth brushing method, frequency, habit and admitted experience to dental clinic. The obtained results were as follows; 1. The patient group who brushed with the method of Stillman, showed the lowest plaque index. 2. The examined group who brushed more frequently per a day, showed the tendency of reduced plaque index. 3. Plaque index was lowest in the group who brushed after meals and before bed. 4. The patient group who visited more frequently to dental clinic, showed the lower tendency of plague index. 5. Among the total examined group, those who recognized the importance of oral hygiene were less than 10%.
This study was executed to evaluate the effects of natural herbal medicine extract on the antimicrobial activity and the artificial plaque formation. Schisandra chinensis, Sophora flavescens, Dryopteris crassirhizoma and Aristolochia contorta were extracted by 80% MeOH respectively and each concentration(5mg, 10mg, 20mg) of herbal medicine extract was tested to show the antimicrobial activity against S. mutans. To show the inhibition effect of herbal medicine extract on the artificial dental plaque formation each extract of 0.4% concentration was added into M 17 broth and inoculated with $5.0{\times}10^6$ of S. mutans. After 24 hour incubation each weight of artificial dental plaque was measured and compared with each other. Results obtained are as follows: 1. Dryopteris crassirhizoma showed very intense antimicrobial effect but Schisandra chinensis, Sophora flavescens and Aristolochia contorta showed a little difference against control. 2. Regarding artificial plaque formation inhibiting activity by each herbal extract at 0.4% concentration, Dryopteris crassirhizoma showed the perfect inhibition effect. Schisandra chinensis and Aristolochia contorta followed in order but Sophora flavescens showed the worst value. In conclusion, Dryopteris crassirhizoma showed strong antimicrobial effect against S. mutans and significant inhibiting effect on the artificial dental plaque formation.
To test flavonoids for antibacterial activity against oral micraorganisms, flavonoids, quercetrin and naringenin, were incorporated into two pharmaceutical preparations in the form of tooth paste. Samplees of dental plaque, the msot accused dental deposit which initiates the gingival and periodental diseases, were collected from the teeth surface of ten dental students at one week interval before and after using placebo, followed by two formulae of tooth paste containing 0.1% of quercetrin and naringenin (formulas I and II, respectively). The amount of dental plaque was assessed by the quigley and Hens index. Then plaque samples were subjected to bacteriological examination of Gram stain and plate counts of microorganisms. The amount of dental plaque was assessed by the Quigley and Hens index. Then plaque samples were subjected to bacteriological examination of Gram stain and plate counts of microorganisms. The results revealed that most of Gram negative cocci and bacilli were highly affected by the two formulae : the number of actinomycetes were decreased after using formula I and disappeared completely by the sue of formula II, while the number of Gram positive streptococci was highly decreased after the treatment with the two formulae. These results indicate a possible use of flavonoids to inhibit dental plaque formation.
Background: Dental caries and periodontal disease are bacterial infectious disease, mainly caused by plaque, a bacterial colony deposited on the tooth surface and gum tissue. Dental plaque disclosants easily stain the dental plaque, making them effective for scaling and tooth brushing education. As the erythrosine typically contained in dental plaque disclosants is highly cytotoxic, a low toxicity additive is needed. In this study, we aimed to examine the natural pigments with negligible cytotoxicity but can effectively stain the dental plaques for use in dental plaque disclosants. Methods: The pigmentation of eight types of natural pigments was tested on bovine tongue and teeth, as well as on head and neck tissue sections of experimental ICR mice. The cytotoxicity of gingival epithelial cells was measured via MTT assay. Pigmentation was performed on the bovine tongue and tooth surface. Pigmentation in the oral environment was observed in four mandibular incisors. A 2 Tone was used as a control. Results: Of the eight types of natural pigments, purple and blue pigments were effective in coloring dental plaques on the enamel surface as well as in the head and neck tissue sections. Additionally, purple and blue pigments were visible on the surface of the bovine tongue. Red, pink, orange, green, purple, and yellow pigments showed strong cytotoxicity, whereas brown and blue pigments had relatively low cytotoxicity. Blue pigment was effective in staining the dental plaque of four mandibular incisors. Conclusion: We suggest that the blue pigment derived from Gardenia jasminoides Ellis (Rubiaceae), which is effective for coloring dental plaques and has low cytotoxicity, is useful as a naturally derived dental disclosant.
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