The purpose of this study was to observe the demineralization effects of commercial drinks on bovine enamel. 12 bovine teeth without dental caries were divided into 3 experimental groups (n = 9) and control groups (n = 3). All the samples immersed in beverages maintained by $37^{\circ}C$ in incubator respectively. The differences of surface microhardness values(${\Delta}VHN$) between before and after 45 hours treatment were $166.97{\pm}51.69$(in Coca-cola), $116.26{\pm}20.81$(in Gatorade), $77.89{\pm}55.88$(in Hite), and $23.44{\pm}29.97$(in Jeju SamDaSoo). Morphological changes by scanning electron microscope were indicated that a sports drink cause significant enamel demineralization effects on the bovine enamel surface among four beverages.
The natural products are used to be development of new antibacterial substances against human pathogenic bacteria. Adherence to the tooth surface by S. mutans is an important step in initiation of dental caries. This study was to examine antibacterial activity and anti-adhesive effect of Scutellaria baicalensis extract against S. mutans. Extracts of S. baicalensis were tested for antimicrobial activities by paper disc methods and radial diffusion assay methods, and bacterial adherence assay using 3 type of hydroxyapatite. The antibacterial level of ethyl acetate extract, IPK-3 on the growth of S. mutans was 125 mg/ml of minimum inhibitory concentration (MIC). The maximum growth of S. mutans in medium added with IPK-3 extract (50 mg/ml) was delayed to 30 hr, while the highest at 24 hr in control medium. The pH values of the control medium was 5.63 at 18 hr, but the media supplemented with IPK-3 extract was pH 6.50 at 12 hr. In adhesive inhibition assay, S. mutans was labelled with the fluorescent indicator DAPI and measured with fluorescence microscope. Adhesion of S. mutans on hydroxyapatite beads was inhibited by IPK-3 extracts. These results suggest that S. baicalensis extract can be used as an effective material for antibacterial activity and adhesive inhibition against S. mutans.
The purpose of this study was to grasp the benefits from pit and fissure sealants and to analyze the factors of influencing the benefit amount by using the raw data of the Korea National Health and Nutrition Examination Survey for 2007~2013 targeting the subjects aged from 6 years in full to 18 years. The analysis was used STATA 11.0. As a result of the analysis, the following conclusions were obtained. It rose up to 1.24 pieces after carrying out the national health insurance coverage from averagely 0.93 piece until the year in 2007~2009 (up to November) when is before enforcing the national health insurance coverage in the pit and fissure sealants. The benefits from pit and fissure sealants depending on general characteristics were surveyed to be higher in the higher income level, in the more subscription to health insurance and private insurance, and in the more toothbrushing frequency per day. The factors that have influence upon the benefits from pit and fissure sealants were shown to include whether or not to have the national health insurance coverage in the pit and fissure sealants, income level, health insurance type and private insurance subscription appearance, and one-day toothbrushing frequency. Synthesizing the results, a rise in the benefits from pit and fissure sealants is shown in 2010 based on December 2009 when the national health insurance coverage in the pit and fissure sealants was implemented, but is showing the tendency of declining again from 2011. To increase the benefits from pit and fissure sealants, it is thought that the schemes will need to be discussed such as reinforcing publicity on the national health insurance coverage in the pit and fissure sealants, expanding a support for low-income bracket, and differentiating the outpatient cost sharing according to socio-economic level.
The esthetic factor for the esthetic prosthesis were studied on 300 persons. The praaticipants were 150 students registered under the Deparment of Dental Laboratoy Technology, Jisan Junior College at the present day of June, 1995 and their mothers. The results were as follows; 1. The number of persons with 28 teeth in their oralcatity was highest(P<0.01). They complained that they have only 28 teeth bacaues of the loss(deficiency) fo teeth due to their impacted teeth and dental caries. 2. The presons with feelings of dissatisfaction were large in number(P<0.01). They were dissatisfied with an abnormal tooth form(33.5%), tooth position(31.7%), tooth color(31.1%), and tooth size(3.7%) in order(P<0.01). 3. In their facial form, the most numerous were men with square form and women with ovoid form(P<0.01). Among the whloe number fo facial forms it appeared to decresae in the order of ovoied(41.8%), square(32.7%), square+tapered(20.5%), and tapered form(5%)(P<0.01). In a profile form, the most numerous were men with A form and women with B. Among the whole participants the most numerous were persons with B form and persons with A and C form were next in oder of numbers(P<0.01). 4. In discoloration of teeth, the most numerous were persons who have no discoloration. 5. In the esthetic recovery of anterior prosthesis the color matching of proximal teeth was not correct, but the outline of proximal teeth was to be reproduced correctly. 6. In the diastema of teeth, the number of persons who have no diastema was higher than that of persons who have a disatema(P<0.01) Among the persons who have diastema the most numerous were persons who have a diastema, and next were 2, 4, 3 and 5 diastemas in order. 7. In a meeting point of the midline of the body and the tooth the number of person hanving a meeting point was higher than that of persons who did not fit each other(P<0.01). The bias direction of midline teeth appeared to be affected by chewing direction, prosthesis location, and posterior location. 8. The length and width of teeth in the tooth size were suitable(P<0.01). The number of persons who have central teeth longer than lateral teeth was high(P<0.0). 9. In the results of smile analysis, the line form connected with incisal edge of upper canine were parallel(P<0.01). When smile the location fo upper lips should be in accord with the central area of teeth(P<0.01) and lower lip should be atteched to the edge of anterior teeth in order to be esthetic. 10. Among the number of upper teeth we can see the most numerous were 8 teeth and next were 10, 6 and 12 in order.
Journal of the korean academy of Pediatric Dentistry
/
v.27
no.4
/
pp.549-557
/
2000
Dental caries is a bacterial disease of the dental hard tisssus, characterized by a localized, progressive, molecular disintegration of tooth structure. The action of Leuconostoc lactis 51 about plaque formation and replication by Streptococcus mutans was studied as follows. 1. Lower amount of plaque was produced at the mixed culture of S. mutans and L. lactis 51 than S. mutans alone on the wires in the beaker. 2. Fewer cells of S. mutans were replicated at the mixed culture of S. mutans and L. lactis 51 than S. mutans alone. 3. In M17Y broth, viable cells of S. mutans and L. lactis 51 increased for 12 hours, and decreased for 24 hours. In M17YS broth, viable cells of S. mutans showed time-dependent decrease at mixed culture of S. mutans and L. lactis 51. 4. The culture supernatant of L. lactis 51 didn't inhibit the replication of S. mutans and the formation of artificial plaque. 5. Sucrose and frutose were extracted from the culture supernatant of L. lactis 51 in M17YS broth. These results suggest that L. lactis 51 isolated from the oral cavity inhibits the replication of S. mutans and the formation of artificial plaque.
Journal of the korean academy of Pediatric Dentistry
/
v.29
no.1
/
pp.92-99
/
2002
There have been efforts that inhibit development of dental caries by sugar substitution. But, it is controversial if xylitol has anticariogenic effect in the presence of sucrose. And there are few papers dealing with the combined action of xylitol and sucrose. For the purpose of resolving this controversy, the author investigated the effect of xylitol on enamel demineralization and on adhesiveness of S. mutans to hydroxyapatite in the presence of sucrose. Five experimental solutions were prepared as follows: (S: sucrose, X: xylitol) Group 1: BHI broth Group 2: BHI+1% S Group 3: BHI+0.75% S+0.25% X Group 4: BHI+0.5% S+0.5% X Group 5: BHI+0.25% S+0.75% X Group 6: BHI+1% X Each solution was inoculated with $100{\mu}l$ of S. mutans JC-2. And saliva coated hydroxyapatite beads were put into each experimental solution. And then each solution was incubated at $37^{\circ}C$ under anaerobic condition. After incubation, the adhesiveness of S. mutans on hydroxyapatite was evaluated. The Vickers hardness numbers were measured on extracted human primary teeth, and these teeth were dipped into the same experimental solution and incubated at $37^{\circ}C$ under anaerobic condition for 48hours. Surface microhardness were measured again after incubation. The obtained results were as follows; 1. In the presence of sucrose, xylitol can reduce the adhesiveness of S. mutans on hydroxyapatite surface from the ratio of 25% sucrose to 75% xylitol(P<0.05). 2. In the presence of sucrose, xylitol can reduced demineralization of primary teeth enamel surface from the ratio of 50% sucrose to 50% xylitol(P<0.01).
Objectives : Yongdamgosam-hwan(YGH) has been used as a traditional medicine from old times for antiinflammatory effects. Streptococcus mutans(S. mutans) is known as a prime bacteria responsible for causing caries by forming a biofilm referred to as dental plaque on the tooth surface. But antimicrobial activity of YGH with dental disease is not sufficiently understood. This study was designed to investigate the effects of YGH ethanol extract on antimicrobial effect against Streptococcus mutans.Methods : The antimicrobial effect of YGH ethanol extract was assessed by the paper disk diffusion method and optical density method to determine minimum inhibition concentration(MIC), also observed by fractional inhibitory concentration index(FICI) and time-kill assay to figure out the synergic effect on the combination of YGH ethanol extract with antibiotics.Results : The YGH ethanol extract 500 μg was 7.5-8.5 mm diameter of clear zone of inhibition against Streptococcus mutans in a concentration-dependent manner and MIC was 250 μg/mL. The administration of the ethanol extract in combination with gentamicin and streptomycin induced a reduction of ≥4-8-fold in all tested bacteria. Furthermore, time-kill study was found that a combination of YGH ethanol extract with oxacillin and streptomycin produced a more rapid decrease in the concentration of bacteria CFU/mL than the YGH ethanol extract or antibiotics alone.Conclusions : As a result, the YGH ethanol extract has good antimicrobial effects. And the results suggest that YGH could be employed as a natural antibacterial agent in dental care products.
Journal of the Korea Academia-Industrial cooperation Society
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v.14
no.1
/
pp.206-213
/
2013
This study aims at finding which change there is of oral health promotion as investigating the oral health status in quality, diagnosing which effects knowledge and attitude of oral health has before and after the oral health education as developing and conducting the oral health education program for international marriage migrant women to form their oral health belief for improving oral health. This study consisted of each 51 of the experimental group and the controlled group at the multi-cultural family support center from $26^{th}$ of March, 2012 to $30^{th}$ of June, 2012 as the subjects, and pre-to-post investigated knowledge of oral and cognition. Also as it took follow-up examination of the clients who visited to the dentist with changing of their cognition, conducted matched-pair sample t-test and analysis of repeated measure variance. As the result, there were always the changes at the field of knowledge about oral, periodontal disease and toothbrush in awareness of oral health, and at the field of periodontal disease, dental caries, toothbrush, fluorine and bad breath in knowledge of oral health. The change of DMFT index, DT index has been reduced and FT index has been increased. As the result above, the oral health education program for international marriage migrant women has led to change awareness of oral health and knowledge, and the change of knowledge has influenced to a behavior, so there were the changes of periodontal status and DMFT index as well. This has been showing the importance of the program for oral health of international marriage migrant women. Moreover, while the oral health education program is developed in various aspects by offering the information for developing the oral health education program in future, it needs to run parallel prevention with treatment.
Background: This study aimed to explore the overall perception of pit and fissure sealants and suggest methods to revitalize their current stagnation. Methods: To determine the social perception of the change in coverage policy for pit and fissure sealants, we categorized them into five time periods. The first period (December 1, 2009 to November 30, 2010), the second period (December 1, 2010 to September 30, 2012), the third period (October 1, 2012 to May 5, 2013), the fourth period (May 6, 2013 to September 30, 2017), and the fifth period (October 1, 2017 to December 31, 2022). We utilized text mining, an unstructured big data analysis method. Keywords were collected and analyzed using Textom, and the frequency analysis of the top 30 keywords, structural features of the semantic network, centrality analysis, QAP correlation analysis, and co-occurrence analysis were conducted. Results: The frequency analysis showed that the top keywords for each time period were 'Cavities', 'Treatment', and 'Children'. In the structural features of the semantic network of pit and fissure sealants by time period, the density index was found to be around 1.00 for all time periods. The QAP correlation analysis showed the highest correlation between the first and second periods and the fourth and fifth periods with a correlation coefficient of 0.834. The co-occurrence analysis showed that 'cavities' and 'prevention were the top two words across all time periods. Conclusion: This study showed that pit and fissure sealants are well accepted by the society as a preventive treatment for caries. However, the awareness of health education related to these sealants was found to be low. Efforts to revitalize stagnant pit and fissure sealants need to be strengthened with effective education.
For the detection of the active cariogenic factors contributing to caries development, some practical methods such as the Snyder test, estimation of salivary flow rate and salivary buffering capacity test were evaluated statistically by comparing DMFT and DMFS indexes. Total 122 children (62 male and 60 female ; 64 rural and 58 urban) were selected ramdomly from the 6th grade of the primary school and their salivary cariogenic factors were analysed and evaluated. Among the total 122 children, 78.7% was positive in the snyder test in which the marked, moderate and slight caries activities were 29.5%, 30.3% and 18.9%, respectively. In the Snyder test, 74.45% was positive in urban children while 84.48% was positive in rural children. DMFT and DMFS indexes were markedly lower in negative group than positive group of the Snyder test (p<0.01). The mean and standard deviation of stimulated salivary flow rate was 6.97$\pm$2.57 in male and 6.34$\pm$2.54 in female but no significant difference was observed in sexuality. The stimulated salivary flow rate of urban children was slightly higher that of rural but there was no significant difference between them. However, the group that showed below average in the stimulated salivary flow rate was markedly higher in DMFI and DMFT indexes than the group of above average. The mean and standard deviation of stimulated salivary buffering capacity was 7.65$\pm$2.19 in male and 6.80$\pm$1.67 in female. This difference was significant statistically(p<0.05). Stimulated salivary buffering capacity of urban children was higher than that of rural. Increases in stimulated salivary flow rate and buffering capacity had reduced the onset of dental caries of 14-year-old permanent tooth.
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