• 한국균학회지
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• 제20권1호
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• pp.51-57
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• 1992

Mitochondria in Pleurotus ostreatus were isolated and purified by stepped sucrose density gradient centrifugation, to compare the effects of organic compound on the activities of mitochondrial ATPase in Basidiomycotina with those in mammalian cell. The effects of N, N'-dicycio-hexylcarbodiimide (DCCD), carbonyl cyanide m-chlorophenylhydrazone (CCCP), sodium azide and aurovertin known as compounds to be related to electron transfer system in mitochondria were studied. A activity of mitochondrial ATPase was inhibited by 64%, 57% and 53% in the presence of 0.25 mM DCCD, 0.02 mM sodium azide and 1.5 $({\mu}g/mg\;of\;protein)$ aurovertin B, respectively. It was stimulated by 22% in the presence of 0.15 ${\mu}M$ CCCP.

• 한국가시화정보학회:학술대회논문집
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• 한국가시화정보학회 2005년도 추계학술대회 논문집
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• pp.69-73
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• 2005

Three-dimensional density distributions of an impinging and eccentric flame have been analyzed numerically and experimentally by a combined optical system with a digital speckle tomography. The flame has been ignited by premixed butane/air from air holes and impinged vertically against a plate located at the upper side of tile burner nozzle. In order to compare with experimental data, computer synthesized phantoms of impinging and eccentric flames have been made and reconstructed by a developed three-dimensional multiplicative algebraic reconstruction technique (MART). A new scanning technique has been developed for the analysis of speckle displacements to investigate wall jet regions of the impinging flame including sharp variation of the flow direction and pressure gradient. The reconstructed temperatures have been compared with a temperature photography by an infrared camera and results of numerical analysis using a finite-element method.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2006년도 추계학술대회
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• pp.28-31
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• 2006

It is require to construct geothermal database to develop geothermal energy as renewable energy policy. It must be consist of geologic data, borehole data and geophysical data for geothermal database. In aspect of geology, there are included the distribution of geology, structural geology, geological time, rock name, density of rock, porosity, thermal diffusivity, specific capacity and thermal conductivity In order to calculate the heat general ion, it is needed to analysis the radioactivity elements as U, Th and K of rock. In aspect of borehole data, there are included temperature of depth, surface temperature and geothermal gradient And also there is geotherrnornetry using chemical components of groundwater as Na Ca, K and $SiO_2$. In aspect of geophysical data, there are some thematic map as booger gravity anomaly data and magnetic survey data and etc. In addition, it is important to descript the distribution of hot spring and water temperature.

• Applied Microscopy
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• 제11권1호
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• pp.29-38
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• 1981

Bacteriophage f2 were treated with ozone at various concentrations for 20 minutes. The inactivation kinetics of f2 phage were examined during ozonation. In order to study the mode of action of ozone on the phage f2, absorption of the phage to the host pili was meassured by utilyzing radioactivity of tritium incorporated into the phage RNA. Sucrose density gradient analysis and electron microscopy were also used to prove the mechanism of ozone inactivation of the phage. Strucural proteins of the phage were broken by ozonation into many protein subunits. The extent of phage breakage was proportional to ozone concentration and reaction time. Percent decrease of the phage absorption to the host pili was coincident with the rate of ozone inactivation of the phage. Ozone inactivation of bacteriophage f2 was shown to be caused by the breakage of the structural protein and blockage of the phage absorption to the host pili.

• 설비공학논문집
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• 제10권2호
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• pp.217-226
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• 1998

A numerical study has been carried out for two- and three-dimensional buoyant turbulent flow in a stairwell model. The Reynolds-averaged Navier-Stokes and energy equations are solved with the authors'own computer program. Two models by the Boussinesq approximation and the density-gradient form are used for buoyancy terms in the governing equations. Two- and three-dimensional predictions of the velocity and temperature fields are presented and the results are compared with experimental data. Comparisons have also been made in detail with two-dimensional predictions. Two-dimensional and three-dimensional simulations have predicted the overall features of the flow satisfactorily. A better agreement with experiment is achieved with three-dimensional simulations.

• Nuclear Engineering and Technology
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• 제30권1호
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• pp.17-25
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• 1998

Transport of carbon and boron impurity ions parallel to magnetic field lines in the tokamak SOL (scrape-off layer) is numerically investigated for a one-dimensional steady state. The spatial distributions of density and velocity of the impurity ions in a steady state are calculated by finite difference method for a single-fluid model. The calculated results show that among forces acting on SOL particles thermal force produced tv plasma temperature gradient is a principal force determining the feature of impurity distribution profiles in the tokamak edge. However, strong collisional friction forces appearing dominant in front of the diverter plate restrain impurity ion flows due to temperature gradients from moving toward the midplane. Consequently, the stagnation point develops in the impurity flow by these two forces near the diverter region, in which ion flows change their directions. Impurity ions turn out to be accumulated at the stagnation points, where peaked profiles of highly-ionized state ions are relatively predominant over those of low-ionized state ions.

• 미생물학회지
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• 제31권3호
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• pp.184-188
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• 1993

Novel Ustilagomaydis strains, designated as SH1 to 14 containing new types of ds RNA segments, are identified from corn smut in Korea. Among 14 isolates, 7 isolates appear to posses virus particles and the other isolates may contain dsRNA as a plasmid form. The pattern of dsRNA is highly diverse form a typical P-type containing one or more of H, M, and L dsRNAs to the one containing one or move M dsRNAs. It is likely that the strains containing H dsRNA posses virus particles which were confirmed by sucrose density gradient followed with different range of specificity and the activity of the strain (SH14) is stronger than A4 toxin. The sensitivity of 14 isolates is also very diverse and two strains (SH10, SH11) appear tobe universal sensitve strains against 5 tested toxin samples.

• Bulletin of the Korean Chemical Society
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• 제34권3호
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• pp.743-748
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• 2013

The temperature dependence of the partition coefficients of a neuropeptide, substance P (SP), in isotropic acidic bicelles was investigated by using a pulsed field gradient nuclear magnetic resonance diffusion technique. The addition of negatively charged dimyristoylphosphatidylserine to the neutral bicelle changed the SP partitioning a little, which implies that the hydrophobic interaction between the hydrophobic residues of SP and the acyl chains of lipid molecules is the major interaction while the electrostatic interaction is minor in SP binding in a lipid membrane. From the temperature dependence of the partition coefficients, thermodynamic functions were calculated. The partitioning of SP into the acidic bicelles is enthalpy-driven, as it is for small unilamellar vesicles and dodecylphosphocholine micelles, while peptide partitioning into a large unilamellar vesicle is entropy-driven. This may mean that the size of lipid membranes is a more important factor for peptide binding than the surface curvature and surface charge density.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2010년 춘계학술대회논문집
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• pp.534-541
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• 2010

A high-order accurate Euler flow solver based on a discontinuous Galerkin method has been developed for the numerical simulation of unsteady flows on unstructured meshes. A multi-level solution-adaptive mesh refinement/coarsening technique was adopted to enhance the resolution of numerical solutions efficiently by increasing mesh density in the high-gradient region. An acoustic wave scattering problem was investigated to assess the accuracy of the present discontinuous Galerkin solver, and a supersonic flow in a wind tunnel with a forward facing step was simulated by using the adaptive mesh refinement technique. It was shown that the present discontinuous Galerkin flow solver can capture unsteady flows including the propagation and scattering of the acoustic waves as well as the strong shock waves.

• 대한자기공명의과학회:학술대회논문집
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• 대한자기공명의과학회 2001년도 제6차 학술대회 초록집
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• pp.113-113
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• 2001

Purpose： In the gradient echo EPI the conventional T2＊-weighted image is poor in signal as well distorted by the field inhomogeneity. By acquiring a proton density image in addition to th T2＊-weighted image at the same scan, the fMRI processing can be improved. Method： The central region of the k space is acquired twice at different time points after th RF pulse while acquiring the other regions onc as described in Fig. 1. In Fig. 1 the segment numbers are chronological. Then, we can get two images of different contrast by interleaving th central region in the k space as done in the dua contrast FSE.