• 한국축산식품학회지
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• 제31권6호
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• pp.851-857
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• 2011

This study was conducted to develop a real time reverse transcriptase-PCR (RT-PCR) method for the detection of viable Enterobacteriaceae in milk using primers based on the genes of ribosomal proteins S11 and S13 and to determine effects of heating and subsequent treatments on the threshold cycle (Ct) of the real time RT-PCR. Total RNA was isolated from 17 strains of bacteria including 11 strains of Enterobacteriaceae suspended in milk using a modified Tri reagent method. SYBR Green Master Mix was added to the RNA and the mixture was subjected to the real time RT-PCR. The Cts of eleven type strains of the Enterobacteriaceae in milk ($10^7$ cells) in the real time RT-PCR ranged from 21.5 to 24.6. However, the Cts of Pseudomonas fluorescens, Acinetobacter calcoaceticus, and three gram-positive bacteria were more than 40. The real time RT-PCR detected as low as $10^3$ cells in agarose gel electrophoresis. The Cts increased from 22.0 to 34.2 when milk samples contaminated with Escherichia coli ($10^7$ cells/mL) were heated at $65^{\circ}C$ for 30 min. In addition, subsequent incubation at $37^{\circ}C$ for 6 and 24 h increased the Cts further up to 36.2 and 37.2, respectively. Addition of RNase A to the bacterial suspension obtained from the heated milk and subsequent incubation at $37^{\circ}C$ for 1 h increased the Cts to more than 40. The results of this study suggests that pretreatment of bacterial cells heated in milk with RNase A before RNA extraction might enhance the ability to differentiate between viable and dead bacteria using real time RT-PCR.

• Asian-Australasian Journal of Animal Sciences
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• 제21권1호
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• pp.29-34
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• 2008

The aim of the study was to investigate the effect of dietary fish oil supplementation on the semen characteristics of the Markhoz buck. Sixteen bucks were randomly allocated into 4 groups and received four different diets: unsupplemented control diet, supplemented with fish oil at 2.50% dry matter (DM), supplemented with fish oil (2.50% DM) and vitamin E (0.30 g/kg DM), and supplemented with vitamin E (0.30 g/kg DM). All experimental diets were formulated according to AFRC (1998). Semen was collected at 14 d intervals from June 17, 2006 to September 2, 2006. Semen characteristics were evaluated. Significant effects (p<0.05) of the week (sampling time) were observed for all parameters except semen volume. Also a significant effect (p<0.05) of dietary treatment was observed for all parameters except for percent sperm with normal morphologies and semen volume. Fish oil supplementation with excess vitamin E had a significant effect (p<0.05) on total number and sperm density, motility and progressive motility, percentage viability and dead sperm. The interaction between fish oil feeding and sampling time was significant (p<0.05) for all of the parameters. The bucks that received fish oil in association with vitamin E, effect fish oil showed the greatest improvement in semen characteristics compared with the other groups (p<0.05). This study showed that fish oil supplementation with vitamin E may have a beneficial effect on the semen quality and fertility of Markhoz bucks.

• Asian-Australasian Journal of Animal Sciences
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• 제15권12호
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• pp.1702-1707
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• 2002

The effects of dark-control (D) and continuous green light (GL) exposure of incubated meat-type breeder eggs (Hybro) on embryonic growth from 5 to 15 days of age, hatching time, hatchability per cent and chick hatching weight were investigated in three consecutive experiments at 33, 38, and 41 weeks of age. A total of 798 eggs were used in this study. Eggs were set in an incubator on trays either in the D or under two tubes of 20-watt green fluorescent light during the first 18 days of incubation. Eggs from both treatments were transferred to the dark hatching compartment at 19 days of incubation. The light intensity was in the range of 1,340 to 1,730 lux at the surface of the eggs. GL incubation of eggs significantly (p<0.01) increased weight (expressed as an absolute value) and daily weight gain of embryos at 11 and continued to 15 days of age, hatchability per cent by 4.8%, reduced dead embryos per cent and chick weight at hatch by 37 and 2%, respectively and accelerated hatching time by about 24 h when compared with the D-control incubation. Chicks hatched at 504 h of incubation had significantly (p<0.01) higher body weight, expressed as an absolute value or as a percentage of egg weight, than those hatched earlier at 456 h of incubation. It was concluded that the GL incubation of meat breeder eggs reduced incubation period and chick weight at hatch and increased embryonic growth and hatchability per cent.

• International Journal of Concrete Structures and Materials
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• 제7권1호
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• pp.79-93
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• 2013

One of the biggest problems affecting bridges is the transverse cracking and deterioration of concrete bridge decks. The causes of early age cracking are primarily attributed to plastic shrinkage, temperature effects, autogenous shrinkage, and drying shrinkage. The cracks can be influenced by material characteristics, casting sequence, formwork, climate conditions, geometry, and time dependent factors. The cracking of bridge decks not only creates unsightly aesthetic condition but also greatly reduces durability. It leads to a loss of functionality, loss of stiffness, and ultimately loss of structural safety. This investigation consists of field, laboratory, and analytical phases. The experimental and field testing investigate the early age transverse cracking of bridge decks and evaluate the use of sealant materials. The research identifies suitable materials, for crack sealing, with an ability to span cracks of various widths and to achieve performance criteria such as penetration depth, bond strength, and elongation. This paper also analytically examines the effect of a wide range of parameters on the development of cracking such as the number of spans, the span length, girder spacing, deck thickness, concrete compressive strength, dead load, hydration, temperature, shrinkage, and creep. The importance of each parameter is identified and then evaluated. Also, the AASHTO Standard Specification limits liveload deflections to L/800 for ordinary bridges and L/1000 for bridges in urban areas that are subject to pedestrian use. The deflection is found to be an important parameter to affect cracking. A set of recommendations to limit the transverse deck cracks in bridge decks is also presented.

• 대한한방내과학회지
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• 제25권2호
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• pp.214-223
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• 2004

The study examines the anti-cancer effects of the hot water extract of Mylabris phalerata(MP) using SNU-C5 cell lines. Microscopic analysis showed that 12 hours after MP treatment, the number of dead cells increased prominently. Significant cell death was observed 12, 24, and 48 hours after MP treatment through trypan blue exclusion testing. This suggests that MP is time-dependently cytotoxic. Mitotracker Red CMXRos staining and flowcytometry revealed that MP decreased mitochondrial membrane potentials. The absence of peaks on PI staining showed that DNA damage occurred in MP treated cells. Taken together, measurements suggest that MP has a strong anti-cancer effect on SNU-5 cell lines, and that this is likely to be due to the destruction of mitochondria and DNA damage.

• Asian-Australasian Journal of Animal Sciences
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• 제14권2호
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• pp.184-188
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• 2001

In a summer study during May to July, involving 12 young Murrah buffalo bulls at forty months of average age, the effects of multiple shower vs single shower body cooling and vitamin A, D and E supplementation on the sexual behaviour, semen quality and freezability were investigated. The animals were divided into two groups (6 animals in each group) and housed in a half-walled shed with proper spacing, the feeding management being identical. The bulls in the control group were given a single shower at 1000 h, whereas the experimental bulls were given four showers at 10,12,14 and 16 h. In addition, the experimental bulls were given vitamin A, D and E injections at fifteen day intervals. The sexual behaviour of bulls was observed in terms of reaction time, sexual aggressiveness and ejaculatory thrust. Semen quality of all the bulls was assessed in terms of volume, mass activity, live-dead sperm and sperm concentration, sperm motility and morphology, and acrosomal abnormality. The sexual behaviour did not vary significantly between the groups, whereas semen quality differed significantly for volume, per cent live sperms, total sperms per ejaculate and total live sperm per ejaculate between groups. It can be concluded that sexual behaviour was not influenced by the thermal comfort treatment coupled with periodic vitamin A, D and E injections. But the treatments improved most of the seminal traits in the experimental group of bulls. However, benefit of treatment was not reflected in the freezability traits of the semen.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2018년도 전력전자학술대회
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• pp.144-146
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• 2018

In order to meet the harmonics standards such as IEEE 519 and P1547 the output quality of a grid connected inverter should satisfy a certain level of Total Harmonic Distortion (THD) value. However, the output quality of an inverter gets degraded due to the grid voltage harmonics, the dead time effects and the nonlinearity of the switches, which all contributes to a higher THD value of the output. In order to meet the required THD value for the inverter output under the distorted grid condition the use of harmonic controller is essential. In this paper a novel feedforward harmonic compensation method is proposed in order to effectively eliminate the low order harmonics in the inverter current to the grid. In the proposed method, unlike the conventional harmonic control methods, the hamonic components are directly compensated by the feedforward terms generated by the PR controller with the grid current in the stationary frame. The proposed method is simple in implementation but powerful in eliminating the harmonics from the output. The effectiveness of proposed method is verified through the PSIM simulation and the experiments with a 5kW single phase grid connected inverter.

• 전력전자학회논문지
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• 제24권3호
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• pp.215-227
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• 2019

Grid-connected inverters should satisfy a certain level of total harmonic distortion (THD) to meet harmonics standards, such as IEEE 519 and P1547. The output quality of an inverter is typically degraded due to grid voltage harmonics, dead time effects, and the device's turn-on/turn-off delay, which all contribute to increasing the THD value of the output. The use of a harmonic controller is essential to meet the required THD value for inverter output under a distorted grid condition. In this study, an improved feedforward harmonic compensation method is proposed to effectively eliminate low-order harmonics in the inverter current to the grid. In the proposed method, harmonic components are directly compensated through feedforward terms generated by the proportional resonant controller with the grid current in a stationary frame. The proposed method is simple to implement but powerful in eliminating harmonics from the output. The effectiveness of the proposed method is verified through simulation using PSIM software and experiments with a 5 kW single-phase grid-connected inverter.

• 한국운동역학회지
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• 제12권2호
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• pp.215-227
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• 2002

본 연구의 목적은 정상인 휠체어 농구 선수 8명을 대상으로 추진 및 방향전환 동작의 소요시간 측정 및 운동학적 분석을 통하여 포지션별 농구용 휠체어가 추진동작에 미치는 효과를 규명하는데 있다. 포지션별(포드, 센타, 가드) 휠체어의 한 주기(핸드림 접촉에서 다음 핸드림 접촉까지)를 2차원 DLT 방식을 이용하여 구하고자 하는 변인을 산출하였다. 가드용 휠체어는 직선 구간 및 방향전환 구간 모두에서 가장 빠르게 나타났으며, 센타용 휠체어는 방향전환 구간에서 제일 느린 것으로 나타났다. 이동 속도가 가장 빠른 가드용 휠체어는 손이 TDC(Top Dead Center)에 더 가깝게 접촉하며, 더 오랫동안 핸드림에 힘을 가해서 추진하며, 분당 추진빈도는 가장 적은 것으로 나타났다. 반면에 센타용 휠체어는 손이 TDC에서 가장 멀리 접촉하며, 가장 짧은 범위에서 핸드림에 힘을 가해서 추진하는 것으로 나타났다. 가드용 휠체어는 핸드림 접촉시 팔꿈치를 가장 많이 굽히고 몸통을 가장 많이 세우며, 센타용 휠체어는 핸드림 접촉시 팔꿈치를 가장 많이 펴고 몸통을 많이 굽히며 핸드림 이지시 가드용 휠체어에 비해 팔꿈치를 많이 펴는 것으로 나타났다. 휠체어 추진시 좌석의 높이가 가장 낮은 가드용 휠체어는 포드용 및 센타용 휠체어보다 팔꿈치 및 몸통각의 범위가 가장 큰 것으로 나타났다. 몸통 최대 각속도에서 가드용 휠체어는 다른 휠체어와 비교해서 가장 큰 값을 나타냈다.

• Parasites, Hosts and Diseases
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• 제26권2호
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• pp.95-106
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• 1988

아메바의 cytolytic process가 주로 가수분해 효소 환성과 관계 깊다는 연구들이 70년대 후반부터 병원성 Entamoeba sp.와 Naegleria sp.에서 보고되었지만 Acanthamoeba sp.에서는 가수분해 효소 환성에 관한 연구는 찾아볼 수 없다. 본 연구에서는 병원성이 없는 것으로 알려진 Acanthamoeba royreba를 대조로 하여 병원성이 있는 A. culbertsonl이 가수분해 효소 활성도를 측정하고 이들을 비교하고자 하였다. A. culbertsoni를 마우스에 감염시켰더니 15일내에 마우스 모두가 사망하였으며, 평균 생존기간은 8.5일이었고, 아베바성 수막뇌염으로 사망했음을 알 수 있었다. 반면에 A. royreba를 감염시킨 마우스에서는 수막뇌염이 발생되지 않았다. CGV배지에서 두 종(種) 아메바를 배양하여 아메바 추출물 및 배양액에서 가수분해 효소의 환성을 측정한 결과 A. rcyreba에 비해 A. culbertsoni 세포의 추출물 및 배양액에서 현저히 활성도가 높은 가수분해 효소는 acid phosphatase, p-N-acetyl galactosaminidase, β-N-acetyl glucosaminidase, α-mannosidase, neutral proteinase, acid proteinase였다. 표적 세포인 CHO 세포에 혼합한 A. culbertsoni는 대조군에 비해 강한 세포독성을 나타내었고 CHO 세포와의 혼합후 48시간 후 80%, 72시간후 95% 이상의 CHO 세포가 사멸하는 것을 알 수 있었다. 반면에 A. royreba를 혼합하였을 때는 별다른 세포독성을 나타나지 않았다. CHO 세포에 혼합한 A. tulbgrtsoni이 가수분해 효소 활성도를 경과 시간별로 측정하였던 바 CGV 배지에서 배 양시간이 지남에 따라 아메바내보다 그 배양액에서 더 높은 효소 활성도를 나타내었던 acid phosphatase, β-N·acetyl galactosaminidase, β-N·acetyl glucosaminidase, α-mannosidase, acid proteinase는 혼합 120시간 동안 아메바내에서 더 높은 효소 활성도를 나타내었으며, neutral proteinase의 활성도는 경과시간이 지남에 따라 아메바내에서는 일정하였지만 그 배양액인 EBSS용액내에서는 점점 높아짐을 알 수 있었다. 이상의 성적으로 보아 마우스에서 수막뇌염을 일으키는 A. cuzbertsoni는 비 병원성인 A. reyreba와 비교할 때 여러 종류 가수분해 효소들의 활성도에 현저한 차이를 나타내고 있음을 알 수 있었다. 또한 표적세포인 CHO세포에 대한 A. cuzbertsoni의 세포독성은 강함을 알 수 있었고, 이 때 몇 종류 가수분해 효소들의 환성도도 높게 나타났으며, 이러한 소견이 A. culbertsoni의 병원성과 관련이 있을 것으로 추측되었다.