• Title/Summary/Keyword: deactivated

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Activation of MAP Kinase during Maturation in Porcine Ooctyes (돼지 미성숙란의 체외배양시 MAP Kinase의 활성)

  • 장규태;박미령;윤창현
    • Korean Journal of Animal Reproduction
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    • v.22 no.3
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    • pp.265-276
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    • 1998
  • In an attempt to evaluate the function of MAP kinase of porcine oocytes and to develop a method of assessment for kinase activity, we used MBP as a substrate to detect the MAP kinase activity of porcine oocytes matured in in vitro. The MAP kinase which had lower activity during the first 20 hours of culture started to show an increased amount of activity at 25 hours at which a collapse in nuclear membrane was induced. Significant (P<0.05) a, pp.ared at 30 hours of being cultured. The gel phosphorylation method, MBP which has been known to be a substrate for kinase such as cdc2 kinase, was phosphorylated at two positions corresponding to ERK 1 (44kDa) and ERK2 (42 kDa) which are known as mammalian MAP kinase. The existence of MARKK and MAP kinase were identified with western blotting at 0 hour culture of immature GV oocytes. The amount of those proteins did not increase during 40 hours of culture, which suggest that the increase of MAP kinase activity was caused by phosphorylaton rather than due to change in protein amount. MAPKK and MAP kinase were shown to be dephosporylated with deactivated at M 1 stage by inhibition of protein synthesis with cycloheximide added at the strat following the cultrue. We have reulsts that indicate the existedence of MAP kinase cascade which was activated simultaneously with start of porcine oocyte maturation (GVBD).

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A Study on the Effect of Gallotannin Treatment of Ceramide-containing Fibers on Atopic Skin Diseases (세라마이드 함유 섬유의 복합갈로탄닌 처리에 의한 아토피성 피부질환 완화작용에 대한 연구)

  • Kim, Taekyeong;Cho, Nayoung;Ma, Heejung;Yang, Gwang Wung;Rho, Yong Hwan
    • Textile Coloration and Finishing
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    • v.25 no.4
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    • pp.271-278
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    • 2013
  • In order to investigate the effect of gallotannin treatment to ceramide-containing fabrics on atopic skin diseases, the agglomeration of standard protein BSA and the deactivation of model enzyme were examined. The gallotannin treated on ceramide-containing fabrics precipitated the standard protein, BSA, and therefore deactivated the model enzyme by 70% at 6% treatment concentration. Wash durability should be improved after around 5 cycles of washing. Clinical test of the gallotannin-treated fabrics was carried out on mice for two test items, transepidermal water loss assay and severity score of diseased skin of mice. The results showed significant level of improvement of atopic skin diseases compared with the negative controled.

Effect of Inlet Valve Angle on In-Cylinder Swirl Generation Characteristics(I) (흡입밸브 각이 실린더 내 와류 발생 특성에 미치는 영향(I))

  • Ohm, In-Yong;Park, Chan-Jun
    • Transactions of the Korean Society of Automotive Engineers
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    • v.16 no.6
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    • pp.148-156
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    • 2008
  • This paper is the first of 2 companion papers which investigate in-cylinder swirl generation characteristics according to inlet valve angle. Two DOHC 4 valve engines, one has wide intake valve angle and the other has narrow valve angle, were used to compare the characteristics of swirl motion generation in the cylinder. One intake port was deactivated to induce swirl flow. A PIV (Particle Image Velocimetry) was applied to measure in-cylinder velocity field according to inlet valve angle during intake stroke. The results show that the stronger swirl motion is observed in wide valve angle engine at the early intake stage; however, the swirl motion is gradually distorted by the intake flow component passing through valve area near the cylinder wall as the stroke proceeds. The tumble motion also does so in wide angle. On the contrary, the swirl and tumble motions, which are not clear at the initial stage, become better and better arranged as the piston goes down and up again after bottom dead center.

Catalytic Detoxication of Coal Combustion Gases (연탄 연소가스의 촉매제독에 관한 연구)

  • Tuwon Chang;Young Sun Uh;Youn Soo Sohn
    • Journal of the Korean Chemical Society
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    • v.29 no.6
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    • pp.656-663
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    • 1985
  • A catalytic conversion of carbon monoxide has been studied in a coal combustion system. Three different catalysts were prepared by impregnating 0.2% platinum on three different types of supports, ${\gamma}-Al_2O_3$ pellets, ceramic foam and honeycomb. These catalysts have shown an excellent initial activities in the coal combustion system, but they were rapidly deactivated in repeated uses. Among these catalysts ceramic foam has shown to be better than others both in activities and durabilities. The main cause of deactivation seems to be ascribed to poisoning by zinc metal and sulfur compounds and to decrease in platinum surface area by sintering.

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A Study of in vitro Scavenging Reactions of Acrylamide with Glutathione Using Electrospray Ionization Tandem Mass Spectrometry

  • Cui, Sheng-Yun;Kim, Seung-Jin;Jo, Sung-Chan;Lee, Yong-Moon;Lee, Yong-Ill
    • Bulletin of the Korean Chemical Society
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    • v.26 no.8
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    • pp.1235-1240
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    • 2005
  • A combination of electrospray ionization and tandem mass spectrometry was used to characterize the scavenging reactions of acrylamide (AA) in the presence of glutathione (GSH) in vitro. In the presence of GSH, AA was deactivated effectively and scavenged by reactions consuming small amount of GSH. Reaction products and structural information were identified using collision-induced dissociation (CID) in an ion trap mass spectrometer. In the mixture of GSH and AA, significant increase in abundance of fragment ion peak was observed at m/z 233, which was identified as $[Cys-Glu]^+$, formed by the elimination of glycine moiety of GSH. GSH also contributes to the AA scavenging reaction by conjugating with AA through the sulfhydryl group in cysteine moiety. The probable scavenging reaction pathway of AA in the presence of GSH has been proposed based on the CID experimental data.

Breakage Detection of Small-Diameter Tap Using Vision System in High-Speed Tapping Machine with Open Architecture Controller

  • Lee, Don-Jin;Kim, Sun-Ho;Ahn, Jung-Hwan
    • Journal of Mechanical Science and Technology
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    • v.18 no.7
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    • pp.1055-1061
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    • 2004
  • In this research, a vision system for detecting breakages of small-diameter taps, which are rarely detected by the indirect in-process monitoring methods such as acoustic emission, cutting torque and motor current, was developed. Two HMI (Human Machine Interface) programs to embed the developed vision system into a Siemens open architecture controller, 840D, were developed. They are placed in sub-windows of the main window of the 840D and can be activated or deactivated either by a softkey on the operating panel or the M code in the NC part program. In the event that any type of tool breakage is detected, the HMI program issues a command for an automatic tool change or sends an alarm signal to the NC kernel. An evaluation test in a high-speed tapping machine showed that the developed vision system was successful in detecting breakages of small-diameter taps up to M1.

Accumulation of the Carbonaceous Species on the Ni/Al2O3 Catalyst during CO2 Reforming of Methane

  • Lee, Jae-Hee;Joo, Oh-Shim;Baek, Young-Soon;Yu, Yong-Ho;Jung, Kwang-Deog
    • Bulletin of the Korean Chemical Society
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    • v.24 no.11
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    • pp.1623-1626
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    • 2003
  • The dependency of the rate of $CO_2$ reforming of methane on the catalyst loading and the reactor size was examined at a fixed temperature of $750\;^{\circ}C$ and a fixed GHSV of 18000 mL(STP)/$g_{cat}.h$. The conversion of methane in $CO_2$reforming decreased with increase in the reactor size. The catalyst was severely deactivated with increase in the catalyst amount. The amount of carbonaceous species combustible below $550\;^{\circ}C$, determined by TPO experiments with the used catalyst samples increased with increase in the catalyst amount, which was again confirmed by XRD and TEM experiments. The increase of the carbonaceous species combustible below $550\;^{\circ}C$ may be due to the suppression of the reverse Boudouard reaction, since the $CO_2$ reforming of methane, a highly endothermic reaction, resulted in lowering the reaction temperature.

Regenerability of a Ni catalyst in the catalytic steam reforming of biomass pyrolysis volatiles

  • Arregi, Aitor;Lopez, Gartzen;Amutio, Maider;Barbarias, Itsaso;Santamaria, Laura;Bilbao, Javier;Olazar, Martin
    • Journal of Industrial and Engineering Chemistry
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    • v.68
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    • pp.69-78
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    • 2018
  • A study has been carried out of the regenerability of a commercial Ni catalyst used in the steam reforming of the volatiles from biomass pyrolysis (gases and bio-oil), determining the evolution of the reaction indices (conversion, product yields and $H_2$ production) in successive reaction-regeneration cycles. The causes of catalyst deactivation (coke deposition and Ni sintering) have been ascertained characterizing the deactivated and regenerated catalysts by TPO, TEM, TPR and XRD. Catalyst activity is not fully recovered by coke combustion in the first cycles due to the irreversible deactivation by Ni sintering, but the catalyst reaches a pseudo-stable state beyond the fourth cycle, reproducing its behaviour in subsequent cycles.

Sonochemical Reaction of Fullerene Oxides, [C70(O)n](n≥1) with Aromatic Amines (방향족 아민 화합물과 풀러렌 산화물의 [C70(O)n](n≥1)의 초음파 화학 반응)

  • Ko, Weon-Bae;Park, Byoung-Eun;Lee, Young-Min
    • Elastomers and Composites
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    • v.43 no.1
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    • pp.31-38
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    • 2008
  • Sonochemical reaction of fullerene oxides, $[C_{70}(O)_n](n\geq1)$ with several aromatic amines such as 4-nitroaniline, 3-nitroaniline, and 4-isopropylaniline, in the presence of $FeCl_3$ were investigated under ultrasonic irradiation. This method is applicable to a wide variety of aromatic amines especially ring deactivated, to afford the corresponding cleavage products under mild conditions. The aminated fullerenes were confirmed by MALDI-TOF-MS and UV-vis spectra.

Design of Context-Aware-Based Drone Control Mechanism by Using Two-Factor (이중요소를 이용한 상황인지 기반 드론 제어 메커니즘 설계)

  • Oh, Yoon-Seok;Kim, Aeyoung;Seo, Seung-Hyun
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.29 no.1
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    • pp.57-66
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    • 2019
  • Drones, which are used in various fields, are vulnerable to various security threats such as physical deodorization attacks and information leakage attacks because they operate in an unmanned environment and use wireless communication with weak security. In particular, research is needed to prevent damages such as leakage of stored information and unauthorized use due to illegal drone deodorization. In this paper, we propose a context - aware drone control mechanism that protects stored internal information and prevents unauthorized use when the drones are illegally deactivated. We also demonstrated the feasibility of the proposed mechanism as a prototype implementation and experiment.