• 한국가축번식학회지
• /
• 제22권3호
• /
• pp.265-276
• /
• 1998

In an attempt to evaluate the function of MAP kinase of porcine oocytes and to develop a method of assessment for kinase activity, we used MBP as a substrate to detect the MAP kinase activity of porcine oocytes matured in in vitro. The MAP kinase which had lower activity during the first 20 hours of culture started to show an increased amount of activity at 25 hours at which a collapse in nuclear membrane was induced. Significant (P<0.05) a, pp.ared at 30 hours of being cultured. The gel phosphorylation method, MBP which has been known to be a substrate for kinase such as cdc2 kinase, was phosphorylated at two positions corresponding to ERK 1 (44kDa) and ERK2 (42 kDa) which are known as mammalian MAP kinase. The existence of MARKK and MAP kinase were identified with western blotting at 0 hour culture of immature GV oocytes. The amount of those proteins did not increase during 40 hours of culture, which suggest that the increase of MAP kinase activity was caused by phosphorylaton rather than due to change in protein amount. MAPKK and MAP kinase were shown to be dephosporylated with deactivated at M 1 stage by inhibition of protein synthesis with cycloheximide added at the strat following the cultrue. We have reulsts that indicate the existedence of MAP kinase cascade which was activated simultaneously with start of porcine oocyte maturation (GVBD).

• 한국염색가공학회지
• /
• 제25권4호
• /
• pp.271-278
• /
• 2013

In order to investigate the effect of gallotannin treatment to ceramide-containing fabrics on atopic skin diseases, the agglomeration of standard protein BSA and the deactivation of model enzyme were examined. The gallotannin treated on ceramide-containing fabrics precipitated the standard protein, BSA, and therefore deactivated the model enzyme by 70% at 6% treatment concentration. Wash durability should be improved after around 5 cycles of washing. Clinical test of the gallotannin-treated fabrics was carried out on mice for two test items, transepidermal water loss assay and severity score of diseased skin of mice. The results showed significant level of improvement of atopic skin diseases compared with the negative controled.

• 한국자동차공학회논문집
• /
• 제16권6호
• /
• pp.148-156
• /
• 2008

This paper is the first of 2 companion papers which investigate in-cylinder swirl generation characteristics according to inlet valve angle. Two DOHC 4 valve engines, one has wide intake valve angle and the other has narrow valve angle, were used to compare the characteristics of swirl motion generation in the cylinder. One intake port was deactivated to induce swirl flow. A PIV (Particle Image Velocimetry) was applied to measure in-cylinder velocity field according to inlet valve angle during intake stroke. The results show that the stronger swirl motion is observed in wide valve angle engine at the early intake stage; however, the swirl motion is gradually distorted by the intake flow component passing through valve area near the cylinder wall as the stroke proceeds. The tumble motion also does so in wide angle. On the contrary, the swirl and tumble motions, which are not clear at the initial stage, become better and better arranged as the piston goes down and up again after bottom dead center.

• 대한화학회지
• /
• 제29권6호
• /
• pp.656-663
• /
• 1985

연탄 연소시 발생하는 일산화탄소의 촉매전환에 의한 제독 실험을 실시하였다. 구형 ${\gamma}-Al_2O_3$ pellets, ceramic foam, honeycomb 등 세가지 다른 형태의 지지체에 0.2%의 백금을 담지시킨 후 촉매를 연탄연소 실험에 응용한 결과 첫회 사용시에는 일산화탄소의 제거효과가 모두 우수하였으나 반복 사용할 경우 사용횟수에 따라 촉매활성 전하 현상이 뚜렷하게 나타났다. 사용한 촉매양에 비하여 ceramic foam 촉매가 제독효과 및 내구성에서 가장 우수하였다. 촉매활성저하 원인은 아연 및 황화합물에 의한 촉매독이 뚜렷하였고 소결 등에 의한 백금 활성면적의 감소현상도 동시에 나타났다.

• Bulletin of the Korean Chemical Society
• /
• 제26권8호
• /
• pp.1235-1240
• /
• 2005

A combination of electrospray ionization and tandem mass spectrometry was used to characterize the scavenging reactions of acrylamide (AA) in the presence of glutathione (GSH) in vitro. In the presence of GSH, AA was deactivated effectively and scavenged by reactions consuming small amount of GSH. Reaction products and structural information were identified using collision-induced dissociation (CID) in an ion trap mass spectrometer. In the mixture of GSH and AA, significant increase in abundance of fragment ion peak was observed at m/z 233, which was identified as $[Cys-Glu]^+$, formed by the elimination of glycine moiety of GSH. GSH also contributes to the AA scavenging reaction by conjugating with AA through the sulfhydryl group in cysteine moiety. The probable scavenging reaction pathway of AA in the presence of GSH has been proposed based on the CID experimental data.

• Journal of Mechanical Science and Technology
• /
• 제18권7호
• /
• pp.1055-1061
• /
• 2004

In this research, a vision system for detecting breakages of small-diameter taps, which are rarely detected by the indirect in-process monitoring methods such as acoustic emission, cutting torque and motor current, was developed. Two HMI (Human Machine Interface) programs to embed the developed vision system into a Siemens open architecture controller, 840D, were developed. They are placed in sub-windows of the main window of the 840D and can be activated or deactivated either by a softkey on the operating panel or the M code in the NC part program. In the event that any type of tool breakage is detected, the HMI program issues a command for an automatic tool change or sends an alarm signal to the NC kernel. An evaluation test in a high-speed tapping machine showed that the developed vision system was successful in detecting breakages of small-diameter taps up to M1.

• Bulletin of the Korean Chemical Society
• /
• 제24권11호
• /
• pp.1623-1626
• /
• 2003

The dependency of the rate of $CO_2$ reforming of methane on the catalyst loading and the reactor size was examined at a fixed temperature of $750\;^{\circ}C$ and a fixed GHSV of 18000 mL(STP)/$g_{cat}.h$. The conversion of methane in $CO_2$reforming decreased with increase in the reactor size. The catalyst was severely deactivated with increase in the catalyst amount. The amount of carbonaceous species combustible below $550\;^{\circ}C$, determined by TPO experiments with the used catalyst samples increased with increase in the catalyst amount, which was again confirmed by XRD and TEM experiments. The increase of the carbonaceous species combustible below $550\;^{\circ}C$ may be due to the suppression of the reverse Boudouard reaction, since the $CO_2$ reforming of methane, a highly endothermic reaction, resulted in lowering the reaction temperature.

• Journal of Industrial and Engineering Chemistry
• /
• 제68권
• /
• pp.69-78
• /
• 2018

A study has been carried out of the regenerability of a commercial Ni catalyst used in the steam reforming of the volatiles from biomass pyrolysis (gases and bio-oil), determining the evolution of the reaction indices (conversion, product yields and $H_2$ production) in successive reaction-regeneration cycles. The causes of catalyst deactivation (coke deposition and Ni sintering) have been ascertained characterizing the deactivated and regenerated catalysts by TPO, TEM, TPR and XRD. Catalyst activity is not fully recovered by coke combustion in the first cycles due to the irreversible deactivation by Ni sintering, but the catalyst reaches a pseudo-stable state beyond the fourth cycle, reproducing its behaviour in subsequent cycles.

• Elastomers and Composites
• /
• 제43권1호
• /
• pp.31-38
• /
• 2008

[ $FeCl_3$ ]를 첨가한 초음파 조건에서 4-nitroaniline, 3-nitroaniline, and 4-isopropylaniline등의 방향족 아민 화합물과 풀러렌 산화물$[C_{70}(O)_n](n\geq1)$을 반응시켰다. 이 반응은 방향족 아민 화합물을 사용한 풀러렌 산화물 쪼개짐 반응이다. MALDI-TOF-MS and UV-vis spectra를 사용하여 생성된 화합물이 아민화 풀러렌 유도체임을 확인하였다.

• 정보보호학회논문지
• /
• 제29권1호
• /
• pp.57-66
• /
• 2019

최근 다방면으로 활용되고 있는 드론은 무인 환경에서 동작하고 보안이 취약한 무선 통신을 사용하기 때문에 물리적 탈취 공격, 정보 유출 공격 등 다양한 보안 위협에 취약하다. 특히, 불법 드론 탈취로 인해 저장정보 유출 및 무단 도용 등의 피해를 예방하기 위한 연구가 필요하다. 이에 본 논문에서는 드론이 불법적으로 탈취당한 경우에, 저장된 내부 정보를 보호하고, 무단 도용을 예방할 수 있는 상황인지 기반 드론 제어 메커니즘을 제안하고, 제안 메커니즘의 실현성을 프로토타입 구현 및 실험으로 보였다.