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Response of Ovaries and Cyst According to Treatment with Dinoprost or Fenprostalene in Dairy Cows with Ovarian Luteal Cyst (황체낭종우에서 Dinoprost 또는 Fenprostalene 치료에 대한 난소 및 낭종의 반응)

  • 강현구;김일화;백인석;손창호;이청산
    • Journal of Veterinary Clinics
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    • v.21 no.2
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    • pp.154-160
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    • 2004
  • This study was carried out to monitor the response of ovaries and cyst according to treatment with dinoprost or fenprostalene in dairy cows with ovarian luteal cyst. Twenty cows were diagnosed as luteal cysts by rectal palpation, ultrasonography and progesterone analysis. The cystic cows were treated with dinoprost or fenprostalene. All the animals were re-examined by ultrasonography and blood was collected for the measurement of plasma progesterone concentration at day 0 (the day of treatment), 3, 13 and 24, respectively. Mean plasma progesterone concentrations on day -11 and day -1 before treatment were 2.1$\pm$0.2 ng/ml and 2.8$\pm$0.3 ng/ml, respectively. On day 3 and day 13 were 0.3$\pm$0.1 ng/ml and 4.3$\pm$0.2 ng/ml, respectively. Mean cystic wall thickness on day -11 and day -1 were 3.2$\pm$0.2 mm and 3.9$\pm$0.2 mm, respectively. And on day 3 was 2.4$\pm$0.3 mm. The responses of luteal cyst after treatment noted during ultrasonography included dramatical degeneration of the luteal tissue of cystic wall on day 3 (all cows), slowly reduction of cyst size (cyst resolution) until last examination (8 cows), complete disappearance on day 13 (7 cows) and no changes of cyst size (5 cows). A group of 10 cows with luteal cysts injected fenprostalene compared with another 10 cows treated dinoprost showed a slightly higher pregnancy rate on first service after initial treatment (50.0 vs 30.0%). But treatment effects of dinoprost or fenprostalene did not significantly different results for each groups. This study suggested that the response of the cyst according to treatment revealed various types. Therefore, veterinarians should have attention on monitoring of the response of cystic ovaries after treatment, specially on no change of cyst size after treatment.

Calculation of the Dispersion Coefficient by the Dissolution Experiment of DNAPL Pool (DNAPL Pool의 용해실험에 의한 분산계수 산정)

  • 정경영;배열호;최상일
    • Journal of Korea Soil Environment Society
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    • v.1 no.1
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    • pp.19-27
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    • 1996
  • Nonaqueous phase liquids(NAPL) not readily dissolved in water exist as a separate fluid phase. Groundwater contamination by NAPL such as organic solvents and petroleum hydrocarbons becomes major public concerns because of their long-term persistence in the subsurFace and their ability to contaminate large volumes of wate. Dense.-than-water NAPL(DNAPL) spilled into the subsurface penetrate through the saturated zone and ultimately form DNAPL pools on the bottom of the aquifer. The dissolution of DNAPL from these pools depends on the molecular diffusion coefficient, the vertical dispersivity, the groundwater velocity, the solubility, and the pool length. In this study, the vertical transverse dispersion coefficients for simulating the dissolution of DNAPL from such pools were obtained from the dissolution experiment. Under the experimental conditions used, the vertical transverse dispersion coefficients calculated were 1.86$cm^2$/day, 2.90$cm^2$/day and 4.51$cm^2$/4ay for seepage velocities of 59.2cm/day, 94.3cm/day and 158.0cm/day, respectively. And the vertical transverse dispersivity was 0.03024cm.

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EFFECTS OF THE SINGLE AND FRACTIONATED IRRADIATION ON THE MICROVASCULATURE OF THE RAT SUBMANDIBULAR GLAND (단일 및 분할 방사선조사가 백서 악하선 미세혈관계에 미치는 영향)

  • Kim Seok-Ho;Choi Karp-Shik
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.23 no.1
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    • pp.71-85
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    • 1993
  • The purpose of the study was to investigate the effects of the single and fractionated irradiation on the microvascular structure of the submandibular gland in rats. For this study, 90 Sprague-Dawley strain rats were irradiated to their neck region with equal split doses of 9Gy for a 4 hours interval and 15Gy single dose by 6MV X-irradiation and sacrificed on the 1st, 3rd, 7th, 14th and 27th day after irradiation. The author observed histological changes at Hematoxylin and Eosin staining and PAS staining under a light microscope, and also observed distribution and structural changes of the microvasculature in rat submandibular gland using a scanning electron microscope by forming vascular resin casting. The results were as follows: 1. In the light microscopic examination, the microvasculature was slightly dilated and decreased in number on the 1st day after irradiation, and increase in number of microvasculature was observed on the 3rd day after irradiation. And then distribution of microvasculature was markedly increased on the 7th day after iradiation, but decreased on th 14th day after irradiation again. Such changes were greater in the single irradiated group than in the fractionated irradiated group. 2. The reaction to PAS staining on glandular cell was decreased on the 1st and the 3rd day after irradiation, and recovered on the 7th day after irradiation. The reaction was decreased on the 14th day after irradiation again, and recovered on the 28th day after irradiation. Changes were more apparent in the single irradiated group. 3. In the scanning electron microscopic examination, early changes of microvasculature were decreased capillary density, dilation of conduits and meandering. Increased capillary dentsity or anastomosis due to vascular reproduction and smooth curved running were observed on the 7th and 14th day after irradiation. Decreased capillary and smooth running tendency were observed on the 28th day after irradiation again. Such changes were greater in the single irradiated group than in the fractionated irradiated group.

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A Study on Electrophoretic Pattern and Immunosuppressive Activity of Uterine Secretory Protein in Early Pregnant Sows (초기 임신돈에 있어서 자궁분비단백질의 전기영동상과 면역억제력에 관한 연구)

  • 김경순;정영채;김창근;한기영;김형태;윤종택;김종대;최선호
    • Korean Journal of Animal Reproduction
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    • v.13 no.3
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    • pp.179-187
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    • 1989
  • The experiment was carried out to study the profile of uterine specific protein during early pregnancy in sows and to test it's immunosuppressive activity. Uterine protein samples were obtained by flushing the uterine horn on Day 3, 6, 9, 12 and 15 of the estrous cycle and the pregnancy respectively and the protein concentration of each sample was determined. The change of uterine protein was examined by sodium dodecyl sulfate(SDS)-PAGE. The immunosuppressive activity of uterine secretory protein was investigated according to the lymphocyte blastogenesis response to mitogen. The results of this experiment are summarized as follows ; 1. The uterine protein during estrous cycle and early pregnancy was relatively constant up to Day 9, but increased on Day 12. Maxium total protein values were found on Day 15. The concentration of serum proteins were about 82-95 mg during estrous cycle, but decreased to about 70-82 mg during early pregnancy. 2. The proteins components similar electrophoretic patterns(PAGE) that were no differences (band ; a, b, c, d, e, f, g, I) on Days 3, 6 and 9 of the estrous cycle and pregnancy. But there were more 2 bands specifically on Day 12 of the pregnancy and on Day 15 of estrous cycle and showed more 4 bands on Day 15 of early pregnancy. They seemed to be acidoprotein and their average molecular weight were 38,000, 22,300 and 12,600. 3. When uterine protein were added 500$\mu\textrm{g}$/ml, there was no immunosuppresive activity on Day 3 of estrous cycle and lymphocyte blastogenesis was slightly suppressed on Day 3 of pregnancy. The immunosuppressive activity on Day 9 of estrous cycle and pregnancy appeared in 500$\mu\textrm{g}$/ml and 150$\mu\textrm{g}$/ml, respectively the uterine protein on Day 12 and 15 showed immunosuppresive activity, which at the level of 150$\mu\textrm{g}$/ml during non-pregnancy and at the level of 100 to 125$\mu\textrm{g}$/ml during early pregnancy, respectively.

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Study on the Changes in Limb Circumferences and Muscle Areas of Critically Ill Patients using Ventilators (인공호흡기(ventilator)를 사용하는 중환자의 사지둘레와 사지근육면적 변화에 관한 연구)

  • Hwang, Young-Hui;Choe, Myoung-Ae
    • Journal of Korean Academy of Nursing
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    • v.38 no.6
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    • pp.874-880
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    • 2008
  • Purpose: The purpose of this study was to identify muscle atrophy in critically ill patients on ventilators. A comparison was made between limb circumferences and muscle areas on the day of admission to an intensive care unit (ICU) and on the 8th day after admission. Methods: The data were collected from 30 critically ill patients using ventilators at S hospital ICU in Seoul during the period from October 2005 to April 2006. Limb circumferences and skinfold thickness were measured on the day of admission and on the 8th day after admission to the ICU. Limb circumferences and skinfold thickness were measured on the right mid-arm, right mid-thigh, and right mid-calf using a measuring tape and a skinfold calipers. Limb muscle areas were calculated by an equation after measuring limb circumferences and skinfold thickness. The data were analyzed by paired t-test and independent t-test. Results: 1) Mid-arm circumference, mid-thigh circumference and mid-calf circumference on the 8th day after admission to the ICU were significantly less than those on the day of admission to the ICU, 2) Mid-arm muscle area, mid-thigh muscle area and mid-calf muscle area on the 8th day after admission to the ICU were significantly less than those on the day of admission to the ICU, 3) Steroid and vecuronium medication didn't affect changes in limb circumferences (mid-arm, mid-thigh, mid-calf) and muscle areas (mid-arm, mid-thigh, mid-calf on the 8th day after admission to the ICU compared to the day of admission. Conclusion: Limb muscle atrophy may occur on the 8th day after admission to the ICU in critically ill patients using ventilator.

Characteristics of Interior Space and Planning for Elderly Day Care Center in Korea (우리나라 노인주간보호시설의 실내공간 특성과 계획안)

  • 이선주;권오정
    • Journal of Families and Better Life
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    • v.21 no.1
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    • pp.1-12
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    • 2003
  • Day care center for the elderly is an alternative for community care for frail elderly who need social participation and physical cares and services outside the family. The purpose of this study was to identify the present situation of day care center for the elderly and to suggest desirable interior space plans for elderly day care center. Data were collected by two ways; a mail survey and case studies of existing day care centers. Five cases of day care centers were analyzed to find out Physical and space problems, available programs and services. Also, the staffs in 96 day care centers for the elderly in nationwide were selected for a mail survey and 68.8 percentage of all was collected and analyzed. From the result of case studies, narrow space and lack of accessibility features for wheelchair or other prosthetic devices were the major problems. From the result of a mail survey analysis, the elderly users were over 73 years old, and high proportion had suffered from dementia and apoplexy Also, staffing ration was 1:7 In other words, one staff was caring of 7 old people, and supporting staff was insufficient. Therefore, supportive, therapeutic environment Was more important issue to improve quality of care. Separate program room and outdoor space, lounging space, treatment room by gardening, separate counselling room, bedroom, a simple kitchen unit, staff office, separate physical treatment room, resting room for staff was indicated more necessarily. When priority of planning interior space was safety followed by accessability and convenience, it was also reported that sire of each room was generally narrow. The most serious problem in interior space was the lack and narrowness of space, and accessible features for mobility. Based on the literature review and the findings of this study, two types of floor plans for elderly day care center were suggested;‘a mixed type for the elderly with and without dementia’and‘a separate type for the elderly with dementia.’The main goals for designing interior space for two types of elderly day care center were to provide safe environment, encouraging environment for remaining abilities of users, sanitary environment, therapeutic environment, familiar environment, and socially-encouraging environment. The recommendations for further studies were suggested.

Effects of Repetitive Transcranial Magnetic Stimulation on Enhancement of Cognitive Function in Focal Ischemic Stroke Rat Model (국소 허혈성 뇌졸중 모델 흰쥐의 인지기능에 반복경두개자기자극이 미치는 효과)

  • Lee, Jung-In;Kim, Gye-Yeop;Nam, Ki-Won;Lee, Dong-Woo;Kim, Ki-Do;Kim, Kyung-Yoon
    • Journal of the Korean Society of Physical Medicine
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    • v.7 no.1
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    • pp.11-20
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    • 2012
  • Purpose : This study is intended to examine the repetitive transcranial magnetic stimulation on cognitive function in the focal ischemic stroke rat model. Methods : This study selected 30 Sprague-Dawley rats of 8 weeks. The groups were divided into two groups and assigned 15 rats to each group. Control group: Non-treatment after injured by focal ischemic stroke; Experimental group: application of repetitive transcranial magnetic stimulation(0.1 Tesla, 25 Hz, 20 min/time, 2 times/day, 5 days/2 week) after injured by focal ischemic stroke. To assess the effect of rTMS, the passive avoidance test, spatial learning and memory ability test were analyzed at the pre, 1 day, $7^{th}$ day, $14^{th}$ day and immunohistochemistric response of BDNF were analyzed in the hippocampal dentate gyrus at $7^{th}$ day, $14^{th}$ day. Results : In passive avoidance test, the outcome of experimental group was different significantly than the control group at the $7^{th}$ day, $14^{th}$ day. In spatial learning and memory ability test, the outcome of experimental group was different significantly than the control group at the $7^{th}$ day, $14^{th}$ day. In immunohistochemistric response of BDNF in the hippocampal dentate gyrus, experimental groups was more increased than control group. Conclusion : These result suggest that improved cognitive function by repetitive transcranial magnetic stimulation after focal ischemic stroke is associated with dynamically altered expression of BDNF in hippocampal dentate gyrus and that is related with synaptic plasticity.

Groundwater Balance in Urban Area (도시지역의 지하수수지)

  • Lee, Seung-Hyun;Bae, Sang-Keun
    • Journal of Environmental Science International
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    • v.20 no.12
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    • pp.1553-1560
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    • 2011
  • The study analyzes groundwater balance with regard to the water recharge and discharge which contain urbanization components in Suyeong-gu, Busan. It also verifies the reliability and accuracy improvement on the analysis of the balance. The result of the study is viewed as preliminary data which are useful to develop, utilize and manage groundwater. The average quantity of groundwater recharge is 6,014.1 $m^3$/day in the research area during the last ten year period(from 1998 to 2007). The outflow from drainage areas to rivers and coasts is 149.3 $m^3$/day, the inflow from rivers and coasts to drainage area is 439.9 $m^3$/day. The use of the water is 4,243.0 $m^3$/day. The outflow caused by subway in line No.2 and No.3 through Suyeong-gu and the one by building an underground electric complex is 1,500.0 $m^3$/day. The leakage of water works is 6514.9 $m^3$/day. The inflow and outflow of sewerage is 5082.2 $m^3$/day from groundwater to sewer. The amount of groundwater recharge, the inflow from rivers and coasts to drainage area, and the leakage of water works belong to the amount of groundwater inflow and the total amount is 12,968.9 $m^3$/day. The amount of outflow from drainage area to rivers and coasts, the use of groundwater, outflow by subway and underground electric complex tunnel and the amount of inflow of the water to sewerage belong to the amount of outflow of groundwater and the sum amount is 13,031.5 $m^3$/day. The gap between the amount of inflow and outflow of groundwater is 62.6 $m^3$/day, which is considered to reflect the trend that the short term drop in the amount of rainfall results in the amount of groundwater recharge and that the amount of outflow from drainage area to rivers and coasts decreases.

Estimation of Gestational Age by Measurement of Fetal and Extra-fetal Structures in Miniature Schnauzer Bitches

  • Kim, Bang-Sil;Son, Chang-Ho
    • Journal of Veterinary Clinics
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    • v.25 no.6
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    • pp.501-505
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    • 2008
  • Serial ultrasonographic examinations were performed on 9 Miniature Schnauzer bitches from day 15 of gestation until parturition to determine the ultrasonographic measurement of gestational structures. Ovulation was designated the day that plasma progesterone concentration exceeded 4.0 ng/ml (day 0). Extra-fetal structures were measured from day 17 or 18 to 60. Outer uterine diameter (OUD) increased from $6.50{\pm}1.06mm$ ($Mean{\pm}SD$) to $50.89{\pm}5.62mm$, inner chorionic cavity diameter (ICCD) increased from $2.10{\pm}0.15mm$ to $37.15{\pm}4.36mm$, and length of placenta (PL) increased from $7.50 {\pm}1.41mm$ to $40.62{\pm}3.27mm$. OUD and ICCD were significantly and linearly relative to gestational age especially through day 37, whereas PL was not significantly relative to gestational age. Of the extra-fetal structures, ICCD was the most accurate for estimation of gestational age before day 38 of gestation. Fetal structures increased at a linear rate to parturition. Fetal crown-rump length (CRL) increased from $2.55{\pm}0.07mm$ on day 22 to $85.25{\pm}9.89mm$ on day 48. Fetal head diameter (HD) increased from $3.43{\pm}0.64mm$ on day 27 to $25.06{\pm}0.41mm$ on day 63. Fetal body diameter (BD) increased from $ 5.96{\pm}0.84mm$ on day 30 to $43.76{\pm}3.36mm$ on day 63. Of the fetal structures, HD was the most accurate for estimation of gestational age after day 38 to parturition.

The Experimental Model on Morphological Changes of the Liver Cell Treated with Ethanol (에타놀 처치 간세포(肝細胞)의 형태학적 변화에 대한 실험모델)

  • Lee, Jae-Hyun
    • Korean Journal of Veterinary Research
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    • v.23 no.1
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    • pp.45-56
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    • 1983
  • In order to prepare an experimental model on the basis of morphological changes of liver, 90 rabbits were allocated into four groups and 5, 15, 30 and 50% of ethanol (5ml/day) were dosed to each group for 5, 10, 20, 30, 60 and 90 days, and observed the livers with light and electron microscope. The results obtained are summarized as follows: 1. Light microscopically, the hydropic changes were found in the liver from 10th day after 5% and 15% ethanol ingestion, and these findings were remarkable as time goes by. Necrosis of hepatocytes, however, was most remarkable on 60th and 90th day after ingestion. The proliferation of interlobular connective tissue was found from 30th day in this group. 2. The hydropic necrosis of liver cells was found from 5th day in 30% and 50% ethanol dosing groups. The proliferation of interlobular connective tissues and infiltration of the lymphocytes were observed from 30th day in 30% ethanol ingestion group, however, it was found from 20th day in 50% ethanol ingestion group. 3. Electron microscopically, the appearance of various lipid droplets and dilated rER, slightly increased sER and glycogen field, dense mitochondria with scanty cristae and hydropic change were observed on 5th day after 5% ethanol ingestion. These findings were more remarkable on 30th and 90th day. Especially, disorganized outer and inner membrane of mitochondria and autophagic vacuoles were appeared on 90th day. The similar findings were also found in 15%, 30% and 50% ethanol ingestion groups, however, the appearance of fibrillar and myelin-like structures were observed from 30th day after 15% ethanol ingestion. The severe hydropic change and increased sER were observed on 90th day after 15% ethanol ingestion. From 5th day after 30% and 50% ethanol ingestion these findings were more remarkable.

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