• 한국군사과학기술학회지
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• 제27권4호
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• pp.507-515
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• 2024

Microbial forensics is a scientific discipline for analyzing evidence related to biological crimes by identifying the origin of microorganisms. Multiple locus variable number tandem repeat analysis(MLVA) is one of the microbiological analysis methods used to specify subtypes within a species based on the number of tandem repeat in the genome, and advances in next generation sequencing(NGS) technology have enabled in silico anlysis of full-length whole genome sequences. In this paper, we analyzed unknown samples provided by Robert Koch Institute(RKI) through The United Nations Secretary-General's Mechanism(UNSGM)'s external quality assessment exercise(EQAE) project, which we officially participated in 2023. We confirmed that the 3 unknown samples were B. anthracis through nucleic acid isolation and genetic sequence analysis studies. MLVA results on 32 loci of B. anthracis were analysed by using genome sequences obtained from NGS(NextSeq and MinION) and Sanger sequencing. The MLVA typing using short-reads based NGS platform(NextSeq) showed a high probability of causing assembly error when a size of the tandem repeats was grater than 200 bp, while long-reads based NGS platform(MinION) showed higher accuracy than NextSeq, although insertion and deletion was observed. We also showed hybrid assembly can correct most indel error caused by MinION. Based on the MLVA results, genetic identification was performed compared to the 2,975 published MLVA databases of B. anthracis, and MLVA results of 10 strains were identical with 3 unkonwn samples. As a result of whole genome alignment of the 10 strains and 3 unknown samples, all samples were identified as B. anthracis strain A4564 which is associated with injectional anthrax isolates in heroin users.

• 한국도서관정보학회지
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• 제54권4호
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• pp.307-334
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• 2023

본 연구의 목적은 2023년 미국도서관협회(American Library Association, ALA)인가 문헌정보학 학위과정에서 제공하는 데이터 사서와 관련된 교과목의 강의계획서의 내용을 분석하는 것이다. 2023년 ALA인가 문헌정보학과에서 공개한 강의계획서 184개를 수집하였다. 데이터 사서의 주요 역량인 데이터 관리와 큐레이션, 데이터 분석 및 시각화, 메타데이터, 정보 서비스, 도서관 경영, 연구 방법론, 학술 도서관, 컴퓨터 프로그래밍, 데이터베이스 과목 등을 식별하였다. 이에 기반하여 데이터 사서와 관련된 강의계획서 127개를 식별한 후, 강의계획서의 내용을 집중 분석하였다. 구체적으로 교과목 명, 교과목 목표, 교과목 설명, 교육 내용, 과제(assignments)를 집중 분석하였다. 데이터 사서 양성을 위한 국내외 교과목의 비교를 위하여 국외 61개 문헌정보학과에서 개설한 교과목 명 3,045개, 교과목 설명 2,559개와 국내 37개 문헌정보학과에서 개설한 1,330개의 교과목 명을 수집 및 분석하였다. 국내외 문헌정보학과 모두 데이터 사서 양성을 위한 교과목을 골고루 개설하였음을 확인하였다. 본 연구의 결과는 향후 문헌정보학과에서 데이터 사서 양성을 위한 교육 과정의 개발 및 개정 시 논의의 시작점이 될 수 있는 기초 자료로 활용되기를 기대한다.

• Korean Journal of Radiology
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• 제23권5호
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• pp.529-538
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• 2022

Objective: Since its introduction in 2011, the CT/MRI diagnostic Liver Imaging Reporting and Data System (LI-RADS) has been updated in 2014, 2017, and 2018. We evaluated the impact of CT/MRI diagnostic LI-RADS on liver MRI research methodology for the diagnosis of hepatocellular carcinoma (HCC). Materials and Methods: The MEDLINE, EMBASE, and Cochrane databases were searched for original articles reporting the diagnostic performance of liver MRI for HCC between 2011 and 2019. The MRI techniques, image analysis methods, and diagnostic criteria for HCC used in each study were investigated. The studies were classified into three groups according to the year of publication (2011-2013, 2014-2016, and 2017-2019). We compared the percentage of studies adopting MRI techniques recommended by LI-RADS, image analysis methods in accordance with the lexicon defined in LI-RADS, and diagnostic criteria endorsed by LI-RADS. We compared the pooled sensitivity and specificity between studies that used the LI-RADS and those that did not. Results: This systematic review included 179 studies. The percentages of studies using imaging techniques recommended by LI-RADS were 77.8% for 2011-2013, 85.7% for 2014-2016, and 84.2% for 2017-2019, with no significant difference (p = 0.951). After the introduction of LI-RADS, the percentages of studies following the LI-RADS lexicon were 0.0%, 18.4%, and 56.6% in the respective periods (p < 0.001), while the percentages of studies using the LI-RADS diagnostic imaging criteria were 0.0%, 22.9%, and 60.7%, respectively (p < 0.001). Studies that did not use the LI-RADS and those that used the LIRADS version 2018 showed no significant difference in sensitivity and specificity (86.3% vs. 77.7%, p = 0.102 and 91.4% vs. 89.9%, p = 0.770, respectively), with some difference in heterogeneity (I² = 94.3% vs. 86.7% in sensitivity and I² = 86.6% vs. 53.2% in specificity). Conclusion: LI-RADS imparted significant changes in the image analysis methods and diagnostic criteria used in liver MRI research for the diagnosis of HCC.

• 대한한의학회지
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• 제45권1호
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• pp.17-43
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• 2024

Objectives: Underpinned by the context of a Korean traditional medicine cohort study on healthy aging, this research primarily aims to guide the selection of Clinical Outcome Assessments (COAs) for elderly healthy aging patient registry research, offering insights into the selection process; and secondly, to streamline the resource-intensive process of obtaining permissions for validated COAs, benefiting future traditional Korean medicine clinical researchers. Methods : In this study, we identified outcomes through a review of previous studies, followed by a process involving expert consultations to select the final outcomes. Subsequently, for the selected outcomes that were Clinical Outcome Assessments (COAs) developed tools, we searched in commercial databases to confirm the availability of Korean versions and the necessity of obtaining permissions. Finally, we obtained permissions for their utilization and, when needed, acquired the original instrument questionnaire through payment. Results: Through a literature review of existing observational studies, a total of 57 outcomes were selected, with 19 of them identified as COA instruments. Upon verifying usage permissions for these 19 instruments, it was found that 17 required author-specific permissions, and among these, 2 needed a purchase as they were commercially available. Conclusion: This study provides a detailed overview of outcome selection and permission acquisition for elderly patient registry research. It underscores the importance of Clinical Outcome Assessment (COA) tools and the rigorous approval process, aiming to enhance research reliability. Continuous verification of COA information is essential, and future research should explore Core Outcome Set (COS) development through consensus-building approaches like Delphi studies.

• International Journal of Stem Cells
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• 제16권2호
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• pp.215-233
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• 2023

Background and Objectives: MYC, also known as an oncogenic reprogramming factor, is a multifunctional transcription factor that maintains induced pluripotent stem cells (iPSCs). Although MYC is frequently upregulated in various cancers and is correlated with a poor prognosis, MYC is downregulated and correlated with a good prognosis in lung adenocarcinoma. MYC and two other MYC family genes, MYCN and MYCL, have similar structures and could contribute to tumorigenic conversion both in vitro and in vivo. Methods and Results: We systematically investigated whether MYC family genes act as prognostic factors in various human cancers. We first evaluated alterations in the expression of MYC family genes in various cancers using the Oncomine and The Cancer Genome Atlas (TCGA) database and their mutation and copy number alterations using the TCGA database with cBioPortal. Then, we investigated the association between the expression of MYC family genes and the prognosis of cancer patients using various prognosis databases. Multivariate analysis also confirmed that co-expression of MYC/MYCL/MYCN was significantly associated with the prognosis of lung, gastric, liver, and breast cancers. Conclusions: Taken together, our results demonstrate that the MYC family can function not only as an oncogene but also as a tumor suppressor gene in various cancers, which could be used to develop a novel approach to cancer treatment.

• 한국학교ㆍ지역보건교육학회지
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• 제24권4호
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• pp.39-50
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• 2023

Objectives: This study aimed to provide a scoping review of the impact of COVID-19 on people with disabilities. Methods: PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) Group's key questions (PICO) for systematic reviews, a literature search was conducted from January 1, 2020 to June 30, 2023 using the "AND" search formula. The databases used were Korea DB: RISS, SCIENCEON, and KoreaMed, and International DB: PubMed, CINAHL, and Embase. Results: Among a total of 22 selected articles, 4 studies were published in korea journals: 3 were survey studies (75.0%), 1 was an analysis study (25.0%), and 18 studies were published in international journals. Through a literature review, we confirmed the differences in difficulties between PwD (People with Disability) and PwoD (People without Disability) in the COVID-19 situation, differences by disability type, and post-COVID-19 impact. In order to examine this systematically and objectively, the results were examined by dividing them, which are components based on the International Classification of Functioning, Disability and Health (ICF). Conclusion: In order to respond well to crises and establish effective health policies in similar pandemic situations in the future, understanding and exploration of more diverse types and areas of disability are necessary. In addition, the development and application of appropriate health education and health promotion programs for disability type and ICF component area are required. In addition, it is necessary to develop and apply appropriate health education and health promotion programs tailored to the type of disability and ICF component area.

• 문화기술의 융합
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• 제10권2호
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• pp.215-224
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• 2024

본 연구는 국내 간호대학생의 신생아 시뮬레이션 실습교육 관련 연구동향을 살펴보기 위한 서술적 조사연구이다. 문헌수집은 국내 6개 전자 데이터베이스에서 ('신생아 시뮬레이션')과 ('간호학생' OR '간호대학생' OR '학생간호사')로 조합하여 검색한 결과 총 17편을 선정하였다. 연구결과는 2011년~2015년 7편으로 시작하여 2016년~2020년 5편과 2021년~2023년 5편으로 다소 감소하는 것으로 나타났다. 연구설계는 양적연구 총 16편(94%)으로 중재연구 15편(88%), 서술적 조사연구 1편(6%)이고, 혼합연구는 1편(6%)이었다. 시뮬레이션 주요 사례 주제는 고위험 신생아 간호 9편(52%), 호흡곤란증후군 중환아 간호 3편(18%), 신생아 간호 3편(18%) 정상 신생아 간호 1편(6%), 신생아 응급 기도 간호 1편(6%)으로 나타났다. 주요 결과 변수 및 적용효과는 임상수행능력 5편(19.2%)이 가장 많았고 그 다음 순으로 실습만족도 3편(11.5%)이었고 적용 효과가 있는 것으로 나타났다. 결론적으로 간호대학생의 신생아 시뮬레이션 실습교육의 확대, 반복연구, 질적연구 등 다양한 연구방법이 요구된다.

• 한국비블리아학회지
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• 제35권1호
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• pp.169-192
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• 2024

본 연구는 지명 전거데이터의 체계적 구축과 표준화 필요성에 따라 지명 전거데이터 기술에 필요한 한국 특성에 맞는 지침을 개발함으로써 국가 전거 고품질화의 기반을 제공하고자 하는 목적으로 수행되었다. 이를 위해 지명전거와 관련된 국내·외 동향 및 사례 조사를 실시하고, 지명 전거 구축을 위한 각국의 규칙과 지침을 분석했다. 이러한 현황 조사 및 규칙 분석을 바탕으로 지명 전거데이터 구축에 필요한 개념 범위와 용어를 정의하고 지명 전거데이터 지침 개발의 방향성을 설정하였다. 또한, 분석 결과, 지침이 포함해야 할 범위, 체계, 기존 규칙의 참조 내용과 방식 등을 결정하였다. 본 연구에서 제안하는 지침의 체계는 Original RDA와 NCR을 기준으로 삼아 설정하였다. 분석 과정에서 도출된 시사점을 중심으로 구축 대상 범위, 우선 지명 선정 및 기록, 이형 지명의 기록, 지명의 속성 등으로 지침을 구성하고 내용을 제시하여 한국 실정에 맞는 지명 전거데이터 기술 지침(안)을 제안하고자 하였으며, 이에 따른 향후 논의사항을 밝혔다.

• 한방재활의학과학회지
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• 제34권1호
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• pp.65-81
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• 2024

Objectives This study aimed to understand the general research trends, applicated disease, and methodology of transcutaneous/percutaneous vagus nerve stimulation, contemplating its clinical use in traditional Korean medicine and future research directions. Methods A scoping review was conducted following Arksey and O'Malley Framework Stage and adhering to the PRISMA extension for scoping reviews: checklist and explanation. Papers published until October 30, 2023, were investigated across 10 databases (PubMed, Embase, Scopus, Web of Science, China National Knowledge Infrastructure, Oriental Medicine Advanced Searching Integrated System, Korean Studies Information Service System, KMbase, Science ON, Research Information Sharing Service. The search terms used were 'Transcutaneous/Percutaneous vagus nerve stimulation'. Results Since 2021, the application of transcutaneous/percutaneous vagus nerve stimulation for musculoskeletal symptoms has been actively researched, predominantly in Asia (37%), Europe (37%), and North America (21%). All 19 papers were part of clinical studies. Chronic pain was noted that most applied disease, it also was found to potentially aid in acute post-surgical pain relief. Major assessment tools include not only simple pain metrics but also pain perception, vagal nerve tension, quality of life, and inflammatory markers. Most procedures were carried out through the ear, which offers a favorable site for therapeutic stimulation without notable side effects. And parameter analysis, frequencies typically ranged around 25 Hz to 30 Hz, while pulse widths were commonly set at 250 ㎲ or 300 ㎲. Conclusions Transcutaneous/percutaneous vagus nerve stimulation is easily accessible through acupuncture in Korean medicine. Therefore, if future studies establish parameters and clinical significance, it could be utilized as a therapeutic modality.

• 대한약침학회지
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• 제27권1호
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• pp.14-20
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• 2024

Objectives: Frozen shoulder (FS) is one of the most challenging shoulder disorders for patients and clinicians. Its symptoms mainly include any combination of stiffness, nocturnal pain, and limitation of active and passive glenohumeral joint movement. Conventional treatment options for FS are physical therapy, nonsteroidal anti-inflammatory drugs, injection therapy, and arthroscopic capsular release, but adverse and limited effects continue to present problems. As a result, pharmacoacupuncture (PA) is getting attention as an alternative therapy for patients with FS. PA is a new form of acupuncture treatment in traditional Korean medicine (TKM) that is mainly used for musculoskeletal diseases. It has similarity and specificity compared to corticosteroid injection and hydrodilatation, making it a potential alternative injection therapy for FS. However, no systematic reviews investigating the utilization of PA for FS have been published. Therefore, this review aims to standardize the clinical use of PA for FS and validate its therapeutic effect. Methods: The protocol was registered in Prospero (CRD42023445708) on 18 July 2023. Until Aug. 31, 2023, seven electronic databases will be searched for randomized controlled trials of PA for FS. Authors will be contacted, and manual searches will also be performed. Two reviewers will independently screen and collect data from retrieved articles according to predefined criteria. The primary outcome will be pain intensity, and secondary outcomes will be effective rate, Constant-Murley Score, Shoulder Pain and Disability Index, range of motion, quality of life, and adverse events. Bias and quality of the included trials will be assessed using the Cochrane handbook's risk-of-bias tool for randomized trials. Meta analyses will be conducted using Review Manager V.5.3 software. GRADE will be used to evaluate the level of evidence for each outcome. Results: This systematic review and meta-analysis will be conducted following PRISMA statement. The results will be published in a peer-reviewed journal. Conclusion: This review will provide scientific evidence to support health insurance policy as well as the standardization of PA in clinical practice.