• Journal of Crop Science and Biotechnology
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• v.10 no.3
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• pp.175-184
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• 2007

Two subspecies, japonica and indica, have been reported in rice, which differ in several ecotypic traits. However, reproductive barriers in hybrid progenies between subspecies have been major obstacles in breeding programs using inter-subspecific hybridization. As the first step to elucidate the reproductive barriers, we developed subspecies-specific(SS) STS markers in this study. A total of 765 STS primers were designed through comparing DNA sequences at every $2{\sim}3$cM interval between japonica and indica rices, which are available at Web DBs such as IRGSP, NCBI, TIGR, and GRAMENE, and tested for subspecies-specificity using 15 indica and 15 japonica varieties of diverse origin. Of them, 67 STS markers were identified as SS STS markers and their subspecies-specificity scores were estimated. The SS markers were dispersed throughout the genome along chromosomes. Of them, 64 SS markers were mapped on an RIL population derived from a Dasanbyeo(indica)/TR22183(japonica) cross. Genomic inclination of RILs was evaluated based on the genotyping with different types of markers. Association test between markers and segregation distortion revealed that segregation distortion might not be the cause of generating SS markers. The SS markers will be applicable to estimate the genomic inclination of varieties or lines and to study the differentiation of indica and japonica, and ultimately to breed true hybrid rice varieties in which desirable characters from both subspecies are recombined.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.2
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• pp.127-131
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• 2002

Critical nitrogen concentration (Nc), which is defined as the minimum % N in shoots required to maintain the maximum growth rate of top dry weight (W) at any time, was determined for rice plant. Using two rice varietal groups, japonica varieties and an indica $\times$ japonica "Dasanbyeo", 18 data points fulfilling the statistical criteria for determining Nc were obtained through eight N-fertilization experiments over two years at Suwon (37$^{\circ}$16'N), Korea. Nc dilution curve for each variety was obtained by fitting the Nc-W relationship to power function. However, The critical nitrogen curves for the two variety groups were not different statistically. Thus, a Nc dilution curve was fitted for the Nc data points pooled over the two variety groups and proposed in rice as: Nc=4.08, where W＜1.73 t h $a^{-1}$ , Nc=5.197 $W^{0.425}$3/ ($R^2$=0.964), where 1.73 t h $a^{-1}$ ＜W＜12 t h $a^{-l}$. The Nc for W＜1.73 t h $a^{-l}$ were estimated as a constant value of 4.08%, the mean value of the maximum N concentration for N-limiting condition and the minimum N concentration for N non-limiting condition. The model for Nc is applicable to diagnosing the nitrogen nutrition status during the rice growth period from emergence to heading stage. The Nc curve well discriminated the 144 data points between the N limiting and the N non-limiting groups regardless of varieties, cultural methods, and years.-limiting groups regardless of varieties, cultural methods, and years.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.1
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• pp.41-47
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• 2006

To understand the heading habit of recently developed 20 Korean rice cultivars, rice plants in a phytotron were exposed to different temperature: $22.5^{\circ}C(day\;27^{\circ}C/night\;18^{\circ}C),\;27.5^{\circ}C(day\;32^{\circ}C/night\;23^{\circ}C)$, and day-length conditions: 10, 12, 13, 14, 15 hours. Four rice cultivars (Sobibyeo, Juanbyeo, Ilpumbyeo and Shindongjinbyeo) showed relatively short Basic Vegetative Phase (BVP) of 17 to 18 days, while Dasanbyeo showed the longest (35 days) BVP, compared to other remaining 15 tested cultivars which exhibited 24 to 31 days of BVP. In this experiment, it was tried out to separate the eliminable vegetative phase into photosensitivity and thermo-sensitivity with two different pathways. Many tested cultivars, however, exhibited quite different responses under low temperature and / or long day-length conditions. Especially, Surabyeo and Juanbyeo were the most difficult cultivars to separate into photo- or thermo-sensitivity in that the eliminable vegetative phase of these two cultivars increased greatly only under low temperature $(22.5^{\circ}C)$ and long day-length (15 hr.) conditions. Regarding the heading response to temperatures, tested cultivars could be categorized into 2 groups. In 1st group of rice cultivars, the eliminable vegetative phase decreased almost equally as the temperature changed from $20.0^{\circ}C\rightarrow22.5^{\circ}C\rightarrow25.0^{\circ}C\rightarrow27.5^{\circ}C$. In contrast, the 2nd group of rice cultivars exhibited eliminable vegetative phase slowly decreasing when the temperature changed from $22.5^{\circ}C\rightarrow25.0^{\circ}C\rightarrow27.5^{\circ}C$, but rapidly decreasing when the mean temperature changed from $20.0^{\circ}C\;to\;22.5^{\circ}C$. All the cultivars belonged to 2nd group, the heading date would be very delayed if cool summer comes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.4
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• pp.434-439
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• 2008

The amount of salt to make seed sorting solution of the specific gravity of 1.13 was reconsidered and determined as 3.8 kg salt in 18 L water, which is lower amount than currently used. Five rice cultivars were examined. Percent germination and seedling emergence were not similar. Seedling emergence rate of Japonica varieties, Nampyungbyeo and Daerypbyeo-1 were 87% and 95% under specific gravity of 1.13, respectively. Seedling emergence rate of Tongil type variety, Dasanbyeo was as high as 67% in specific gravity of 1.06. Seedling emergence rate of waxy rice, Hwasunchalbyeo and Aranghangchalbyeo were examined. Seedling emergence rate was 94% in both cultivars in specific gravity of 1.04. Seedling emergence rate was same in specific gravity of 1.08 which is generally used for selecting seed currently. Early growth (plant height, leaf number, and dry weight) were not significantly different by specific gravity within species. In all cultivars except waxy rice, highest seedling emergence rate was observed in specific gravity of 1.13 which is currently used for selection and decreased as specific gravity is lowed. However, considering total amount of seeds in each group of specific gravity, amount of seed in lower specific gravity group is relatively small and total seedling emergence rate within variety dose not show big difference. However, if seeds with low speicific gravity are produced due to the bad grain filling condition and consequently total seed content of low specific gravity increases, results will be differnt. Reduction in total growth and yield could occur. It will be important to comply with the seed sorting criterion of 1.13 for Japonica, 1.06 for Tongil, and 1.04 for waxy rice variety to ensure the maximum rice growth and yield.

• Plant Breeding and Biotechnology
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• v.5 no.4
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• pp.371-389
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• 2017

Supplying sufficient rice to growing populations is a global challenge. Hybrid indica rice varieties exploiting heterosis have increased yields, but inter-subspecific crosses between indica and japonica varieties are hampered by sterility. Examination and genetic understanding of yield heterosis in indica/japonica crosses addressing yield barriers are basic requirements. In this study, QTLs for heterosis of yield traits were identified in indica-japonica recombinant inbred lines (RILs) using a total of 178 RILs originating from Dasanbyeo (indica) ${\times}$ TR22183 (japonica) (DT-RILs) and their backcrossed populations. Nine of sixty-six major quantitative trait loci (QTLs) identified in DT-RILs exhibited heterosis. Heterosis QTLs clustered with other traits on chromosomes 1, 4, and 8, and clusters were conserved between different RILs. The clusters contained several known yield enhancement genes/QTLs. Specific heterotic allele combinations contributed to four major heterosis QTLs, particularly for panicle and spikelet number traits. Heterosis for yield and yield-related traits was explained by the harmonized effects of overdominance, dominance, and epistatic interactions in inter-subspecific breeding populations.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.68 no.3
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• pp.99-105
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• 2023

Starch characteristics and pasting properties of Tongil-type rice varieties with different amylose content were analyzed. Three different Tongil-type rice varieties and one Japonica-type rice variety were examined for their properties. Tongil-type rice varieties have longer panicles and higher rice yield (721-765 kg per 10a) than Boramchan, a Japonica-type high-yield rice variety. The protein content of the Tongil-type rice variety was higher than that of Boramchan. Japonica-type rice varieties had lower amylose content than Japonica-type rice varieties, except for Amimyeon (Milyang355). Amimyeon had higher protein content than the other varieties, and its amylose content was particularly high at 39.2%. The distribution of starch granule sizes of the four varieties was similar, and the particle size corresponding to D50 was approximately 87.8-81.9 ㎛. The pasting properties of rice flour varied among varieties. The Dasanbyeo and Hanarum2 amylogram patterns were similar. These two varieties had a higher peak viscosity (PV) and lower setback (SB) than Boramchan. In the case of Amimyeon, the hot paste viscosity (HPV), cool paste viscosity (CPV), and SB were significantly higher than those of the other Toingil-type varieties, and the breakdown (BD) was very low, showing pasting properties significantly different from that of the other varieties. The onset (T_o), peak (T_p), and conclusion temperature (Tc) of gelatinization of rice flour from Amimyeon were lower than those of the other tested varieties. In addition, the gelatinization temperature, measured using differnetial scanning calorimetry (DSC), and BD, measured using al rapid visco analyzer (RVA) were low in Amimyeon. Amylose content showed a high positive correlation with pasting time (PTi), HPV, and SB and a negative correlation with PV and BD.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.463-467
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• 2004

This study was carried out to estimate of leaf area index (LAI) rapidly using plant canopy analyzer, comparing with specific leaf area (SLA) and leaf area ratio (LAR) in rice from 2001 to 2002 at Honam Agricultural Research Institute in Iksan Korea. The relationship between LAI values taken by plant canopy analyzer and by leaf area meter showed high correlation at each growth stages of rice. LAIs obtained by plant canopy analyzer were highly correlated with that by leaf area meter which were the highest in Dongjinbyeo and the lowest in Hapcheon1. Specific leaf area (SLA) of all rice cultivars were tend to decrease remarkably with the progress of growth stage. It was found that the SLA of Dasanbyeo was the highest and that of Hapcheon1 was the lowest among rice cultivars tested. Leaf area ratios (LARs) was also decreased with the progress of growth stage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.516-521
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• 2004

The straw of thirty one rice varieties were evaluated for their feed value and related agronomic traits. The rice straw were hand-harvested, dried to constant weight at $75^{\circ}C$ and ground through a 20 mesh seive in a Wiley mill, analyzed with crude protein (CP), acid detergent fiber (ADF) and neutral detergent fiber (NDF). Relative feed value (RFV) was calculated from NDF and ADF. The sum of standardized score was estimated by dry weight of rice straw, content of CP, ADF and NDF. The straw yield of Daeanbyeo was 725.9 kg/10a, showed heighest value among the varieties and remainder was in the order of Keumnambyeo, Donginbyeo #1 and Chucheongbyeo. Crude protein (CP) content in a Dasanbyeo was higher than those in other varieties. The content of ADF in a Junghwabyeo and NDF in a Sobaegbyeo were $34.3\%$ and $63.8\%$, respectively, showed lowest value among the varieties. The rice straw of Dunnaebyeo, Obongbyeo, Seoanbyeo, Keumobyeo, Hwaseongbyeo, Noganbyeo and Gyehwabyeo belonged to the high feed value varieties by estimation of cluster analysis, sum of standardized score and RFV. The content of CP was found to be positively related with dry weight of leaf and grain, but negatively related with heading days after seeding, culm length, specific leaf weight (SLW) and dry weight of stem. ADF and NDF were found to be positively related with heading days after seeding, culm length, SLW and dry weight of leaf, but negatively related with dry weight of stem. The sum of standardized score and RFV were the only positive relationship with dry weight of stem and negative relationship with other traits.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.3
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• pp.221-228
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• 2001

This study was carried out to investigate the effect of germination condition and drying temperature on growth and physicochemical properties of brown rice. Three brown rice seeds of Ilpumbyeo, Dasanbyeo and Heugjinjubyeo were stored at room temperature for six weeks to test the time-sequence germination viability. Relatively stable germination ratio was maintained until 2 weeks after storage. However, 3 weeks after storage, germination ratio of brown rice seeds started to decrease rapidly and their germination ratio was lower than 80%. For this reason, brown rice was recommended for seeding within 2 weeks after hulling. During the initial 5 days, germination ratio of 24 hours pre-soaking brown rice was higher about 2-3% than that of non-soaking brown rice. The $25^{\circ}C$ was considered as the most favorable temperature for brown rice germination, because of the high germination ratio and desirable coleoptile growth of the brown rice, and little seed rotting symptoms. The scanning electron micrographs showed the structural differences between hot-air dried and freeze dried germinated-brown rice kernel. In the freeze dried germinated-brown rice, seed coat (pericarp, tegmen and aleurone layer) was mechanically disrupted from the endosperm, and many cleavages were observed among starch storing cells and starch granules. The endosperm of freeze-dried brown rice kernels formed the sponge-like structures and showed the fragile traits. For this reason, hot-air drying is considered as more suitable method than freeze drying for germinated-brown rice. The crude protein and amylose contents were slightly changed, but there were no significant differences during the germination period. Crude fiber content was decreased, but crude Int and total amino acid contents were increased as seeding days increased. A rapid increase in $\alpha$-amylase activities of germinating brown rice was observed at S days after seeding, and $\alpha$-amylase activities were decreased from 8 days after seeding. Total free sugar contents were decreased during the germination period. There was continuous decline in the contents of sucrose and glucose until 8 days after seeding, but fructose and maltose content were gradually increased from the 5 days after seeding.

• Molecules and Cells
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• v.25 no.3
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• pp.417-427
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• 2008

Comparison of maps and QTLs between populations may provide us with a better understanding of molecular maps and the inheritance of traits. We developed and used two reciprocal $BC_1F_1$ populations, IP/DS//IP and IP/DS//DS, for QTL analysis. DS (Dasanbyeo) is a Korean tongil-type cultivar (derived from an indica x japonica cross and similar to indica in its genetic make-up) and IP (Ilpumbyeo) is a Korean japonica cultivar. We constructed two molecular linkage maps corresponding to each backcross population using 196 markers for each map. The length of each chromosome was longer in the IP/DS//IP population than in the IP/DS//DS population, indicating that more recombinants were produced in the IP/DS//IP population. Distorted segregation was observed for 44 and 19 marker loci for the IP/DS//IP and IP/DS//DS populations, respectively; these were mostly skewed in favor of the indica alleles. A total of 36 main effect QTLs (M-QTLs) and 15 digenic epistatic interactions (E-QTLs) were detected for the seven traits investigated. The phenotypic variation explained (PVE) by M-QTLs ranged from 3.4% to 88.2%. Total PVE of the M-QTLs for each trait was significantly higher than that of the E-QTLs. The total number of M-QTLs identified in the IP/DS//IP population was higher than in the IP/DS//DS population. However, the total PVE by the M-QTLs and E-QTLs together for each trait was similar in the two populations, suggesting that the two $BC_1F_1$ populations are equally useful for QTL analysis. Maps and QTLs in the two populations were compared. Eleven new QTLs were identified for SN, SF, GL, and GW in this study, and they will be valuable in marker-assisted selection, particularly for improving grain traits in tongil-type varieties.