• Food Science and Biotechnology
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• 제16권5호
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• pp.858-862
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• 2007

The present study was conducted to examine the antioxidant activities and neuroprotective effects of methanolic extracts from Schizonepeta tenuifolia Briquet (STE). STE ($100\;{\mu}g/mL$) showed $43.33\;{\mu}M$ of total phenolic content, 64.43% of radical scavenging activity, and 0.157 of reducing power. In addition, the effect of STE on $H_2O_2$-induced DNA damage in human leucocytes was evaluated by the comet assay, where STE was a dose dependent inhibitor of DNA damage induced by $200{\mu}M$ of $H_2O_2$. The protective effect of STE against $H_2O_2$-induced oxidative damage on PC12 cells was investigated by an 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) reduction assay and lactate dehydrogenase (LDH) release assays. After 2 hr of cell exposure to $H_2O_2\;(500\;{\mu}M)$, a marked reduction in cell survival was observed. However, this reduction was significantly prevented by $1-50\;{\mu}g/mL$ of STE. Therefore, these results suggest that STE could be a new antioxidant candidate against neuronal diseases.

• BMB Reports
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• 제47권10호
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• pp.575-580
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• 2014

In this study, we investigate whether arsenite-induced DNA damage leads to p53-dependent premature senescence using human glioblastoma cells with p53-wild type (U87MG-neo) and p53 deficient (U87MG-E6). A dose dependent relationship between arsenite and reduced cell growth is demonstrated, as well as induced ${\gamma}H2AX$ foci formation in both U87MG-neo and U87MG-E6 cells at low concentrations of arsenite. Senescence was induced by arsenite with senescence-associated ${\beta}$-galactosidase staining. Dimethyl- and trimethyl-lysine 9 of histone H3 (H3DMK9 and H3TMK9) foci formation was accompanied by p21 accumulation only in U87MG-neo but not in U87MG-E6 cells. This suggests that arsenite induces premature senescence as a result of DNA damage with heterochromatin forming through a p53/p21 dependent pathway. p21 and p53 siRNA consistently decreased H3TMK9 foci formation in U87M G-neo but not in U87MG-E6 cells after arsenite treatment. Taken together, arsenite reduces cell growth independently of p53 and induces premature senescence via p53/p21-dependent pathway following DNA damage.

• 한국태양에너지학회 논문집
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• 제38권2호
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• pp.1-13
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• 2018

This paper deals with the NREL (National Renewable Energy Laboratory) 5-MW reference wind turbine. The controller which include MPPT (Maximum power point tracking) control algorithm and tower load reduction control algorithm was designed by MATLAB Simulink. This paper propose a tower damper algorithm to improve the existing tower damper algorithm. To improve the existing tower damper algorithm, proposed tower damper algorithm were applied the thrust sensitivity scheduling and PI control method. The thrust sensitivity scheduling was calculated by thrust force formula which include thrust coefficient table. Power and Tower root moment DEL (Damage Equivalent Load) was set as a performance index to verify the load reduction algorithm. The simulation were performed 600 seconds under the wind conditions of the NTM (Normal Turbulence Model), TI (Turbulence Intensity)16% and 12~25m/s average wind speed. The effect of the proposed tower damper algorithm is confirmed through PSD (Power Spectral Density). The proposed tower damper algorithm reduces the fore-aft moment DEL of the tower up to 6% than the existing tower damper algorithm.

• Food Science and Biotechnology
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• 제17권2호
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• pp.343-348
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• 2008

In this study, antioxidant activity of methanol extract of Glycyrrhiza uralensis Fisch (GUE) against $H_2O_2$-induced DNA damage in human leucocytcs and cell death in PC12 cells was determined. The effect of GUE on $H_2O_2$-induced DNA damage in human leucocytcs was evaluated by the comet assay, where GUE ($1-50\;{\mu}g/mL$) was a dose dependent inhibitor of DNA damage induced by $H_2O_2$. The protective effect of GUE against $H_2O_2$-induced damage on PC12 cells was investigated by MTT reduction assay and lactate dehydrogenase release assay. A marked reduction in cell survival induced by $H_2O_2$ was significantly prevented by $1-50\;{\mu}g/mL$ of GUE. The enzyme activity of caspase-3 was elevated in $H_2O_2$-treated PC12 cells, while preincubation with GUE for 30 min inhibited $H_2O_2$-induced caspase-3 activation in a dose-dependent manner. In conclusion, GUE ameliorates $H_2O_2$-induced DNA damage in human leucocytes and has neuroprotective effect by preventing cell death in PC12 cell, suggesting that GU may be a potential candidate for novel therapeutic agents for neuronal diseases associated with oxidative stress.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2000년도 추계학술대회논문집A
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• pp.250-255
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• 2000

Damage induced by low velocity impact loading in aircraft composite laminates is the form of failure which is occurred frequently in aircraft. Low velocity impact can be caused either by maintenance accidents with tool drops or by in-flight impacts with debris. As the consequences of impact loading in composite laminates, matrix cracking, delamination and eventually fiber breakage for higher impact energies can be occurred. Even when no visible impact damage is observed, damage can exist inside of composite laminates and the carrying load of the composite laminates is considerably reduced. The reduction of strength and stiffness by impact loading occurs in compressive loading due to laminate buckling in the delaminated areas. The objective of this study is to determine inside damage of composite laminates by impact loading and to determine residual compressive strength and the damage growth mechanisms of impacted composite laminates. For this purpose a series of impact and compression after impact tests are carried out on composite laminates made of carbon fiber reinforced epoxy resin matrix with lay up pattern of $[({\pm}45)(0/90)_2]s$ and $[({\pm}45)(0)_3(90)(0)_3({\pm}45)]$. UT-C scan is used to determine impact damage characteristics and CAI(Compression After Impact) tests are carried out to evaluate quantitatively reduction of compressive strength by impact loading.

• 한국지반공학회논문집
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• 제35권1호
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• pp.43-53
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• 2019

본 논문에서는 하수관거 파손에 따른 토사 유실로 인해 발생되는 지중 공동 및 이완영역의 규모를 정량적으로 분석하기 위해 하수관거 파손 폭과 하수관거 상부의 토피고 변화를 고려한 유한요소 수치해석을 실시하였다. 수치해석에서는 선행 연구에서 검증된 역학모델을 적용하였으며, 강제변위법을 이용하여 토사 유실에 따른 하수관거 파손부 주변지반의 역학적 거동을 모사하였다. 수치해석 결과로부터 파손 폭 및 토피고 변화에 따른 모형지반의 간극비 분포, 지표면 침하, 전단응력 분포 특성을 분석하였다. 또한, 지중의 전단응력 감소 특성을 분석하여 지중 공동 및 이완영역의 경계를 결정하였으며, 파손 폭과 토피고 변화에 따른 공동 및 이완영역의 발생 규모를 정량적으로 평가하였다.

• Smart Structures and Systems
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• 제18권6호
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• pp.1233-1250
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• 2016

Cracks in plate-like structures are some of the main reasons for destruction of the entire structure. In this study, a novel two-stage methodology is proposed for damage detection of flexural plates using an optimized artificial neural network. In the first stage, location of damages in plates is investigated using curvature-moment and curvature-moment derivative concepts. After detecting the damaged areas, the equations for damage severity detection are solved via Bat Algorithm (BA). In the second stage, in order to efficiently reduce the computational cost of model updating during the optimization process of damage severity detection, multiple damage location assurance criterion index based on the frequency change vector of structures are evaluated using properly trained cascade feed-forward neural network (CFNN) as a surrogate model. In order to achieve the most generalized neural network as a surrogate model, its structure is optimized using binary version of BA. To validate this proposed solution method, two examples are presented. The results indicate that after determining the damage location based on curvature-moment derivative concept, the proposed solution method for damage severity detection leads to significant reduction of computational time compared with direct finite element method. Furthermore, integrating BA with the efficient approximation mechanism of finite element model, maintains the acceptable accuracy of damage severity detection.

• Structural Monitoring and Maintenance
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• 제5권2호
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• pp.243-260
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• 2018

Detection of damages in fibre reinforced plastic (FRP) composite structures is important from the safety and serviceability point of view. Usually, damage is realized as a local reduction of stiffness and if dynamic responses of the structure are sensitive enough to such changes in stiffness, then a well posed inverse problem can provide an efficient solution to the damage detection problem. Usually, such inverse problems are solved within the framework of pattern recognition. Support Vector Machine (SVM) Algorithm is one such methodology, which minimizes the weighted differences between the experimentally observed dynamic responses and those computed using the finite element model- by optimizing appropriately chosen parameters, such as stiffness. A damage detection strategy is hereby proposed using SVM which perform stepwise by first locating and then determining the severity of the damage. The SVM algorithm uses simulations of only a limited number of damage scenarios and trains the algorithm in such a way so as to detect damages at unknown locations by recognizing the pattern of changes in dynamic responses. A rectangular fiber reinforced plastic composite plate has been investigated both numerically and experimentally to observe the efficiency of the SVM algorithm for damage detection. Experimentally determined modal responses, such as natural frequencies and mode shapes are used as observable parameters. The results are encouraging since a high percentage of damage cases have been successfully determined using the proposed algorithm.

• 한국군사과학기술학회지
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• 제13권6호
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• pp.1051-1057
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• 2010

Database for the damage reference by armor piercing bullet test was established for both tube and plate specimens having a range of thickness. As the inclined angles of hit are increasing, it has been found that penetration damage diameter tends to increases accordingly in both specimen of the tube and plate, and such penetration damage diameter on the rear side becomes bigger than those on the front side. The tube specimen showed that the damage becomes bigger when central areas rather than the peripheral were hit. Through the plate test, it also has been found that the penetration ballistic limit for Al alloy is about 25.4mm and that of stainless steel about 12.7mm. From the fatigue analysis results using the database for damage reference, it has been identified whether the safety requirements of military aircraft could be met.

• 한국안전학회지
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• 제36권5호
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• pp.27-35
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• 2021

This study investigated the change in stiffness and deflection of a scaffold working plate caused by damage to a cross-beam in the plate. Experiments were conducted considering various load and damage conditions of cross-beams. A cross-beam falling off from the working plate was considered damaged. The load-displacement curves of specimens at the center of the uniform load showed that the working plate stiffness decreased by 14.66%-1.89%, depending on the load interval due to the damage of one cross-beam. A reduction in the stiffness of 33.94%-40.76% resulted from the damage of two cross-beams. Moreover, the displacement increased by an average of 25% when one cross-beam was damaged and an average of 65% when two cross-beams were damaged. Therefore, damage to the cross-beam in the working plate can potentially cause accidents and harm workers. As the load increases, the risk of an accident due to the aforementioned damage also increases because the stiffness remarkably decreases with the load increase. Further, the damage to the cross-beam mainly reduces the stiffness but increases the displacement rather than the strength of the working scaffold plate.