• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1612-1612
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• 2001

The production of grain for export and domestic use is one of Australia's most important agricultural industries, and the NIR technique has been used extensively over many years for the routine monitoring of grain quality, particularly moisture and protein content. Because most Australian grain is intended for human food production, the determinants of grain quality for livestock feed, apart from protein, have been largely ignored. However the increasing use of grain for feeding to pigs, poultry, beef cattle and dairy cows has led to an important national research project entitled “Premium Grains for Livestock”. Two of the objectives of this project are to determine the compositional and functional characteristics of grains which influence their nutritional quality for the various classes of livestock, and to adopt rapid and objective analytical tests for these quality criteria. NIR has been used in this project firstly to identify a set of grain samples from a large population of breeders' lines which showed a wide spectral variation, and hence a potentially wide variation in nutritional value. The selected samples were not only subjected to an extensive array of chemical, physical and in vitro analyses, but also were grown out to produce sufficient quantities of grain to feed to animals in vivo studies. Additional grains were also strategically selected from farms in order to include the effect of weather damage, such as rain, drought and frost. In this study to date, NIR calibrations have been derived or attempted, on both ground and whole grains, for in vivo dry matter digestibility (DMD), pepsin-cellulase dry matter disappearance, protein, fat, acid detergent fibre, neutral detergent fibre, starch, in sacco DMD and in vitro assays to simulate starch digestion in the lumen and small intestine. Results so far indicate high calibration accuracy for chemical components (SECV 0.3 to 2.6%) and very promising statistics for in vivo DMD (SECV 1.8, $R^2$ 0.93, SD 7.0, range 61.9 to 92.3, n=60). There appears to be some potential for NIR to estimate some in vitro properties, depending upon the accuracy of reference methods and appropriate sample populations. Current work is in progress to extend the range of grains with in vivo DMD values (a very laborious and expensive process) and to increase the robustness of the various NIR calibrations, with the aim of implementing uniform testing procedures for nutritional value of grains throughout Australia.

• 한국동물생명공학회지
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• 제38권4호
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• pp.189-198
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• 2023

Background: Brassica oleracea var. italica (broccoli), a rich source of antioxidants, can prevent various diseases and improve human health. In this study, we investigated the antioxidative effects of broccoli sprout extract on oxidative stress induced by lipopolysaccharide and cisplatin in cell and organ tissue models. Methods: Antioxidative effect of BSE was evaluated using DPPH and ABTS in RAW 364.7 cells, and effects of BSE on testes were investigated using Cisplatin-induced testicular damage model with an in vitro organ culture system. Results: The DPPH assay showed that the antioxidant activity of the alcoholic broccoli sprout extract was higher than that of the water extract. Additionally, the expression levels of antioxidation-related genes, Nrf2, Gsr, HO-1, and catalase, were significantly increased in broccoli sprout extract-treated RAW 264.7 cells, and the extract suppressed lipopolysaccharide-induced mitochondrial dysfunction. Based on the results in the RAW 264.7 cell culture, the antioxidative effects of the extracts were investigated in a mouse testis fragment culture. The expression of Nrf2, HO-1, and Ddx4 was clearly decreased in cisplatin-treated mouse testis fragments and not in both broccoli sprout extract- and cisplatin-treated mouse testis fragments. In addition, the oxidative marker O-HdG was strongly detected in cisplatin-treated mouse testis fragments, and these signals were reduced by broccoli sprout extract treatment. Conclusions: The results of this study show that broccoli sprout extracts could serve as potential nutraceutical agents as they possess antioxidant effects in the testes.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2014년도 춘계학술대회
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• pp.295-298
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• 2014

2000년 이후 한국에서 기후변화(아열대성 및 잦은 이상기온) 및 농축산물 수입개방에 따른 국내 고병원성AI, 구제역, 가축질병 5회 발생으로 3조8천억원 피해와 기유입 확산되는 재선충의 10여년간 공적 방제비 2천383억원이 투입되었다. 또한 유입 가능성이 높은 흰개미 등의 외래식물해충으로 국가의 경제 사회적 피해와 영향이 증가되는 실정이다. 본 논문에서는 FTA확대에 따른 농식물로 인한 국가경제피해에 대한 대응 가능한 농업 농촌분야에 사물(IoT) 기반의 국가재난형 동 식물위성조기경보체계 운용전략에 대한 연구와 정책 수립을 연구한다. 또한 국가적 재난의 피해발생과 책임소재에 대한 정부의 피해보상 및 법 제도적인 책임 수행의 사전 증거자료 수집과 사후 보상 근거로서의 포렌식 기술이 포함된 정책이 적용되는 방법을 연구한다.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
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• pp.962-964
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• 2003

In 2002 Thailand was faced with severe flooding in the North, Northeast and Central parts of the country caused by heavy rainfall of the monsoonal depression which brought about significant damages. According to the report by the Ministry of Interior and the Ministry of Agricultural and Co-operatives, the total damages were estimated to be about 6 billion bath. More than 850,000 farmers and 10 million livestock were effected. An area of 1,450,000 ha of farmland in 59 Provinces were put under water for a prolonged period. Satellite imageries were employed for mapping and monitoring the flood-inundated areas, flood damage assessment, flood hazard zoning and post-flood survey of river configuration and protection works. By integrating satellite data with other updated spatial and non-spatial data, likely flood zones can be predicted beforehand. Some examples of satellite data application to flood dis aster mitigation in Thailand during 2002 using mostly Radarsat-1 data and Landsat-7 data were illustrated and discussed in the paper. The results showed that satellite data can clearly identify and give information on the status, flooding period, boundary and damage of flooding. For comprehensive flood mitigation planning, other geo-informatic data, such as the elevation of topography, hydrological data need to be integrated. Ground truth data of the watershed area, including the water level, velocity, drainage pattern and direction were also useful for flood forecasting in the future.

• 한국전자통신학회논문지
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• 제13권6호
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• pp.1417-1424
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• 2018

구제역과 AI로 인한 피해는 해마다 반복되고 있으며 그 피해는 농가에서 그대로 떠안고 있는 실정이다. 현재 국내 축산농가의 외국인 노동력 수요가 급증하고 있으나 외국인 관리체계는 매우 미흡하며 자율적인 소독 의식이 부족하고, 계절적으로는 동절기 소독 문제가 심각한 상황이다. 오염물질로 피해를 보고 있는 중소 규모 농가와 경제적인 여건상 가격이 비싼 소독기를 설치할 여력이 없는 소규모 농가에서도 구매 설치 할 수 있는 출입관리 시스템이 필요하다. 본 논문에서는 저온 플라즈마 램프 원리를 이용하여 살균시스템을 도입하고 통합제어가 가능한 편리성 및 안전성 확보를 할 수 있는 ICT 융복합형 플라즈마 출입관리 시스템을 제안한다.

• 적정기술학회지
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• 제6권1호
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• pp.21-27
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• 2020

아프리카에서 시작돼 전 세계를 위협하는 ASF는 2018년 중국지역에서 발생해 아시아에 상륙하였고 2019년 5월 북한이 OIE에 ASF를 정식신고 함으로써 한반도 축산안보에 엄중한 위협으로 되었다. 1921년 영국의 수의병리학자 몽고메리는 동아프리카에서 풍토병으로 존재하던 치사율이 높은 바이러스에 의한 열성 돼지열병에 '아프리카돼지열병 African swine fever(ASF),'이라는 이름을 달아 세상에 알렸다. 아프리카지역에서 풍토병으로 유행하던 ASF는 1957년경 포르트갈에 상륙하여 포르투갈 리스본의 농장을 휩쓸었다. ASF는 계속하여 스페인과 포르투갈에 정착하여 1990년대 말까지 40년 동안 피해를 주었으며, 1978년에 이탈리아의 사르디니아섬에 상륙하여 현재진행형이다. 2007년에 흑해 연안의 죠지아 공화국의 포티항구에 상륙한 바이러스는 러시아까지 퍼졌고, 2018년에 중국에 침입하여 막대한 피해를 주면서 베트남, 라오스, 미얀마 등지로 급속히 퍼지다가 2019년 5월 북한에서 발병하여 전국에 확산되어 양돈업계에 막대한 피해를 주었으며 현재 진행형이다. 북한에서의 발병사실 확인후 불과 3개월 만에 2019년 9월 16일 파주와 연천의 농가에서 발병이 확인되면서 한국도 ASF발병국의 오명을 가지게 되었으며 ASF의 전국확산은 막았지만 야생 멧돼지에서 현재 진행형이다. 남북한에서 현재 진행중인 ASF가 토착화된다면 양돈산업은 물론 한반도 경제에 막대한 피해를 주는 역대 급 재앙으로 될 것이다. 현재의 상황상 ASF 발병으로 인한 위험요인을 배제하고, 남한지역에 국한된 수의방역에만 치중할 수 없다. 현재 북한의 열악한 수의방역현실로는 ASF발생으로 인한 양돈업계의 피해를 방지하기 어렵고, 지리적으로 각종 유행성 전염병의 발병위험이 높은 중국과 인접되어 있어 국경지역에 대한 검역과 방역을 남북한이 공동으로 진행하여야 할 필요성이 제기된다. 우선 한반도공동방역체계를 구축하기전 과제로 ASF 및 기타 질병에 대한 신속한 정보교류와 교육이 필요하다고 생각한다. 북한의 ASF발생지역 및 중국, 러시아 접경지역에 대한 철저한 방역과 소독을 실시하고 유통되는 가축 및 축산물에 대한 철저한 검역과 통제 등을 공동으로 진행하는 것이 중요하다. 모든 수단과 방법을 동원하여 한반도에서 ASF의 장기화를 막아 한반도 축산안보를 보장하기 위한 남북한 수의방역업계의 협력이 필수적이다.

• 한국동물위생학회지
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• 제28권2호
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• pp.121-146
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• 2005

Newcastle disease (ND), infectious bronchitis (IB), low pathogenic avian Influenza (LPAI) and fowl typhoid (FT) have been known as egg drop laying diseases because of the serious layer damage from mass zone layer. In this study, such egg drop laying diseases were investigated. To access this study, we peformed to evaluate antibody titers in serum and isolated bacteria and virus from organs and feces on May, July and September in 2003. The distribution of ND from January to May, IB and LPAI from October to February of the next year, and FT from March to September were inspected by the question survey in 21 farms. ND revealed to be positive rates of 490 to 474 $(96.7\%)$ in May, 510 to 506 $(99.2\%)$ in July and 510 to 510 $(100\%)$ in September with hemagglutination inhibition (HI) test. The mean antibody titers were 10.2, 9.9 and 10.2, respectively. With regard to IB, 484 out of 490 samples $(98.7\%)$ in May, 508 of 510 $(99.6\%)$ in July and 509 of 510 $(99.8\%)$ in September showed positive results and the mean antibody titers were gradually increased with 8.2, 9.0 and 9.4, respectively. According to HI test of LPAI, the positive results were shown in 442 of 480 $(92.1\%)$, 394 of 494 $(79.8\%)$ and 402 of 483 $(83.2\%)$ in May, July and September, respectively The mean antibody titers were decreased with 4.6, 4.3 and 4.0. The distribution of LPAI also elicited the positive rates of 480 to 475 $(99.0\%)$ in May, 494 to 485$(98.2\%)$ in July, 483 to 472 $(97.7\%)$ in September as determined by ELISA and the mean S/P ratio were 2.319, 2.557 and 2.380, respectively. Compared ELISA results with HI test of LPAI the positive results were 480 to 422 $(92.1\%),\;475(99.0\%),\;494\;to\;394 (79.8\%),\;485 (98.2\%)\;and\;483\;to\;402(83.2\%),\;472(97.7\%)$. Therefore, the positive rate determined by ELISA was higher than that of HI test with 6.9, 18.4 and $14.5\%$, respectively. When performed RT-PCR for ND using organ and feces samples, the pathotypes were detected $5(15.6\%)\;in\;May,\; 2(5.3\%) in\;July,\;2(7.1\%)$ in September but there is no samples showing positive band for LPAI. In attempt to isolate Salmonella gallinarum, bacteria were obtained from 4 cases (12.5%) in May, 9 (23.6%) in July, 5 (17.8%) in September. Thus the highest rate for isolation revealed to be shown in July When evaluated the antimicrobial susceptibility to 18 isolated strains of 5. gallinarum, bacteria were sensitive to trimethoprim/sulfamethox$(61.1\%),\;kanamycin\;(55.5\%),\;ampicillin\;(55.5\%)$ and amoxacillin/clavulanic acid $(55.5\%)$, cephalothin $(50.0\%)$, but resistant to penicillin $(88.9\%)$, streptomycin $(88.9\%)$, erythromycin $(83_4\%)$ and tetracycline $(61.1\%)$. According to HI test of ND and LPAI using captured 164 wild Korean tree sparrows (Passer nontanus), the positive rates were $47.6\%\;and\;57.3\%$, and the mean HI titers were 5.32 and 4.02, respectively. 71 $(43.2\%)\;and\;58(35.3\%)$ in captured sparrows also showed more than 4 titers for HI test to ND and LPAI, respectively However, the attempt for isolation of viruses failed in all samples.

• 한국초지조사료학회지
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• 제6권2호
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• pp.71-77
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• 1986

본(本) 시험(試驗)은 ladino clover(Trifolium repens L.)가 우점(優占)된 혼파초지(混播草地)에 제초제(除草劑) 사용(使用)이 식생구성(植生構成) 및 초지생산성(草地生産性)에 미치는 영향(影響)을 구명(究明)코자 축산시험장(畜産試驗場) 초지(草地) 시험포(試驗圃)에서 난괴법으로 4반복(反復)으로 $1983{\sim}'85$년간(年間) 실시(實施)되었다. 시험(試驗)에 사용(使用)된 공시초지(供試草地)는 클로버가 $60{\sim}65%$ 우점(優占)된 혼파초지(混播草地)로 제초제(除草劑)는 Banvel(100, 200cc), Hedonal(150, 300cc), U-46(150, 300cc) 및 Sinazin(100, 200g/10a)을 사용(使用)하였다. $1983{\sim}'85$년간(年間) 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. 1. Ladino clover가 우점(優占)된 혼파초지(混播草地)에 Banvel, U-46 및 Hedonal 약제(藥劑)를 처리(處理)함으로써 클로버 식생비율(植生比率)은 각각(各各) $1{\sim}2%$, $11{\sim}18%$ 및 $22{\sim}31%$로 감소(減少)되었다. 그러나 U-46 및 Hedonal 약제(藥劑)는 클로버에 대(對)한 살초력(殺草力)이 약(弱)하여 처리후(處理後) 2년차(年次)에는 클로버에 의(依)한 재우점현상(再優占現象)이 나타난다. Simazin은 기존(旣存) 화본과목초(禾本科牧草)에 대(對)한 약해(藥害)가 있어 사용(使用)이 어렵다. 2. 제초제(除草劑) 처리시기(處理時期)는 목초보파(牧草補播) $20{\sim}25$ 일전(日前)이 적합(適合)하다. 봄 또는 이른 여름에 약제처리시(藥劑處理時)에는 Digitaria sanguinalis, Echinochloa crus-galli 등(等)의 잡초발생(雜草發生)이 심(甚)하여 부실초지(不實草地)의 원인(原因)이 된다. 3. Banvel 약제(藥劑)의 토양잔류기간(土壤殘留期間)은 $7{\sim}10$일(日)로 이 기간내(期間內)의 목초파종(牧草播種)은 종자발아(種子發芽) 및 초기생육(初期生育)에 장해(障害)가 뒤따른다. 4. Ladino clover가 우점(優占)된 초지(草地)에 Banvel 약제(藥劑) 처리후(處理後) 화본과초지(禾本科草地)를 보파(補播)함으로서 년간(年間) 건물수량(乾物收量) 1023kg/10a이 생산(生産)되어 무처리구(無處理區)에 비(比)해 44%의 증수효과(增收效果)가 있었다. Hedonal 및 U-46 처리(處理)에서도 건물수량(乾物收量)은 각각(各各) 811kg 및 842kg/10a으로 현저(顯著)한 증가(增加)를 보였다. 5. 약제처리(藥劑處理)에 의(依)해 화본과위생형(禾本科爲主型) 초지(草地)로 개량(改良)함으로서 식생체내(植生體內)의 단백질함량(蛋白質含量) 및 net energy lactation(NEL)은 감소(減少)되었다. 그러나 총(總) NEL 수량(收量)은 각각(各各) 5401 MJ(Banvel), 4556 MJ(U-46) 및 4323 MJ (Hedonal)이 생산(生産)되어 clover 우점초지(優占草地) 3889 MJ/10a DM에 비(比)해 현저(顯著)한 증수효과(增收效果)가 있었다.

• Journal of Microbiology and Biotechnology
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• 제32권10호
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• pp.1253-1261
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• 2022

Staphylococcus aureus (S. aureus) infection causes dramatic harm to human health as well as to livestock development. As an important virulence factor, alpha-hemolysin (hla) is critical in the process of S. aureus infection. In this report, we found that bavachin, a natural flavonoid, not only efficiently inhibited the hemolytic activity of hla, but was also capable of inhibiting it on transcriptional and translational levels. Moreover, further data revealed that bavachin had no neutralizing activity on hla, which did not affect the formation of hla heptamers and exhibited no effects on the hla thermal stability. In vitro assays showed that bavachin was able to reduce the S. aureus-induced damage of A549 cells. Thus, bavachin repressed the lethality of pneumonia infection, lung bacterial load and lung tissue inflammation in mice, providing potent protection to mice models in vivo. Our results indicated that bavachin has the potential for development as a candidate hla inhibitor against S. aureus.

• Animal Bioscience
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• 제35권4호
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• pp.631-637
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• 2022

Objective: Surface disinfection is important in the proper running of livestock farms. However, disinfection of farm equipment and facilities is difficult because they are made of different materials, besides having large surface areas and complex structures. 3-(trimethoxysilyl)-propyldimethyloctadecyl ammonium chloride (Si-QAC) is a quaternary ammonium salt-based disinfectant that attaches to various surfaces by forming covalent bonds and maintains its disinfecting capacity for a considerable time. Our aim was to evaluate the potential use of Si-QAC for disinfection of farm equipment and facilities. Methods: The short- and long-term antimicrobial and antiviral effects of Si-QAC were evaluated in both laboratory and farm settings using modified quantitative assessment method based on the standard operating procedures of the United States Environmental Protection Agency. Results: Si-QAC was highly effective in controlling the growth of the Newcastle disease virus and avian pathogenic Escherichia coli. Electron microscopy revealed that the mechanism underlying the disinfection activity of Si-QAC was associated with its ability to damage the outer membrane of the pathogen cells. In the field test, Si-QAC effectively reduced viral contamination of surfaces of equipment and space. Conclusion: Our results suggest that Si-QAC has great potential as an effective chemical for disinfecting farm equipment and facilities. This disinfectant could retain its disinfection ability longer than other commercial disinfectants and contribute to better farm biosecurity.