• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.3
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• pp.211-216
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• 2016

Micronucleus test is genotoxicity assay for detection of micronuclei in the cytoplasm of interphase cells. The reduction and replacement of in vivo toxicity testing on animals require the development of in vitro models to predict the genotoxicity or other tests for cosmetic products. In this study, we evaluated a genotoxicity assay for topically applied chemicals using a three-dimensional human reconstructed skin model, KeraSkin$^{TM}$. Two genotoxins, mitomycin C (MMC) and methyl methanesulfonate (MMS), induced significant dose-related increases in cytotoxicity and micronuclei induction in the skin model. In contrast, two non-genotoxins, 4-nitrophenol (4-NP) and trichloroethylene (TCE), induced cytotoxicity but not micronucleus formation. In conclusion, micronucleus test using human skin model may be useful for predicting in vitro genotoxic potentials of cosmetic products.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.39 no.6
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• pp.507-513
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• 2021

GPR (Ground Penetrating RADAR) is a sensor that inspects the pavement state of roads, sinkholes, and underground pipes. It is widely used in road management. MMS (Mobile Mapping System) creates a detailed and accurate road map of the road surface and its surroundings. If both types of data are built in the same area, it is efficient to construct both ground and underground spatial information at the same time. In addition, since it is possible to grasp the road and important facilities around the road, the location of underground pipelines, etc. without special technology, an intuitive understanding of the site is also possible, which is a useful tool in managing the road or facilities. However, overseas equipment to which this latest technology is applied is expensive and does not fit the domestic situation. LiDAR (Light Detection And Raging) and GNSS/INS (Global Navigation Satellite System / Inertial Navigation System) were synchronized in order to replace overseas developed equipment and to secure original technology to develop domestic equipment in the future, and GPR data was also synchronized to the same GNSS/INS. We developed software that performs georeferencing using the location and attitude information from GNSS/INS at the time of acquiring synchronized GPR data. The experiments were conducted on the road site by dividing the open sky and the non-open sky. The road and surrounding facilities on the ground could be easily checked through the 3D point cloud data acquired through LiDAR. Georeferenced GPR data could also be viewed with a 3D viewer along with point cloud data, and the location of underground facilities could be easily and quickly confirmed through GPR data.

• Environmental Mutagens and Carcinogens
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• v.21 no.2
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• pp.89-94
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• 2001

Recently, single cell gel electrophoresis, also known as comet assay, is widely used for the detection and measurement of DNA strand breaks in vitro and in vivo in many toxicological fields such as radiation exposure, human monitoring and toxicity evaluation. As well defined, comet assay is a sensitive, rapid and visual method for the detection of DNA strand breaks in individual cells. Briefly, a small number of damaged cells suspended in a thin agarose gel on a microscope slide were lysed, unwinded, electrophoresed, and stained with a fluorescent DNA binding dye. The electric current pulled the charged DNA from the nucleus such that relaxed and broken DNA fragments migrated further. The resulting images which were subsequently named for their appearance as comets, were measured to determine the extent of DNA damages. However, some variations could be occurred in procedures, laboratories's conditions and kind of cells used. Hence, to overcome and to harmonize these matters in comet assay, International Workshop on Genotoxicity Test Procedure (IWGTP) was held with several topics including comet assay at Washington D.C. on March, 1999. In spite of some consensus in procedures and conditions in IWGTP, there are some problems still remained to be solved. In this respect, we attempted to set the practical optimal conditions in the experimental procedures such as lysis, unwinding, electrophoresis and neutralization conditions and so on. First of all, we determined optimal lysis and unwinding time by using 150 $\mu$M methyl methanesulfonate (MMS) which is usually used concentration. And then, we determined optimal positive control concentrations of benzo(a)pyrene (BaP) and MMS in the presence and absence of S9 metabolic activation system, respectively.

• Korean Journal of Microbiology
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• v.38 no.2
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• pp.96-102
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• 2002

The S-phase checkpoint mechanisms response to DNA damage or inhibition of DNA replication for maintenance of genetic stability in eukaryotic cells. These roles include cell cycle control arrest at S-phase and Iranscriptional induction of repair genes. To characterize the defects of dpbll mutant for both these responses, we examined the over-expression effect of DPBll gene, the sensitivity to HU, MMS, and the transcriptional pattern by DNA damage agent for RNRS mRNA. RNRS transcript is induced in response to a wide variety of agents that either damage D7A directly through chemical modification or induce stress by blocking DNA synthesis. As results, dpbll-1 cells are sensitive to DNA damage agents and the level of RNR3 mRNA is reduced approximately 40% than wild type cells. Moreover, we found the same results in dpb2-1 cells. Therefore, we propose that DPB2 and DPBll act as a sensor of replication that coordinates the transcriptional and cell cycle responses to replication blocks.

• Proceedings of the KOSOMBE Conference
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• v.1998 no.11
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• pp.100-101
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• 1998

In this paper, we've studied on the Breakdownpatterns which hove an effect on Down-Time of Medical Equipment. This study is based on the statistics of MMS(Maintenance Management System) accumulated since the opening of Samsung Medical Center. We hope that this paper, which is acquired through the analysis about the seasonal feature, the work pattern feature and the period used, is useful to reduce a term of Down-Time and to retrench hospital management cost.

• Journal of Magnetics
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• v.10 no.3
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• pp.113-117
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• 2005

Some polycrystalline ferromagnetic mms are composed of continuously connected nanometer scale islands with random crystallite orientations. The nanometer perturbations of the mm introduce a large number of nearly degenerate local field configurations that are indistiguishable on a macroscopic scale. As a first step, this situation is modelled as a thin ferromagnetic disc coupled by exchange and dipole interactions to a homogeneous ferromagnetic plane, where the disc and plane have different easy axes. The model is solved to find the partial $N\acute{e}el$ domain walls that minimize the magnetic energy. The two solutions give a bistable configuration that, for appropriate geometries, provides an important microsopic ferromagnetic degree of freedom for the mm. These results are used to interpret recent measurements of exchange biased bilayer films.

• The Transactions of the Korea Information Processing Society
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• v.5 no.9
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• pp.2233-2247
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• 1998

With the growth of World Wide Web, multimedia electronic documents have been frequently used in a lot of applications. Especially, digital libraries tliat deal with huge and various multimedia electronic documents need to support efficient searcli techniques and systematical management techniques. In this paper, we suggest an extended Dublin Core metadata to store and search the multimedia electronic documents managed at digital libraries. We also design a document database schema based on the extended Dublin Core metadata and implement a svstem, called xD-MMS which manages the multimedia electronic documents.

• Journal of Broadcast Engineering
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• v.10 no.4 s.29
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• pp.528-539
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• 2005

The H.264 has been adopted as the video codec for various multimedia services such as DMB and next-generation DVD because of its superior coding performance. However, the reference codec of the standard, the joint model (JM) contains quite a few algorithms which are too complex to be used for the resource-constraint embedded environment. This paper introduces very low-complexity H.264 encoding algorithm which is applicable for the embedded environment. The proposed algorithm was realized by restricting some coding tools on the basis that it should not cause too severe degradation of RD-performance and adding a few early termination and bypass conditions during the motion estimation and mode decision process. In case of encoding of 7.5fps QCIF sequence with 64kbpswith the proposed algorithm, the encoder yields worse PSNRs by 0.4 dB than the standard JM, but requires only $15\%$ of computational complexity and lowers the required memory and power consumption drastically. By porting the proposed H.264 codec into the PDA with Intel PXA255 Processor, we verified the feasibility of the H.264 based MMS(Multimedia Messaging Service) on PDA.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.36 no.6
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• pp.535-544
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• 2018

Autonomous driving can be limited by only using sensors if the sensor is blocked by sudden changes in surrounding environments or large features such as heavy vehicles. In order to overcome the limitations, the precise road-map has been used additionally. This study was conducted to segment and classify road objects using 3D point cloud data acquired by terrestrial mobile mapping system provided by National Geographic Information Institute. For this study, the original 3D point cloud data were pre-processed and a filtering technique was selected to separate the ground and non-ground points. In addition, the road objects corresponding to the lanes, the street lights, the safety fences were initially segmented, and then the objects were classified using the support vector machine which is a kind of machine learning. For the training data for supervised classification, only the geometric elements and the height information using the eigenvalues extracted from the road objects were used. The overall accuracy of the classification results was 87% and the kappa coefficient was 0.795. It is expected that classification accuracy will be increased if various classification items are added not only geometric elements for classifying road objects in the future.

• Journal of KIBIM
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• v.10 no.4
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• pp.32-39
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• 2020

In recent years, the construction industry is getting bigger and more complex, so it is becoming difficult to acquire point cloud data for construction equipments and workers. Point cloud data is measured using a drone and MMS(Mobile Mapping System), and the collected point cloud data is used to create a 3D digital map. In particular, the construction site is located at outdoors and there are many irregular terrains, making it difficult to collect point cloud data. For these reasons, adopting a noise reduction algorithm suitable for the characteristics of the construction industry can affect the improvement of the analysis accuracy of digital maps. This is related to various environments and variables of the construction site. Therefore, this study reviewed and analyzed the existing research and techniques on the noise reduction algorithm. And based on the results of literature review, performance evaluation of major noise reduction algorithms was conducted for digital maps of construction sites. As a result of the performance evaluation in this study, the voxel grid algorithm showed relatively less execution time than the statistical outlier removal algorithm. In addition, analysis results in slope, space, and earth walls of the construction site digital map showed that the voxel grid algorithm was relatively superior to the statistical outlier removal algorithm and that the noise removal performance of voxel grid algorithm was superior and the object preservation ability was also superior. In the future, based on the results reviewed through the performance evaluation of the noise reduction algorithm of this study, we will develop a noise reduction algorithm for 3D point cloud data that reflects the characteristics of the construction site.