• Journal of Food Hygiene and Safety
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• v.35 no.4
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• pp.319-325
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• 2020

Widespread consumption of fresh-cut vegetables without cooking results in ingestion of major foodborne pathogens including Bacillus cereus. In this study, we aimed to develop a method to rapidly detect B. cereus in fresh-cut vegetables by combining commercial PCR analysis with enrichment of the pathogenic levels. A mixture of B. cereus strains (KCTC1013, KCTC1014, KCTC1092, KCTC1094, and KCTC3624) was inoculated on the surface of fresh-cut cabbage lettuce (20 g) and baby leafy vegetables (10 g) to concentration 1, 2, 3, 4, and 5 log CFU/g. Eighty milliliters of TSB with 0.15% polymyxin B was used for cabbage lettuce, and 90 mL of medium was used for baby leafy vegetables and incubated at 42℃ for 0, 2, 3, 4, 5, 6, and 7 h. One milliliter of the enriched media was plated on mannitol-egg yolk-polymyxin agar for quantification, and another 1 mL was used for DNA extraction for PCR analysis. Additionally, the minimum number of sub-samples to be tested from a pack of fresh-cut vegetable samples was determined using 5 sub-samples. The results from this study showed that for detecting B. cereus in fresh-cut cabbage lettuce, 3, 4, 5, 6, and 7 h enrichment were required to at least detect 5, 4, 3, 2, and 1 log CFU/g of B. cereus, respectively. B. cereus in fresh-cut baby leafy vegetables could be detected after 2, 3, 4, 5, and 6 h of enrichment at 5, 4, 3, 2, and 1 log CFU/g, respectively, using a combination of enrichment and PCR analysis. To determine if a pack of fresh-cut vegetable is positive, the minimum number of sub-samples should be 3. These results can be used to develop a rapid detection method to semi-quantify B. cereus in fresh-cut vegetable samples combining enrichment and PCR.

• Journal of the Korean institute of surface engineering
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• v.54 no.5
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• pp.278-284
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• 2021

Corrosion of steel plate is common phenomenon which results in the gradual destruction caused by a wide variety of environments. Corrosion monitoring is the tracking of the degradation progress for a long period of time. Corrosion on steel plate appears as a discoloration and any irregularities on the surface. In this study, we developed a quantitative evaluation method of the rust formed on steel plate by using k-means clustering from the corroded area in a given image. The k-means clustering for automated corrosion detection was based on the GrabCut segmentation and Gaussian mixture model(GMM). Image color of the corroded surface at cut-edge area was analyzed quantitatively based on HSV(Hue, Saturation, Value) color space.

• Food Science of Animal Resources
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• v.34 no.5
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• pp.665-673
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• 2014

We compared standard culture methods as well as conventional PCR and real-time PCR for the detection of Listeria monocytogenes (L. monocytogenes) in milk, cheese, fresh-cut vegetables, and raw beef that have different levels of background microflora. No statistical differences were observed in sensitivity between the two selective media in all foods. In total, real-time PCR assay exhibited statistically excellent detection sensitivity (p<0.05) and was less time consuming and laborious as compared with standard culture methods. Conventional culture methods showed poor performance in detecting L. monocytogenes in food with high levels of background microflora, generating numerous false negative results. While the detection of L. monocytogenes in fresh cut vegetable by culture methods was hindered only by L. innocua, various background microflora, such as L. innocua, L. welshimeri, L. grayi, and Enterococcus faecalis appeared on the two selective media as presumptive positive colonies in raw beef indicating the necessity of improvement of current selective media. It appears that real-time PCR is an effective and sensitive presumptive screening tool for L. monocytogenes in various types of foods, especially foods samples with high levels of background microflora, thus complementing standard culture methodologies.

• Journal of IKEEE
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• v.15 no.1
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• pp.57-63
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• 2011

This paper presents a tunable band pass filter (BPF) for multi bio-signal detection. The bandwidth can be controlled by the bias current of transconductance (gm), while conventional BPF exploited switchable capacitor array for band selection. With this design technique, the die area of proposed BPF reduced to at least one tenth the area of conventional design. The simulation results show the high cut-off frequency tuning range of from 100Hz to 1Khz. The circuit was implemented with a 0.18um CMOS standard technology. Total current consumption is 1uA at the supply voltage of 1V with sub-threshold design technique.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.16
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• pp.7381-7384
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• 2015

This study was conducted to 1206 women who had cervical cancer screening at Chonburi Cancer Hospital. The spilt-sample study aimed to compare the efficacy of abnormal cervical cells detection between liquid-based cytology (LBC) and conventional cytology (CC). The collection of cervical cells was performed by broom and directly smeared on a glass slide for CC then the rest of specimen was prepared for LBC. All slides were evaluated and classified by The Bethesda System. The results of the two cytological tests were compared to the gold standard. The LBC smear significantly decreased inflammatory cell and thick smear on slides. These two techniques were not difference in detection rate of abnormal cytology and had high cytological diagnostic agreement of 95.7%. The histologic diagnosis of cervical tissue was used as the gold standard in 103 cases. Sensitivity, specificity, positive predictive value, negative predictive value, false positive, false negative and accuracy of LBC at ASC-US cut off were 81.4, 75.0, 70.0, 84.9, 25.0, 18.6 and 77.7%, respectively. CC had higher false positive and false negative than LBC. LBC had shown higher sensitivity, specificity, PPV, NPV and accuracy than CC but no statistical significance. In conclusion, LBC method can improve specimen quality, more sensitive, specific and accurate at ASC-US cut off and as effective as CC in detecting cervical epithelial cell abnormalities.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.11
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• pp.196-201
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• 2016

Digital watermarking is a technique used to hide information within digital media. Digital watermarking techniques can be classified as either robust watermarking or fragile watermarking. Robust watermarking techniques are generally used for the purpose of copyright protection. In addition, fragile watermarking techniques are used for the authentication and integrity verification of a digital image. Therefore, fragile watermarks should be easily breakable for trivial tampering of a watermarked image. This paper proposes an efficient fragile watermarking method for image tamper detection in the spatial domain. In the proposed method, a hash code and symmetric key encryption algorithm are used. The proposed method of inserting a watermark by dividing the original image into many blocks of small sizes is not weak against attacks, such as cut and paste. The proposed method can detect the manipulated parts of a watermarked image without testing the entire block of the image.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.9C
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• pp.1269-1278
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• 2004

While the management of digital contents is getting more and more important, many researchers have studied about scene change detection algorithms to reduce similar scenes in the video contents and to efficiently summarize video data. The algorithms using histogram and pixel information are found out as being sensitive to light changes and motion. Therefore, visual rhythm gets used in recent work to solve this problem, which shows some characteristics of scenes and requires even less computational power. In this paper, a new scene detection algorithm using visual rhythm by direction is proposed. The proposed algorithm needs less computational power and is able to keep good performance even in the scenes with motion. Experimental results show the performance improvement of about 30% comparing with conventional methods with histogram. They also show that the proposed algorithm is able to keep the same performance even to music video contents with lots of motion.

• Journal of the Korean Data and Information Science Society
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• v.27 no.1
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• pp.131-142
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• 2016

Outliers in surveys can have a large effect on estimates of totals. This is especially true in business surveys where the populations are drawn are typically skewed. In this paper, we discussed the practical development and implementation of methods to identify and deal with outliers. A detection method is based on quartile method and detected outlier is processed in various ways. The study examines two versions of winsorised estimators with three different cut-off thresholds for each one. For the simulation study, four types of weight transformation function have been considered.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.9
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• pp.2223-2228
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• 2014

Available shafts with gears at each end of the cylinder-shaped steel are widely used as power train components for automobiles. In order to automate the production of a geared shaft, there are problems to be solved. After one side of the cylinder is cut in gears, one of the problems is to determine the position of the cylinder which has been already cut. The shaft is then to be fed ahead with geared position to the chuck with jaws in geared shape. The other problem is to acquire information on an angular position of the shaft to fit to the jaws of the chuck. This paper deals with the magnetic detection sensor of gear position. Coils are installed in two places. Self-inductances of coils are detected by the changes of reluctance and are then compared. The magnetic analysis also has been carried out by the finite element analysis (FEM).

• Applied Biological Chemistry
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• v.49 no.3
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• pp.254-258
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• 2006

Vitellogenin has been known as a potent biomarker protein for the estrogenic activity in fish exposed to endocrine disruptors. In this study, a piezoelectric immunosensor making use of an anticarp vitellogenin antibody and an AT-cut quartz crystal microbalance as the biological component and transducer was prepared, followed by its application to the analysis of carp vitellogenin as follows. Antibody immobilization was conducted by chemisorption of a thiolated antibody with a heterobifunctional thiolation cross-linker, sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate. The reaction buffer for the immunosensor system was optimized as 0.1 M sodium phosphate (pH 7.4). Concentration-dependent sensor responses were obtained in the vitellogenin concentrations ranging from 0.4864 to 486.4000 nM, with a linear correlation between vitellogenin concentration and frequency shift in double-logarithmic scale. The limit of detection of the immunosensor for carp vitellogenin was presumed as 0.4864 nM.