• 제목/요약/키워드: curvilinear

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Motional kinematics of Frozen-thawed Korean native cattle semen use of computer aided semen analysis(CASA) system (컴퓨터 정액자동분석에 의한 동결융해 한우 정액의 운동특성 연구)

  • Lee, Kang-nam;Lee, Byeong-chun;Kim, Jung-tae;Park, Jong-im;Shin, Tae-young;Hwang, Woo-suk
    • Korean Journal of Veterinary Research
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    • v.38 no.4
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    • pp.898-908
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    • 1998
  • The aim of this experiments were to assess the time-interval change of motional characteristics in frozen-thawed semen of Korean native cattle (KNC) by using computer aided semen analysis (CASA) technology. Twenty-six KNC frozen semen straws were obtained from Korean KNC improvement department, livestock improvement main division, national livestock cooperatives federation in Korea. Specimens were allowed to thaw at $37^{\circ}C$ for 30 sec in water bath. Semen analysis was performed on semen image analysis system (SIAS, Medical supply, Korea) adjusted to the gate settings and used the semen droplet ($5{\mu}l$) placed on Makler counting chamber (Sefi medical instrument, Israel) prewarmed at $37^{\circ}C$. The same person used the same micropipette to fill the Makler counting chamber. A total of 150 or more of sperms were analysed in each specimen by a single trained person by scanning at least 5 to 10 fields. The measurement parameters in SIAS were as follows ; frame rate = 30 frames per sec, image capture = 1 sec, minimum motile speed = $10{\mu}m/s$, maximum countable sperm number = 400. Statistical analysis was done by Student t-test with use of the Sigma plot program on a IBM personal computer. The dancemean(DNM) and hyperactivated sperm(HYP) of frozen-thawed KNC semen kinematics were significantly decreased(p < 0.05) after 10 min of incubation at $37^{\circ}C$ water bath. But, wobble(WOB) of same sample semen was significantly increased(p < 0.05) after 10 min of incubation and significantly decrease(p < 0.05) after 60 min of same incubation. And, after 30 mim of incubation, significantly differences were found most of motion kinematics, motifity(MOT), curvilinear velocity(VCL), straight line velocity(VSL), average path velocity(VAP), amplitude of lateral head displacement(ALH), beat cross frequency(BCF), mean angular displacement(MAD), dance(DNC), on same sample semen. The DNM of KNC semen sample was variable kinematics after 30 min of incubation. Also, the linearity(LIN) and straightness(STR) was significantly decreased(p < 0.05) from 60 min of incubation. In conclusion, the AI within 30 min after thawing of frozen semen can be an effective method for obtaining high fertility rate in KNC reproductive program.

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Association study analysis of phospholipase C zeta gene polymorphism forsperm motility and kinematic characteristics in liquid semen of Boar (Phospholipase C zeta 유전자의 유전적다형성과 돼지 액상정액의 운동학적 특성과의 연관성 분석)

  • Jeong, Yong-dae;Jeong, Jin-Young;Sa, Soo-Jin;Kim, Ki-Hyun;Cho, Eun-Seok;Yu, Dong-Jo;Park, Sungk-won;Jang, Hyun-Jun;Woo, Jae-Seok;Choi, Jung-Woo
    • Journal of Embryo Transfer
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    • v.31 no.3
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    • pp.293-297
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    • 2016
  • For evaluating the boar semen quality, sperm motility is an important parameter because the movement of sperm indicates active metabolism, membrane integrity and fertilizing capacity. Phospholipase C zeta (PLCz) is important enzyme in spermatogenesis, but the effect has not been confirmed in pigs yet. Therefore, this study was aimed to analyze their association with sperm motility and kinematic characteristics. DNA samples from 124 Duroc pigs with records of sperm motility and kinematic characteristics [total motile spermatozoa (MOT), curvilinear velocity (VCL), straight-line velocity (VSL), the ratio between VSL and VCL (LIN), amplitude of lateral head displacement (ALH)] were subjected. A SNP in non-coding region of PLCz g.158 A > C was associated with MOT (p < 0.05), VCL (p < 0.01), LIN (p < 0.01) and ALH (p < 0.05) in Duroc population. Therefore, we suggest that the intron region of the porcine PLCz gene may be used as a molecular marker for Duroc boar semen quality, although its functional effect was not defined yet. Whether the association is due to the candidate gene or not require further verification. Thus, it will be of interest to continue association studies in the regions surrounding those genes.

Association study analysis of CD9 as candidate gene for Duroc pig sperm motility and kinematic characteristics (두록 정자 운동학적 특성과 후보유전자 CD9 유전자와의 연관성 분석)

  • Jeong, Yong-dae;Jeong, Jin-Young;Kim, Ki-Hyun;Cho, Eun-Seok;Yu, Dong-Jo;Choi, Jung-Woo;Jang, Hyun-Jun;Park, Sungk-won;Sa, Soo-Jin;Woo, Jae-Seok
    • Journal of Embryo Transfer
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    • v.31 no.3
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    • pp.281-285
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    • 2016
  • Cluster-of-differentiation antigen 9 (CD9) gene expressed in the male germ line stem cells is crucial for sperm-egg fusion, and was therefore selected as a candidate gene to investigate Duroc boar semen motility and kinematic characteristics. This study was performed to investigatetheir association with semen motility and kinematic characteristics. DNA samples from 96 Duroc pigs with records of sperm motility and kinematic characteristics [Total motile spermatozoa (MOT, $82.27{\pm}5.58$), Curvilinear velocity(VCL, $68.37{\pm}14.58$), Straight-line velocity(VSL, $29.06{\pm}6.58$), the ratio between VSL and VCL(LIN, $47.36{\pm}8.42$), Amplitude of Lateral Head displacement(ALH, $2.88{\pm}0.70$)] were used in present study. A single nucleotide polymorphism (g.358A>T) in intron 6 was associated with MOT, VCL, VAP and ALH in Duroc population (p<0.05). Therefore, we suggest that the porcine CD9 may be used as a molecular marker for Duroc boar semen quality, although its functional effect was not clear yet. These results will improve the understanding of the functions of the CD9 in spermatogenesis within the reproductive tracts, and will shed light on CD9 as a candidate gene in the selection of good sperm quality boars.

Ecology of Ginger Rhizome Rot Development Caused by Pythium myriotylum (Pythium myriotyrum에 의한 생강뿌리썩음병의 발생상태)

  • Kim, Choong-Hoe;Yang, Sung-Seok;Hahn, Ki-Don
    • Korean Journal Plant Pathology
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    • v.13 no.3
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    • pp.184-190
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    • 1997
  • Lesion enlargement of ginger rhizome rot was most rapid at 35~40 C, but delayed greatly as temperature decreased. Time needed for a killing a ginger plant, 22~25 cm long, was about 5 days at 35~40 C, but was 15 days at 15 C in a growth chamber test. Higher RH above 90%, higher soil moisture level above 80% of maximum soil moisture capacity, and deeper planting below 4cm enhanced the lesion development on ginger stems and rhizomes. Pythium myriotylum existed in field soil as forms of hyphal portion, hyphal swelling body, or oospore- or zoospore-like bodies, and served as the origin of its colonization. Inocula of P. myriotylum was randomly distributed in soil surface around ginger plants, but its density was decreased as increasing soil depth with the highest density at 0~10 cm soil depth. Population density of P. myriotylum did not vary significantly between the rhizoplane and the rhizosphere soil of a ginger plant, but differed greatly between the disessed and healthy plants with several to several hundreds times higher population in the diseased plants. A positive curvilinear relationship was found between P. myriotylum density and ginger rhizome rot severity.

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A Study on the Characteristics of Knitwear Fashion Design: With a focus on Missoni, Sonia Rykiel, Azzedine Alaia

  • Chun, Hei Jung;Park, Jae Min
    • International Journal of Costume and Fashion
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    • v.13 no.1
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    • pp.23-34
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    • 2013
  • The purpose of this study is to better understand the development and characteristics of knitwear fashion design by examining the transformation process of the modern knits. The subjects of the study are Missoni, Sonia Rykiel, and Azzedine Alaia, designers who are world-renowned knit designers, and the expressive techniques in their designs will be evaluated. The study also intends to analyze the aesthetic value of each designer's style through their product's silhouette, colors, and knitting techniques. On the basis of the analysis, we hope to research the factors in the designing process that will allow knits, which were made for practical purposes, to be valued as a luxury fashion item, and with the results, show the potential for knits in expanding its domain in fashion to become a more luxurious, creative fashion item. The characteristic comparison of the designers is as follows: First, in the case of colors, Missoni shows its distinct identity through a balance of splashy colors as well as nature-inspired color composition and balance. And, only with color use, is also able to express perspective, form composition, and rhythm. Sonia Rykiel designs are composed of black backgrounds with strong primary colors that are contrasted with one-point or stripes to express a light, urban image. Alaia emphasizes femininity by the use of black and white colors, which show modernity, in combination with neutral skin-toned colors, such as beige and gray. So, in other words, Missoni and Sonia Rykiel mixed colors for visual interconnectivity, while Alaia expressed femininity through the use of an achromatic color. Second, in the case of knitting techniques, Missoni uses the jacquard technique to make complex patterns that show balance of colors and patterns such as zigzag, stripe, geometries, and titan check, which are geometric, abstract, and symmetric. Sonia Rykiel who uses stripes as her trademark, most often utilizes the intarsia technique, which is expressed through one-point. Alaia combines diverse techniques, such as the Skashi weaving, by using computerized knitting. Third, as for silhouettes, Missoni eliminated exaggerated details in order to emphasize the flashy colors and delicate patterns and weavings of its designs, and this resulted in simplistic and relaxed silhouettes. Sonia Rykiel took advantage of the elasticity that the knit offers to get a tight silhouette, and in turn, emphasized the female sensuality. Alaia used curvilinear cuts that emphasized the womanly curves and gained an image considered soft and feminine.

Analysis of Short-Term and Long-Term Characteristics of GPS Satellite Clock Offsets (GPS 위성시계오차의 장단기 특성 분석)

  • Son, Eun-Seong;Park, Kwan-Dong;Kim, Kyeong-Hui
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.28 no.6
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    • pp.563-571
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    • 2010
  • The GPS satellite has three or four atomic clocks that consist of cesiums and rubidiums and the NANU messages can be used to identify the kind of the onboard atomic clock because they classify the clock type on a daily basis. In this study, for long-term analysis of the GPS satellite clock behavior, we extracted satellite clock errors for every PRN from years 2001 through 2009 using the SP3 files that are provided by the IGS. As a result, the cesium clock offsets usually have a linear trend of drifting. On the other hand, rubidium offsets show curvilinear variations in general, even though they cannot be represented as anyone specific polynomial function. For short-term analysis, we extracted satellite clock errors for each PRN for a week-long period using the CLK files that are also provided by the IGS and curve-fitted them with first-order and second-order polynomial functions. In cases of cesium clock errors, they were well-represented by first-order polynomial functions and rubidium clock errors were similar with second-order polynomials. However, some of rubidium clock errors could not be represented as any polynomial fitting function. To analyze the characteristic of GPS satellite by each block and atomic clock, we applied Modified Allan Deviation criterion to the dataset from years 2007 and 2010. We found that the Modified Allan Deviation characteristics changed significantly according the block and atomic clock type.

A Distcussion on the Size Deviation Test of Raw Silk (生絲纖度檢査規定에 關한 考察)

  • Choi, Jin-Sub
    • Journal of Sericultural and Entomological Science
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    • v.25 no.1
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    • pp.44-50
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    • 1983
  • The study was carried out to suggest on opinion of the standardization of size deviation in the existing raw silk testing method. 1. The present grade A of size deviation stipulates 4.61 to 5.80 of standard deviation for 50 to 69 denier of mean value, however, the 5.80 standard deviation with the mean values of 50 and 69 denier belong to different distribution. 2. It is reasonable that the variation coefficients of grade 2A should be lower than that of grade A. However, the present testing method shows larger variation in grade 2A than in grade A. This is illustrated 9.00 for 69 denier in grade 2A and 8.41 for 70 denier in grade A. 3. The size deviation value compares the quality of raw silk with different mean value. Therefore, the standard deviation is recommended to be replaced by the C.V. value in determining the grade of silk. 4. The C.V. have a tendency to increase with lower grades below 6A for the size deviation below 33 denier with some inconsistencies. The figures should be adjusted so that the C.V. inconsistencies size deviation below 33 denier will be corrected. 5. The standard deviation increases with size under the same grade for the size deviations above 33 denier, however, the C.V. does not vary greatly with size deviation. 6. To rectify the above-mentioned inconsistencies the C.V. conversion and curvilinear regresion correction is recommended to improve the present silk testing method 7. The table of size deviation standard are as follows: Suggested standard of size deviation, unit: C.V. Value

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Correlation of Motion Kinematics of Spermatozoa and Litter Size in Porcine (돼지 정자의 운동역학 변수와 산자수 간의 상관 관계)

  • Kwon, Woo-Sung;Ryu, Je-Sun;Park, Joo Wan;Kim, In Cheul;Kim, Jin;Rahman, Md. Saidur;Yoon, Sung-Jae;Park, Yoo-Jin;You, Young Ah;Pang, Myung-Geol
    • Reproductive and Developmental Biology
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    • v.36 no.3
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    • pp.189-192
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    • 2012
  • Male factor infertility or sub-fertility contributed half of all cases of infertility while the semen abnormality is the current topic of argument. Conventional analysis of semen showed poor correlation with fertility. Therefore, evaluation of current semen analysis method is necessary to improve standards of semen assessment. The goal of this study was to investigate that correlation between motion kinematic before and after capacitation and litter size in porcine. Sperm motility and kinematics were measure by computer-assisted sperm analysis (CASA). The motility of spermatozoa was positively correlated with curvilinear velocity (VCL), average path velocity (VAP), and mean amplitude of head lateral displacement (ALH) (p<0.05). Where as VCL positively correlated with VSL, VAP and ALH (p<0.01). Straight-line velocity (VSL) was positively correlated with VAP and ALH (p<0.01). VAP was significantly positively correlated with ALH (p<0.01). Also, we found significant positive correlation among variation of VSL, VAP and ALH (p<0.05). No motility and kinematic parameter are correlated with litter size. However, litter size was significantly correlated with breed (p<0.05). Our results suggested that analysis of sperm motility and kinematics using CASA is questionable for prediction of litter size. However, it has some practical importance to evaluate semen commercially.

Effect of Cysteine and Glutathione on Motility Index and Morphology in Human Spermatozoa (Cysteine 및 Glutathione이 사람정자의 운동성지수와 정자형태에 미치는 영향)

  • 윤정임;한만희;전은숙;허영문;이종인;이규승
    • Journal of Embryo Transfer
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    • v.15 no.3
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    • pp.211-218
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    • 2000
  • This experiment was conducted to investigate the effect of the cysteine and glutathione on the motility index and morphology of human spermatozoa at the sperm processing in vitro. After treating the sperm with medium containing cysteine and glutathione, we measured the motility index and morphology at 0.5 h and 24 h. 1. Following the sperm culture for 0.5 h after treating the sperm with the medium containing 0, 1, 5, 10 mM cysteine, curvilinear velocity (VCL) was significantly (p<0.05) higher in control than that in all treatments. And straight-line velocity (VSL) was high at 1 mM and average path velocity (VAP) was low at 5 mM and 10 mM. But the motility (MOT) and morphology (NOM) were not different between control and all treatments. Following the sperm culture for 24 h, the MOT was significantly high in treatment groups (58.9, 74.4 and 62.3%), compared with that in control(28.7%) and the VCL was also high in treatment groups (31.4, 37.9, and 34.0 ${\mu}{\textrm}{m}$/s), compared with that in control (21.3 ${\mu}{\textrm}{m}$/s). The VSL (18.4, 21.7, and 18.9 ${\mu}{\textrm}{m}$/s) was significantly higher than control (10.7 ${\mu}{\textrm}{m}$/s) and the VAP (20.3, 24.7, and 21.4 ${\mu}{\textrm}{m}$/s) in treatments was also compared with that in control (12.6 ${\mu}{\textrm}{m}$/s). The NOM was not difference between control and treatments. 2 Following the sperm culture for 0.5 after treating the sperm with the medium containing 0, 1, 5, 10 mM glutathione, the MOT, VCL, VSL, VAP, and NOM were not different between control and treatments. Following the sperm culture for 24 h, the MOT was higher in treatment groups (82.9, 83.6, 83.4%) than in control (51.1%) and the VCL was higher in treatment groups (50.9, 51.3, and 49.4 ${\mu}{\textrm}{m}$/s) than control (34.1 ${\mu}{\textrm}{m}$/s). The VSL was also higher in treatment (17.1 ${\mu}{\textrm}{m}$/s) and the VAP was also higher in treatment groups (30.1, 32.5, and 29.7 ${\mu}{\textrm}{m}$/s) than in control (19.8 ${\mu}{\textrm}{m}$/s). The NOM was not different between control and treatments.

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Objective Identification of Human Sperm Hyperactivation by Computerized Sperm Motion Analysis (Computerized Sperm Motility Analyzer를 이용한 Human Sperm의 Hyperactivated Motility의 객관적 관찰에 관한 연구)

  • Lee, Hee-Kyung;Lee, Chan;Kim, Hyun-Sook;Kim, Young-Tae;Kim, Sun-Haeng;Ku, Pyoung-Sahm
    • Clinical and Experimental Reproductive Medicine
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    • v.21 no.1
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    • pp.1-11
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    • 1994
  • The occurrence and time course of capacitation, acrosomal loss, and hyperactivated motility require quantitative definition in order to characterize fertile human sperm. Recently the method has been developed to estimate the quality of spermatozoa by using kinematic parameters such as curvilinear velocity(VCL), average path velocity(VAP), linearity(LIN), straightness(STR), amplitude of lateral head displacement(ALH), and beat cross frequence(BCF) from Computer Assisted Sperm Analysis (CASA). In this study, using the Hamilton Thorn motility analyzer HTM 2030(Hamilton Thorn Research, Beverly, MA), we attempted to identify the spermatozoa with hyperactivated motility (HA) objectively and to monitor hyperactivation of human spermatozoa during incubation in capacitating media and after treatment of calcium ionophore as compared with acrosome status. And we examined whether HA are related to the result of SPA. Semen samples obtained from 16 healthy men were prepared by swim up technique and preincubated in a capacitating media(modified BWW medium) for 5 hours and treated with calcium ionophore solution. The acrosome reaction was detected with PSA-FITC labelling of the acrosome and in vitro sperm ferilizing capacity was assessed by the zona free hamster ovum penetration assay (SPA). The incidence of hyperactivated sperm was 2.6% in fresh semen, 14.3% of the swim up population, 13.7% after 5h of incubation. Significant increase of percentage of hyperactivated sperm was observed after the incubation (p<0.05) but after treatment, no significant changes of percentage of hyperactivated sperm(l1.8%) in contrast to significant rise in the percentage of acrosome reacted cells. Correlation analysis failed to show any significant relationship between the percentage of sperm with HA and SPA score. In conclusion, although no direct correlations were found between the results of SPA and HA, hyperactivation of sperm is associated with capacitation and monitoring hyperactivated sperm will be expected as a method of evaluating the functional quality of sperm such as SPA.

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