• Title/Summary/Keyword: curcuma

Search Result 352, Processing Time 0.026 seconds

Curcumin Inhibits Phorbol Ester-induced Expression of Cyclooxygenase-2 in Mouse Skin through Suppression of Extracellular Signal-Regulated Protein Kinase Activity and NF-$\kappa$B Activation

  • Chun, Kyung-Soo;Surh, Young-Joon
    • Proceedings of the PSK Conference
    • /
    • 2003.10b
    • /
    • pp.147.3-148
    • /
    • 2003
  • Recently, there have been considerable efforts to search for naturally occurring substances for the intervention of carcinogenesis. Curcumin, a yellow coloring ingredient of turmeric (Curcuma longa L., Zingiberaceae), has been shown to inhibit experimental carcinogenesis and mutagenesis, but molecular mechanisms underlying its chemopreventive activities remain unclear. In the present work, we found that curcumin inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced expression of CPX-2 in female ICR mouse skin when applied topically 30 min prior to TPA as determined by both immunoblot and immunohistochemical analyses. (omitted)

  • PDF

A Study on the Stabitity and Dyeing Condition in the Curcuma Longa L. (울금의 색소 추출과 안정성에 관한 연구)

  • 소황옥
    • Journal of the Korean Society of Costume
    • /
    • v.39
    • /
    • pp.79-89
    • /
    • 1998
  • This study was carried out the effect of stability and color extract for it's condition in the curcuma L.. dyeing. The stability is to investigate the absorbance of the curcumin, one of the major yellow pigments and the stability regarding the effect of light, oxygen temperature and pH. The dyeing condition is compared the effect of mordanting condition and the best way to extract pigment and analysed through the color-fastness rating, color-difference value test. The main results obtained are summarized as follows ; 1.The best and proper solvent to extrect curcumin pigment was a ethanol and a distilled water. 2. The light effect indicated that the absorbances of solution in absence of ligh was more stable. 3. The oxygen(O2) effect to curcumin show-ed that the condition in the absence of O2 was more stable than that in presence of O2 4. The temperature showed that the absorbnace was best stable in4$^{\circ}C$ and less changed at $25^{\circ}C$ 5. The curcumin-etanol solution was stable in pH 2~4. 6. Generally color-fastness rating to silk, wool and cotton indicated that crocking C.F. and perspiration C.F. were more than 3rd grade and dry cleaning C.F. was more than 4th grade. But light color-fastness and washing color-fastness were very poor. 7. To make good color fastness, the mordan-ting treated group and the pre-mordant conditions were more effective than others 8. When compared with color-difference value test indicated that the silk was looks like more reddish and bluish color and than the wool and cotton were greenish and bluish. As a mordant, A(C2H4OH(COOH3) and D(K2Cr2O7)were more effective to make green-ish color in the silk and the reddish color was abtained by B(Al.K(SO4)2.12H2O) and C(FeSO4.7H2O).

  • PDF

Radical Scavenging and Anti-obesity Effects of Various Extracts from Turmeric (Curcuma longa L.) (추출용매에 따른 울금 추출물의 라디칼 소거능 및 항비만 효과)

  • Park, Jeongjin;Lee, Jeongmin;Jun, Woojin
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.42 no.12
    • /
    • pp.1908-1914
    • /
    • 2013
  • In the present study, the levels of phenolic compounds and flavonoids, as well as activities of radical scavenging (DPPH and ABTS radical scavenging activity) and anti-obesity were assessed with cold water (CLC), hot water (CLH), and methanolic (CLM) extracts of Curcuma longa L. (turmeric). Our results showed that the phenolic compounds of CLC, CLH and CLM were $3.68{\pm}0.80%$, $3.94{\pm}0.74%$ and $9.01{\pm}0.73%$, respectively. The DPPH and ABTS radical scavenging activities of the CLM were significantly higher than that of the water extracts (CLC and CLH). During the adipocyte differentiation, the treatment of CLM more significantly inhibited lipid accumulation in 3T3-L1 cells than that of the water extracts. These results indicate that the stimulation of radical scavenging potential and the inhibition of adipogenesis were brought on by the lipophilic compounds of turmeric.

Hepatoprotective Activities of Curcumin, Demethoxycurcumin and Bisdemethoxycurcumin (Curcumin, demethoxycurcumin 및 bisdemethoxycurcumin의 간보호 작용)

  • Cheon, Ho-Jun;Park, Jin-Goo;Kim, Yeong-Shik;Kang, Sam-Sik;Chi, Xing-Fu;Lee, Jung-Joon;Lee, Sun-Mee
    • Korean Journal of Pharmacognosy
    • /
    • v.38 no.2 s.149
    • /
    • pp.139-147
    • /
    • 2007
  • The aim of this study was to investigate the protective activity of curcumin, demethoxycurcumin and bisdemethoxycurcumin, isolated from Curcuma longa Linne, on hepatocyte injury induced by carbon tetrachloride (CCl$_4$,10 mM), t-butyl hydroperoxide (TBH, 0.5 mM) and D-galactosamine (GaIN,30 mM). Primary cultures of rat hepatocyte (18 h culture) were treated with CCl$_4$, TBH or GaIN and various concentrations (0.1, 1, 10 and 100 ${\mu}$M) of curcumin, demethoxycurcumin and bisdemethoxycurcumin CCl$_4$ significantly increased the levels of lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The increases in LDH, ALT and AST levels were inhibited by curcumin. Demethoxycurcumin and bisdemethoxycurcumin decreased the levels of AST. Curcumin inhibited the increases in ALT and AST levels induced by TBH. The increased levels of LDH, ALT and AST induced by TBH were inhibited by bisdemethoxycurcumin. GaIN markedly increased the levels of LDH, ALT and AST. These increases were significantly inhibited by bisdemethoxycurcumin. The increase in AST level was inhibited by curcumin. These results suggest that curcumin and bisdemethoxycurcumin have potent hepatoprotective activities.

Korean Curcuma longa L. induces lipolysis and regulates leptin in adipocyte cells and rats

  • Song, Won-Yeong;Choi, Jeong-Hwa
    • Nutrition Research and Practice
    • /
    • v.10 no.5
    • /
    • pp.487-493
    • /
    • 2016
  • BACKGROUND/OBJECTIVES: Turmeric (Curcuma longa L.) has been reported to have many biological functions including anti-obesity. Leptin, peptide hormone produced by adipocytes and its concentration is increased in proportion to the amount of the adipocytes. In the present study, we examined the effects of Korean turmeric on the regulation of adiposity and leptin levels in 3T3-L1 adipocytes and rats fed a high-fat and high-cholesterol diet. MATERIALS/METHODS: Leptin secretion, free fatty acid and glycerol contents in 3T3-L1 adipocytes were measured after incubation of cells with turmeric for 24 hours. Rats were divided into four experimental groups: a normal diet group (N), a high-fat and high-cholesterol diet group (HF), a high-fat and high-cholesterol diet group supplemented with 2.5% turmeric extracts (TPA group) and a high-fat and high-cholesterol diet group supplemented with 5% turmeric extracts (TPB group). Serum samples were used for the measurement of leptin concentration. RESULTS: Contents of free fatty acid and glycerol showed concentration dependent increase in response to turmeric extracts. Effects of turmeric extracts on reduction of lipid accumulation in 3T3-L1 cells were examined by Oil Red O staining. Treatment with turmeric extracts resulted in increased expression levels of adipose triglyceride lipase and hormone-sensitive lipase mRNA. The concentration of leptin from 3T3-L1 adipocytes was significantly decreased by turmeric. Proportional abdominal and epididymal fats weights of the turmeric 5% supplemented group, TPB has significantly decreased compared to the HF group. The serum levels of leptin in the TPA and TPB groups were significantly lower than those of the HF group. CONCLUSIONS: Based on these results, we suggested that Korean turmeric may contribute to the decreasing of body fat and regulating leptin secretion.

Effects of Composts on the Growth, Yield and Effective Components of Turmeric (Curcuma longa L.) (울금의 생육, 수량 및 품질에 미치는 퇴비의 효과)

  • Han, Hyo-Shim;Woo, Seo;Kim, Dong-Kwan;Heo, Buk-Gu;Lee, Kyung-Dong
    • Korean Journal of Environmental Agriculture
    • /
    • v.29 no.2
    • /
    • pp.138-145
    • /
    • 2010
  • Curcuma longa L. (turmeric) is an important medicinal plant that has been historically used in herbal medicine and in the health food throughout Asia etc. Recently, the demands on rhizome of turmeric are increasing greatly by well-being boom, but there is not enough to meet the demands. To fulfill increasing demands, cultivation system strategies using the organic fertilizers are required to produce a greater amount of rhizome with good quality and yield. A field experiment was conducted to determine the effect of composts, NPK fertilizer(control, F), NPK + swine manure(SM) and NPK + fermentation manure from the wasted oriental medicine materials(OMWM), on rhizome yield and the content of bioactive components for quality. Our results showed that two compost applications can increase both rhizomes(24.1-25.9%) and curcumin(21.7-41.0%) yields, respectively, compared to F control. The content of amino acids increased significantly by SM and OMWM treatments. SM and OMWM application also increased the total phenol yields 7.8 and 8.7 g/10a compared with control 6.3 g/10a, the flavonoid yields 6.3 and 7.3 g/10a compared with control 5.3 g/10a, and also antioxidant activity 21.7 and 41%, respectively, as compared to the control. Especially, OMWM was more effective in total rhizomes yields and bioactivities and in the biosynthesis of curcumin and bioactive components than SM treatments, but the biological pathway was not clear, still. This experiment suggests that curcumin or bioactive components affected by adding SM and OMWM could increase the yields and quality of turmeric.

Evidence for the Participation of ATP-sensitive Potassium Channels in the Antinociceptive Effect of Curcumin

  • Paz-Campos, Marco Antonio De;Chavez-Pina, Aracely Evangelina;Ortiz, Mario I;Castaneda-Hernandez, Gilberto
    • The Korean Journal of Pain
    • /
    • v.25 no.4
    • /
    • pp.221-227
    • /
    • 2012
  • Background: It has been reported that curcumin, the main active compound of Curcuma longa, also known as turmeric, exhibits antinociceptive properties. The aim of this study was to examine the participation of ATP-sensitive potassium channels ($K_{ATP}$ channels) and, in particular, that of the L-arginine-nitric oxide-cyclic GMP-$K_{ATP}$ channel pathway, in the antinociceptive effect of curcumin. Methods: Pain was induced by the intraplantar injection of 1% formalin in the right hind paw of Wistar rats. Formalin-induced flinching behavior was interpreted as an expression of nociception. The antinociceptive effect of oral curcumin was explored in the presence and absence of local pretreatment with L-NAME, an inhibitor of nitric oxide synthase, ODQ, an inhibitor of soluble guanylyl cyclase, and glibenclamide, a blocker of $K_{ATP}$ channels. Results: Oral curcumin produced a dose-dependent antinociceptive effect in the 1% formalin test. Curcumin-induced antinociception was not altered by local L-NAME or ODQ, but was significantly impaired by glibenclamide. Conclusions: Our results confirm that curcumin is an effective antinociceptive agent. Curcumin-induced antinociception appears to involve the participation of $K_{ATP}$ channels at the peripheral level, as local injection of glibenclamide prevented its effect. Activation of $K_{ATP}$ channels, however, does not occur by activation of the L-arginine-nitric oxide-cGMP-$K_{ATP}$ channel pathway.

Effects of Planting Space on the Growth Characteristic and Curcumin Constituent of Curcuma longa L. (강황의 비닐하우스 재배가 생육 및 쿠르쿠민 함량에 미치는 영향)

  • Kang, Byoung-man;Yeo, Jun-hwan;Jung, Da-hwa;Kim, Young-guk;Chang, Jae-ki
    • Proceedings of the Plant Resources Society of Korea Conference
    • /
    • 2018.10a
    • /
    • pp.117-117
    • /
    • 2018
  • 강황(Curcuma longa L.)은 생강과(Zingiberaceae)에 속하는 다년생 아열대 초본 식물로, 약 40여 종이 존재한다고 알려져 있으며, 특유한 맛과 향기가 있어 세계적으로 널리 이용되고 있다. 현재 국내에는 전남 진도를 중심으로 경남, 제주 등으로 재배지역이 확대되고 있으며, 생산량이 증가하는 것으로 나타나고 있다. 한편, 강황은 우리나라에서 한약재와 식품원료로 구분하여 사용되고 있으며, 한약재 강황은 대한 민국약전을 근거로 규격품 한약재로서 품질관리가 이루어지고 있다. 특히, 국내산 강황은 대한민국약전의 지표성분 함량(쿠르쿠민, 데메톡시쿠르쿠민 및 비스데메톡시 쿠르쿠민의 합 3.2 % 이상)의 기준 규격에 미달되어 대부분 식품원료로 사용되고 있는 실정이다. 따라서, 본 연구는 강황의 비닐하우스 재배를 통해 한약재 활용 가능성을 알아보기 위해 수행되었다. 본 시험은 한약진흥재단 한약자원개발본부 시험포에서 수행되었으며, 강황 종근은 진도 재배 농가를 통해 수집되었다. 2017년 4월 20일에 일반 노지와 비닐하우스에 각각 정식하였다. 수확은 정식 후 200일에 각 실험구의 강황을 채취하여 초장, 엽수, 경경, 경수, 생체중, 건물중 등 생육 특성을 조사하였으며, 쿠르쿠민 함량 분석은 식품의약품안전처 고시법에 따라 HPLC를 이용하여 동시 분석 하였다. 하우스 재배가 강황의 생육에 미치는 영향을 살펴본 결과, 초장은 하우스 재배가 노지 재배에 비해 약 65% 높게 나타났으며, 엽수, 경경, 경수는 약 15~68% 높게 나타났다. 쿠르쿠민 함량은 하우스 재배가 노지 재배에 비해 약 53% 높게 나타났지만, 대한민국약전의 기준 규격에는 미달되었다. 따라서 본 연구 결과는 강황을 비닐하우스에서 재배할 때 생산성과 지표성분을 각각 68%, 53% 증수시켰지만, 한약재로 활용할 수 있는 기준 규격에는 못 미치는 것으로 나타났다. 향후, 강황 해외 지역종 수집 및 재배를 통하여 한약재 품질규격에 적합한 국내산 강황 재배기술 개발을 위한 기초자료로 활용이 가능할 것으로 사료된다.

  • PDF

Curcumin Induces Apoptosis and Inhibits Growth of Human Burkitt's Lymphoma in Xenograft Mouse Model

  • Li, Zai-xin;Ouyang, Ke-qing;Jiang, Xv;Wang, Dong;Hu, Yinghe
    • Molecules and Cells
    • /
    • v.27 no.3
    • /
    • pp.283-289
    • /
    • 2009
  • Curcumin, a natural compound extracted from rhizomes of curcuma Curcuma species, has been shown to possess potent anti-inflammatory, anti-tumor and anti-oxidative properties. However, the mechanism of action of the compound remains poorly understood. In this report, we have analyzed the effects of curcumin on the cell proliferation of Burkitt's lymphoma Raji cells. The results demonstrated that curcumin could effectively inhibit the growth of Raji cells in a dose- and time-dependent manner. Further studies indicated that curcumin treatment resulted in apoptosis of cells. Biochemical analysis showed that the expression of Bax, Bid and cytochrome C were up-regulated, while the expression of oncogene c-Myc was down regulated after curcumin treatment. Furthermore, poly (ADP-ribose) polymerase (PARP) cleavage was induced by the compound. Interestingly, the antiapoptotic Bcl-2 expression was not significantly changed in Raji cells after curcumin treatment. These results suggested that the mechanism of action of curcumin was to induce mitochondrial damage and therefore led to Raji cell apoptosis. We further investigated the in vivo effects of curcumin on the growth of xenograft tumors in nude mice. The results showed that curcumin could effectively inhibit tumor growth in the xenograft mouse model. The overall results showed that curcumin could suppress the growth of Burkitt's lymphoma cells in both in vitro and in vivo systems.

Demethoxycurcumin from Curcuma longa Rhizome Suppresses iNOS Induction in an in vitro Inflamed Human Intestinal Mucosa Model

  • Somchit, Mayura;Changtam, Chatchawan;Kimseng, Rungruedi;Utaipan, Tanyarath;Lertcanawanichakul, Monthon;Suksamrarn, Apichart;Chunglok, Warangkana
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.15 no.4
    • /
    • pp.1807-1810
    • /
    • 2014
  • Background: It is known that inducible nitric oxide synthase (iNOS)/nitric oxide (NO) plays an integral role during intestinal inflammation, an important factor for colon cancer development. Natural compounds from Curcuma longa L. (Zingiberaceae) have long been a potential source of bioactive materials with various beneficial biological functions. Among them, a major active curcuminoid, demethoxycurcumin (DMC) has been shown to possess anti-inflammatory properties in lipopolysaccharide (LPS)-activated macrophages or microglia cells. However, the role of DMC on iNOS expression and NO production in an in vitro inflamed human intestinal mucosa model has not yet been elucidated. This study concerned inhibitory effects on iNOS expression and NO production of DMC in inflamed human intestinal Caco-2 cells. An in vitro model was generated and inhibitory effects on NO production of DMC at 65 ${\mu}M$ for 24-96 h were assessed by monitoring nitrite levels. Expression of iNOS mRNA and protein was also investigated. DMC significantly decreased NO secretion by 35-41% in our inflamed cell model. Decrease in NO production by DMC was concomitant with down-regulation of iNOS at mRNA and protein levels compared to proinflammatory cytokine cocktail and LPS-treated controls. Mechanism of action of DMC may be partly due to its potent inhibition of the iNOS pathway. Our findings suggest that DMC may have potential as a therapeutic agent against inflammation-related diseases, especially in the gut.