• International Journal of Internet, Broadcasting and Communication
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• 제12권3호
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• pp.163-170
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• 2020

The purpose of this research is to organize panels centered on professional workers and experts in the sports media industry, identify structures and items of sports media commentators' attributes that conform to the present era through the collective agreement process, and evaluate the derived measures according to the scale development procedure. We would like to shed new light on the nature of commentators due to the rapid development of broadcasting technology and the emergence of new media platforms, the emergence and expansion of smart media receptors and the changing environment of modern sports broadcasting. For this purpose, a panel of experts from academia and industry related to sports management and sports media was organized and the attributes of commentators were analyzed using the Delphi method. Technology development in the sports market has created a new sports consumption environment. In these consumer environments, various environmental factors influence the consumer's consumption culture of sports consumers. For example, professional information, as well as interpretations of sports culture, history and society are becoming their share for sports commentators, and viewers are demanding more diverse characteristics from sports commentators. In this respect, the study of sports commentator attributes can be a deep understanding of the rapidly changing culture of sports consumption.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권4호
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• pp.274-277
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• 2004

Production of green fluorescent protein (GFP) as a model foreign protein using different culture scales under co-expression of Vitreoscilla hemoglobin (VHb) in the industrial Escherichia coli strain W3110 (a K12 derivative), was examined. It was found that the VHb co-expressing W3110, exhibited an exceptional and sustained production ability during cell cultures using different scales, while the VHb non-expressing strain showed variable production levels. This high and sustained production ability indicates that the VHb co-expressing E. coli W3110, could be successfully employed for practical large-scale production cultures without the need for serious consideration of scale-up problems.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1995년도 식물학심포지움 식물로부터 유용 2차대사산물의 생산 PRODUCTION OF USEFUL SECONDARY METABOLITES FROM PLANTS
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• pp.40-56
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• 1995

During the past two decades, China has seen her great progress in plant biotechnology. Since the Chinese market of herb medicine is huge, while the plant resources are shrinking, particular emphasis has been placed in plant tissue and cell cultures of medicinal plants, this includes fast propagation, protoplast isolation and regeneration, cell suspension cultures and large scale fermentation. To optimize culture conditions for producing secondary compounds in vitro, various media, additives and elicitors have been tested. Successful examples of large scale culture for the secondary metabolite biosynthesis are quite limited : Lithospermum ery throrhizon and Arnebia euchroma for shikonin derivatives, Panax ginseng, P. notoginseng, P. quinquefolium for saponins, and a few other medicinal plants. Recent development of genetic transformation systems of plant cells offered a new approach to in vitro production of secondary compounds. Hairy root induction and cultures, by using Ri-plasmid, have been reported from a number of medicinal plant species, such as Artemisia annua that produces little artemisinin in normal cultured cells, and from Glycyrrhiza uralensis. In the coming five years, Chinese scientists will continue their work on large scale cell cultures of a few of selected plant species, including Taxus spp. and A. annua, for the production of secondary metabolites with medicinal interests, one or two groups of scientists will be engaged in molecular cloning of the key enzymes in plant secondary metabolism.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2006년도 추계학술대회 논문집
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• pp.1115-1122
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• 2006

The railway design trends has been developed by the change of the society and culture through the consideration of the history of railway. The railway design trends is as the vehicles in the external form of railways, instantly means to plan and create the value added something like the form of the exterior design. The railway design has been changing form instinctive methods to settle the mass transport of the early days, to sensitive methods to pursue the railway culture centering around human being. As dividing by the style's change of railway in image scale aspects between 1800's and 2005 according to the life environment into the external nation, there are big differences so that is to study the development history of the railway design accordingly each nation (United Kingdom, Germany, France, Japan) and the style's change, to confirm into the design applied in society and culture needs, and to analyze by a year in changes of the environment and the railway design history. This paper analyzed railway introduced as railway models by top 4 nation (United Kingdom, Germany, France, Japan) in the past years(1800-2005) for their railway design trends by image scale positioning.

• KSBB Journal
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• 제22권2호
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• pp.91-96
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• 2007

본 연구에서는 음식폐기물 효소당화액을 BC생산 배지원으로 이용하여, flask정치배양과 50 L로 규모확대한 공기순환배양기에서 BC생산성을 검토하고, 생산된 BC의 제반물성을 조사하였다. 전단력을 낮추기 위해 당화액배지에 agar를 첨가하여 10 L, 50 L 공기순환배양기에서 배양한 결과, 50 L 규모로 scale-up한 반응기에서 5.64 g/L로 10 L 공기순환배양기 (5.84 g/L)에서와 동등한 BC 농도가 얻어져 BC 대량생산에 효과적인 배양방법임이 입증되었다. 50 L 규모로 확대된 공기순환배양기를 이용하는 본 배양 방법은 BC의 저비용 대량생산을 위한 효율적인 생산 방법 중 하나가 되리라 사료된다. 또한, 당화액을 배지로 하여 공기순환 방법으로 제조한 BC의 물성을 정치배양 방법으로 제조한 BC의 물성과 비교 검토한 결과, 공기순환법으로 제조하였을 때 중합도 및 역학적 성질이 약간 저하하였으나 결정성은 비슷하였으며, 배양기 용량의 대소에 따른 물성의 변화는 거의 없었다.

• 대한의생명과학회지
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• 제19권1호
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• pp.41-47
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• 2013

Pluripotent stem cells (PSCs) have lots of potential in biomedical sciences owing to its potential to differentiate into any kind of cells in the body. However, it is still a challenge to culture PSCs on a large scale for application to regenerative medicine. Herein, we introduce a synthetic polymer that enables large-scale suspension culture of human PSCs. By employing suspension culture, it became unnecessary to use conventional substrata such as mouse embryonic fibroblast (MEF) or Matrigel$^{TM}$, which are believed to be main causative sources of xenogeneic contamination in cultured human PSCs in vitro. Human PSCs were cultured in the medium in which elastin-like polypeptide (ELP) dissolved. The ELP in the medium became harden as temperature increases by transforming the medium into a semi-solid gel that supported growth of human PSCs in suspension. Gel-sol transition temperature of ELP can be adjusted by modifying the peptide sequence in which 5 amino acids, Val-Pro-Gly-Xaa-Gly, repeated sequentially. We constructed 3D suspension media having transition temperature around $33{\sim}35^{\circ}C$ using an ELP consisted of 40, 60, or 80 repeats of a monomer, which was Val-Pro-Gly-Val-Gly. Among the ELPs, ELP80 was chosen as the best ELP to support growth of human PSCs in suspension culture. This result suggests that the ELP80 can be a medium component for culturing human PSCs in large-scale.

• Child Health Nursing Research
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• 제17권4호
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• pp.222-229
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• 2011

Purpose: This study was conducted to determine the predictors of cultural sensitivity for nursing students in Korea. Methods: The participants in this study were 332 nursing students. Data collection was conducted through the use of questionnaires which were constructed to include a Cultural Sensitivity Scale, Knowledge of Culture, Interpersonal Reaction Index, and Self-efficacy Scale. Results: Results showed that cultural sensitivity of nursing students was middle range. Knowledge level of culture was very low. There was a significant positive correlation between cultural sensitivity and knowledge of culture, empathy, and self-efficacy. The significant factors influencing cultural sensitivity of nursing students were empathy, self-efficacy, experience of an overseas trip and awareness of cultural education. These four factors explained 23.8% of the variance in cultural sensitivity of nursing students. Conclusion: These results indicate that cultural sensitivity is an important link with empathy and self-efficacy in nursing students. For nursing students to enhance cultural sensitivity, a transcultural nursing curriculum needs to develop for appropriate and effective services in cross-cultural situations of the multicultural families in Korea.

• 한국미생물·생명공학회지
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• 제21권6호
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• pp.609-614
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• 1993

The optimum conditions for the submerged mycelial culture of Lentinus edodes SR-1 were elucidated to be incubation temperature of 25C, initial pH 4.0, agitation of 300 rpm, inoculation of 10.0%(v/v), and aeration of 1.0 v/v/m in TGY medium. The optimum c/n ratio and economic yield coeffcient for the submerged mycelial culture were 13.1:1 and 0.45 respectively. As the plant growth hormones test, SCM medium containing 0.5ppm of 2,4-dicholorophenoxyacetic acid increased mycelial yield in 1.1%, but 6-benzylaminopurine was not effective.

• 한국조경학회:학술대회논문집
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• 한국조경학회 2007년도 Journal of Landscape Architecture in Asia Vol.3
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• pp.157-161
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• 2007

Full-scale ferro-concrete building technology came was introduced in Japan in Meiji $35{\sim}40(1902{\sim}1907)$ and heralding the beginning of urban modernization. On the roofs of these new architectural constructions, full-scale rooftop gardens were also developed. We consider that gardens established on the roofs of hotel and department stores created a new, modernized garden culture, which greatly influenced the early modern urban culture of Japan, the drama of which it conceived based on the impression in a rooftop garden is made. In this paper, we discuss the influence of Meiji-Era cultural and technological advances on rooftop gardens constructed during the Taisho $Era(1912{\sim}1926)$, as represented by the gardens of Kobe's Oriental Hotel, Tokyo's Mitsukoshi Department Store and Shimonoseki City's Akita Company. Photographic and print sources are utilized to analyze the design features and temporal changes of these pioneering rooftop gardens, as well as their influence on urban culture.

• International Journal of Advanced Culture Technology
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• 제11권4호
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• pp.112-122
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• 2023

This study was conducted as part of a summer Korean language and culture program at a university in Korea, aimed at examining the changes in intercultural sensitivity among American students who participated in the program, as well as the Korean students who served as their language partners. The research utilized a mixed-method approach combining qualitative and quantitative analysis. To derive research findings, pre- and post-program Intercultural Sensitivity Scale surveys were administered to participating students and semi-structured interview data were collected from a randomly selected group of six students. The research findings indicated that among a total of twenty-seven students including both American and Korean students, significant changes in intercultural sensitivity were observed in the domain of Interaction Engagement. Furthermore, fourteen American students showed significant changes in their Interaction Confidence, while the Korean students who participated as language partners did not exhibit significant changes in the five subdomains of intercultural sensitivity.