• Reproductive and Developmental Biology
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• v.33 no.4
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• pp.203-209
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• 2009

The present study was performed to identify changes of plasminogen activator (PA) and plasminogen activator inhibitor (PAI) in porcine oviduct epithelial cells (POECs) during the estrous cycle. POECs obtained from ovary in pre-ovulatory (Pre-Ov), early to mid-luteal stage (Early-mid L) and post-ovulatory stage (Post-Ov). For the examine of PA activity, $1{\times}10^5$ fresh cells of POECs were cultured in DMEM/Ham F-12 containing 10% FBS and 0.2% amphotericin under humidified atmosphere of 5% $CO_2$ in air and $38^{\circ}C$. The urokinase-type PA (uPA) was observed at 7 days of POECs culture. PA activity was measured with culture prolonged of 0, 3, 6, 12 and 24 h after culture of 7 days. The PA activity were high significantly (p<0.05) at 12 h of culture, but PA activity were decreased with culture periods increased. The PA activity in POECs of Post-Ov stage were higher significantly (p<0.05) than that of Early-mid L and Pre-Ov stage. When PAI-1 and PAI-2 were added during the POECs culture, the PA were observed significant low activity (p<0.05). The PA activity and protein expression were decreased by PA inhibitor. This results suggest that PAI-1 and PAI-2 have a suppressive action on change of PA activity during the estrous cycle of pigs. Specifically, this study using PA inhibitor was effect the PA activity and PAI expression in oviduct epithelial cells in pigs.

• Proceedings of the Korean Society of Life Science Conference
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• 2005.09a
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• pp.36-36
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• 2005

• International Journal of Advanced Culture Technology
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• v.10 no.3
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• pp.1-10
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• 2022

This study examined the relationship between depression, anxiety, stress, and adaptation to college life of international students living in South Korea during the COVID-19 pandemic and the factors affecting the adaptation to college life. This study was carried out between December 3, 2021, to January 25, 2022, on international students living in South Korea. The questionnaires were composed of self-reported questionnaires, and the survey URL was sent as text messages to international students who understood the purpose and rationale of this study and consented to participate in the survey. The data were analyzed using SPSS WIN 22.0, and t-test, ANOVA, Pearson's Correlation Coefficient, and hierarchical regression were performed. As a result of the study, the average score of the study subjects was 8.44 points for depression, 8.28 points for anxiety, and 9. 28 points for stress. factors with significant differences in adaptation to college life according to general characteristics were living means and smoking. The relationship between the main variables, it was significant with depression (r=-.785, p<.001), anxiety (r=-.593, p<.001), and stress (r=-.726, p<.001). There was one negative correlation. It was found that the higher the depression, anxiety, and stress, the lower the college life adaptation. Lastly, depression (β=-.666, p<.001) was the factor affecting foreign students' adaptation to college life, and the explanatory power was 62%. Therefore, for international students to adapt to college life, it is necessary to establish an institutional strategy to detect depression, a negative psychological emotion, at an early stage and to systematically manage it. Also, it is necessary to find an intervention plan to relieve depression that can be applied in social isolation situations due to the spread of infectious diseases. Research confirming the intervention effect should be upgraded.

• Journal of the Korea Society of Computer and Information
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• v.22 no.12
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• pp.171-178
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• 2017

Investigations into family-friendly company culture have been carried out for various companies and conglomerates. But there has never been such investigation for police organization, so this investigation is intended to see the effect on organizational commitment and job performance of the organization culture that makes use of family-friendly program for the police organization. To this end, how the internal factors of family-friendly organization culture affects organization commitment and job performance is empirically analyzed with structural equation modeling. And it is found that family-friendly program has significant effect on organizational life and welfare, and the welfare, in return, is found to have significant effect on job performance.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2006.10a
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• pp.126-127
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• 2006

• Journal of Life Science
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• v.11 no.1
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• pp.7-10
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• 2001

Nonactin is the parent compound of a group of ionophore antibiotics, that known as the macrotetrolides. In previous report, in th course of screening superoxide radical-generating compounds from microbial sources, we first screened Streptomyces viridochromogenes JM-4151 that produces nonactin. It was proved that nonactin is superoxide radical-producing compound. In present study, we examined the optimal culture conditions of nonacin. Th optimal culture conditions for nonactin production were as follows: 1% soluble starch, 1% yeast extract, 0.2% ammonium nitrate, 0.06% magnesium sulfate, 0.2% calcium carbonate, initial pH 7.0 at 28$^{\circ}C$ for 96 h. The highest nonactin production was achieved in the production medium of initial pH7.0 at 28$^{\circ}C$ for 96h. The threshold level of dissolved oxygen was found to be above 33.2% at 28$^{\circ}C$ when 1% soluble starch was used as a carbon source. These results suggest that S. viridochromogenes JM-4151 might be a possible strain for industrial nonactin producer.

• Journal of the Korean Wood Science and Technology
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• v.35 no.5
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• pp.100-108
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• 2007

$\beta$-Glucosidase from Laetiporus sulphureus among the enzymes related to lignocellulosic biomass degradation to sugars for using alternative bioethanol production was characterized. The highest activity of $\beta$-glucosidase was obtained on cellobiose at shaking culture. For the characterization and purification of $\beta$-glucosidase culture solution was concentrated and then purified by FPLC using ion exchange and size exclusion column. According to the results of SDS-PAGE, native PAGE and microfluidic system of purified enzyme, protein band was observed at about 132 kDa. Optimal pH and temperature of purified $\beta$-glucosi-dase were 5.0 and $60^{\circ}C$, respectively. In the kinetic properties of $\beta$-glucosidase on various substrates such as sophorose, gentiobiose and cellobiose, $K_m$ was 0.81, 1.07 and 1.70 mM, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.3
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• pp.191-198
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• 2016

This study evaluated the effects of TMC (trace mineral-fortified microbial culture) supplementation on growth performance, carcass characteristics, and meat quality parameters of Hanwoo steers during the last 4 months of finishing period. The TMC was a combination of 0.4% trace minerals, 20.0% Na-bentonite, and 79.6% feedstuffs, which was inoculated with a mixed microbial culture (Enterobacter ludwigii, Bacillus cereus, B. subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae). Twenty-four steers were blocked by initial BW ($634{\pm}16kg$) and randomly allocated to one of two treatments (control vs. 3.3% TMC). The effect of TMC supplementation on the growth performance was not significant. There was no incidence of urolithiasis in TMC-fed steers. However 3 out 12 steers (25%) fed the control diet were observed to have urinary calculi. The carcass yield and meat quality parameters were not affected by TMC supplementation, however marbling score was increased in TMC-fed steers (P = 0.08). There was no effect of TMC treatment on the chemical composition of longissimus dorsi muscle (LM). The TMC supplementation increased concentrations of manganese (P < 0.01), cobalt (P = 0.02), iron, and copper (P = 0.06) in LM. In conclusion, TMC treatment did not negatively affect growth performance and meat quality parameters, and positively affected the trace minerals profile of LM.

• Current Research on Agriculture and Life Sciences
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• v.31 no.2
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• pp.94-100
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• 2013

An efficient plant regeneration protocol is developed for a standard-type chrysanthemum. When petal segments derived from flower buds (4 or 8cm in diameter) were used as the culture material, the highest shoot regeneration frequency (96%) was obtained on a Murashige and Skoog (MS) medium supplemented with 0.5 mg/L IAA, 2 mg/L BA, 3% sucrose, and a 0.8% agar. Pre-culturing the explants under dark conditions for 14 days produced better results for the shoot regeneration frequency than the explants cultured under a continuous 16 h photoperiod ($40{\mu}molm^{-2}s^{-1}$). The shoot regeneration frequency ranged from 19.0% for the Shinmato cultivar to 89.1% for the Baeksun cultivar. Activated charcoal (0.2%) enhanced the root formation of the regenerated shoots in a hormone-free MS medium. The rooted plantlets were acclimatized and successfully established in a greenhouse.

• The Journal of the Convergence on Culture Technology
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• v.7 no.1
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• pp.502-510
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• 2021

This study was identified to affection factors the life satisfaction among the college students with allergies by ecological approaches. This study was secondary analysis by using the 2015 Korean Community Health Survey (CHS) raw data, it analyzed 1,862 college students with allergies. Male students' life satisfaction were explained by 7.9% as degree of allergic diseases, frequency of breakfast, networking with friends, social activities with relatives, social activities with religion, satisfaction of natural environment and medical health service. Female students' life satisfaction were explained by 3.0% as frequency of breakfast meal, social activities with relatives and satisfaction of natural environment. We need to make the strategies and policies to improve of student's intake breakfast and make campus' green space.