• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.70.1-70
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• 2003

It was investigated whether culture filtrates produced by X. fastiduosa could be used to determine varietal susceptibility in grape cultivars to anthracnose as a substitute for pathogen inoculation or field screening. Bioassay of grape leaves with culture filtrates showed that their phytotoxicities were active and host-selective. Ethyl acetate extracts from those also showed the toxicities and host selectivity among grape cultivars. The sensitive range of plants to culture filtrates and their ethyl acetate extracts was consistent with the host range to the pathogen. Susceptible cultivars were sensitive to even highly diluted culture filtrates but resistant cultivars were not affected even at original culture filtrates. Susceptible cultivars were sensitive to the undiluted culture filtrates than highly diluted culture filtrates and the younger leaves were the more sensitive to the culture filtrates and their ethyl acetate extracts in grapes.

• 미생물학회지
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• 제12권3호
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• pp.101-114
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• 1974

The fine structure of HeLa cells treated with several mycotoxin-producing fungi (Aspergillus flavus ATCC 15517, Aspergillus parastiticus RIB 1037, Penicillium toxicarium RIB 4002, Penicillium cirinum SWU)238, Penicillium islandicum IFO 5235, Penicillium tadum IFO 5787 and Pencillium brunneum RIB 1172) has been examined and some details have been descried. The normal HeLa cell have numerous microvilli, large ovoid nucleus, pleomorphic mitochondria, electron-dense body, Golgi complex, mid-body and endoplasmic reticulum etc. Certain specific structural changes induced by culture filtrates of several mycotoxin-producing fungi have been noted. These alterations induced disappearance of Golgi complex, rER vacuolization, nucleolus attachment to the nuclear envelope nad appearance of certain vacuoles. There were not any changes by the treatment of culture filtrates of non-toxic fungi and only cell debris of some specimens can be observed by the injury of culture filtrates. The experimental animals treated with mycotoxin-producing fungi (Aspergillus flavus ATCC 15517, Aspergillus parasilicus RIB 1037, Penicillum citrinum SWU 238, Penicillium toxicarium RIB 4002, and Penicillium islandicum IFO 5235) were mal cells treated with culture filtrates.

• 한국응용곤충학회지
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• 제1권
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• pp.3-10
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• 1962

1) 강력한 위조독소(Wilt toxin)인 Fusaric acid를 생성하는 것으로 알려진 참깨$\cdot$시들음병균(Fusarium oxysporum f. vasinfectum)의 배양여액이 참깨의 종자발아 및 발아후의 유식물의 생육에 미치는 영향을 구명하기 위하여 이 시험연구에 착수하였다. 2) Fusarium oxysporum f. vasinfectum의 5종의 계통의 배양여액은 모두 참깨종자의 발아를 억제하고 종자의 흑변, 괴사를 초래한다. 3) 공시균의 어떤 계통의 배양여액에 대한 참깨종자의 발아반응에 있어서의 품종간 차이는 없다. 4) 공시균의 5종의 계통의 배양여액이 참깨종자의 발아를 억제하는 독성에는 뚜렷한 차이가 있다. 5) 공시균의 5종의 계통의 배양여액을 첨가한 육묘상에서는 발아후의 참깨유식물의 뿌리나 줄기의 신장이 다 같이 억제되며 조직은 흑변, 괴사하게 된다. 그러나 배양여액을 $10\%$ 첨가한 육묘상에서는 줄기의 신장생장 약간 촉진되는 경향이 있다. 6) 공시균의 배양여액을 $1\%$ 내지 $1\%$ 첨가한 Knop Solution에 본엽이 생기지 않은 참깨 유식물을 배양하면 참깨의 뿌리나 줄기의 신장생장이 억제되고 제2본엽이 전개할 때까지에 줄기나 잎자루나 연화, 흑변, 괴사하며 제1본엽에는 맥간에 염갈색반문이 형성되고 제2본엽은 권축, 흑변, 괴사하며 특히 제2본엽에 나타나는 병상은 포장에서 나타나는 참깨의 잎의 위조병징과 흡사하다. 7) 공시균의 5종의 계통간에 나타나는 배양여액의 독성의 차이는 공시균의 각계통자체의 기생에 의한 병원성의 차이와 일치하지 않는다

• Journal of Plant Biotechnology
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• 제44권4호
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• pp.394-400
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• 2017

포도나무 피어스병에 대한 품종간 저항성을 검정하는 데에 이용되는 병원균 접종법이나 포장저항성 검정법을 대체할 수 있는 방법으로 병원균(Xylella fastidiosa)이 생산하는 배양여액을 이용할 수 있는 검정법을 개발하고자 하였다. 상처를 가한 포도나무 잎에 병원균의 배양여액을 처리한 결과 독성이 발현되었으며 품종간에 차이가 나타났다. 병원균 배양여액의 에틸아세테이트 추출물은 공시한 포도나무에서 배양여액 처리와 동일한 반응을 유도하였다. 병원균 배양여액의 독성과 감수성은 에틸아세테이트 추출물과 동일하였다. 감수성 품종은 높은 비율로 희석된 배양여액에도 감수성을 나타내었으며, 저항성품종은 원액에서도 반응하지 않았다. 감수성 품종은 희석된 배양여액보다 원액에서 감수성이었으나 저항성 품종은 원액에도 저항성을 나타내었다. 본 연구에서 유럽종 포도 품종이 중도 감수성이었으나, 배양여액을 이용한 피어스병해 저항성 검정은 병해에 저항성인 유전자원 선발과, 교배조합의 저항성 실생의 조기에 선발에 중요한 기술로서, 향후 병해에 저항성인 포도품종 육성에 크게 기여할 것으로 여겨진다.

• Mycobiology
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• 제41권4호
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• pp.221-224
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• 2013

Aphids are one of the most destructive pests in crop production such as pepper, cucumber, and eggplants. The importance of entomopathogenic fungi as alternative pest control agents is increasing. Conidia of entomopathogenic fungi are influenced by environmental conditions, such as temperature and relative humidity, and cause slow and fluctuating mortality. These factors have prevented wider application and use of biocontrol agents. For investigation of means of mitigation of such problems, we conducted bioassays with 47 fungal culture filtrates in order to evaluate the potential of secondary metabolites produced by entomopathogenic fungi for use in aphid control. Among 47 culture filtrates cultured potato dextrose broth, filtrate of Beauveria bassiana Bb08 showed the highest mortality (78%) against green peach aphid three days after treatments. Filtrate of Bb08 cultured in Adamek's medium showed higher toxicity as 100% to third instar nymphs of the aphid compared with seven other filtrates cultured in different broths amended with colloidal chitin or oil. The culture filtrates and fungal cultures from media amended with colloidal chitin or oil had lower control efficacies than filtrates without these additives in three different media. These results indicate that the fungal culture fluid or culture filtrate of B. bassiana Bb08 cultured in Adamek's medium has potential for development as a mycopesticide for aphid control.

• 현장농수산연구지
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• 제14권1호
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• pp.85-92
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• 2012

곤포 사일리지에서 분리한 Penicillium, Aspergillus, Fusarium속 균의 곰팡이독소 생산 여부를 조사한 결과, Penicillium paneum 균주의 배양여액에서 citrin과 patulin이 검출되었고, Aspergillus flavus 균주의 배양여액에서는 아프라톡신이 검출되었으나 A. fumigatus가 생산하는 것으로 알려진 글리오톡신은 검출되지 않았다. Fusarium proliferatum 균주는 Fusarium속 균이 일반적으로 많이 생산하는 fumonisin, zearalenone, deoxynivalenol 등 곰팡이 독소가 검출되지 않았다.

• 한국미생물·생명공학회지
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• 제29권3호
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• pp.127-133
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• 2001

냉각탑수 중의 Legionella 속 균을 저감시킬 수있는 방법개발의 일환으로 L. pneumophila에 강한 항균성물질을 생산하는P. seruginosa KLP-2 균주를 냉각탑수로부터 분리 동정하였다 P.aeruginosa KLP-2 균주를 냉각탑수로부터 분리 동정하였다. P. aeruginosa KLP-2 균주의 배양여액은 L. pneumophila의 Vibro cholerae non-Ol Bacillus cereus, Bacillus subtilis 및 Staphylococcus aureus에 대해서도 항균성을 나타내었다. P. seruginosa KLP-2 균주 배양여액의 항균활성을 증가시키기 위한 최적조건은 0.1%의 $K_2$$HPO_4$와 0.05%의 $MgSO_4$$7H_2$O로 조성되 무기염배지에 탄소원으로써 1%의 glycerol, 질소원으로써, 0.6%의 proteose peptone이 첨가된 pH 7.0의 배지 조건에서 가장 높은 항균 활성을 나타내었으며 $35^{\circ}C$에서 20시간 진탕배양(50 rpm)한 후 4일간 정치 배양하였을 때 최대의 항균활성을 나타내었다.

• 한국버섯학회지
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• 제12권1호
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• pp.1-7
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• 2014

This study was initiated to investigate antioxidant, anti-acetylcholinesterase, and xanthine oxidase inhibitory activities and properties of fruiting bodies, mycelia, and fermentation culture filtrates from Phellinus igniarius. The contents of total phenols and flavonoid of fruit bodies, mycelia, and culture filtrate were 15.35-1.36 mg/g, 10.35-7.85 mg/g, and 8.25-5.36 mg/g. The 1,1-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging abilities of the extracts from the fruiting bodies, mycelia, and culture filtrates were 90.25-95.60%, 78.82-85.24%, and 76.32-82.50% at $50-400{\mu}g/mL$, respectively. The chelating ability of fruiting body extract on ferrous ions was higher than those of mycelia and culture filtrates tested. The anti-acetylcholinesterase inhibitory activity of the fruiting body extract at 400 ${mu}g/mg$ exhibited 91.10% on AChE, which is lower than that of positive control, galanthamine (94.82%). The xanthine oxidase inhibitory activities of the fruiting bodies, mycelia, and culture extract were 85.47%, 78.13%, and 72.49% at 400 ${\mu}g/mL$, respectively. Overall, the fruiting body extract has better anti-acetylcholinesterase, antioxidant and xanthine oxidase inhibitory activities than those from mycelia and culture filtrate.

• 식품보건융합연구
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• 제9권6호
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• pp.9-19
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• 2023

Lactobacillus plantarum and Leuconostoc mesenteroides are crucial functional starters and predominant isolates in a wide range of fermented foods, particularly kimchi, whose constituents exhibit bioactive properties. We previously developed a methodology using anion exchange resins to purify peptidyl compounds from Lb. plantarum LBP-K10. Antibacterial cultures of Lb. plantarum LBP-K10 were obtained from the respective cultures' supernatants and filtrates. However, conclusive evidence of the efficacy of kimchi filtrates in eradicating pathogenic bacteria is lacking. We aimed to simulate the potential effects of antibacterial filtrates that contained antibacterial compounds which were derived from cultures of Lb. plantarum LBP-K10. We acquired the kimchi filtrates using a combination of centrifugation and filtration methodologies, without the requirement for inoculation. The filtered liquid from Chinese cabbage kimchi, inoculated with Lb. plantarum LBP-K10 as a starter culture, and the non-inoculated liquid from Chinese cabbage kimchi (referred to as CCK and CCKRef, respectively) were were examined. CCK demonstrated greater inhibitory activity and a more significant bactericidal effect against the bacterial indicator strains. The minimum inhibitory concentration demonstrated comparable outcomes in tests against both Gram-positive and Gram-negative bacteria. This research offers a groundbreaking examination that displays the effectiveness of profiling peptidyl compounds within kimchi filtrates for curing bacterial infections.

• Asian-Australasian Journal of Animal Sciences
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• 제18권3호
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• pp.409-413
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• 2005

The objective of this research was to combine the physiological functionality of probiotics (Lactobacillus acidophilus and Bifidobacterium longum) and the milk-clotting activity of culture filtrate from lao-chao to develop a new dairy product which was different from the commercial yogurt. Rhizopus javanicus and Saccharomyces cerevisiae were chosen as a mold and yeast starter for production of culture filtrate. The study results indicated that both probiotic counts increased with incubation time and maintained $10^7$-$10^8$ CFU/ml after 6 h incubation with 10-30% culture filtrates. By contrast, samples with 40% culture filtrates inhibited the growth of L. acidophilus and B. longum. The more culture filtrates were added, the lower titratable acidities and higher pH values in Kou Woan Lao were detected after 36 h fermentation. No significant differences (p>0.05) were found for both L. acidophilus and B. longum, when grown in differing concentrations of skim milk powders. Storage results showed both L. acidophilus and B. longum exhibited excellent stability for 14 days at $4^{\circ}C$ in the Kou Woan Lao.