• Title/Summary/Keyword: culture content

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Quality Characteristics and Antioxidant Activities of Bread Added with Cedrela sinensis Powder (참죽 분말을 첨가한 식빵의 품질 특성 및 항산화 활성)

  • Kim, Min-A;Lee, Eun Ji;Jin, So-Yeon
    • Journal of the Korean Society of Food Culture
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    • v.29 no.1
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    • pp.111-118
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    • 2014
  • Cedrela sinensis is a Korean traditional wild herb found especially in the southern provinces of Korea. This study investigated the effects of Cedrela sinensis powder on the antioxidant activities and quality characteristics of bread. Bread was prepared with different amounts of Cedrela sinensis powder (at ratios of 0, 1, 2, and 3% of total flour quantity). The results showed that the dough pH, moisture, and fermentation tension levels decreased as Cedrela sinensis powder content increased. Bread volume and specific volume decreased (p<0.01) with an increasing amount of Cedrela sinensis powder (Bread weight increased (p<0.001)). As Cedrela sinensis powder content increased, color 'a' value decreased, and 'b' value increased. Sensory parameters such as color (p<0.01), flavor (p<0.01), appearance (p<0.001), taste, softness (p<0.05), and overall quality (p<0.01) of bread containing 2% Cedrela sinensis powder were measured. Total phenol contents and DPPH free radical scavenging activity of bread significantly increased with increasing Cedrela sinensis powder content (p<0.001). Based on these results, we suggest that Cedrela sinensis can be used for increasing the consumer acceptability and functionality of bread.

Effect of Xylan on Production of Xylanolytic Activity from Penicillium verruculosum (Penicillium verruculosum의 Xylan분해활성도의 생성에 대한 Xylan의 영향)

  • 조남철;정두례;유영균
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.4
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    • pp.423-427
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    • 1992
  • During the cultivation of Penicillium verruculosum in the medium containing xylan as a sole carbon source for 26 days, xylanolytic activity and some changes were investigated. Protein content and xylanolytic activity, p-Nitrophenyl-$\beta$-D-xylopyranoside (PNPX), p-Nitrophenyl-$\beta$ -D-glucopyranoside (PNPG) hydrolytic activities were increased until 8 days but reducing sugar content was not correlated to protein content. When crude proteins from the culture broth were separated on SDS-PAGE, distribution of proteins was different from the culture broth of cellobiose octaacetate (COA) medium. The culture broth of xylan medium had high hydrolytic activity on xylan but not on cellulose. Furthermore, xylanolytic products were showed xylose, xylobiose and oligosaccharides on thin layer chromatography, and xylobiose was major product. Those result suggested that xylanolytic activity of culture broth was endo-type hydrolysis. Optimum temperatures of xylanolytic activity and PNPX hydrolytic activity of culture broth were 50~6$0^{\circ}C$ and 60~7$0^{\circ}C$, respectively and optimum pHs were 3.0~4.0 and 4.0~5.0, respectively.

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A Study on the Jewelry decorative pattern based on Wa-Dang in Unified Silla period (통일신라시대 와당을 모티브로 한 주얼리장식용 문양 연구)

  • kyeng-Tae Kim
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.6
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    • pp.113-122
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    • 2023
  • This study was conducted on the premise of the development of cultural products using relic assets of traditional culture in a knowledge and information society led by culture and soft power. It was conducted in the context of exploring the possibility of cultural content products of Wa-Dang relics excavated from traditional architecture in the Unified Silla Period and expanding the scalability of commercialization motifs that are highly useful in jewelry design. First, the original form, material, use, size, meaning, and formative aesthetics of Wa-Dang were identified through literature and media research. Among the considered Wa-Dang, 10 types of Wa-Dang which represent the category and have values in modules and patterns were selected, and, then, circular images were extracted and modularized with a "formal simplification technique." Based on the "mathematical symmetry analysis technique," which is a method of systematizing pattern composition arrangement format. we derived a planar formative element that can be used in the development of the cultural content industry and jewelry design. In order to expand its usability in the jewelry industry in the future, it was presented as a 2D digital image. In the future, we hope more studies on the various cultural content industry utilizing the traditional culture will be carried out.

Mineral Absorption by Cymbidium Jungfrau in the Solution Culture (서양 심비디움 양액재배에서의 무기물 흡수)

  • Song, Sung-Jun;Boo, Chang-Ho;U, Zang-Kual
    • Korean Journal of Environmental Agriculture
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    • v.18 no.1
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    • pp.35-40
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    • 1999
  • $N(^{15}N)$ and $P(^{32}P)$ absorption by 2 year-old Cymbidium Jungfrau in solution culture were investigated. Growth, photosynthesis rate, chlorophyll content and mineral composition of Cymbidium in the solution culture with bark or granular rockwool were compared with these parameters in the conventional pot culture. Nitrogen absorption by Cymbidium was higher in full sunlight than in 60% of sunlight while P absorption was higher in 60% of sunlight. Sixty seven % of N absorbed in plant was redistributed to the bulb(39%) and leaves(28%) while 46% of P absorbed was fund in the bulb (36.2%) and leaves (10.2%). Accumulation of P in leaves was 3-fold lower than that of N. N and P absorption in 0.5 or 1 year- old daughter plant Bowing vigorously were greater than in immature daughter or mother plant. The absorption rate of phosphorus in Cymbidium was 350-fold lower than that of barley. Greater shoot length and bulb diameter, and higher fresh weight, photosynthesis rate and chloroployll content were observed in the solution culture than in the conventional pot culture. Solution culture had-also more content of N, P, K and Mg in leaves, bulb and root than conventional pot culture but did not that of Ca. A large part of the nutrient absorption was occurred during vegetative growth. Also, There was no difference between bark and rockwool in the solution culture due to the improvement of poor dispersion of nutrient solution in bark.

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Production of red pigments by Monascus purpureus in solid-state culture

  • Park, Hae-Yeon;Lee, Beom-Gyu;Jeong, Uk-Jin
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.315-316
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    • 2001
  • In this study various nutritional and environmental parameters such as, initial moisture content, pH. inoculum size, air rate, sample size and nutrient supplement that influence pigment production were evaluated in solid-state cultures. optimum initial moisture content and pH were determined to be 50% and 6.0, respectively. The supplement of the substrate with different carbon, nitrogen, and mineral source reveals a more inhibitive effect as the substrate concentration increase. optimum aeration rate was determined to be 2vvm in flask culture. The maximum amount of red pigment, 3500 OD/g dried fermented rice, was obtained in optimum conditions which is obtained in solid flask culture.

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Plasmid Stability and Cloned-Gene Expression in Continuous Culture of Recombinant Escherichia Coli Under Derepressed Condition

  • Nam, Soo-Wan;Kim, Byung-Kwan;Kim, Jung-Hoe
    • Journal of Microbiology and Biotechnology
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    • v.4 no.1
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    • pp.1-6
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    • 1994
  • Continuous culture was carried out with a recombinant Escherichia coli W3110/pCR185, which encodes trp-operon enzymes when the temperature is shifted from $37^{circ}C\;t;42^{\circ}C$. Under derepressed condition of $42^{\circ}C$. plasmlid stability and gene expression were analysed as function of the dilution rate. The stability of plasmid increased with the dilution rate, but maximal levels of gene expression (tryptophan concentration) and plasmid DNA content were obtained at the lowest dilution rate, $0.075\;hr^{-1}$. The plasmid instability, observed at low dilution rates, could be explained by the unbalanced biosynthetic state of the recombinant cell harboring a high copy number of plasmid.

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Selection of mutant Phaffia rhodozyma and Determination of Optimum Culture Conditions for Astaxanthin Production (Astaxanthin 생산을 위한 Phaffia rhodozyma의 변이균주 선발과 최적 배양조건 결정)

  • 유성선;유연우
    • Microbiology and Biotechnology Letters
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    • v.29 no.2
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    • pp.96-103
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    • 2001
  • Phaffia rhodozyma is the most promising microbial source of astaxanthin production, though wild-type strains are needed to increase the astaxanthin content for commercial production. To increase astaxanthin content for commercial production, a mutant strain of P. rhodozyma was selected and culture conditions of the mutant selected were optimized. P. rhodozyma was treated with mutagenic agent such as NTG, acriflavine, and UV in serial order and carotenoids hyper-producing mutant strain was selected based on the capabilities of cell growth on the agar plate containing chemical inhibitors and carotenoids production. Among the mutants tested, a mutant WS-2 was finally selected. Mutant WS-2 produced 1.26mg carotenoids/g-dry cell weight and this value was about- 4-folds higher than that of wild-type. The optimum culture conditions were $24^{\circ}C$ of temperature, 1.5vvm of aeration and 300rpm of agitation. In the optimized condition, cell and carotenoids concentrations were 7.62g/l and 14.9mg/l, respectively.

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A Content Analysis of Traditional Wedding Artifacts (혼례유물에 대한 내용분석)

  • 최배영
    • Journal of Families and Better Life
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    • v.21 no.5
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    • pp.155-170
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    • 2003
  • This paper will provide a basic analysis of traditional wedding artifacts as they appear on museum websites and their value for scientific research. The results of this study are as follows: 1. There were a total of 115 wedding artifacts(72 written documents related to weddings, 43 marital items) on the website of a museum. Most of them were related to the latter half of Cho-sun Dynasty. From these artifacts, we may understand the culture surrounding wedding during the transitional period from the Cho-sun Dynasty to modem time. 2. By analyzing such wedding artifacts, we can supplement existing methods of study such as literature research and case histories. In this way, we may better understand the history and culture of weddings. 3. In order to gain a deeper understanding of the culture of weddings we need to adopt a more interdisciplinary approach of wedding artifacts.

Growth and Astaxanthin Production of Phaffia rhodozyma AJ-6 by Fed-batch Culture

  • Kim, Su-Jin;Yu, Yeon-U
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.301-304
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    • 2000
  • Fed-batch culture was designed to increase cell concentration and astaxanthin content by mutant AJ-6 of Phaffia rhodozyma. Fed-batch culture was performed in the continuous feeding with manual adjustment of flow rate to control glucose concentration. When the final glucose concentration was 100 g/L, the cell and astaxanthin were 38.3 g/L, 34.8 mg/L, respectively. Addition of ethanol(10 g/L), when glucose was depleted, the cell and astaxanthin concentration were 37.2 g/L and 45.6, respectively, 5 g/L of acetic acid supplied, 40.6 g/L, 43.9 mg/L were obtained. Ethanol and acetic acid enhanced the astaxanthin content act as precursor of carotenoid synthesis.

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Production of Red Pigments by Monascus purpureus in Solid-state Culture

  • Lee Bum-Kyu;Piao Hai Yan;Chung Wook-Jin
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.1
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    • pp.21-25
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    • 2002
  • To maximize and sustain the productivity of Monascus pigments, various environmental and nutritional parameters, such as the initial moisture content, pH, inoculum size, sample size, and nutrient supplement, that influence pigment production were evaluated in solid-state cultures as follows: initial moisture content, $50\%;$ pH, 6.0; inoculum size $1\;\times\;10^4$ spore cells $(grams\;of\;dry\;solid\;substrate)^{-1};$ sample size, 300 g. All supplementary nutrients (carbon, nitrogen, and mineral sources) added has inhibitory effects on the cell growth and red pigment production. In open tray culture the maximum biomass yield and specific productivity of red pigments were 223 mg DCW $(grams\;of\;initial\;dry\;substrate)^{-1}$ and, $47.6\;OD_{500}\;(DCW\;grams)^{-1}h^h{-1}$ respectively.