• Journal of Plant Biology
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• 제11권1호
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• pp.15-21
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• 1968

Chlorella ellipsoidea were grown in a Mg-free medium. Aliquots of the algal cell were taken out at the beginning and predetermined time intervals during the culture and were analyzed the contents of phosphate in various fractions of the cell constituents. The results obtained were compared with those of the control. When Chlorella cells were grown in a Mg-free medium, the contents of phosphate in the DNA protein, RNA-olyphosphate complex, nucleotidic-lbileP, and PCA-soluble, fractions decreased compared with those of the control, while the content of acid insoluble polyphosphate increased significantly. On the otherhand, RNA-P and lipid-P showed the tendency of decrease at the early stage of the culture, but they were increased more than those in the control as culture proceeds. It is showed that phosphate turnover from acid-insoluble polyphosphate into DNA, protein, and RNA-polyphosphate complex was inhibited by magnesium-deficiency of the cells.

• Tuberculosis and Respiratory Diseases
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• 제67권5호
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• pp.395-401
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• 2009

As the prevalence of tuberculosis declines, the proportion of nontuberculous mycobacterial (NTM) lung disease is increasing in Korea. The combined use of liquid and solid media increases the sensitivity of mycobacterial culture and shortens culture time. Because NTMs are ubiquitous in the environment, NTM lung disease requires strict diagnostic criteria to prevent over-diagnosis of NTM lung disease. Mycobacterium avium complex is the most common pathogen of NTM lung disease in Korea and present in two forms: upper lobe cavitary and nodular bronchiectatic form. Decision of treatment of NTM lung disease depends on the infecting species and overall condition of the patient. Because medical therapy requires the use of multiple drugs over 18 to 24 months, surgery for localized disease may be useful for those species refractory to medical therapy.

• 한국멀티미디어학회논문지
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• 제25권10호
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• pp.1457-1467
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• 2022

The relationship between film and culture is established through a shared focus on people's lives. Culture changes at different times, which makes a dynamic and complex relationship between film and culture. Understanding this relationship helps to provide better support for current filmmaking. The purpose of this study is to provide insights into the macro issue of film and culture by exploring the relationship between film theme and culture concept. To this end, we used a case study to explore the connection between themes and culture concepts in Disney's Mulan (1998, 2020) through literature and analyses of culture concepts (Chinese "imagery" and Postmodernism). The study concludes that, "Culture assemblage" and "Vision is king" have become cultural trends that influence the creation of film theme and style to a certain extent; how to balance the relationship between the core idea of film theme and the external forms is an important issue in current filmmaking. The article contributes to promote thinking about culture in film creation, and its research perspective on different cultures and histories is innovative.

• 디지털융복합연구
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• 제15권12호
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• pp.469-475
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• 2017

논고는 복합문화적인 측면에서 바라 본 현대도시와 공공디자인의 특징 등에 대한 것이다. 본 연구는 현대도시와 문화에 대한 연구를 위해 관련 문헌 조사 및 다양한 이론적 고찰 등 실증주의적 연구 방법을 적용하였다. 본 연구의 목적은 문화와 복합문화에 대한 논의와 그에 따라 현대도시와 공공디자인과의 관계성을 모색하기 위한 것이다. 이에 따라 본 연구에서는 우리나라의 대표적 복합문화 공간으로서 스타필드 코엑스 사례를 분석하고 이를 공공디자인의 개념으로 재해석하였다. 덧붙여 현대도시가 가진 문화적 혼종성과 복합성의 성격을 공공공간에 융합하여 공공시설물에서의 사적공간의 필요성을 제언하였다. 하지만 본 연구는 우리나라의 복합문화의 개념 정의에 있어서 이론적 논의는 있었으나 이를 뒷받침 할 수 있는 실증적 사례를 제시하지 못하는 한계점을 가지고 있다. 이는 후속연구인 현대도시의 복합문화공간에 관한 고찰을 통해 실제 사례를 조사 연구를 통해 보완하도록 하겠다.

• 한국주거학회:학술대회논문집
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• 한국주거학회 2009년도 추계학술발표대회 논문집
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• pp.245-250
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• 2009

A variety of change in modern society has affected a family structure. In addition birth rate is getting lower and nuclear family type is getting common, influence of children has highly increased. According to this phenomenon, needs and concern about education and culture for children increase sharply. Children cultural complex can be learning field, which helps children not only to rediscover the world by themselves but also to have various and abundant experience. The purpose of this study is going to extract a planning factor of the children cultural complex through case studies of korean and oversea project for adaptable children cultural complex. In addition, this study tries to analyze the features of the children cultural complex in korean and oversea. Consequently, The analysis result is to be introduced party room to children cultural complex, used the PVC tile and carpet tile for safety of children and variety, and executed program relevant to nature.

• 한국미생물·생명공학회지
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• 제27권4호
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• pp.327-332
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• 1999

A highly productive fed-batch fermentation process was developed for the production of L-ornithine by using a new stabilized strain, Breviabcterium ketoglutamicum BK52. Fed-batch cultures with a continuous feeding of the complex medium were conducted on various operating conditions. The optimal concentration of phosphate in the complex medium was 2.1g/L. The optimal feeding rate of L-arginine was 0.028g/L/hr. The optimal feeding point of the complex medium was determined to be at 40 OD of the cell mass. The final L-ornithine concentrations within 64hrs of cultivation in 5 and 50 liter fermenters were 73g/L and 71g/L, respectively. The maximum overall L-ornithine productivity was 1.14g/L/hr which was about 2 times higher than that of the conventional fed-batch culture with intermittent feeding. The overall productivity of the fermentation system is remarkably improved by employing the optimized conditions, and it offers a significant potential for industrial application.

• 식물조직배양학회지
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• 제21권1호
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• pp.59-63
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• 1994

세포유전적 유형이 다른 무릇 (Scilla scilloidise Coplex)의 조직배양에서 유도된 캘러스에서 염색체 변이를 조사하였다. AA유형의 캘러스에서 심한 수적, 구조적 변이가 관찰된 반면, BB 유형의 캘러스 세포에서는 상염색체의 변이가 발견되지 않았다. 이질 4배체인 AABB유형에서는 두개의 hypoploid 세포만 관찰되었으나, B게놈의 진정4배체인 BBBB유형에서는 hypoploid 세포와 함께 hyperploid 세포가 관찰되었다. 캘러스 세포에서 상염색체의 안정성은 B염색체를 지닌 식물에서 유도된 캘러스에서 더 높게 나타났다. 이는 배양세포에서 B염색체가 상염색체의 안정성에 선택적으로 기능하기 때문인 것으로 여겨진다.

• 한국실내디자인학회논문집
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• 제22권5호
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• pp.68-77
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• 2013

'The Third space,' which is the concept that embraces sociological, spatial, and marketing concepts, refers to the place which is other than the home and the workplace and which is a comfortable frequented place where meditation, psychological relaxation, and social needs can be satisfied. 'The Third space' has the characteristics of comfort, openness, interactivity, playfulness, and diversity. In modern times, most of consumption spaces play the role of 'the Third space,' and the typical example is $Caf{\acute{e}}$. $Caf{\acute{e}}$ began from its origin with the characteristics of 'the Third space,' and recently it has become the complex cultural space of urban dwellers. $Caf{\acute{e}}$ as 'the Third space' has the characteristics of a healing space, communication space, personalized space, value-expressive space, culture and consumption space, nomad space. Such characteristics of $Caf{\acute{e}}$ are well represented in the cafes around the regions where 'street culture' has recently been formed. As examined through the examples, $Caf{\acute{e}}$ as 'the Third space' can be more strategically produced by establishing themes through storytelling, giving spatial identity through style creation, diversifying the sequence through complex space organization, and establishing interaction devices through experience elements.

• 한국가축번식학회지
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• 제17권2호
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• pp.75-83
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• 1993

Purine has been identified in the preparation of follicular fluid and shown an activity in maintaining oocyte meiotic arrest. Therefore this study was performed to examine the inhibitory effect of purine on germinal vesicle break down(GVBD) in the presence and absence of human fetal cord serum(HFCS) or human mature follicular fluid(HMFF), as a protein source, in vitro culture. Immature oocytes(GV stage) were collected from ovaries of 21∼28 days old ICR mice by puncturing the antral follicles with a fine needle, at 48 hrs after PMSG injection. Some of the oocytes were denuded by drawing the cumulus-enclosed(complex) oocytes in and out of a pasteur pipet. Complex oocytes and denuded oocytes were cultured 3 hrs. in T6 media containing 0.75mM adenosine or/and 4mM hypoxanthine, with HFCS or HMFF. Their GVBD rates were observed at every 1 hr. during the culture time. Both adenosine and hypoxanthine have shown a time-dependent inhibitory effect on GVBD in complex and denuded oocytes and the inhibitory effect was maximized in culture medium containing hypoxanthine and adenosine. HFCS and HMFF increased the GVBD rates in the presence of the purines, thus HFCS and HMFF may contain a factor that could reverse the inhibitory effect of purines. Also complex oocytes were more sensitive to not only the inhibitory effect of purines but the promoting action of HMFF on GVBD than denuded oocytes. Therefore it was reconfirmed that granulosa cells play an important part in meiotic arrest and resumption.

• 한국공간구조학회지
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• 제13권2호
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• pp.18-24
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• 2013