• 보존과학연구
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• 통권30호
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• pp.157-170
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• 2009

We have evaluated the antifungal activities of oak vinegar to develop a natural biocide for organic cultural heritage. Fungi used in this study were screened from the cultural heritages, Kyujanggak and JanggyeongPanjeon and tested on organic substrates-degrading ability. In the results, 7 species of fungi have produced the extracellular enzymes to degrade CMC, xylan, lignin. Thus, we have used these seven species fungi to investigate the antifungal activity of oak vinegar in this study. In the result, the antifungal activity of oak vinegar indicated positive potencial. Especially, methylene chloride and ethylacetate fractions of the oak vinegar had high activities at the concentration of 5.0mg/disc. In these fractions, many different kinds of compounds such as phenolic and furfural, etc. were analyzed by GC-MS. The experiments indicated that the development of a biocide using natural extracts can have a potential to conserve of organic cultural heritages.

• 보존과학연구
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• 통권26호
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• pp.5-25
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• 2005

A consideration number of investigation have begun to elucidate the essential role biological agents play in the deterioration of stone. What is becoming clear is that many factors affect the durability of stone. Physical, chemical, and biological agentsact in co-association, ranging from synergistic to antagonistic, to deteriorate stone. Biodeterioration has usually been considered to be a degradation process following the initial deterioration effects of inorganic agents, especially objects of cultural value such as pagoda, stature of Buddha etc. These agents were thought to condition stone surfaces for microbial contamination due to structural changes and enrichment of inorganic organic nutrient substrates. This report concentrates on the action of biodeteriogens from bacteria to algae and higher plants. Preventive and remedial methods are surveyed, as are a selection of chemical treatments.

• 보존과학회지
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• 제29권1호
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• pp.1-14
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• 2013

홍지문 오간수문은 홍지문에 부속된 홍예 수문이다. 이들의 주요 구성암석은 흑운모화강암, 홍장석화강암, 화강섬록암으로서 서울 인근에 분포하는 화강암류와 암석기재적 특징이 매우 유사하다. 오간수문에 나타난 손상현상은 주로 백색, 흑색 및 황색 변색이며 백화현상이 가장 심각하다. 5기의 홍예 수문에서 공통적으로 측벽의 변색이 천장보다 심하고, 천장에서는 종유석이 성장하고 있다. 오염물 분석 결과, 백화현상을 야기하는 물질은 다양한 형태를 보이는 방해석으로 판명되었다. 보존처리는 오간수문 전면에 대한 건식세정과 표면에 피각되어 있는 백화물질을 제거하기 위해 부분적으로 고압세척 및 백화제거제 등을 사용하였다. 현재는 일차적인 보존처리가 완료되어 양호한 상태를 보이고 있으나 향후 오간수문의 훼손을 저감하기 위해서는 수문 상부에 배수로를 확보하여 수분의 이동과 배출을 제어하는 것이 필요하다.

• Plant Resources
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• 제3권3호
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• pp.226-232
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• 2000

The availability of enokitake cultural waste for Lentinus edodes cultivation was investigated, although hardwood sawdust has traditionally been used as a substrate for this fungus. Firstly, physiochemical characteristics of cultural waste were analysed. Secondly, mycelial growth characteristics and fruiting yields of L. edodes on waste treated in some methods were determined. Physiochemical characteristics of enokitake cultural waste showed that the millwaste complex was a little degraded by enokitake fungus and suggested the probability that most component lost by enokitake could be rice bran. Mycelia of L. edodes grew and fruited well on waste supplemented by fresh rice bran and Quercus sawdust although didn't on waste only. Mycelial growths of these fungi on waste were accelerated when supplemented by rice bran to the percent of 40(w/w) but decreased or suppressed at above ratios(30, 40%, w/w). Supplementations of oak sawdust at above 40%(w/w) of the waste and rice bran at 20%(w/w) of the sawdust allowed such a good mycelial growth as to be selected as a pertinent mixing ratio for fruiting medium. A fruiting yield on enokitake cultural waste supplemented by oak sawdust (at 40% of the waste, w/w) and rice bran (at 20% of the sawdust, w/w) was not inferior to that on oak sawdust supplemented by rice bran only (at 20% of the sawdust, w/w). These results indicated strongly the potentiality of enokitake cultural waste as raw materials for shiitake cultivating substrates.

• Mycobiology
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• 제31권4호
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• pp.200-204
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• 2003

Cultural characteristics and phylogenic relationships were investigated and classified among collected strains in Pholiota spp. which contain P. adiposa, P. squarrosa, P. nameko etc. They were tested on the four different media(PDA, MCM, YM, MEA) and sawdust(Alder, Oak, Pine, Popular) substrates. There was a little variation according to the media and sawdust substrates, although PDA and popular sawdust substrate seemed to be better. Most strains showed white colonies, but some strains were brown. Mycelial growth length differed according to the strains. To classify species, the internal transcribed spacer regions(ITS) of the ribosomal DNA(rDNA) repeats from Pholiota spp. were amplified using polymerase chain reaction(PCR) and then sequenced. According to the analysis of ITS sequences, they were classified into five clusters. Their spacer regions were $644{\sim}700$ nucleotides in length. The reciprocal homologies of each ITS region among these strains were ranged from $49.6{\sim}99.9%$. The phylogenic analysis might give a criterion to classify species in the collected strains.

• 생물환경조절학회지
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• 제4권2호
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• pp.159-166
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• 1995

본 실험은 배수성과 통기성이 우수한 양액 재배 배지로써 perlite와 구입이 용이한 왕겨 및 훈탄의 혼합배지를 이용하여 스티로폼 성형베드 및 상자, 플라스틱 자루, 폿트 등의 재배용기의 차이와 배지의 종류에 따른 양액재배 오이의 생육 및 수량반응을 구명하고자 수행하였 다. 1. 스티로폼베드와 상자가 다른 용기보다 초장이 높게 나타났으며, 스티로폼 상자를 용기로 사용할 때 펄라이트 단용처리구가 275.5cm, 왕겨 혼합처리구가 277.0cm로 초장이 가장 높았다. 2. 엽면적은 스티로폼 성형베드와 상자가 가장 높게 나타났고, 자루재배, 폿트재배의 순으로 나타났다. 스티로폼 성형베드와 상자의 경우에는 펄라이트 단용>왕겨 혼합배지>훈탄 혼합배지의 순으로 나타났으나, 자루재배에서는 왕겨, 폿트재배에서는 왕겨 혼합배지에서 엽면적이 높게 나타났다. 3. 과실수량은 스티로폼 상자를 용기로 한 펄라이트 단용구가 2097.1g으로 가장 많았으며 과수도 13.4개로 높게 나타났다. 4. 상품과율은 스티로폼 베드와 상자에서 70%이상을 나타냈으나 자루재배와 폿트재배에서는 70%이하로 나타났다. 5. 곡과율과 선세과는 자루재배에서 10%이상으로 높게 나타난 반면 스티로폼 상자에서는 낮게 나타났다.

• 펄프종이기술
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• 제42권2호
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• pp.20-26
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• 2010

Paper composed mainly of organic materials and they can be used as nutrient by microorganisms. Therefore microorganisms are damaging the important documents and works of art irreversibly. Previous research reported that mycelium or spores of the fungi were observed on the wax of the volumes of King Sejong especially were heavily deteriorated by fungi. Fungal strains isolated from the annals of Joseon dynasty were identified as Biscogniauxia atropunctata, Aspergillus versicolor, Penicillium polonicum, Ceriporia lacerata, Irpex lacteus. Especially Aspergillus and Penicillium are able to grow on the substrates having a 7-8% moisture content, which is much lower than the general storage condition (RH55-60%) of the paper cultural properties. Moreover, they are known as fungi cause paper deterioration and discoloration. In this study, we selected Aspergillus versicolor and Penicillium polonicum among five strains, then biological aging has been executed for 30 days. The growth of Aspergillus versicolor and Penicillium polonicum in wax was lower than wax treated paper and Hanji. The growth of them in beeswax treated paper and Hanji both were excellent.

• 한국균학회지
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• 제27권4호통권91호
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• pp.252-255
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• 1999

노루궁뎅이버섯균의 균사생장과 밀도에 가장 적절한 종균배양적 특성을 요약하면 적정온도는 $25^{\circ}C$, pH는 5, 톱밥 배지의 적정 수분함량은 $65{\sim}70%$이며, 주재료는 참나무톱밥, 첨가재료는 밀기울이었다. 또한 적정 첨가재료인 밀기울의 첨가량은 20%, calcium carbonate의 첨가함량은 0.1%이며, magnesium sulfate의 첨가함량은 0.2%, sucrose의 첨가함량은 0.2%이었다.

• 한국식품영양과학회지
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• 제19권3호
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• pp.201-206
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• 1990

The optimal cultural conditions for production of the bluish purple prgment by the cultivation of Streptomyces californicus KS-89 were determined with various substrates. The carbon and nitrogen sources on the production of pigment indicated that soluble strarch and glycerol as carbon sources and sodium glutamate sodium nitrate as nitrogen sources given a mzimum yield of the pigment at 3$0^{\circ}C$ for 7 days. The addition of ferrous sulfate was essential. The highest production of pigment was observed with cultivation in a medium containing 2.0% soluble starch 1% glycerol 0.5% sodium glutamate 0.05% sodim nitrate 0.001% L-proline 0.025% K2HPO4 0.005% MgSO4 .7H2O, 0.04% FeSO4.7H2O, 0.001% thiamine.HCl and pH7.0.

• 한국버섯학회지
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• 제12권2호
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• pp.117-121
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• 2014

본 시험은 팽이버섯 병재배용 배지의 혼합수준에 따른 전탄소(T-C), 전질소(T-N) 함량과 균배양기간, 버섯발생기간, 자실체 수량 등 재배적 특성을 조사하였다. 배지재료의 혼합비율을 달리하였을 때 팽이버섯의 경우 [콘코브75+미강20+건비지5] 배지에서 자실체 수량이 190.5 g/850 ml로 가장 높았으나(20.4% 증가), 균 배양기간이 28일로 대조구보다 8일 지연되었다(대조구 [미송톱밥75+미강25]: 수량 158.2g, 배양기간 20일). [미송톱밥75+미강20+건비지5] 배지는 팽이버섯의 자실체 수량이 172.7 g(9.1% 증가), 균 배양기간이 21일로 대조구보다 1일 지연되었다.