• Journal of Wetlands Research
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• v.25 no.4
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• pp.257-266
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• 2023

Amphibian populations are declining globally, pushing many species to the brink of extinction. To promote biodiversity and sustainable management, countries are actively researching amphibian reproductive ecology. Sperm cryopreservation is a crucial assisted reproductive technology that aids in preserving the genetic diversity of amphibians. However, because amphibian sperm cells are sensitive to osmotic stress, the optimal cryopreservation method therefore differs from species to species. This literature review offers an overview of the significance of developing cryopreservation techniques for amphibian conservation and highlights the need to create optimal cryopreservation methods and the introduction of long-term monitoring (e.g., fertilization success and offspring reproduction) to advance cryopreservation technology development. This review can be used as basic research data for amphibian conservation methods.