• Title/Summary/Keyword: crude drug preparation (So-Shi-Ho-Tang)

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Identification and Quantitative Determination of Index Component of Glycyrrhizae Radix from Crude Drug Preparation Containing Bupleuri Radix (시호(柴胡) 함유(含有) 생약제제(生藥製劑)중 감초(甘草) 지표성분(指標成分)의 확인(確認) 및 정량(定量))

  • Choi, Kang-Ju;Ko, Sung-Ryong;Jeon, Byeong-Seon
    • Korean Journal of Pharmacognosy
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    • v.20 no.4
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    • pp.227-232
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    • 1989
  • As a part of studies on the quality control of crude drug preparation (So-Shi-Ho-Tang), index components of Glycyrrhizae Radix were identified by TLC and quantified by HPLC. Specific red spot (Rf=0.47) was identified in acid hydrolysate of glycosidic fraction on silica gel plate with benzene/ethyl acetate (1 : 1, v/v). The content of glycyrrhizin was determined by quantification of glycyrrhetinic acid by HPLC on ${\mu}-Bondapak\;C_{18}$ column with $MeOH/H_2O/HAc$ (78 : 19 : 3, v/v). Its recovery rate in the extract granules, compared to the content in the Glycyrrhizae Radix, was $83.3{\pm}0.7%$.

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Identification of Index Components of Scutellariae Radix and Quantitative Determination of Baicalin from Curde Drug Preparation Containing Bupleuri Radix (시호(柴胡)함유 한방제(漢方劑)중 황금(黃芩) 지표성분(指標成分)의 확인(確認) 및 Baicalin 정량(定量))

  • Choi, Kang-Ju;Ko, Sung-Ryong;Yang, Jae-Won
    • Korean Journal of Pharmacognosy
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    • v.21 no.2
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    • pp.158-162
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    • 1990
  • As a part of studies on the quality control of crude drug preparation, So-Shi-Ho-Tang, index components of Scutellariae Radix were identified by TLC and quantified by HPLC. Wogonin and baicalein were identified in ethyl ether fraction on silica gel plate with benzene/ethyl acetate (1 : 1, v/v). The content of baicalin was determined by HPLC on ${\mu}-Bondapak\;C_18$ column with 0.5%-phosphoric acid/acetonitrile (73 : 27, v/v). Its recovery rate in the extract granules, compared to the content in the Scutellariae Radix, was $52.1\;{\pm}\;0.7%$.

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IDENTIFICATION AND DETERMINATION OF GINSENG SAPONINS, PROSAPOGENINS AND SAPOGENINS FROM CRUDE DRUG PREPARATIONS FOR QUALITY CONTROL

  • Choi Kang Ju;Ko Sung Ryong;Kim Seok Chang;Kim Man Wook
    • Proceedings of the Ginseng society Conference
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    • 1993.09a
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    • pp.206-214
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    • 1993
  • Ginseng saponins have been known as main active principles and analyzed as the index components in ginseng and its products for quality control. But it is generally difficult to analyze the saponins in crude drug preparations. Saponins, Prosapogenins and sapogenins of crude drug preparation were identified by TLC and determined quantitatively by HPLC. $Prosapogemins-Rg_3\;-Rg_2\;and\;{\Delta}^{20}-prosapogenin$ were extracted with ethyl acetate from $50\%$ acetic acid hydrolyzates of saponin fractions and identified by TLC with lower phase of $CHCl_3/MeOH/H_2$ O\65:35:10. v/v)on silica gel plate, and quantified by HPLC on $Lichrosorb-NH_2$ column with $CH_3CN/H_2O(90:10,\;v/v).$ Sapogenins. panaxadiol and panaxatriol. were extracted with ethyl ether from $7\%-sulfuric$ acid hydrolyzates of saponin fractions and identified by TLC with chloroform/acetone(1 : 1 v/v) on silica gel plate. and quantified by HPLC on u - Bondapak $C^{18}$ column with $CH_3CN/MeOH/CHCl_3(83:10:7.\;v/v).$ These analyses of prosapogenins and sapogenins are more useful for quality control than those of saponins in crude drug preparations such as So - Shi - Ho - Tang(소시호탕), Sa - Kun - Ja - Tang(사군자탕), Yook - Kun - Ja - Tang(육군자탕), and In - Sam -Tang(인삼탕)

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