• 한국해양생명과학회지
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• 제7권2호
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• pp.187-195
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• 2022

붕어(무게 28.1±3.7 g, 길이 10.0±1.0 cm)에 0, 2.0×10⁴, 2.0×10⁵, 2.0×10⁶, 2.0×10⁷ CFU/ml의 Aeromonas hydrophila를 주사 후 2주간 실험을 실시하였다. A. hydrophila로 인위감염한 Carassius carassius의 2주 뒤 반수치사농도는 19.776×10⁵ CFU/ml이었다. 혈액학적 지표에서는 A. hydrophila에 의해 혈색소와 적혈구 수가 유의하게 감소한 반면, 적혈구 용적은 유의적 변화가 없었다. 마그네슘, 칼슘과 같은 혈장 무기 성분은 유의적으로 감소하였다. 유기 혈장 성분에서 A. hydrophila 인위감염에 의해 혈장 포도당과 콜레스테롤이 유의하게 증가한 반면, 총 단백질은 유의하게 감소하였다. 혈장 효소 성분인 ALP는 A. hydrophila 인위감염에 의해 유의하게 증가하였다. 본 연구의 결과는 C. carassius에 대한 A. hydrophila의 인위감염이 치명적인 병원성 세균으로 혈액학적 성상 및 혈장 성분에 유의한 생리학적 변화를 유도하였음을 의미한다.

• 한국발생생물학회지:발생과생식
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• 제21권1호
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• pp.55-62
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• 2017

We used flowcytometry to ploidy verification after that investigate difference between diploid and spontaneous triploid through the truss dimension and classical dimension at crucian carp, Carassius auratus, crucian carp C. cuvieri and common carp, Cyprinus carpio collected from Hangang river, Hantangang river, Imjingang river, Geumgang river, Yeongsangang river and Nakdonggang river, Korea. There were significant differences among the three species for the truss dimensions anterior origin of dorsal fin $(2){\times}$ anterior origin of anal fin (5), $2{\times}$ anterior origin of pelvic fin (6), $2{\times}$ origin of pectoral fin (7), posterior origin of dorsal fin $(3){\times}5$, $3{\times}6$, and $3{\times}7$ (P<0.05). There were no significant differences among the three species in the truss dimensions dorsal fin length ($2{\times}9$) and eye diameter (ED)(P>0.05). On the other hand, there were no significant differences in the several classical dimensions of each species (P>0.05). Three classical dimensions, most anterior extension of the head $(1){\times}2$, $1{\times}6$ and $2{\times}$ most posterior scale in lateral line (4) did not differ between the C. auratus diploid and spontaneous triploid (P>0.05). Two classical dimensions, $1{\times}6$ and longest length between most anterior extension of the head and gill cover ($1{\times}8$) did not differ between the C. cuvieri diploid and spontaneous triploid (P>0.05). One classical dimensions, $1{\times}2$ did not differ between the common carp diploid and spontaneous triploid (P>0.05). There were significant differences in the each diploid and triploid species (P<0.05). These results suggest that the classification of each species and classification between diploid and spontaneous triploid morphometrical parameters used in this study are useful indices of morphometrical status in the each species from major river of Korea.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2002년도 춘계학술대회(Genomics, Proteomics, and Risk Assesment):환경독성 및 위해성 평가 국제 심포지움
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• pp.67-67
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• 2002

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.174-174
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• 2002

Metallothioneins (MTs) are known to be induced by heavy metals in various organs of different species and represent a potential biomarker of aquatic contamination by heavy metals. In this work, cloning and sequencing of a metallothionein gene in crucian carp (Carassius auratus) was done and sensitivities and specificities of the gene expressions were compared.(omitted)

• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7879-7884
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• 2014

To investigate the association between intake of freshwater fish and their fatty acids and the risk of breast cancer in Chinese women, we conducted a case-control study with 669 cases and 682 population-based controls in Jiangsu Province of China. A structured questionnaire was used to elicit detailed information. Unconditional logistic regression analysis was performed to calculate odds ratios (ORs) and 95% confidence intervals (CIs). Total freshwater fish intake was linked to decrease in the adjusted OR for breast cancer, but without dose-dependence. Analyses by freshwater fish species showed that consumption of black carp and silver carp was inversely related to breast cancer risk, with adjusted-ORs for the highest intake category of black carp (${\geq}500g/month$) of 0.54 (95%CI=0.33-0.92; $P_{trend}$ <0.002) and for silver carp (${\geq}1000g/month$) of 0.19 (95%CI=0.11-0.33; $P_{trend}$ <0.001). In contrast, consumption of crucian carp was positively related to breast cancer risk, with an adjusted OR for the highest intake category (${\geq}1000g/month$) of 6.09 (95%CI=3.04-12.2; $P_{trend}$ <0.001). Moderate intakes of SFA, PUFA, n3-PUFA and n6-PUFA from freshwater fish may decrease the risk of breast cancer among premenopausal women. The findings of this study suggest that intake of freshwater fish and their fatty acids may modify risk of breast cancer, and that different species of freshwater fish could have a different actions on breast cancer risk. Future epidemiologic studies are needed to know the effects of freshwater fish intake on breast cancer risk and the cause of these effects.

• 한국물환경학회지
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• 제29권5호
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• pp.656-665
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• 2013

This research was conducted to investigate pollutant load unit factor from aquacultural farms. Pollutant load unit factors were investigated on the 13 kinds of fish type, i.e., Trout, Leather Carp, Eel, Carp, Cartfish, Freshwater Eel, Crucian Carp, Colored Carp, Sturgeon, Marsh Snail, Sweetfish, Pond Snail, Eriocheir Sinensis. Water qualities in aquacultural farms were investigated wide range of values by fish type and pollutant items. High BOD and COD values were observed at the Leather Carp (Ponded water system), Cartfish (Ponded water system) and Freshwater Eel (Recirculating System). TOC and DOC values were relatively high at the Freshwater Eel (Recirculating System) and Eriocheir Sinensis (Extensive). Eel (Ponded water system) and Freshwater Eel (Recirculating System) produced high concentrated nutrient pollutants, i.e., T-N, T-P. Pollutant load unit factors are dependent on fish type, aquacultural type, water quality items, etc. If some fishes have similar basic unit values, those could be applied for TMDLs as a group of fish having same pollutant load unit. The water quality concentrations of post treatment facilities' (mainly reservoir tank) effluent were higher than those of fish raising bath because of extracting pollutant from sediment in the reservoir tank. Therefore, it needs to the management and regulations about post treatment facilities.

• Journal of Ecology and Environment
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• 제37권4호
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• pp.209-216
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• 2014

This study was undertaken to suggest an effective fisheries resources management system by using stock assessment and potential yield analyses of crucian carp population in the mid-upper system of the Seomjin River. Fieldwork was conducted seasonally from 2008 to 2009 in the mid-upper system of the Seomjin River. The stock assessment was carried out by the swept area method and the potential yield was estimated by improved fisheries resource potential estimation system based on the Allowable Biological Catch. Also, the yield-per-recruit analysis was used to review the efficient management implication of the resource, Carassius auratus. As a result, the age at first capture ($t_c$) was estimated as 1.468 year, converted body length (BL) was 10.8 cm. Meaning the current fishing intensities, the instantaneous coefficient of fishing mortality (F) was $0.067year^{-1}$, and the yield-per-recruit analysis showed that the current yield per recruit was estimated to be 15.999 g with F and $t_c$. The instantaneous rate of fishing mortality that provides for Allowable Biological Catch ($F_{ABC}$) based on the current $t_c$ and F was estimated as $0.618year^{-1}$. Therefore, the optimum fishing intensities could be achieved at the higher fishing intensity for Carassius auratus. The calculated annual stock of C. auratus was estimated as 7,608 kg, and the potential yield was estimated as 343 kg with $t_c$ and F at the fixed current level. Using yield-per-recruit analysis, if F and $t_c$ were set at $0.618year^{-1}$ and 2 year, the yield per recruit and total allowable catch would be predicted to increase to 62 g and 2,531 kg by about 3.9 times and 7.3 times, respectively.

• 한국양식학회지
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• 제8권3호
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• pp.171-181
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• 1995

붕어의 년 생식주기를 밝히기 위해 1993년 2월부터 1994년 10월까지 gonadosomatic index (GSI)를 조사하였다. GSI는 4월부터 7월까지는 높은 수준을 나타내며 개체간에 편차가 큰 것으로 보아 이 기간이 산란기임을 보여준다. 8월부터 9월까지는 년중에서 가장 낮은 수준이며 이때 난소내 여포는 퇴화가 진행 중이었다 10월부터 GSI 값은 증가하여 이듬해 3월에 최대치를 보였다. Human chorionic gonadotropin (HCG 10 lU), $17\alpha$, 20\beta-dihydroxyprogesterone\;(1-100{\mu}g/ml)$ 및 phorbol 12-myristate 13-acetate (TPA, protein kinase C activator, 0.1-10${\mu}M$)는 인공배양 시 난모세포의 성숙을 유도하였으나 $4\alpha-phorbol$ 12, 13-didicanoate ($4\alpha-PDD$, phorbol ester analogue, $(25{\mu}M$)는 성숙을 일으키지 않았다. 또한, HCG (10 IU), prostaglandin $F_{2\alpha}$ (0.1-10${\mu}g/ml$) 및 TPA (0.1-10${\mu}M$)는 난모세포의 배란을 유도하였으나 $4\alpha-PDD$$(25\;{\mu}M)$에 의해서는 배란이 일어나지 않았다. 여포세포의 $17\alpha-hydroxyprogesterone$은 HCG (1 IU, 10 IU) 및 forskolin (adenylate cyclase activator, 0.1-10 ${\mu}M$)에 의해 생성이 촉진되었으며 HCG (10 IU) 및 forskolin $(10 {\mu}M)$에 의한 time course 는 3시간 내에 생성량이 증가하여 시간경과에 따른 유의한 차이는 보이지 않았다. 이러한 결과를 종합하면 cyclic AMP와 protein kinase C 는 어류의 난모세포의 성숙과 배란과정에 매우 중요한 역할을 담당하는 것으로 생각된다.

• Ecology and Resilient Infrastructure
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• 제10권4호
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• pp.258-265
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• 2023

대부분의 어류가 생존을 위해 회유 또는 이동하는 특성이 있음에도 불구하고 국내에 설치된 어도는 평상시에 고유속과 저수심의 흐름이 발생할 수 있어 어류의 이동에 장애가 되고 있는 실정이다. 또한 어도에 관한 국내 설계기준상 국내 하천에 서식하는 다양한 어종별 유영특성을 고려하지 못하는 한계가 있다. 따라서 어도의 기능을 확보하기 위한 설계기준의 마련이 필요하며, 이를 위해 국내 회유성 어종의 유영특성과 어도의 수리특성에 대한 연구가 필요하다. 이에 본 연구에서는 붕어를 대상으로 증진유속방법과 고정유속방법을 수행하여 가능한 객관적인 어류의 유영특성을 분석하고 한계유영유속을 제시하였다. 증진유속방법 실험을 수행한 결과 붕어는 0.7 m/s ~ 0.8 m/s로 하는 것이 바람직한 것으로 확인되었다. 고정유속방법 실험을 수행한 결과 붕어의 경우는 0.8 m/s 정도로 확인되었다. 따라서 두 실험 방법을 종합적으로 분석한 결과 어도설계를 위한 한계유영유속을 결정하면 붕어의 경우는 약 0.8 m/s로 판단된다. 향후 다양한 회유성 어종에 대해 유영특성의 실험적 연구를 수행하여 어도설계를 위한 대상어종별 유영특성을 고려한 어도설계기준 마련이 필요하다.

• 한국어병학회지
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• 제34권2호
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• pp.133-140
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• 2021

With the recent isolation of a new betanodavirus in shellfish, Korean Shellfish Nervous Necrosis Virus (KSNNV), it has also been identified the reassortant KSNNV of two RNA segments, in which one segment is KSNNV genotype but the other one is known genotype. In this study, we confirmed that the ressortant KSNNVs obtained in previous screening study of our laboratory for betanodaviruses in shellfish were KS/RGNNV and RG/KSNNV type by performing two consecutive multiplex RT-PCR on each RNA1 and RNA2 segment (R1- and R2-discriminative multiplex two-step RT-PCR, respectively) to determine the genotype of each segment based on the size of amplicon. In the pathogenicity analysis, none of the reassortants induced specific external symptoms or mortality of VNN, but viruses of 2 × 10⁴~10⁵ copies/mg or more were detected at 14 days after injection (10⁷ copies/fish) in brain tissues of 4 species except for crucian carp and common carp among the 6 species of juvenile fish used. In addition, the histopathological features of weak but distinct vacuole formation were also found in the brain of these infected fish, but no difference was found between the two reassortants KS/RGNNV-KG and RG/KSNNV-CM.